<p><strong>🎤Francis Femi Oloy</strong></p><p><strong>In this episode of Concentrating on Chromatography, David speaks with Francis Femi Oloy about using chromatography to uncover hidden pollutants in real-world water systems.</strong></p><p>Femi’s team analyzed polychlorinated biphenyls (PCBs) in six major rivers in southwestern Nigeria — compounds that were banned decades ago but still persist in the environment. Using a workflow that many analytical labs will recognize — liquid–liquid extraction, cleanup, rotary evaporation, nitrogen blowdown, and GC-ECD detection — they quantified 25 PCB congeners at trace levels and linked the results to ecological and human health risk.</p><h4><strong>In this conversation, we cover:</strong></h4><ul><li>Why legacy pollutants like PCBs still show up today</li><li>Choosing GC-ECD vs LC-MS for halogenated compounds</li><li>Liquid–liquid extraction and matrix cleanup strategies</li><li>Why sample concentration is critical for dilute environmental samples</li><li>How rotovap + nitrogen blowdown work together without losing volatile analytes</li><li>Seasonal trends (why wet season levels were higher)</li><li>Translating concentration data into meaningful risk assessments</li></ul><h3><strong>Video Transcription</strong></h3><section><div><div><div><div><div><div><p><strong>Persistent organic pollutants</strong> such as polychlorinated biphenyls (<strong>PCBs</strong>) remain a significant environmental concern despite decades of regulatory restrictions. In this interview, Francais Femi Oloy discusses the environmental and analytical challenges associated with monitoring PCB contamination in rivers of southwestern Nigeria, a rapidly industrializing region where urban growth, industrial activity, agriculture, and dense populations intersect with extensive aquatic systems.</p><p>Oloy explains that his interest in environmental contaminant analysis emerged from observing unexplained health problems and environmental degradation while growing up in Nigeria. This motivated his transition into organic and environmental chemistry, with a focus on identifying pollutants present in drinking water, agricultural soils, and food systems. Southwestern Nigeria, particularly the Lagos region, represents a critical environmental monitoring area due to intense industrial development, shipping activity, and widespread human dependence on rivers for domestic use, agriculture, and fishing.</p><p>Although PCBs are considered “<strong>legacy pollutants</strong>” and are no longer intentionally produced, the interview highlights why they continue to persist in aquatic environments. Old electrical transformers and aging industrial equipment still contain PCB residues, while historical contamination stored in river sediments can become remobilized during flooding, dredging, or sediment disturbance. Because PCBs are hydrophobic and resistant to degradation, they preferentially accumulate in sediments and bioaccumulate through aquatic food chains, creating long-term environmental and human exposure risks.</p><p>A major focus of the discussion centers on the analytical workflow used for PCB determination in environmental water samples. Composite river water samples were collected in amber glass bottles to avoid contamination and photodegradation, preserved with sodium azide to minimize microbial activity, and stored under cooled conditions prior to extraction. The analytical protocol employed <strong>liquid–liquid extraction</strong> using dichloromethane (DCM) and hexane to recover both polar and nonpolar PCB congeners. Oloy emphasizes that liquid–liquid extraction was selected over solid-phase extraction (SPE) because of concerns about congener retention within sorbent materials and clogging issues associated with large-volume environmental samples.</p><p>Following extraction, concentration and cleanup represented critical analytical stages due to the trace-level occurrence and volatility of PCBs. Silica and alumina cleanup procedures were used to remove matrix interferences prior to instrumental analysis. The concentration workflow combined rotary evaporation for bulk solvent reduction with nitrogen blowdown for final volume reduction to less than 1 mL, while carefully avoiding excessive temperature or complete dryness to minimize analyte loss. Oloy repeatedly stresses that improper concentration procedures are among the most common causes of analytical failure in PCB analysis.</p><p>For instrumental determination, the study utilized <strong>gas chromatography coupled with electron capture detection (GC-ECD)</strong> rather than LC-MS or GC-MS. According to Oloy, GC was preferred because PCBs are sufficiently volatile for gas chromatographic separation, while ECD provided superior sensitivity and selectivity for chlorinated compounds at environmentally relevant concentrations. The interview also discusses analytical validation strategies, including the use of surrogate standards to assess <strong>recoveries,</strong> which ranged from approximately <strong>85% to 114%</strong>, and signal-to-noise-based determination of detection limits.</p><p>The study further examined <strong>seasonal variations in PCB concentrations</strong>, revealing <strong>higher levels during the wet season</strong>. This observation was attributed to rainfall-driven runoff from contaminated infrastructure, sediment resuspension, and lower temperatures that reduce volatilization losses. To communicate toxicological relevance beyond simple concentration reporting, the researchers also calculated <strong>toxic equivalency values (TEQs)</strong>, converting mixtures of PCB congeners into biologically meaningful toxicity estimates comparable to dioxin toxicity metrics.</p><p>Beyond environmental monitoring, Oloy highlights broader research interests focused on <strong>pollutant remediation and sustainable environmental protection</strong>. His group is also involved in developing catalytic materials capable of degrading or removing persistent contaminants from environmental systems. Throughout the discussion, he emphasizes that analytical chemistry plays a central role not only in detecting pollutants, but also in enabling prevention, remediation, and long-term environmental management strategies.</p></div></div></div></div></div></div></section><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Tracking Toxic PCBs in River Water using Gas Chromatography–Electron Capture Detection

<p><strong>This study presents a high-temperature GC-APCI-MS/MS method for detecting large polycyclic aromatic hydrocarbons in pyroplastics and environmental samples. The approach enables sensitive analysis of PAHs with molecular weights from 314–424 Da without extensive cleanup or fractionation.</strong></p><p>Application to pyroplastics from the M/V X-Press Pearl fire revealed substantially elevated levels of large PAHs compared to unburnt plastics. Several compounds, including 1,3,5-triphenylbenzene, emerged as promising markers for identifying pyroplastic contamination, supporting future monitoring of plastic burning, fires, and microplastic pollution.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c11703"><strong>Gas Chromatography-Atmospheric Pressure Chemical Ionization (GC-APCI) Expands the Analytical Window for Detection of Large PAHs (≥24 Ringed-Carbons) in Pyroplastics and Other Environmental Matrices</strong></a></h4><p>Cara Megill, Douglas M. Stevens*, Christopher M. Reddy, Bryan D. James*, Robert K. Nelson, and Frank L. Dorman</p><p>ACS Omega 2026, 11, 7, 12321–12329</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acsomega.5c11703">https://doi.org/10.1021/acsomega.5c11703</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><div><p>The measurement and reporting of polycyclic aromatic hydrocarbons (PAHs) are routine practices in environmental monitoring and risk assessment. <a href="javascript:void(0);">(1−3)</a> However, of the thousands of PAHs, only the 16 designated as Priority Pollutants by the U.S. Environmental Protection Agency (EPA16) are typically reported. <a href="javascript:void(0);">(4,5)</a> These span two to six ringed parent PAHs, ranging in molecular weight from 128 to 278 Da. It is well recognized that this limited analyte list drastically simplifies and misses a significant fraction of PAHs. <a href="javascript:void(0);">(4)</a> For example, the concentration of C<sub>1</sub>–C<sub>4</sub> alkylated PAHs can be greater than or equal to the concentration of the EPA16 in crude oils and fuels. <a href="javascript:void(0);">(6)</a> Expanding the analytical window of PAHs has proven valuable for forensic analysis of oil spills (e.g., alkylated and heterocycle-containing PAHs) and relevant for toxicological evaluation(e.g., oxygen-, nitrogen-, and sulfur-containing PAHs). <a href="javascript:void(0);">(3,7)</a> Moreover, the sources of PAHs have distinct compositions of PAHs, <a href="javascript:void(0);">(1,8)</a> which enables the use of PAHs for source apportionment. <a href="javascript:void(0);">(2,7)</a> Including alkylated and sulfur-containing PAHs (those beyond the EPA16) in the investigation of environmental samples and other matrices provides greater discriminating power between potential sources (using extracted ion chromatograms). <a href="javascript:void(0);">(3,7,9)</a> Additionally, monitoring the compositional changes of PAHs in the environment can inform about the fate and environmental processing of the matrix (e.g., oil weathering). <a href="javascript:void(0);">(2)</a> While these efforts have made strides in considering other PAHs, an environmentally relevant fraction of PAHs remains underutilized.</p><p>PAHs with more than six rings have been reported in diverse environmental matrices. <a href="javascript:void(0);">(10,11)</a> These large PAHs (≥24 ringed-carbons <a href="javascript:void(0);">(10)</a>) have been found in combustion-derived particulate matter, <a href="javascript:void(0);">(12−19)</a> urban dust, <a href="javascript:void(0);">(20−23)</a> refinery deposits, <a href="javascript:void(0);">(11)</a> coal tars and pitches, <a href="javascript:void(0);">(24−27)</a> cokes, <a href="javascript:void(0);">(28)</a> soils, <a href="javascript:void(0);">(20,22)</a> sediments, <a href="javascript:void(0);">(20−23)</a> and deep-sea hydrothermal vent bitumen. <a href="javascript:void(0);">(29)</a> Most reports of large PAHs in environmental matrices have been from analyses of U.S. National Institute of Standards and Technology (NIST) standard reference materials (SRMs); <a href="javascript:void(0);">(18,21−23,25,26,30)</a> a few 302 Da isomers have been certified in three SRM. <a href="javascript:void(0);">(31)</a> Nonetheless, isomers ranging from 326 to 374 Da have been reported in SRMs for urban dust (1649a/b), diesel particulate (1650b and 2975), coal tar (1597a), and marine sediment (1941b), displaying qualitative differences between the sources in their large PAH compositions; <a href="javascript:void(0);">(18,20,22,23,27)</a> thus, large PAHs should offer forensic utility.</p><p>Recent advances in soft ionization within an atmospheric pressure chemical ionization source for gas chromatography–mass spectrometry (GC-APCI) are well-suited for the analysis of large PAHs. Two contributing factors for this are the tolerance for high carrier gas flow rates by GC-APCI and the sensitivity of charge exchange APCI for aromatic species. <a href="javascript:void(0);">(48)</a> Increasing the carrier gas flow rate reduces the elution temperature of analytes, making many higher mass analytes accessible using a conventional GC and column, although poor thermal stability may still pose challenges for some analytes. <a href="javascript:void(0);">(49)</a> Classic vacuum-source GC/MS systems using electron ionization (EI) restrict the upper carrier gas flow allowed from the GC, and therefore, cannot fully utilize high GC carrier gas flows. The sensitivity of GC-APCI for components of petroleum, such as PAHs, has been previously reported. <a href="javascript:void(0);">(48)</a> Furthermore, unlike LC-APCI, which commonly exhibits protonation of analytes in positive ion mode, <a href="javascript:void(0);">(50)</a> GC-APCI+ can operate with dry source conditions. These conditions result in nitrogen-mediated charge exchange ionization and ions of the form M+<strong><sup>•</sup></strong>, the same form of molecular ion created using EI. As a result, because the charge site location on an ion dictates which product ions will be stable, the same multiple reaction monitoring (MRM) transitions can be used for both GC-APCI MS/MS and EI MS/MS. <a href="javascript:void(0);">(51)</a></p><p>Herein, a GC-APCI method was developed for the detection of large PAHs. Due to the limited availability of pure standards, previously characterized NIST SRMs were used in method development as sources of large PAHs. The values of the method and detection of large PAHs were demonstrated by analyzing environmental plastic samples collected after the M/V <i>X-Press Pearl</i> ship fire and plastic spill.</p><h4><strong>Materials and Methods</strong></h4><h5><strong>GC-APCI</strong></h5><p>The solvent extracts and dilutions were analyzed by GC-APCI on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/554"><strong>Xevo TQ Absolute</strong></a> (Waters Corporation) tandem quadrupole mass spectrometer (TQ-MS/MS). The atmospheric pressure gas chromatography (APGC) ionization source was operated in charge transfer mode using dry N<sub>2</sub> as the reagent gas. Dry N<sub>2</sub> reagent gas creates molecular ions of the form M+<strong><sup>•</sup></strong> with no significant adduct formation for large PAHs. The high reagent gas flow rate of 350 mL/min into the ionization chamber, combined with low energies applied to the transfer optics from the atmospheric pressure region to the first quadrupole, leads to a high molecular ion survival rate. Furthermore, the use of MS/MS with quadrupoles operating at unit mass resolution prevents potential effects on response caused by adduct formation. Acquisitions were performed in positive ion, MRM mode. Two MRM transitions were used for each of the 16 precursor masses, ranging from 314 to 424 Da (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acsomega.5c11703/suppl_file/ao5c11703_si_001.pdf">Table S1</a>). No optimization of source conditions specific to the analysis of large PAHs was required. The list of 16 specific precursor masses targeted in this range was developed using multiple sources reporting large PAHs in NIST SRMs and samples from deep-sea hydrothermal vents. <a href="javascript:void(0);">(18,29,30)</a> The two transitions represented constant neutral losses of 2 and 4 Da at high collision energies of 60–100 eV, using N<sub>2</sub> as the collision gas.</p><p>For 1,3,5-TPB analysis, the APGC ionization source was operated in charge transfer mode using N<sub>2</sub> as the collision gas. Acquisitions were performed in positive ion, MRM mode, yielding six MRM transitions for analysis (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acsomega.5c11703/suppl_file/ao5c11703_si_001.pdf">Table S2</a>). Similar to the large PAHs, this analyte also shows neutral losses of 2 and 4 Da, reflecting behavior like that of the more condensed structures.</p><p>The <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=10&amp;search=8890+GC"><strong>8890 GC (Agilent Technologies)</strong></a> was configured with N<sub>2</sub> as the carrier gas and an <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=7&amp;search=Rxi-5HT"><strong>Rxi-5HT column (Restek)</strong></a> of 15 m in length, 0.25 mm inner diameter, and 0.10 μm film thickness. The split/splitless (SSL) injection port was operated at a 10:1 split ratio with a temperature of 380 °C. A 1 μL injection volume was used for all analyses. The temperature program was 30.8 min; it started by holding at 40 °C for 0.5 min, then ramped to 160 °C at 14 °C/min, followed by a ramp to 395 °C at 22 °C/min and a hold at the temperature for 11 min. The flow rate of the N<sub>2</sub> carrier gas was initially 0.60 mL/min, then ramped at 0.015 mL/min<sup>2</sup> to 0.90 mL/min, followed by a ramp at 0.150 mL/min<sup>2</sup> to 3.0 mL/min. To adapt the system for high-temperature work, the SSL was configured with a high-temperature septum (400 °C maximum), a 100% graphite liner O-ring, and a straight 4 mm inner diameter, wool-packed liner (450 °C maximum). Additional instrument and method details are included in the <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acsomega.5c11703/suppl_file/ao5c11703_si_001.pdf">Supporting Information</a>.</p><h4><strong>Results</strong></h4><h5><strong>Analysis of Large PAHs in Pyroplastic Samples by GC-APCI</strong></h5><p>Macroplastic and microplastic debris from the M/V <i>X-Press Pearl</i> ship fire and plastic spill were investigated for the presence and distribution of large PAHs. The field samples included white, unburnt polyethylene nurdles and two types of pyroplastic, termed burnt plastic and combustion remnant (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c11703#fig1">Figure 1</a>), both of which were polyethylene. <a href="javascript:void(0);">(43)</a> The types of pyroplastics were previously operationally defined based on the size and shape of the pieces. <a href="javascript:void(0);">(43)</a></p><p>Investigation of the 302 Da isomers revealed profiles similar to those of the SRMs; however, unique differences were observed (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acsomega.5c11703/suppl_file/ao5c11703_si_001.pdf">Figure S2</a>). Eighteen to twenty-one peaks were detected across the plastic samples. Compared with the SRMs, the pyroplastics showed decreases in the relative abundance of dibenzo[<i>a</i>,<i>e</i>]fluoranthene and increases in dibenzo[<i>a</i>,<i>h</i>]pyrene (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acsomega.5c11703/suppl_file/ao5c11703_si_001.pdf">Figure S2</a>). This trend was not the case for the white nurdle sample. Starting at a retention time of 16.80 min, two peaks were unique to the pyroplastic samples (not present in the SRMs and especially minor in the white nurdles). These peaks followed that for dibenzo[<i>a</i>,<i>h</i>]pyrene, which had previously been reported as the last detected 302 Da isomer compound in the SRMs, <a href="javascript:void(0);">(21)</a> supporting their novelty as marker compounds of pyroplastics.</p><p>The plastic samples displayed similarities and differences in the presence and distribution of large PAHs (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c11703#fig3">Figure 3</a>). The white nurdles, burnt plastic, and combustion remnant all contained high-intensity peaks at 20.50 min for 398 Da and 19.55 min for 374 Da. Peaks between 20.00 and 20.25 min of 398 Da and the 19.35 min peak of 374 Da were reduced in intensity for the pyroplastics compared to the white nurdles (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acsomega.5c11703/suppl_file/ao5c11703_si_001.pdf">Figure S3</a>). Conversely, the peaks between 18.50 and 18.75 min of 350 Da were more intense in the pyroplastics compared to the white nurdles.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_7_12321_12329_Figure_3_Comparison_of_the_resolved_isomers_for_398_374_and_350_Da_of_the_white_nurdles_burnt_plastic_and_combustion_remnant_pieces_ec390ebae3.jpg" alt="ACS Omega 2026, 11, 7, 12321–12329: Figure 3. Comparison of the resolved isomers for 398, 374, and 350 Da of the white nurdles, burnt plastic, and combustion remnant pieces collected from Pamunugama Beach, Sri Lanka, following the 2021 M/V X-Press Pearl ship fire and plastic spill." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_7_12321_12329_Figure_3_Comparison_of_the_resolved_isomers_for_398_374_and_350_Da_of_the_white_nurdles_burnt_plastic_and_combustion_remnant_pieces_ec390ebae3.jpg 232w," sizes="100vw" width="1600" height="1077"></p><p>The levels of all the targeted large PAHs were at least 2 orders of magnitude higher in the pyroplastics than in the white nurdles. These levels included the individual PAHs that were common to the pyroplastics. This trend mirrored that observed for smaller PAHs and the solvent-extractable content of the sample types. <a href="javascript:void(0);">(39,43,46)</a> There were also slight differences in the signatures of the large PAHs found in the pyroplastics. The 398 Da peak at 20.24 min, along with the 374 Da peak at 19.31 min and the 350 Da peak at 18.61 min, were more prominent in the combustion remnant compared to the burnt plastic (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acsomega.5c11703/suppl_file/ao5c11703_si_001.pdf">Figure S3</a>).</p><p>Similar to the differences in large PAH signatures between the SRMs, the plastic samples had different signatures. Such qualitative interpretation of extracted ion chromatograms is forensically accepted for distinguishing sources. <a href="javascript:void(0);">(3)</a> This feature can potentially be utilized to differentiate between plastic types (e.g., virgin, weathered, and partially combusted) as well as to identify sources of the large PAHs and the combustion temperature.</p><h4><strong>Conclusions</strong></h4><p>GC-APCI-MS/MS proved a useful technique for detecting large PAHs in diverse environmental matrices, including extracts from pyroplastic field samples. Despite a lack of widely available analytical standards for large PAHs, NIST SRMs provided ample material for method development for their detection. Nonetheless, with the increasing research on microplastics in the environment and their relevance to human health, an environmental SRM with certified values for large PAHs is needed, and pyroplastics may provide such a source of material. Large PAHs and 1,3,5-TPB were detected in pyroplastic field samples and hold promise as chemical markers for pyroplastics, complementing other means for their detection in environmental samples (e.g., appearance and physical properties). Due to the accessibility of GC-APCI, the detection of large PAHs can be integrated into workflows for evaluating environmental samples, including those for microplastics.</p></div>

Gas Chromatography-Atmospheric Pressure Chemical Ionization (GC-APCI) Expands the Analytical Window for Detection of Large PAHs (≥24 Ringed-Carbons) in Pyroplastics and Other Environmental Matrices

<p><strong>In the week of May 11, the following webinars await you in the field of gas / solid phase and especially Raman, FTIR, GC and GC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Uncovering Microplastics: Identification, Quantification, and Exposure Assessment</strong></h5><ul><li>Tu, 12.5.2026 08:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/6136">Registration</a></li></ul><p>In this webinar, we will explore complementary analytical solutions for comprehensive microplastics characterization.</p><h5><strong>2. AZoM: From Lab to Line: Leveraging GC and Process MS for Petrochemical Gas Analysis</strong></h5><ul><li>We, 13.5.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/6171">Registration</a></li></ul><p>Explore how GC and process MS work together in petrochemical workflows, combining detailed lab analysis with real-time gas monitoring for faster decisions.</p><h5><strong>3. Milestone: Modern Sample Prep for Organic Contaminants: PAHs, PFAS, MOSH/MOAH in Food</strong></h5><ul><li>We, 13.5.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/5822">Registration</a></li></ul><p>Learn how microwave-assisted extraction streamlines food analysis of PAHs, PFAS, and MOSH/MOAH, improving recoveries, reducing solvents, and meeting regulatory demands.</p><h5><strong>4. Thermo Fisher Scientific: End-to-end automation in GC/GC-MS: Trends, tools, and practical implementation</strong></h5><ul><li>We, 13.5.2026 18:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/6186">Registration</a></li></ul><p>In this GC/GC-MS power hour session, we’ll explore how automation is transforming laboratories by helping teams do more with less time, fewer resources, and greater confidence.</p><h5><strong>5. GERSTEL: Automating Food Safety Workflows for the Analysis of Edible Oils and Fats</strong></h5><ul><li>We, 13.5.2026 20:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/6168">Registration</a></li></ul><p>Learn how automated workflows improve data quality in edible oil testing, including FAMEs, MOSH/MOAH, and 3-MCPD analysis with real-world insights.</p>

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Thermo Scientific Trace™ TR-V1 GC Columns

Thermo Scientific™ TRACE TR-V1 GC Columns are mid-polarity, thick film columns.

<p><strong>Thermo Scientific™ TRACE TR-V1 GC Columns are mid-polarity, thick film columns.</strong></p><ul><li>Mid-polarity phase, 6% Cyanopropylphenyl Polysiloxane</li><li>Thick films for the analysis of volatile analytes</li><li>Low-bleed suitable for MS detection</li></ul><h4><strong>Recommended For:</strong></h4><ul><li>Volatile Organics</li><li>Alcohols</li><li>EPA 502.2, 608 and 624</li></ul><h4><strong>Similar To:</strong></h4><ul><li>DB-624</li><li>BPX Volatiles</li><li>Rtx Volatiles</li><li>VOCOL 56</li><li>OV-624</li><li>AT-624</li><li>HP-VOC</li><li>CP-Select 624CB</li><li>007-624</li><li>ZM-624</li></ul>

Thermo Scientific Trace™ TR-WAX GC Columns

Analyze a wide range of polar compounds with the Thermo Scientific™ TRACE™ TR-Wax GC Column.

<p><strong>Analyze a wide range of polar compounds with the Thermo Scientific™ TRACE™ TR-Wax GC Column. A polyethylene-glycol-based phase ideal for the analysis of all polar compounds, it is highly cross-linked and fully deactivated to provide excellent peak shape even with short chain alcohols.</strong></p><h4><strong>Engineered for Superior Performance</strong></h4><ul><li>Polar phase, polyethylene glycol</li><li>Highly cross-linked and fully deactivated</li><li>Solvent washable</li></ul><h4><strong>Excellent Quality and Reproducibility:</strong></h4><ul><li>Low-bleed even at elevated temperatures</li><li>Outstanding robustness for difficult separations</li><li>Reliable and reproducible performance</li></ul><h4><strong>Applications:</strong></h4><ul><li>Esters</li><li>Alcohols</li><li>Ketones</li><li>Glycols</li><li>Aromatic isomers</li></ul><h4><strong>Recommended Use:</strong></h4><ul><li>Aldehydes</li><li>Essential Oils</li></ul><h4><strong>Alternative Uses:</strong></h4><ul><li>Acids</li><li>Alcohols</li><li>Alcohols in beverages</li><li>Alditol acetates (sugars)</li><li>Amines–Aliphatic</li><li>Amines–Aromatic</li><li>Herbicides</li><li>Ketones</li><li>Nitroaromatics</li><li>Phenols</li><li>Volatiles</li></ul><h4><strong>Similar To:</strong></h4><ul><li>DB-Wax</li><li>BP20</li><li>Rtx™-Wax</li><li>Stabilwax™</li><li>HP20M</li><li>HP-Wax</li><li>HP-INNOWax</li><li>SUPELCOWAX™ 10</li><li>AT™-Wax</li><li>Nukol™</li><li>CP Wax52CB</li><li>SUPEROX II</li><li>Carbowax™</li><li>PE-WAX</li><li>ZBWAX</li></ul>

Thermo Scientific Trace™ TR-WaxMS GC Columns

For high-polarity separations as part of GC/MS analysis, choose the low-bleed, high polarity Thermo Scientific™TRACE™ TR-WaxMS GC Column. 

<p><strong>For high-polarity separations as part of GC/MS analysis, choose the low-bleed, high polarity Thermo Scientific™TRACE™ TR-WaxMS GC Column. The TRACE TR-WaxMS column is manufactured using a proprietary bonding technique that minimizes bleed and extends the maximum operating temperature (260 to 280 ̊C).</strong></p><h4><strong>Engineered for Superior Performance:</strong></h4><ul><li>Polar phase, polyethylene glycol</li><li>Proprietary bonding method for expanded operating temperatures</li><li>Extremely low-bleed for improved sensitivity and library matches</li><li>High stability with oxygen and water</li></ul><h4><strong>Excellent Quality and Reproducibility:</strong></h4><ul><li>Low-bleed even at elevated temperatures</li><li>Outstanding robustness for difficult separations</li><li>Reliable and reproducible performance</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Trace_TR_Wax_MS_GC_Columns_676d6b9e2e.jpg" alt="Thermo Scientific: Trace™ TR-WaxMS GC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Trace_TR_Wax_MS_GC_Columns_676d6b9e2e.jpg 244w," sizes="100vw" width="650" height="416"></p><h4><strong>Applications:</strong></h4><ul><li>Aromatic hydrocarbons</li><li>Food additives</li><li>Essential oils</li><li>Alcohols</li><li>Esters</li><li>Aldehydes</li><li>Ketones</li></ul><h4><strong>Recommended Use:</strong></h4><ul><li>Aldehydes</li><li>Essential Oils</li></ul><h4><strong>Alternative Uses:</strong></h4><ul><li>Acids</li><li>Alcohols</li><li>Alcohols in beverages</li><li>Alditol acetates (sugars)</li><li>Amines&amp;endash;Aliphatic</li><li>Amines&amp;endash;Aromatic</li><li>Herbicides</li><li>Ketones</li><li>Nitroaromatics</li><li>Phenols</li><li>Volatiles</li></ul><h4><strong>Similar To:</strong></h4><ul><li>DB-Wax</li><li>Rtx™-Wax</li><li>Stabilwax™</li><li>HP20M</li><li>BP20</li><li>HP-Wax</li><li>HP-INNOWax</li><li>SUPELCOWAX™ 10</li><li>AT™-Wax</li><li>Nukol™</li><li>CP Wax 52CB</li><li>ZB-Wax</li></ul>

Thermo Scientific Trace™ TR-FAME GC Columns

Choose Thermo Scientific™ TRACE™ TR-FAME GC Columns for separation of cis/trans isomers and fatty acid methyl esters (FAMEs) with various degrees of unsaturation.

<p><strong>Choose Thermo Scientific™ TRACE™ TR-FAME GC Columns for separation of cis/trans isomers and fatty acid methyl esters (FAMEs) with various degrees of unsaturation. The TRACE TR-FAME column is a cyanopropylphenyl-based phase specifically designed for the separation of FAMEs. It is also suitable for any application that requires a high-polarity column for use with MS detection.</strong></p><h4><strong>Excellent Quality and Reproducibility</strong></h4><ul><li>Low bleed for MS, even at elevated temperatures</li><li>Outstanding robustness for difficult separations</li><li>Reliable and reproducible performance</li><li>Polar phase, 70% cyanopropyl polysilphenylene-siloxane</li><li>Higher operating temperature than other columns</li></ul><h4><strong>Applications:</strong></h4><ul><li>FAMEs</li><li>FAMEs cis/trans isomers</li></ul><h4><strong>Similar To:</strong></h4><ul><li>DB-23</li><li>BPX70</li><li>Rtx™-2330</li><li>SP™-2330</li><li>CP-Sil 88</li><li>SP™-2380</li><li>HP-23</li><li>VF-23ms</li><li>007-23</li><li>AT™-Silar</li><li>PE-23</li></ul>

Thermo Scientific Trace™ TR-FFAP GC Columns

Low-bleed even at elevated temperatures. Chose the Thermo Scientific™ TRACE™ TR-FFAP GC Columns for outstanding robustness in difficult-to-analyze separations.

<p><strong>Low-bleed even at elevated temperatures. Chose the Thermo Scientific™ TRACE™ TR-FFAP GC Columns for outstanding robustness in difficult-to-analyze separations.</strong></p><h4><strong>Engineered Specifically for FFAP Analysis:</strong></h4><ul><li>Polar phase, TPA modified polyethylene glycol</li><li>Bonded FFAP phase</li><li>Quality tested for acidic compound analysis</li><li>Equivalent to USP G25 and G35</li><li>Excellent Quality and Reproducibility</li><li>Low-bleed even at elevated temperatures</li><li>Outstanding robustness for difficult-to-analyze separations</li><li>Reliable and reproducible performance</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Trace_TR_FFAP_GC_Columns_56b870dff4.jpg" alt="Thermo Scientific: Trace™ TR-FFAP GC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Trace_TR_FFAP_GC_Columns_56b870dff4.jpg 244w," sizes="100vw" width="650" height="416"></p><h4><strong>Recommended Use:</strong></h4><ul><li>Acids</li><li>FFAP analysis</li><li>Alcohols</li></ul>

Thermo Scientific Trace™ TR-BioDiesel GC Columns

Determine methanol, fatty acid methyl esters (FAMEs) and glycerides in biodiesel analysis with high performance and reproducibility using Thermo Scientific™ TRACE™ TR-BioDiesel GC Columns.

<p><strong>Determine methanol, fatty acid methyl esters (FAMEs) and glycerides in biodiesel analysis with high performance and reproducibility using Thermo Scientific™ TRACE™ TR-BioDiesel GC Columns.</strong>&nbsp;</p><p>Biodiesel is an important component in the development of carbon neutral fuels and specific GC columns within the TRACE range deliver robust performance to meet and exceed the methodology demands for this application area.</p><h4><strong>Columns Specifically Designed to Meet Your Biodiesel Analysis Needs:</strong></h4><ul><li><strong>TRACE TR-BioDiesel (M)</strong>; for determination of residual methanol content in biodiesel according to EN Method 14110</li><li><strong>TRACE TR-BioDiesel (G)</strong>; for the analysis of total glyceride content according to EN Method 14105</li><li><strong>TRACE TR-BioDiesel (F)</strong>; for the analysis of FAME content in biodiesel according to EN Method 14103</li><li><strong>TRACE TR-BioDiesel (G) ASTM</strong>; for the analysis of total glyceride content according to ASTM method D-6584</li></ul><h4><strong>Excellent Quality and Reproducibility:</strong></h4><ul><li>Low-bleed even at elevated temperatures</li><li>Outstanding robustness for difficult separations</li><li>Reliable and reproducible performance</li></ul>

Thermo Scientific Trace™ GC Columns for Pesticides

Identify organophosphate and organochlorine pesticides with high performance, sensitivity, and reproducibility using the Thermo Scientific™ TRACE™ GC Columns for Pesticides. 

<p><strong>Identify organophosphate and organochlorine pesticides with high performance, sensitivity, and reproducibility using the Thermo Scientific™ TRACE™ GC Columns for Pesticides. These columns are specifically designed and tested to meet the most stringent requirements for the analysis of pesticides and herbicides.</strong></p><h4><strong>Excellent Quality and Reproducibility:</strong></h4><ul><li>Low bleed decreases MS contamination</li><li>Innovative design facilitates use at higher temperatures</li><li>Column inertness results in minimal peak tailing and decreased breakdown of sensitive samples</li></ul><h4><strong>Alternative Uses:</strong></h4><ul><li>Chlorinated aromatics</li><li>Plant sterols</li><li>Pyrethroids</li></ul>

Thermo Scientific Trace™ GC Columns for Specific EPA Methods

Perform specific EPA methodologies with confidence and unsurpassed performance using the Thermo Scientific™ TRACE™ GC Capillary Columns for Specific EPA Methods. 

<p><strong>Perform specific EPA methodologies with confidence and unsurpassed performance using the Thermo Scientific™ TRACE™ GC Capillary Columns for Specific EPA Methods. These low-bleed and temperature-stable columns have been designed, manufactured and tested to provide consistent, reliable results from run-to-run and batch-to-batch.</strong></p><h4><strong>Columns Tailored Specifically for U.S. EPA Test Methods:</strong></h4><ul><li><strong>TRACE TR-524 and TRACE TR-525:</strong> U.S. EPA Drinking Water Test Methods 524 or 525</li><li><strong>TRACE TR-527:</strong> U.S. EPA Drinking Water Test Method 527, featuring the robust, low-bleed performance required for analysis of pesticides and flame retardants</li><li><strong>TRACE TR-8270: </strong>U.S. EPA Solid Waste Test Method 8270</li><li><strong>TRACE TR-8095: </strong>U.S. EPA Method 8095 for Explosives Testing, featuring high maximum temperature and low surface activity</li></ul><h4><strong>Excellent Quality and Reproducibility:</strong></h4><ul><li>Low bleed, even at elevated temperatures</li><li>Outstanding robustness for difficult separations</li><li>Reliable and reproducible performance</li></ul><h4><strong>Applications:</strong></h4><ul><li>Volatile organic compounds (VOCs)</li><li>Pesticides</li><li>Brominated flame retardants</li><li>Explosives</li></ul>

Thermo Scientific Trace™ GC Columns for Dioxin and PCB Analysis

Analyze dioxins and polychlorinated biphenyls (PCBs) with custom-tailored Thermo Scientific™ TRACE™ GC Columns for Dioxin and PCB Analysis

<p><strong>Analyze dioxins and polychlorinated biphenyls (PCBs) with custom-tailored Thermo Scientific™ TRACE™ GC Columns for Dioxin and PCB Analysis for unsurpassed performance and reproducibility.&nbsp;</strong></p><p>Optimized to meet the requirements of high-resolution GC/MS methods, these columns deliver optimum performance with exceptional stability and very low bleed. These low-polarity columns provide wide coverage of the 17 cogeners with the highest toxicological significance (2,3,7,8-chlorine substituted congeners).</p><h4><strong>Columns Tailored Specifically for Dioxins and PCBs:</strong></h4><ul><li><strong>TRACE TR-Dioxin 5MS: </strong>dioxin and furan testing</li><li><strong>TRACE TR-PCB 8MS: </strong>high-resolution GC/MS analysis of PCBs</li></ul><h4><strong>Excellent Quality and Reproducibility</strong></h4><ul><li>Low bleed, even at elevated temperatures</li><li>Outstanding robustness for difficult separations</li><li>Reliable and reproducible performance</li></ul><h4><strong>Applications:</strong></h4><ul><li>Furan (PCDF) Analysis</li><li>GC/MS for Furan (PCDF) Analysis</li><li>GC/MS for PCB Congener Analysis</li></ul>

Phenomenex Zebron ZB-1XT SimDist GC Columns

Zebron ZB-1XT SimDist columns deliver outstanding reproducibility and up to 70% higher efficiency thanks to Glass Infusion™ technology—ensuring sharper peaks, accurate high-temperature SimDist, and long column lifetime.

<h4><strong>Consistent, Reliable Results for Simulated Distillation</strong></h4><ul><li>Glass Infusion™ technology for higher efficiency and greater column-to-column reproducibility</li><li>Individual QC testing for every column</li><li>Up to 70 % higher efficiency than other columns</li><li>Increased accuracy for high temperature simulated distillation</li></ul><p>Want a flexible fused-silica alternative for SimDist testing? See <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/1563"><strong>ZB-1HT-Inferno</strong></a></p><h5><strong>Recommended Use</strong></h5><ul><li>ASTM Methods (D2887, D2887X, D3710, D6352, D7169), Crude Oil, Gasoline Fractions, Petroleum Distillates, Petroleum Fractions, Simulated Distillation, Vacuum Distillates</li></ul><h5><strong>Upgrade to Zebron from any 100 % dimethylpolysiloxane phase:</strong></h5><p><strong>Agilent®&nbsp;&nbsp;</strong>&nbsp;&nbsp;</p><ul><li>DB®-1</li><li>DB-HT SimDis</li><li>DB-PS1</li><li>DB-PS2887</li><li>CP-SimDist</li><li>CP-SimDist UltiMetal</li></ul><p><strong>Restek®</strong></p><ul><li>Rtx®-1</li><li>Rxi®-1HT</li><li>MXT®-1HT SimDist</li></ul><p><strong>SGE®</strong></p><ul><li>BP1</li><li>BPX1-SimD</li></ul><h3><strong>Sharper Peak Shape and Better Efficiency through Uniform Glass Infusion™ Coating Technology</strong></h3><ul><li><strong>Sharper Peaks and Higher Efficiency:</strong><ul><li>Glass Infusion Technology Provides &nbsp; &nbsp;&nbsp;</li><li>Uniform Coating and Minimizes</li><li>Unwanted Activity.</li></ul></li><li><strong>Consistency and Reliability:</strong><ul><li>Zebron Columns are INDIVIDUALLY</li><li>Tested for Better Reproducibility and</li><li>Overall Performance.</li></ul></li><li><strong>Higher Efficiency:</strong><ul><li>Up to 70 % Higher Column Efficiency.</li></ul></li><li><strong>Long Lifetime and Better Results:</strong><ul><li>Improved Resolution of C50/C52 &nbsp; &nbsp;&nbsp;</li><li>Hour after Hour.</li></ul></li><li><strong>Guaranteed:</strong><ul><li>100 % Risk-Free</li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_1_XT_Sim_Dist_GC_Columns_dd3e7f0c32.jpg" alt="Phenomenex: Zebron ZB-1XT SimDist GC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_1_XT_Sim_Dist_GC_Columns_dd3e7f0c32.jpg 146w," sizes="100vw" width="677" height="722"></p><h3><strong>Improved Recovery and Peak Shape</strong></h3><h4><strong>The Benefits of Glass Infusion™ Technology</strong></h4><p>The Zebron ZB-1XT SimDist metal columns employ a new proprietary bonding called Glass Infusion Technology. The Glass Infusion technology starts by depositing a very uniform layer of glass that completely covers the internal metal surfaces of the column. The result is a uniform coating throughout the column.</p><h5><strong>Glass Enclosed</strong></h5><ul><li>Uniform Glass Infusion ensures adequate covering of exposed metal to promote better recovery and peak shape.</li><li>Each Zebron ZB-1XT is individually tested to confirm and ensure excellent reproducibility.</li><li>The uniform glass coating provides a consistent foundation for stationary phase bonding, thus promoting better over-all efficiency and resolution.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Glass_Enclosed_b434ad6540.jpg" alt="Phenomenex: Glass Enclosed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Glass_Enclosed_b434ad6540.jpg 245w," sizes="100vw" width="709" height="232"></p><h5><strong>Exposed Metal</strong></h5><ul><li>Non-glass infused with exposed metal can cause tailing peaks and sample adsorption.</li><li>Some manufacturers batch test their columns, leading to poor column-to-column reproducibility.</li><li>These exposed surfaces result in abnormal bonding of the stationary phase which affects chromatographic performance, decreases efficiencies, and inconsistent performance between columns.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Exposed_Metal_00e4466d96.jpg" alt="Phenomenex: Exposed Metal" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Exposed_Metal_00e4466d96.jpg 245w," sizes="100vw" width="706" height="237"></p>

Products focused on GC columns - page 1

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