<div><p><strong>In 2025, two outstanding young female researchers, Drs. Filipa Oliveira and Stella Gonsales, both originally from outside the Czech Republic, received prestigious JUNIOR STAR grants from the Czech Science Foundation (GAČR). JUNIOR STAR enables early career researchers to establish their own independent research groups and significantly advance their scientific careers. Their projects reflect cutting-edge research in materials science and sustainable chemistry, highlighting the international reach and welcoming research environment at UCT Prague.</strong></p><p><strong>Dr. Stella Gonsales is also a&nbsp;laureate of the Dagmar Procházková Fund, which supports promising scientific projects in sustainable and advanced organic chemistry. Another recipient of this grant is Dr.&nbsp;Anna Valunchene, whose research complements UCT Prague’s&nbsp;sustainability goals. This dual funding underlines Stella’s&nbsp;remarkable achievements and the wide scope of innovative research at UCT Prague.</strong></p><p><strong>Both Filipa and Stella are actively looking for motivated students and researchers to join their teams, offering a&nbsp;unique opportunity to work at the forefront of science in a&nbsp;vibrant and supportive environment.</strong></p></div><div><h3><strong>Interview with Filipa Oliveira: Pioneering tubular MXenes for supercapacitor energy storage</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Filipa Oliveira" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg 235w," sizes="100vw" width="1500" height="999"></p><h5><strong>Congratulations on receiving the JUNIOR STAR grant. What does this achievement mean for you and how will it influence your next steps?</strong></h5><p>Thank you. It’s&nbsp;a huge achievement to receive this prestigious grant. It gives me the opportunity to establish my own research group here at UCT Prague. The JUNIOR STAR program supports talented young scientists in building their teams and accessing resources. This means I&nbsp;now have the support to focus fully on my research about tubular MXenes and their application in supercapacitors.</p><h5><strong>Why are tubular MXenes so innovative for energy storage?</strong></h5><p>MXenes are a family of 2D materials with a layered, accordion-like structure and known for their excellent electrical conductivity. Our innovation is to produce them with a tubular morphology, combining the high conductivity and redox activity in superior ion-transport tubular architectures. Their open, hollow structure increases surface area, ion diffusion and charge transport. This could translate to faster, more efficient supercapacitors, critical for sustainable energy technologies.</p><h5><strong>How did your scientific interest lead you to this field?</strong></h5><p>Global challenges around green energy made me focus on materials that can improve energy storage. MXenes stood out because changes in the composition and structure can significantly change their properties. I&nbsp;was curious about how tuning different metallic elements in a&nbsp;tubular morphology can impact how MXenes perform and how to optimize them for practical applications.</p><h5><strong>What challenges have you encountered?</strong></h5><p>One major challenge is their stability: MXenes oxidize quickly which deteriorates performance. Another challenge is understanding how synthesis conditions of both MXenes and their precursors (MAX phases) must be adapted to create and preserve tubular morphologies since their synthesis mechanisms differ from conventional MXenes. Additionally, effectively communicating the project in the funding proposal to show to reviewers why I&nbsp;should obtain this funding was also a&nbsp;challenge, but thanks to mentorship and collaboration, I&nbsp;overcame these hurdles.</p><h5><strong>What motivates you most about working at UCT Prague?</strong></h5><p>The support from colleagues and the excellent technical infrastructure make it a&nbsp;great place to work. Also, being a&nbsp;former member of Professor Sofer’s&nbsp;leading group in 2D&nbsp;materials is inspiring and led me to this achievement.</p><h5><strong>How do&nbsp;you see your professional development going in the future?</strong></h5><p>The grant will help me build my own team, acquire equipment, and develop collaborations. My long-term vision is to contribute significantly to sustainable energy solutions and scientific progress both locally and internationally.</p><h5><strong>You are looking for new team members. What kind of students or scientists do&nbsp;you need?</strong></h5><p>We are currently welcoming enthusiastic master's&nbsp;students, PhD candidates, and postdocs who are passionate about materials science and eager to explore new frontiers in MXenes and energy storage. If you love curiosity-driven research and want to be part of a&nbsp;growing international team, we encourage you to join us.</p><h3><strong>Interview with Stella Gonsales: Catalysis as a&nbsp;key to polymer recycling</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Stella Gonsales" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg 233w," sizes="100vw" width="1499" height="1000"></p><h5><strong>Congratulations on your JUNIOR STAR grant and also being awarded the Dagmar Procházková Fund. What does receiving both mean to you and how will they shape your work?</strong></h5><p>Thank you. Both grants are hugely important. The JUNIOR STAR grant allows me to expand my group and resources for research into catalytic recycling of polymers, while the Dagmar Procházková Fund supports complementary projects such as synthesizing new biodegradable polymers. Together, they help tackle the plastic waste problem from multiple scientific angles.</p><h5><strong>Could you explain your research in layperson’s&nbsp;terms?</strong></h5><p>Most plastics have strong carbon-carbon bonds. We insert more cleavable double bonds into polymers that can be broken down by catalytic reactions called olefin metathesis, making recycling on a&nbsp;molecular level more feasible.</p><h5><strong>What makes the JUNIOR STAR grant particularly important for your research and career development?</strong></h5><p>The JUNIOR STAR grant is a&nbsp;prestigious and highly competitive funding opportunity aimed at excellent early-career scientists within eight years of completing a&nbsp;Ph.D. It provides up to 25&nbsp;million CZK over five years, allowing recipients to establish independent research groups and pursue innovative scientific topics. This grant is crucial for building a&nbsp;solid foundation, acquiring necessary equipment, and recruiting talented team members, enabling the pursuit of ambitious projects that can significantly contribute to both the Czech scientific community and global challenges.</p><h5><strong>What collaborations support your research?</strong></h5><p>The collaboration between UCT Prague and the Institute of Organic Chemistry and Biochemistry (IOCB) of the Czech Academy of Sciences primarily provides financial and technical support. The cooperation helps with funding, access to advanced equipment, and operational resources needed for running research projects.</p><h5><strong>What advice would you give young scientists aiming to succeed?</strong></h5><p>I&nbsp;encourage thinking outside the box and combining diverse knowledge you acquire during your career to address complex problems that are exciting and relevant to you.</p><h5><strong>Your laboratory bears the name of Otto Wichterle and was ceremonially opened in the presence of Professor Kamil Wichterle. How does Wichterle’s&nbsp;legacy inspire you personally?</strong></h5><p>Wichterle’<s>’</s>s&nbsp;innovative spirit motivates me to push creative boundaries and pursue novel solutions in sustainable chemistry.</p><h5><strong>What are your visions for the future?</strong></h5><p>I&nbsp;aim to build a&nbsp;research group that advances sustainable polymer recycling, contributing to environmental responsibility and the circular economy.</p><h5><strong>You are recruiting people for your team. What skills do&nbsp;they need to have?</strong></h5><p>We are looking for talented Ph.D. students and postdocs experienced in air-sensitive catalysis techniques. If you want to contribute to transformative research in sustainable chemistry and join a&nbsp;dynamic team, this is a&nbsp;great opportunity.</p></div>

JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science

News from LabRulezGCMS Library - Week 06, 2026

<p><strong>Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal </strong><i><strong>Nature Chemical Biology</strong></i><strong>.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.</p><p>In their study,<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en"><strong>Dr. Hana Cahová</strong></a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.</p><p>The work was significantly shaped by <strong>Valentina Serianni from Hana Cahová’s team</strong>, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by <strong>Jana Škerlová</strong>, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.</p><p>Hana Cahová adds: <i>“We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”</i></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by<strong> Dr. Tomáš Kouba</strong>, one of the authors of the study. <i>“Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,”</i> he explains.</p><p>Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en"><strong>cryo-EM center</strong></a><strong> </strong>that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"></p><blockquote><h4><strong>The Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5"><strong>Molecular insight into 5′ RNA capping with Np<sub>n</sub>Ns by bacterial RNA polymerase</strong></a></h4><p>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;</p><p>Nat. Chem. Biol. 21 (2026)</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from <i>Thermus</i> <i>thermophilus</i>. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</p></div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

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The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

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LECO Multi-Mode Source

Multi-Mode Source (MMS) integrates EI, PCI, and NCI/ECNI in a single GC-MS ion source, enabling instant software-based mode switching with perfect peak alignment and enhanced unknown identification.

<p><strong>The innovative Multi-Mode Source (MMS) integrates the strengths of the three most widely used GC-MS ionization techniques—Electron Ionization (EI), Positive Chemical Ionization (PCI), and Negative Chemical Ionization (NCI/ECNI)—into a single, easy-to-use source. With MMS, switching ionization modes is software-controlled and instantaneous, eliminating hardware changes and alignment concerns.</strong></p><p>Paired with the <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/38"><strong>Pegasus GC-HRT+</strong></a>, MMS delivers excellent mass accuracy and high resolution for pseudo-molecular ions, complementing traditional high-resolution EI (HR-EI) to enable comprehensive characterization of unknown compounds. Because the hardware remains untouched during mode changes, chromatographic peaks stay perfectly aligned across ionization modes—simplifying comparison and interpretation.</p><p>All three ionization modes (EI, PCI, NCI/ECNI) are available for both <strong>GC-MS</strong> and <strong>GC×GC-MS</strong> workflows on the <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/39"><span><strong>Pegasus GC-HRT+ 4D</strong></span></a> as well as the Pegasus GC-HRT+ systems.</p><h3><strong>Key Benefits</strong></h3><ul><li><strong>Three ionization modes in one source</strong>: EI, PCI, and NCI/ECNI</li><li><strong>No hardware swapping or realignment</strong>: mode changes at the click of a button</li><li><strong>Consistent peak alignment</strong> across ionization modes</li><li><strong>Enhanced unknown identification</strong> via complementary EI and CI information</li><li><strong>Compatible with GC-MS and GC×GC-MS</strong> on Pegasus GC-HRT+ platforms</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_Multi_Mode_Source_2_79908249a5/LECO-Multi-Mode-Source-2.jpg" alt="LECO: Multi-Mode Source for the Pegasus GC-HRT+ and Pegasus GC-HRT+ 4D" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_Multi_Mode_Source_2_79908249a5/thumbnail_LECO-Multi-Mode-Source-2.jpg 245w," sizes="100vw" width="1817" height="1082"></p>

LECO PEGASUS BT GC-TOFMS

Compact GC-TOFMS delivering full mass range data in every run, high sensitivity, NonTarget Deconvolution® and StayClean® EI source for reliable non-target and routine GC-MS analysis.

<p><strong>The Pegasus BT GC-TOFMS is a compact, high-performance benchtop gas chromatography time-of-flight mass spectrometer designed to deliver comprehensive chemical information with exceptional sensitivity, speed, and reliability. It combines advanced TOF mass spectrometry hardware with LECO’s powerful </strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/41"><strong>ChromaTOF® software</strong></a><strong> to support both routine and advanced analytical workflows.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_Pegasus_BT_2_bc8a8d0505.jpg" alt="LECO PEGASUS BT GC-TOFMS" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_Pegasus_BT_2_bc8a8d0505.jpg 238w," sizes="100vw" width="932" height="612"></p><p>Designed for laboratories that need to analyze more samples, generate more data, and achieve results faster, the Pegasus BT acquires full-range mass spectral data in every run—ensuring that no analytes are missed, even in highly complex matrices. Industry-leading sensitivity, combined with <strong>NonTarget Deconvolution® (NTD®)</strong>, enables confident identification and quantification of both known and unknown compounds by effectively separating co-eluting peaks and minimizing matrix interferences.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_PEGASUS_BT_GC_TOFMS_7_be16be31ec.jpg" alt="LECO PEGASUS BT GC-TOFMS: NonTarget Deconvolution® (NTD®) Automated deconvolution ensures that coeluting peaks and matrix interferences don't hinder your analyses.StayClean Ion Source Open-style EI source virtually eliminates downtime associated with source cleaning*, which means you can spend your time running more samples.  *Even after 3,000 sludge injections, source has not needed cleaning." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_PEGASUS_BT_GC_TOFMS_7_be16be31ec.jpg 245w," sizes="100vw" width="768" height="432"></p><p>The system features LECO’s proven <strong>StayClean® open-style EI ion source</strong>, which virtually eliminates downtime associated with source cleaning and allows extended operation even with challenging sample matrices. Its compact benchtop footprint makes the Pegasus BT an ideal solution for laboratories with limited space, without compromising analytical performance.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_PEGASUS_BT_GC_TOFMS_6_047f81e642.jpg" alt="LECO PEGASUS BT GC-TOFMS: " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_PEGASUS_BT_GC_TOFMS_6_047f81e642.jpg 245w," sizes="100vw" width="616" height="375"></p><p>Fully integrated <strong>ChromaTOF® software</strong> provides automated deconvolution, quantification, reporting, batch processing, and long-term data mining. The Pegasus BT GC-TOFMS is ideally suited for pesticide analysis, metabolomics, petroleum and fuel characterization, food, flavor, and fragrance analysis, and other demanding GC-MS applications.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_PEGASUS_BT_GC_TOFMS_2_cc1a963b9e.jpg" alt="LECO PEGASUS BT GC-TOFMS: ChromaTOF® software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_PEGASUS_BT_GC_TOFMS_2_cc1a963b9e.jpg 245w," sizes="100vw" width="943" height="566"></p><h3><strong>Key Features</strong></h3><ul><li><strong>Benchtop GC-TOFMS</strong> with full-range mass spectral acquisition in every analysis</li><li><strong>Industry-leading sensitivity</strong> for trace-level detection</li><li><strong>NonTarget Deconvolution® (NTD®)</strong> for reliable separation of co-eluting compounds and suppression of matrix interferences</li><li><strong>StayClean® open-style EI ion source</strong> for minimal maintenance and reduced downtime</li><li><strong>High sample throughput with excellent repeatability</strong></li><li><strong>Compact footprint </strong>with uncompromised analytical performance</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_PEGASUS_BT_GC_TOFMS_5fd6d4a332.jpg" alt="LECO PEGASUS BT GC-TOFMS" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_PEGASUS_BT_GC_TOFMS_5fd6d4a332.jpg 245w," sizes="100vw" width="1220" height="591"></p><h3><strong>Applications</strong></h3><ul><li>Pesticides and environmental contaminants</li><li>Petroleum products, fuels, and fuel marker analysis</li><li>Metabolomics and non-target screening</li><li>Food, flavor, and fragrance analysis</li><li>Characterization of complex matrices and unknown identification</li></ul><h5><strong>&nbsp;Calibration curve in strawberry matrix: Cyprodinil</strong></h5><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_PEGASUS_BT_GC_TOFMS_3_6a4f0ecc50.jpg" alt="LECO PEGASUS BT GC-TOFMS: The calibration curve at left shows excellent linearity, and sensitivity easily meets all standard requirements." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_PEGASUS_BT_GC_TOFMS_3_6a4f0ecc50.jpg 245w," sizes="100vw" width="871" height="480"></p><h5><strong>Characterization of fuel markers at trace levels is easy and routine</strong></h5><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_PEGASUS_BT_GC_TOFMS_4_33217d7f2e.jpg" alt="LECO PEGASUS BT GC-TOFMS: Full TIC chromatogram of a spiked commercial diesel sample." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_PEGASUS_BT_GC_TOFMS_4_33217d7f2e.jpg 237w," sizes="100vw" width="742" height="489"></p>

LECO PEGASUS BT 4D GC×GC-TOFMS

LECO PEGASUS BT 4D GC×GC-TOFMS combines comprehensive two-dimensional GC with fast TOFMS to improve separation, sensitivity, and confidence in qualitative and quantitative analysis of complex samples.

<p><strong>The LECO PEGASUS BT 4D GC×GC-TOFMS is a high-performance benchtop system that combines comprehensive two-dimensional gas chromatography (GC×GC) with time-of-flight mass spectrometry (TOFMS) to deliver unmatched separation power, sensitivity, and confidence in compound identification. Designed for laboratories facing highly complex samples, the PEGASUS BT 4D enables qualitative and quantitative analysis in a single run while maximizing productivity and data quality.</strong></p><p>By coupling GC×GC separation with fast TOFMS acquisition, the system <strong>dramatically increases chromatographic peak capacity and resolves co-eluting compounds </strong>that are difficult or impossible to distinguish by conventional 1D GC-MS. Enhanced signal-to-noise ratios translate into lower detection limits, improved linearity, and more reliable quantification—even in challenging matrices.</p><h3><strong>What GC×GC Brings to Your Analysis</strong></h3><p>GC×GC modulation refocuses and reinjects analytes from the first column into a second column with a different stationary phase, providing structured chromatograms that improve interpretability of complex data. This approach improves both separation efficiency and interpretability of results, allowing you to identify more compounds with greater confidence and consistency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_PEGASUS_BT_4_D_G_Cx_GC_TOFMS_c9ad0bd186.jpg" alt="LECO PEGASUS BT 4D GC×GC-TOFMS" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_PEGASUS_BT_4_D_G_Cx_GC_TOFMS_c9ad0bd186.jpg 245w," sizes="100vw" width="1029" height="594"></p><h3><strong>Confident Identification and Quantification</strong></h3><p>The PEGASUS BT 4D excels in both comprehensive screening and targeted applications. Spectral deconvolution combined with high-speed TOFMS acquisition reveals analytes present in complex matrices, while GC×GC separation minimizes matrix interferences for superior quantitative performance. What was once a marginal library match in GC-MS becomes a confident identification in GC×GC-TOFMS.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_PEGASUS_BT_4_D_G_Cx_GC_TOFMS_2_4e033e866c.jpg" alt="LECO PEGASUS BT 4D GC×GC-TOFMS: Quantitative analysis using the Pegasus BT 4D" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_PEGASUS_BT_4_D_G_Cx_GC_TOFMS_2_4e033e866c.jpg 245w," sizes="100vw" width="1443" height="637"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_PEGASUS_BT_4_D_G_Cx_GC_TOFMS_3_18eebd600d.jpg" alt="LECO PEGASUS BT 4D GC×GC-TOFMS: Qualitative analysis using the Pegasus BT 4D" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_PEGASUS_BT_4_D_G_Cx_GC_TOFMS_3_18eebd600d.jpg 210w," sizes="100vw" width="1442" height="1069"></p><h3><strong>Robust Design for High-Throughput Labs</strong></h3><p>Equipped with LECO’s proven StayClean® ion source, the PEGASUS BT 4D minimizes routine maintenance and downtime, even when analyzing dirty or complex samples. Integrated with <strong>ChromaTOF®</strong> software, the system provides seamless instrument control, automated data processing, and powerful qualitative and quantitative tools within a single platform.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_PEGASUS_BT_4_D_G_Cx_GC_TOFMS_c2644cd38e.jpg" alt="LECO - PEGASUS BT 4D GCxGC-TOFMS.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_PEGASUS_BT_4_D_G_Cx_GC_TOFMS_c2644cd38e.jpg 202w," sizes="100vw" width="1099" height="849"></p><h3><strong>Key Benefits</strong></h3><ul><li>Exceptional chromatographic resolution and peak capacity with GC×GC</li><li>Enhanced sensitivity and lower detection limits through modulation-based signal focusing</li><li>High-speed TOFMS acquisition for accurate deconvolution of narrow GC×GC peaks</li><li>Reliable identification and quantification of analytes</li><li>Reduced maintenance with the StayClean® ion source</li><li>Fully integrated ChromaTOF® software for acquisition, processing, and reporting</li></ul><h3><strong>Applications</strong></h3><p>The LECO PEGASUS BT 4D GC×GC-TOFMS is ideally suited for demanding applications across environmental analysis, petrochemical and energy research, food and flavor/fragrance analysis, forensics, life sciences, and metabolomics—anywhere complex mixtures require maximum separation power and analytical confidence.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_PEGASUS_BT_4_D_G_Cx_GC_TOFMS_4_8e8ec115ec.jpg" alt="LECO PEGASUS BT 4D GCxGC-TOFMS: Excellent Sensitivity" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_PEGASUS_BT_4_D_G_Cx_GC_TOFMS_4_8e8ec115ec.jpg 244w," sizes="100vw" width="974" height="625">With the PEGASUS BT 4D, you can <strong>predict, quantify, and identify more compounds with confidence</strong>, turning complex data into clear, actionable results.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_PEGASUS_BT_4_D_G_Cx_GC_TOFMS_5_625fed2f0d.jpg" alt="LECO PEGASUS BT 4D GCxGC-TOFMS: Identify MORE with confidence/non-target analysis" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_PEGASUS_BT_4_D_G_Cx_GC_TOFMS_5_625fed2f0d.jpg 240w," sizes="100vw" width="1034" height="672"></p>

LECO Pegasus GC-HRT+

Ultra-high-resolution GC-TOFMS with up to 50,000 FWHM, <1 ppm mass accuracy, and 200 spectra/s. Pegasus GC-HRT+ delivers unmatched sensitivity and confident identification in complex samples.

<p><strong>Now with even higher sensitivity, the Pegasus GC-HRT+ is designed to meet the most demanding analytical challenges. This high-performance GC-TOFMS delivers industry-leading mass accuracy, full-range acquisition at exceptional speed, precise isotopic abundance, and ultra-high mass resolution—all within a single injection.</strong></p><p>Folded Flight Path (FFP) technology combined with a novel data acquisition system enables up to <strong>50,000 FWHM resolution</strong>, <strong>&lt;1 ppm mass accuracy</strong>, and <strong>acquisition rates up to 200 spectra/s</strong>, supporting rich analyte discovery and high-confidence identification in highly complex samples.</p><h3><strong>Key features</strong></h3><ul><li><strong>Mass accuracy ≤1 ppm</strong> with peak capacity at least <strong>2× higher</strong> than conventional MS platforms</li><li><strong>Encoded Frequent Pushing (EFP)</strong> for markedly increased sensitivity, expanded dynamic range, and wide mass range</li><li><strong>Folded Flight Path (FFP)</strong> technology for ultra-high resolution, enhanced sensitivity, and advanced TOF focusing</li><li><strong>KADAS®</strong> ion-statistics-based acquisition to reduce noise, ensure mass accuracy, and minimize file size</li><li><strong>Ultra High Resolution + HRD</strong> to resolve highly isobaric ions (mass differences ~0.02 amu)</li><li><strong>Multi-Mode Source (MMS):</strong> EI, Positive CI, and ECNI in one system</li></ul><h3><strong>Models</strong></h3><ul><li><strong>Pegasus GC-HRT+</strong> – Full-scale GC-TOFMS</li></ul><h3><strong>Options</strong></h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/82"><strong>L-PAL3 Automated Sample Injector</strong></a></li></ul>

LECO Pegasus GC-HRT+ 4D

High-resolution GC×GC-TOFMS system combining extreme separation power, up to 200 spectra/s acquisition, and advanced HR deconvolution for confident identification of unknowns in complex samples.

<p><strong>The Pegasus GC-HRT+ 4D from </strong><span><strong>LECO</strong></span><strong> combines comprehensive two-dimensional gas chromatography (GC×GC) with high-resolution time-of-flight mass spectrometry (TOFMS) and advanced High-Resolution Deconvolution (HRD). Designed for the most complex sample matrices, the system enables detection, separation, and confident identification of more analytes than ever before. </strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/41"><strong>ChromaTOF® software</strong></a><strong>, purpose-built for HRT+ instrumentation and GC×GC workflows, integrates advanced qualitative and quantitative tools into a single, user-friendly data system.</strong></p><h3><strong>Key Features</strong></h3><ul><li><strong>Compact benchtop design</strong> that saves valuable laboratory space</li><li><strong>Industry-leading sensitivity</strong> with proprietary deconvolution algorithms for clean, high-quality spectra</li><li><strong>High acquisition speed</strong> up to 200 spectra/s to properly define ultra-narrow GC×GC peaks (&lt;200 ms)</li><li><strong>Complete historical mass-spectral record</strong> retained for future data mining and reprocessing</li><li><strong>Proven Pegasus reliability and durability</strong> in a modern, space-efficient format</li></ul><h3><strong>GC×GC Performance Explained</strong></h3><p>GC×GC achieves exceptionally high peak capacity by trapping compounds eluting from the first column and reinjecting them into a second column for rapid secondary separation. These second-dimension separations occur within seconds, producing extremely narrow peaks that demand very high spectral acquisition rates. The Pegasus GC-HRT+ 4D meets and exceeds this requirement, enabling robust spectral deconvolution and confident compound identification in highly complex samples.</p><h3><strong>Available Configurations</strong></h3><ul><li><strong>Pegasus GC-HRT+ 4D with Liquid Nitrogen Thermal Modulator</strong></li><li><strong>Pegasus GC-HRT+ 4D with Cryogen-Free Modulator</strong></li></ul><h3><strong>Optional Accessories</strong></h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/82"><strong>L-PAL3 Autosampler</strong></a></li></ul>

LECO Chemical Ionization Source

Accurate-mass chemical ionization (HR-CI) for the Pegasus GC-HRT+ enables confident molecular ion confirmation, improved EI library matches, and reliable elemental formula generation for unknown compounds.

<p><strong>Confirm your unknowns with accurate mass chemical ionization (HR-CI), a new option for the </strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/38"><strong>Pegasus GC-HRT+</strong></a><strong>. The Pegasus GC-HRT+ is available with both electron ionization (EI) and HR-CI sources, offering you the option of comparison with classical library spectra (EI) and superior preservation of the molecular ion (HR-CI).</strong></p><h3><strong>Benefits of Positive ion Chemical Ionization</strong></h3><ul><li>Confirmation of molecular ions</li><li>More confidence in EI library matches</li><li>Propose chemical formulae for unknown or novel compounds</li><li>Reduce possible formulae with accurate mass data</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_Chemical_Ionization_Source_bb19256163.jpg" alt="LECO Chemical Ionization Source" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_Chemical_Ionization_Source_bb19256163.jpg 245w," sizes="100vw" width="1696" height="644"></p><p>The spectral transformations illustrated above compare and - . The left side is an example from a mouse liver extract,EI HR CI where soft ionization allows for confident confirmation of a marker in glycemic variability for individuals with type 2 diabetes. The right side demonstrates how - is ideal for forensic drug analysis, as drugs are constantly evolving andHR CI often not present in commercially available libraries.</p><h3><strong>Standard Features</strong></h3><ul><li>Benzophenone 1 pg sensitivity</li><li>Automated reagent gas flow optimization</li><li>Support for standard CI gases: methane, isobutane, and ammonia, PLUS “choose your own”</li><li>Uncompromised MS performance: speed, accuracy and resolution all at the same time, regardless of intensity</li></ul>

Products from category Mass detectors distributed by LECO Instrumente Plzeň - page 1

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