<p><strong>🎤Alex Brody</strong></p><p><strong>In this episode of Concentrating on Chromatography, we sit down with Alex Brody, an organic chemist at Aqua America, to discuss the rigorous analytical methods required for regulated drinking water testing.</strong></p><p>Alex walks us through the multi-step PFAS detection process using EPA Method 537.1, including extraction, nitrogen blowdown concentration, and LC-MS analysis. He explains why sample preparation and quality control are critical for achieving trace-level detection—and why these methods can't be rushed or simplified, even with new technologies available.</p><p>We also explore taste and odor investigations, disinfection byproducts, and volatile organic compounds, plus the surprising reality of why regulated labs move slowly when adopting new instrumentation. You'll learn about the quality control checkpoints, peer review processes, and the misconceptions surrounding analytical turnaround times in compliance labs.</p><h5><strong>Topics Covered:</strong></h5><ul><li>Role of organic chemistry in regulated drinking water labs</li><li>EPA compliance requirements and regulatory bodies (EPA, PA-DEP)</li><li>PFAS detection using Method 537.1 and LC-MS</li><li>The critical importance of nitrogen blowdown sample concentration</li><li>Taste and odor analysis: MIB, Geosmin, and unknown compound identification</li><li>Why regulated methods evolve slowly (validation timelines, approval processes)</li><li>Quality control procedures: calibration checks, matrix spikes, surrogate standards, internal standards</li><li>Automated sample preparation and lab efficiency</li><li>Future PFAS regulations and Method 533 expansion</li></ul><h5><strong>Ideal For:</strong></h5><ul><li>Analytical chemists and environmental lab professionals</li><li>Water utilities and compliance officers</li><li>Chromatography practitioners interested in regulated workflows</li><li>Anyone curious about how drinking water safety is ensured</li></ul><h2><strong>Video Transcription</strong></h2><h3><strong>Ensuring Safe Drinking Water Through Advanced Analytical Chemistry</strong></h3><h4><strong>Insights from a Regulated Water Testing Laboratory</strong></h4><p>Analytical laboratories responsible for regulated drinking water testing operate under strict compliance frameworks to ensure the delivery of safe and reliable water supplies. In this interview, Alex, an organic chemist at Aqua America, outlines the scope of analytical work conducted in a modern water quality laboratory. The team primarily focuses on the determination of taste- and odor-causing compounds, disinfection byproducts, volatile organic compounds (VOCs), and per- and polyfluoroalkyl substances (PFAS). These analyses rely heavily on <strong>GC-MS and LC-MS instrumentation</strong>, supported by rigorous maintenance programs to ensure continuous operational performance and data reliability.</p><h4><strong>Regulatory Compliance and Quality Control in Drinking Water Analysis</strong></h4><p>Operating as a regulated drinking water laboratory requires adherence to stringent requirements established by agencies such as the <strong>U.S. Environmental Protection Agency (EPA)</strong> and state-level regulatory bodies. Compliance is driven by extensive quality control protocols integrated into every analytical batch. Typical workflows include calibration verification, matrix spike recovery assessments, internal standard monitoring, and surrogate performance checks. Notably, analytical batches are often limited in sample number to accommodate a substantial proportion of quality control samples, reflecting the critical importance of data defensibility in regulatory reporting.</p><p>One common misconception is the perceived speed at which results can be delivered. In reality, comprehensive sample preparation, including extraction procedures and validation steps, can extend turnaround times to a full working day or longer. Additionally, validated regulatory workflows impose limitations on the rapid adoption of new analytical technologies, as method modifications must undergo formal evaluation and approval before implementation.</p><h4><strong>Analytical Strategies for Taste and Odor Investigations</strong></h4><p>Taste and odor events represent a significant operational challenge for water utilities. Standard targeted methods are routinely applied for compounds such as <strong>2-methylisoborneol (MIB)</strong> and <strong>geosmin</strong>, which are commonly associated with earthy or musty sensory characteristics. When targeted analysis does not reveal the source, laboratories employ <strong>broad-scan GC-MS screening</strong> combined with spectral library interpretation to identify potential causative compounds. In some cases, sensory evaluation panels complement instrumental analysis, providing preliminary insight into potential treatment-related or environmental influences affecting water quality.</p><h4><strong>PFAS Monitoring: Methodological Complexity and Trace-Level Sensitivity</strong></h4><p>PFAS determination represents a rapidly evolving priority in drinking water monitoring. The laboratory currently applies <strong>EPA Method 537.1</strong>, with efforts underway to expand capabilities through certification for <strong>Method 533</strong>, thereby broadening analyte coverage. Analytical workflows typically involve solid-phase extraction followed by solvent evaporation using nitrogen-assisted blow-down techniques to achieve the trace-level sensitivity required for regulatory compliance. This concentration step is essential because PFAS compounds remain non-volatile under controlled evaporation conditions, enabling enhanced detection performance upon reconstitution and LC-MS analysis.</p><h4><strong>Future Directions in Drinking Water Analytics</strong></h4><p>Looking ahead, the primary technological driver in regulated water analysis is the demand for <strong>increasingly lower detection limits</strong> and expanded compound coverage. While regulatory frameworks can slow methodological innovation, laboratories continue to pursue efficiency improvements, such as automated sample preparation systems and optimized analytical workflows. These advances support the growing need to process high sample volumes while maintaining the stringent data quality standards required for public health protection.</p><p>Ultimately, the work performed in regulated drinking water laboratories represents a critical intersection of analytical chemistry, environmental monitoring, and public safety. Continuous methodological refinement, coupled with advances in mass spectrometry and sample preparation technologies, will play a central role in addressing emerging contaminants and ensuring long-term water quality assurance.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Regulated Drinking Water Testing: PFAS Detection, Sample Prep & Real Lab Insights

<p><strong>This study investigates the chemical transformations of Eugenia uniflora essential oil under thermal conditions simulating gas chromatography analysis. GC–MS and NMR revealed that sesquiterpenes of the germacrene type, such as furanodiene, undergo sigmatropic rearrangement to form elemene-type compounds, including curzerene.</strong></p><p>Thermal treatment confirmed the formation of rearrangement products, indicating that bioactive properties historically attributed to curzerene may instead originate from furanodiene. The findings highlight the importance of combining complementary analytical techniques, such as NMR, to avoid misinterpretation of essential oil composition and bioactivity markers.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966"><strong>Is Curzerene Responsible for the Bioactive Properties of Eugenia uniflora? A Possible Misinterpretation of Bioactive Markers</strong></a></h4><p>Vinicius Monteiro Schaffka, Raphaela Pereira Guaringue, Larissa Kozan, André Luis Kerek, Cássia Gonçalves Magalhães, Andersson Barison, and Barbara Celânia Fiorin*</p><p>ACS Omega 2025, 10, 48, 59425–59434</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acsomega.5c08966">https://doi.org/10.1021/acsomega.5c08966</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><div><p>Essential oils are a matrix of volatile and complex compounds characterized by strong odors and significant biological activity. In plants, they play a crucial role in defense against bacteria, viruses, and predators. <a href="javascript:void(0);">(1)</a> These biological properties support the extensive use of essential oils in the food, cosmetics, and pharmaceutical industries. <a href="javascript:void(0);">(1,2)</a> <i>Eugenia uniflora</i> L., commonly known as pitangueira, is a native Brazilian tree widely distributed across South America and is well-known for its bioactive essential oil. <a href="javascript:void(0);">(3)</a> Beyond the consumption of its fruits in natura or functional beverages, its leaves are used in infusions, decoctions, and tinctures to treat a variety of diseases, including diarrhea, stomach pain, worm infestations, fever, flu, hyperglycemia, hyperlipidemia, and hypertension. <a href="javascript:void(0);">(4,5)</a> Studies have shown that the composition of secondary metabolites in <i>E. uniflora</i> can vary depending on factors such as fruit color, ripening stage, <a href="javascript:void(0);">(6)</a> and seasonal influences. <a href="javascript:void(0);">(4,7−10)</a></p><p>The essential oil of <i>E. uniflora</i> is predominantly composed of sesquiterpenes, mainly germacrene-type, such as curzerene, germacrene B, germacrone, germacrene D, selina-1,3,7(11)-trien-8-one, and its oxide. Additionally, Elemene-type terpenes are also reported, including furanodiene, β-Elemene, and β-Elemenone. <a href="javascript:void(0);">(11−13)</a></p><p>However, those sesquiterpenes are well-documented for their thermal instability. When heat is applied, germacrene-type sesquiterpenes undergo a [3,3]-sigmatropic rearrangement, known as Cope rearrangement, converting into Elemene-type terpenes as presented in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966#fig1">Figure 1</a>. <a href="javascript:void(0);">(14−16)</a> This reaction is widely utilized as a synthetic pathway to produce Elemenes from germacrene precursors. <a href="javascript:void(0);">(17,18)</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_48_59425_59434_Figure_1_3_3_Sigmatropic_rearrangement_of_Germacrene_type_into_Elemene_type_250708e73c.jpg" alt="ACS Omega 2025, 10, 48, 59425–59434: Figure 1. [3,3]-Sigmatropic rearrangement of Germacrene-type into Elemene-type." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_48_59425_59434_Figure_1_3_3_Sigmatropic_rearrangement_of_Germacrene_type_into_Elemene_type_250708e73c.jpg 245w," sizes="100vw" width="3000" height="984"></p><p>Gas chromatography (GC) is the main technique for characterizing essential oils, often coupled with mass spectrometry (GC-MS) and the retention index determination is used in the identification of the compounds through comparison with databases. <a href="javascript:void(0);">(21)</a> During GC analysis, essential oils are subjected to multiple heating stages. Initially, the sample is exposed to a high inlet temperature, typically around 240 °C, to ensure complete volatilization. It then enters the oven, where the temperature follows a programmed ramp starting at 60 °C, increasing at 3 °C/min, and reaching up to 240 °C. Finally, the sample encounters the elevated temperatures of the mass spectrometry (MS) detector’s source (250 °C) and interface (280 °C). Even in isocratic methods, where the oven temperature remains constant throughout the analysis, the inlet, source, and interface temperatures are maintained at high levels. Although the sample passes through these stages briefly, the elevated temperatures can induce Cope rearrangements in the essential oil, leading to possible changes in its composition. <a href="javascript:void(0);">(22,23)</a></p><p>The accurate characterization of the chemical composition of essential oils is crucial for any study employing them as a biological or analytical matrix, as it supports the interpretation of bioactivity, chemotaxonomic classification, and quality control of samples with economic value added. There are many studies attributing relevant biological activities, classifying specimens, and identifying phenotypic and genetic variations based on the chemical composition of the essential oil of <i>E. uniflora</i> L., which is usually characterized by GC-MS. <a href="javascript:void(0);">(8,9,11,24)</a> As an example of the study carried out by de Jesus, found acute anti-inflammatory effect of a rich in curzerene essential oil. <a href="javascript:void(0);">(5)</a> Similarly, Pascoal, reported distinct essential oil composition patterns among <i>E. uniflora</i> varieties─yellow, red, and purple fruits─primarily driven by variations in β-Elemene and germacrene D content. <a href="javascript:void(0);">(7)</a> These findings highlight metabolic changes during fruit ripening and underscore the influence of genetic diversity among <i>E. uniflora</i> varieties. Such exhibitions are unsatisfactory, because they sustain all their evidence in the characterization of their essential oil only by GC-MS analysis, without considering the Cope rearrangements, a fact that may change the outcome of their research. In this context Nuclear Magnetic Resonance (NMR) spectroscopy is a good alternative for essential oil analysis, since is a robust not destructive technique and able to detect and quantify simultaneously a wide range of metabolites that exceed the concentration of about 5–10 μM. <a href="javascript:void(0);">(25)</a></p><p>In order to highlight analytical concerns about the uses of only GC-MS data to access chemical relevance of <i>E. uniflora</i> essential oil studies, Nuclear Magnetic Resonance (NMR) spectroscopy was employed to evaluate the chemical composition of the essential oil of <i>Eugenia uniflora</i> L. before and after an induced thermal treatment, compared to GC-MS data. The findings provide insights into thermolabile compounds and highlight potential misidentifications of the bioactive properties associated with the sesquiterpenes of <i>E. uniflora</i> present in literature.</p><h4><strong>2. Results and Discussion</strong></h4><h5><strong>2.1. Characterization of the Essential Oil</strong></h5><p>Approximately 60 compounds, mostly sesquiterpenes (Germacrene B) and oxygenated sesquiterpenes (Germacrone), were visualized in the essential oil, with the 9 compounds having a relative area above 2%, as shown in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966#tbl1">Table 1</a>. The identified and most prevalent compounds align with those reported in the literature for <i>E. uniflora</i>. <a href="javascript:void(0);">(4,11,12)</a> It is possible to highlight the significant proportion of Germacrene-type sesquiterpenes compared to Elemene-type, except Curzerene, which was found in high concentration.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_48_59425_59434_Table_1_Main_Components_Identified_in_the_GC_MS_Analysis_of_the_Essential_Oil_from_Leaves_of_E_uniflora_9d59d96bef.jpg" alt="ACS Omega 2025, 10, 48, 59425–59434: Table 1. Main Components Identified in the GC–MS Analysis of the Essential Oil from Leaves of E. uniflora." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_48_59425_59434_Table_1_Main_Components_Identified_in_the_GC_MS_Analysis_of_the_Essential_Oil_from_Leaves_of_E_uniflora_9d59d96bef.jpg 218w," sizes="100vw" width="1076" height="771"></p><p>The variation in the composition of the essential oil from <i>E. uniflora</i> leaves was described in literature, <a href="javascript:void(0);">(9)</a> who classified the specimens into three groups: Group I, containing yellow, dark red, and purple fruits, which showed high concentrations of germacrene B, germacrone, and atractylone; Group II, consisting of light red fruit samples, with high concentrations of curzerene, germacrene D, and germacrene A; and Group III, containing orange-red fruits, with a high content of selina-1,3,7(11)-trien-8-one and oxyselina-1,3,7(11)-trien-8-one. According to this classification, this essential oil fits into Group II.</p><p>Chemical transformations of many components found in the <i>E. uniflora</i> leaves essential oil result from the heating conditions typically applied during GC–MS analysis, which occur through Cope Rearrangement. <a href="javascript:void(0);">(12,19)</a> Cope rearrangement is a stereospecific [3,3]-sigmatropic rearrangement that proceeds via the most stable chairlike transition state of 1,5-cyclodecadiene, from germacrene-type, to give 1,2-divinylcyclohexane, known as Elemene-type. <a href="javascript:void(0);">(14)</a> Some of the Cope rearrangements found in the <i>E. uniflora</i> essential oil are represented in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966#fig2">Figure 2</a>. <a href="javascript:void(0);">(11,22,23,26)</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_48_59425_59434_Figure_2_Cope_Rearrangements_of_sesquiterpenes_7fe9282d75.jpg" alt="ACS Omega 2025, 10, 48, 59425–59434: Figure 2. Cope Rearrangements of sesquiterpenes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_48_59425_59434_Figure_2_Cope_Rearrangements_of_sesquiterpenes_7fe9282d75.jpg 150w," sizes="100vw" width="1500" height="1566"></p><p>The same essential oil was analyzed using NMR spectroscopy to identify its components. Although NMR identification is considerably more labor-intensive than GC-MS analysis, it offers the advantage of being a cold and nondestructive technique. Since the sample does not undergo heating during the process, its stability is preserved throughout the analysis. The conformational differences observed in germacrene-type compounds further increase the spectrum’s complexity, leading to signal broadening in the <sup>1</sup>H NMR spectra. <a href="javascript:void(0);">(27)</a></p><p>A substantial number of signals were observed in the <sup>1</sup>H spectrum, with the majority concentrated between 0.5 and 3.0 ppm, a region characteristic of terpene compounds. The region between 4.5 and 6.0 ppm also exhibited numerous signals attributed to vinylic protons, observed in sesquiterpenes (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966#fig3">Figure 3</a>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_48_59425_59434_Figure_3_1_H_NMR_400_13_M_Hz_CD_Cl3_spectra_of_the_raw_essential_oil_a46e04574e.jpg" alt="ACS Omega 2025, 10, 48, 59425–59434: Figure 3. 1H NMR (400.13 MHz, CDCl3) spectra of the raw essential oil." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_48_59425_59434_Figure_3_1_H_NMR_400_13_M_Hz_CD_Cl3_spectra_of_the_raw_essential_oil_a46e04574e.jpg 245w," sizes="100vw" width="4175" height="2376"></p><h4><strong>3. Conclusions</strong></h4><p>The investigation of the composition of <i>E. uniflora</i> essential oil by NMR and the thermal treatment performed proved that curzerene may come from furanodiene due to the transformations that occur in the matrix during gas chromatographic analysis. These findings have significant implications for the investigation into the chemical composition of <i>E. uniflora</i> essential oil and the evaluation of the biological properties of its sesquiterpenes. Since each technique has advantages and disadvantages, combining GC-MS analysis with a cold technique, such as NMR is important to guarantee the proper identification of the compound genuinely responsible for any property assigned to <i>E. uniflora</i> essential oil.</p><h4><strong>4. Materials and Methods</strong></h4><h5><strong>4.2. GC-MS Analysis</strong></h5><p>The gas chromatographic analyses of the essential oils (10 mg mL<sup>–1</sup>) were performed using a gas chromatography–mass spectrometry system (<a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=1&amp;search=QP2020"><strong>Shimadzu GCMS-QP2020 Gas Chromatograph</strong></a>). The analyses were conducted on an RTx-5MS capillary column (30 m × 0.25 mm internal diameter × 0.25 μm film thickness), and the analytical conditions were as follows: split ratio of 1/10, injector temperature at 250 °C, ion source at 250 °C, and interface at 280 °C. The temperature program of the oven was set at 60 °C for 5 min, followed by a temperature ramp of 3 °C/min until reaching the final temperature of 240 °C. The components were identified based on the relative retention index, calculated for each constituent by injecting a series of <i>n</i>-alkane standards (C8–C20) under the same sample conditions and comparing them with tabulated values as well as by comparing the obtained mass spectra with the mass spectra database and literature comparisons. <a href="javascript:void(0);">(21)</a></p><h5><strong>4.4. NMR Analysis</strong></h5><p>All NMR spectra were acquired at 298 K on a <strong>Bruker AVANCE III</strong> spectrometer operating at 9.4 T, observing <sup>1</sup>H at 400.13 MHz and <sup>13</sup>C at 100.13 MHz, equipped with a broadband probe. The essential oil was solubilized in CDCl<sub>3</sub> with 0.05% of tetramethylsilane (TMS) used as an internal reference. The <sup>1</sup>H NMR spectra were recorded utilizing 65k time-domain data points across a spectral width of 20.00 ppm. This setup provided a digital resolution of 0.24 Hz. The data were obtained using a single 30° excitation pulse, with a relaxation delay of 1.0 s, and an average of 128 scans. The <sup>13</sup>C NMR spectra was carried out using the <i>zgpg30</i> pulse sequence utilizing 64k time-domain data points across a spectral width of 238 ppm, with an acquisition time of 1.3631 s, providing a digital resolution of 0.7434 Hz and a relaxation delay of 2.0s and an average of 2k scans.</p><p>The HSQC and HMBC experiments were conducted using standard pulse sequences from the Bruker library, with modifications to the number of acquired points to improve resolution, 2048 in F2 and 512 in F1.</p></div>

Is Curzerene Responsible for the Bioactive Properties of Eugenia uniflora? A Possible Misinterpretation of Bioactive Markers

Quantitative Assessment of Chemical Modifications of Macromolecules through Counterion Measurement

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Thermo Scientific™ Exactive™ GC Orbitrap™ GC-MS System

High-resolution Orbitrap GC-MS system for confident quantitation and unknown screening. Delivers sub-ppb sensitivity, <1 ppm accuracy, and routine full-scan workflows.

<h4><strong>Bringing high-resolution Orbitrap technology into routine GC-MS workflows</strong></h4><p><strong>The Exactive GC Orbitrap system offers the unmatched mass accuracy, resolving power, and sensitivity of Orbitrap technology in a platform optimized for routine applications. This high-performance GC-MS system enables both targeted and non-targeted full-scan workflows with confident quantitation and trace-level detection, even in complex matrices. Designed for maximum robustness and usability, it streamlines operations across food safety, environmental, industrial, forensic, and anti-doping laboratories.</strong></p><h3><strong>Key Features and Benefits</strong></h3><h4>💡 <strong>Unmatched HRAM Performance</strong></h4><ul><li>Resolving power up to <strong>60,000 FWHM at m/z 200</strong></li><li>Mass accuracy: <strong>&lt;1 ppm</strong> (internal), <strong>&lt;3 ppm</strong> (external)</li><li>Quantitative dynamic range: <strong>&gt;10⁶</strong>, with excellent linearity and precision</li><li>Routine IDLs in the <strong>sub-ppb to ppt range</strong></li></ul><h4>🧪 <strong>Full-Scan Sensitivity and Selectivity</strong></h4><ul><li>Combines triple-quad-like quantitation with full-scan Orbitrap capability</li><li>Sub-ppb sensitivity for 100+ pesticides in food matrices</li><li>High selectivity and confidence in trace-level compound identification</li><li>Spectral deconvolution with accurate-mass fragment filtering</li></ul><h4>⚙️ <strong>Proven Orbitrap Design</strong></h4><ul><li>Curved linear trap (C-Trap) and bent flatapole ion optics</li><li>ExtractaBrite™ ion source (EI/PCI/NCI) – removable under vacuum</li><li>Vent-free column exchange using patented source plug</li><li>Optional direct sample probes (DIP, DEP) for solid or thermolabile analytes</li></ul><h4>🧠 <strong>Streamlined Software and Data Processing</strong></h4><ul><li>Controlled by <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/77"><strong>TraceFinder™</strong></a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758"><strong>Xcalibur™</strong></a> platforms</li><li>Full-scan acquisition with automated data mining</li><li>Integrated spectral libraries (NIST, Thermo Food/Environmental)</li><li>Spectral fidelity and mass accuracy retained across wide concentration range</li></ul><h4>🔁 <strong>Optimized GC Integration</strong></h4><ul><li>Built on the <strong>TRACE™ 1310 GC platform</strong></li><li>Supports all injection/detection configurations (SSL, PTV, backflush, etc.)</li><li>Paired with <strong>TriPlus™ RSH autosampler</strong> for advanced robotic sample handling</li><li>Excellent retention time stability and fast oven cycling</li></ul><h3><strong>Ideal For Applications In:</strong></h3><ul><li><strong>Food safety and authenticity</strong> (pesticide screening, additives)</li><li><strong>Environmental analysis</strong> (contaminants, pollutants, air toxics)</li><li><strong>Forensics and toxicology</strong> (unknowns, drugs, metabolites)</li><li><strong>Industrial QC</strong> (extractables, volatiles, impurity profiling)</li><li><strong>Non-targeted screening</strong> with full-scan HRAM and retrospective analysis</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/application::product.product/exactive-gc-orbitrap-gc-ms-front-2000x2000.jpg-650.jpg" alt="Thermo Scientific™ Exactive™ GC Orbitrap™ GC-MS System" width="null" height="null"></p>

Thermo Scientific™ ISQ™ 7000 Single Quadrupole GC-MS System

Robust single quad GC-MS with advanced ion sources, NeverVent™ tech, SmartTune, and flexible software. Delivers sensitive, high-throughput routine analysis.

<h4><strong>Unstoppable GC-MS performance for routine, regulatory, and high-throughput analysis</strong></h4><p><strong>The ISQ 7000 GC-MS system delivers reliable, scalable, and ultra-sensitive performance for routine analysis in food safety, environmental, clinical, and forensic laboratories. Designed to maximize uptime and analytical confidence, it features advanced ion source options, easy maintenance with NeverVent™ technology, and intuitive software tools like SmartTune and AutoSIM.</strong></p><h3><strong>Key Features and Benefits</strong></h3><h4>🚀 <strong>Unstoppable Sensitivity</strong></h4><ul><li>Advanced Electron Ionization (AEI) source for femtogram-level detection</li><li>Robust ExtractaBrite™ ion source, fully removable under vacuum</li><li>Dual-filament design and S-shaped ion optics reduce neutral and chemical noise</li><li>Simultaneous full-scan and SIM acquisition modes</li></ul><h4>⚙️ <strong>Unstoppable Uptime</strong></h4><ul><li><strong>NeverVent™ technology</strong> with Vacuum Probe Interlock (VPI) and V-Lock for maintenance without venting</li><li>Heated ion optics and RF lens protect quadrupole from matrix contamination</li><li>Reliable quadrupole design: solid, non-coated rods and maintenance-free performance</li><li>Up to <strong>98% downtime reduction</strong> for routine interventions (e.g. source cleaning)</li></ul><h4>🧠 <strong>Smart Automation and Ease of Use</strong></h4><ul><li><strong>SmartTune</strong> for guided, fast, and consistent system tuning</li><li><strong>AutoSIM</strong> and <strong>Timed-SIM (t-SIM)</strong> for efficient method creation</li><li><strong>Retention Time Alignment (RTA)</strong> tool for consistent results across column changes</li><li>Deconvolution tools for untargeted compound identification</li></ul><h4>🧪 <strong>Flexible Analytical Capabilities</strong></h4><ul><li>EI, PCI, NCI ionization modes (with optional dual reagent gas switching)</li><li>Mass range: 1.2–1100 u; scanning speed up to 240 scans/s</li><li>Optional Direct Insertion and Direct Exposure probes for solid samples</li><li>Software-switchable ionization modes in a single analysis</li></ul><h4>🖥️ <strong>Integrated Software and Data Handling</strong></h4><ul><li>Full control via <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/76"><strong>Chromeleon™ CDS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/77"><strong>TraceFinder™</strong></a></li><li>Preloaded application libraries and eWorkflows (e.g. US EPA, clinical, toxicology)</li><li>Auto-calibration, QA/QC tools, and compliance-ready (21 CFR Part 11)</li><li>Seamless LIMS connectivity and user-guided reporting tools</li></ul><h4>🔁 <strong>Sample Introduction Options</strong></h4><ul><li>Compatible with <strong>AI/AS 1310</strong>, <strong>TriPlus 300</strong> (HS), and <strong>TriPlus RSH</strong> autosamplers</li><li>Robotic workflows including dilution, standard addition, and derivatization</li><li>Ideal for trace analysis in complex matrices and high-throughput environments</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/application::product.product/isq-7000-trace-1310-triplus-rsh-left-1200x1200.jpg-650_s.webp" alt="Thermo Scientific™ ISQ™ 7000 Single Quadrupole GC-MS System" width="382" height="382"></p>

Thermo Scientific™ TSQ™ 9000 GC-MS/MS System

Triple quadrupole GC-MS/MS system offering exceptional sensitivity, robustness, and smart automation for trace-level quantitation in food, environmental, and forensic analysis.

<p><strong>High-performance triple quadrupole GC-MS/MS platform for routine trace-level quantitation in complex matrices</strong></p><p>The <strong>TSQ™ 9000 GC-MS/MS system</strong> is built for laboratories that demand <strong>uncompromising sensitivity, throughput, and robustness</strong> for trace-level analysis in <strong>food safety, environmental, and forensic/toxicology applications</strong>. Designed to increase productivity and reduce cost-per-sample, this system delivers best-in-class performance with intuitive tools for routine and advanced users alike.</p><h3><strong>Key features and benefits</strong></h3><h4>🚀 <strong>Unstoppable Sensitivity</strong></h4><ul><li><strong>Advanced Electron Ionization (AEI)</strong> source for attogram-level sensitivity, ideal for demanding applications like dioxins and pesticides in baby food.</li><li><strong>ExtractaBrite™ ion source</strong>: robust EI/CI performance, proven in routine use; fully removable under vacuum with NeverVent™.</li><li><strong>Ionization modes: </strong>EI, PCI, NCI (CI available with ExtractaBrite only)</li></ul><h4>🔬 <strong>Unstoppable Performance</strong></h4><ul><li><strong>EvoCell™ collision cell</strong>: up to 800 SRM transitions/s with excellent precision</li><li><strong>0.5 ms minimum dwell time</strong>; up to 97 scans/s; 20,000 u/s quadrupole scan speed</li><li><strong>Mass range</strong>: 1.2–1100 u, unit mass resolution</li><li><strong>Mass stability</strong>: &lt;0.1 u over 48 h</li></ul><h4>🔧 <strong>Unstoppable Uptime</strong></h4><ul><li><strong>NeverVent™ technology</strong> (with VPI &amp; ExtractaBrite): perform maintenance or column changes without venting</li><li><strong>V-Lock</strong> column isolation prevents system venting during GC maintenance</li><li><strong>Dual heated source lenses</strong> for minimal contamination and high matrix tolerance</li></ul><h3><strong>Ease of Use and Intelligent Method Development</strong></h3><ul><li><strong>AutoSRM™</strong>: Automated SRM development wizard</li><li><strong>SIM Bridge™</strong>: Convert legacy SIM methods into SRM in seconds</li><li><strong>Timed-SRM and Retention Time Alignment (RTA)</strong>: Maintain optimal sensitivity and peak tracking across injections</li><li><strong>SmartTune™</strong>: Intuitive system tuning with error guidance</li></ul><h3><strong>Software Platforms</strong></h3><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76"><strong>Chromeleon™ CDS</strong></a></h4><ul><li>Fully integrated control of chromatography and mass spectrometry</li><li>One-click eWorkflows™, Intelligent Run Control, automated compliance tools</li><li>Environmental Analysis Extension Pack for U.S. EPA workflows</li></ul><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/77"><strong>TraceFinder™</strong></a></h4><ul><li>Comprehensive solution for method development, acquisition, processing, and reporting</li><li>Preloaded SRM database for over 1300 pesticides (environmental/food safety versions)</li><li>Application-specific variants for <strong>clinical research</strong>, <strong>toxicology</strong>, and <strong>environmental labs</strong></li></ul><h3><strong>System Specifications</strong></h3><ul><li><strong>Ion sources</strong>: AEI and ExtractaBrite (CI optional)</li><li><strong>Transfer line temperature</strong>: up to 400 °C</li><li><strong>Detector</strong>: DynaMax™ XR system with off-axis dynode and wide dynamic range (&gt;10⁷)</li><li><strong>Mass resolution</strong>: Adjustable 0.4–2.5 u, preset or manual</li><li><strong>Pumping system</strong>: High-capacity turbomolecular with rotary vane or optional oil-free scroll</li><li><strong>Direct Probe Options (VPI only)</strong>: DIP and DEP probes for solid and rapid samples</li></ul><h3><strong>Sample Introduction Options</strong></h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/68"><strong>AI/AS 1310 Autosampler</strong></a>: high-precision injections, advanced rinse protocols</li><li><strong>TriPlus™ RSH Autosampler</strong>: robotic automation including dilution, internal standard addition, derivatization, and tool switching</li></ul><h3><strong>System Dimensions and Compatibility</strong></h3><ul><li>Fully compatible with <strong>Thermo Scientific™ TRACE™ 1300/1310 GC</strong></li><li><strong>Mass spectrometer</strong>: 44 × 40 × 89 cm; 61 kg</li><li>Modular design enables scalable performance and easy upgrades</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/application::product.product/Thermo-TSQtm-9000-1310-triplus-rsh_l.webp" alt="Thermo Scientific™ TSQ™ 9000 GC-MS/MS System" width="708" height="708"></p>

Products focused on GC/MSD distributed by Thermo Fisher Scientific (EMEA) - page 2

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