<p><strong>Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal </strong><i><strong>Nature Chemical Biology</strong></i><strong>.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.</p><p>In their study,<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en"><strong>Dr. Hana Cahová</strong></a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.</p><p>The work was significantly shaped by <strong>Valentina Serianni from Hana Cahová’s team</strong>, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by <strong>Jana Škerlová</strong>, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.</p><p>Hana Cahová adds: <i>“We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”</i></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by<strong> Dr. Tomáš Kouba</strong>, one of the authors of the study. <i>“Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,”</i> he explains.</p><p>Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en"><strong>cryo-EM center</strong></a><strong> </strong>that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"></p><blockquote><h4><strong>The Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5"><strong>Molecular insight into 5′ RNA capping with Np<sub>n</sub>Ns by bacterial RNA polymerase</strong></a></h4><p>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;</p><p>Nat. Chem. Biol. 21 (2026)</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from <i>Thermus</i> <i>thermophilus</i>. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</p></div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

<p><strong>In the week of February 9, the following webinars await you in the field of gas / solid phase and especially Raman, FTIR, GC and GC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><div><div><div><div><div><div><h5><strong>1. Thermo Fisher Scientific: Process Raman – A Reliable and Versatile Tool for Downstream Process Monitoring and Control for mAb Production</strong></h5><ul><li>Tu, 10.2.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/5808">Registration</a></li></ul><p>The presentation highlights how process Raman spectroscopy offers several advantages over traditional in-line UV-Vis analysis for process monitoring and metabolite production control in mAb manufacturing.</p><h5><strong>2. Wasson-ECE Instrumentation: The Data Driven Plant – How AI Transforms Chemical Manufacturing</strong></h5><ul><li>Tu, 10.2.2026 21:30 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/5744">Registration</a></li></ul><p>Explore how artificial intelligence is applied to improve process efficiency, monitoring, and decision-making in modern chemical manufacturing.</p><h5><strong>3. New Food Magazine: The Science of Next-Gen Ingredients – Fermentation, Formulation and Function</strong></h5><ul><li>We, 11.2.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/5720">Registration</a></li></ul><p>Discover how fermentation-driven innovation is transforming functional food ingredients, with analytical insights to optimize flavour, functionality, and product stability.</p></div></div></div></div></div></div>

Webinars LabRulezGCMS Week 07/2026

<p><strong>This study presents an analytical strategy combining QuEChERS extraction with low-temperature partitioning (LTP) for the determination of multiclass pesticide residues in cocoa beans by GC–MS. Extraction conditions were optimized using a fractional factorial design, resulting in a simple and efficient workflow suitable for complex cocoa matrices.</strong></p><p>The validated method showed satisfactory limits of detection and quantification, recoveries within 70–120%, and good overall performance. Application to cocoa samples from Brazil detected permethrin in several samples, demonstrating that the QuEChERS–LTP approach is an effective and reliable cleanup strategy for routine pesticide residue analysis in cocoa.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c10360"><strong>QuEChERS Combined with Low-Temperature Partitioning and GC–MS as an Analytical Strategy for the Determination of Multiclass Pesticide Residues in Cocoa Beans</strong></a></h4><p>Priscilla M. de Freitas Machado, Madson M. Nascimento, Paulo R. R. Mesquita, Manuela B. Nascimento, Lilian Lefol N. Guarieiro, Gisele O. da Rocha, Jailson B. de Andrade, Raildo M. de Jesus*</p><p>ACS Omega 2025, 10, 51, 63464–63473</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acsomega.5c10360">https://doi.org/10.1021/acsomega.5c10360</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><p>Cocoa is best known as the primary raw material for chocolate but is also processed into products such as cocoa butter, biscuits, cocoa honey, and cocoa powder. Cocoa powder is derived from roasted, pressed, and milled nibs, containing on average 20% (w/w) lipids on a dry basis and less than 9% moisture. <a href="javascript:void(0);">(3,4)</a></p><p>According to the Brazilian Health Regulatory Agency (ANVISA), pesticides such as atrazine, bifenthrin, cyproconazole, metalaxyl, tebuconazole, trifloxystrobin, and permethrin are approved for use in Brazil. <a href="javascript:void(0);">(13)</a> However, among these, only metalaxyl, trifloxystrobin, and tebuconazole are authorized by the European Union. <a href="javascript:void(0);">(14)</a> Thus, investigating these contaminants in cocoa samples is of utmost importance, as Brazil is one of the largest cocoa exporters, supplying cocoa commodities to South America, the United States, and the European Union. <a href="javascript:void(0);">(2)</a> Furthermore, Organochlorine pesticides, in particular, belong to the class of persistent organic pollutants (POPs). Although banned in many countries, they continue to be used in some developing nations because of their chemical stability, resistance to degradation, volatility, and high lipophilicity, which promote bioaccumulation and neurotoxicity. <a href="javascript:void(0);">(15)</a> Epidemiological evidence also associates their exposure with Parkinson’s disease, certain cancers, diabetes, and endometriosis. <a href="javascript:void(0);">(16)</a></p><p>Residues of pesticides in cocoa have been reported in various studies. <a href="javascript:void(0);">(17)</a> Analyzing pesticide residues in cocoa presents analytical challenges due to the matrix’s complexity, comprising high lipid content, fatty acids and esters, sugars, polyphenols, and caffeine. These components can interfere with extraction efficiency and contaminate analytical instrumentation. <a href="javascript:void(0);">(18)</a> Therefore, efficient sample preparation methods incorporating effective cleanup steps are essential for accurate residue determination.</p><p>Several techniques have been employed for multiresidue pesticide analysis in cocoa. For instance, Idowu et al. <a href="javascript:void(0);">(19)</a> quantified 14 organochlorines using Soxhlet extraction and silica/Na<sub>2</sub>SO<sub>4</sub> cleanup, with GC ECD detection. Okoffo et al. <a href="javascript:void(0);">(20)</a> determined 13 organophosphates and 9 pyrethroids employing SPE cartridges (Envi-carb/LC-NH<sub>2</sub> and Bond Elute C18), acetonitrile extraction, and GC PFPD or GC ECD quantification. Yusiasih et al. <a href="javascript:void(0);">(21)</a> applied dispersive SPE with PSA, Florisil, and MgSO<sub>4</sub> for pyrethroids, followed by GC ECD and GC–MS.</p><p>Therefore, this study aimed to evaluate the effectiveness of a QuEChERS–LTP analytical procedure for the determination of multiclass pesticide residues in cocoa beans. The factors that affect the QuEChERS–LTP procedure efficiency were optimized. After validation, the procedure was employed to investigate the occurrence of 15 multiclass pesticides in cocoa samples.</p><h4><strong>Experimental Section</strong></h4><h5><strong>Instrumental Apparatus</strong></h5><p>Pesticide separation and identification were performed on a Shimadzu <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=1&amp;search=GC-MS-QP2010SE"><strong>GC-MS-QP2010SE</strong></a> system (Kyoto, Japan) equipped with an <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=1&amp;search=AOC-20i"><strong>AOC-20i autosampler</strong></a>. Separation was achieved using an <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=10&amp;search=DB-5MS"><strong>Agilent DB-5MS capillary column</strong></a> (30 m × 0.25 mm × 0.25 μm) with a stationary phase of 5% phenyl/95% dimethylpolysiloxane. The instrumental conditions were based on Nascimento et al. <a href="javascript:void(0);">(49)</a> and they are summarized in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c10360#tbl4">Table 4</a>.</p><p>For quantification, the base peak was used, while two additional ions were monitored for confirmation (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acsomega.5c10360/suppl_file/ao5c10360_si_001.pdf">Table S2</a>) in accordance with SANTE guidelines. <a href="javascript:void(0);">(50)</a> A GC–MS/SIM chromatogram illustrating the separation of all target pesticides is presented in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c10360#fig5">Figure 5</a>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_51_63464_63473_Figure_5_Extracted_ion_chromatogram_EIC_in_SIM_mode_of_the_target_pesticides_f2461c14f4.jpg" alt="ACS Omega 2025, 10, 51, 63464–63473: Figure 5. Extracted ion chromatogram (EIC) in SIM mode of the target pesticides obtained from the injection of a mixed standard solution at 100 μg L–1. Analytes are listed in order of elution: [1] Molinate (6.18 min); [2] α-HCH (8.15 min); [3] atrazine-d5 (8.69 min); [4] atrazine (8.75 min); [5] diazinon (9.31 min); [6] disulfoton (9.67 min); [7] dimethachlor (10.60 min); [8] metalaxyl (11.25 min); [9] p,p′-DDE (15.82 min); [10] cyproconazole I (16.50 min); [11] cyproconazole II (16.56 min); [12] p,p′-DDD (17.29 min); [13] ethion (17.34 min); [14] trifloxystrobin (18.43 min); [15] tebuconazole (19.13 min); [16] bifenthrin (20.47 min); [17] permethrin I (23.92 min); [18] permethrin II (24.18 min)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_51_63464_63473_Figure_5_Extracted_ion_chromatogram_EIC_in_SIM_mode_of_the_target_pesticides_f2461c14f4.jpg 245w," sizes="100vw" width="2080" height="835"></p><h5><strong>Application of the Proposed Procedure to Real Samples</strong></h5><p>In a 50 mL Falcon tube, 2.0 g of sample was weighed using an analytical balance (AUX320, Shimadzu, Kyoto, Japan). A total of 2.0 g of salt (NaCl and Na<sub>2</sub>SO<sub>4</sub> in a 1:1 w/w ratio) was added, followed by an aliquot of the atrazine-<i>d</i><sub>5</sub> solution (surrogate standard) to yield a final concentration of 250 μg kg<sup>–1</sup>. A mixture of acetonitrile (8.0 mL) and ultrapure water (2.0 mL) was then added. The system was vortexed (K40-10208, Kasvi, São Paulo, Brazil) for 3.0 min at 2500 rpm and centrifuged (MPW-351R, MPW Med. Instruments, Warsaw, Poland) for 10.0 min at 15 °C and 10,000 rpm. After phase separation, approximately 6.0 mL of the supernatant was transferred to a Falcon tube containing 150.0 mg of PSA sorbent. The mixture was vortexed again for 3.0 min at 2500 rpm and centrifuged for an additional 10.0 min under the same conditions. The tubes were then placed in the freezer for 24 h. Once the aqueous phase had frozen, the organic extract containing the analytes was collected, filtered through a Cytiva Whatman MiniUniprep G2 vial with a 0.20 μm membrane filter (Marlborough, Massachusetts, USA), and injected into the GC–MS system (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c10360#fig6">Figure 6</a>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_51_63464_63473_Figure_6_Schematic_representation_of_the_extraction_procedure_using_the_Qu_E_Ch_ERS_LTP_procedure_for_pesticide_extraction_in_cocoa_powder_samples_ed58549bcf.jpg" alt="ACS Omega 2025, 10, 51, 63464–63473: Figure 6. Schematic representation of the extraction procedure using the QuEChERS–LTP procedure for pesticide extraction in cocoa powder samples." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_51_63464_63473_Figure_6_Schematic_representation_of_the_extraction_procedure_using_the_Qu_E_Ch_ERS_LTP_procedure_for_pesticide_extraction_in_cocoa_powder_samples_ed58549bcf.jpg 223w," sizes="100vw" width="1458" height="1022"></p><h4><strong>Results and Discussion</strong></h4><h5><strong>Evaluation of the Effect of Low and Ultralow Temperatures on the Analytical Response</strong></h5><p>Studies reporting the use of LTP generally employ a conventional freezer, which typically reaches −18 ± 2 °C as the minimum temperature. In this equipment, effective phase separation can require 8 to 24 h. <a href="javascript:void(0);">(35−37)</a> In contrast, ultralow temperature freezers (ultrafreezers) can reach −80 °C, leading to a substantial reduction in partitioning time. In this study, tests performed with the conventional freezer achieved satisfactory partitioning after 24 h, whereas in the ultrafreezer, complete partitioning was observed in only 20 min.</p><p>Considering the analytical response in terms of peak area (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c10360#fig3">Figure 3</a>), higher signals were obtained when LTP was performed in a conventional freezer rather than an ultrafreezer. This behavior can be attributed to the partitioning mechanism. <a href="javascript:void(0);">(32)</a> Partitioning depends on cooling and the subsequent freezing of the aqueous phase. In the conventional freezer, the slower freezing rate provides sufficient time for the system to approach thermodynamic equilibrium, favoring analyte diffusion between phases. Under these conditions, analyte molecules have more time to migrate and distribute optimally before complete solidification. In contrast, the rapid freezing in the ultrafreezer may trap analytes in a nonideal distribution, reducing the efficiency of the partitioning process <a href="javascript:void(0);">(32)</a> (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c10360#fig3">Figure 3</a>). According to these observations, the conventional freezer was selected for subsequent experiments.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_51_63464_63473_Figure_3_Comparison_of_chromatographic_peak_areas_of_pesticides_after_partitioning_in_the_conventional_freezer_versus_the_ultrafreezer_9598bcfec3.jpg" alt="ACS Omega 2025, 10, 51, 63464–63473: Figure 3. Comparison of chromatographic peak areas of pesticides after partitioning in the conventional freezer versus the ultrafreezer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_51_63464_63473_Figure_3_Comparison_of_chromatographic_peak_areas_of_pesticides_after_partitioning_in_the_conventional_freezer_versus_the_ultrafreezer_9598bcfec3.jpg 175w," sizes="100vw" width="996" height="891"></p><h5><strong>Application to Real Samples</strong></h5><p>Fifteen samples were analyzed in triplicate, comprising two from the State of Pará and 12 from the State of Bahia, located in the North and Northeast regions of Brazil, respectively. Among the 15 pesticides investigated, only permethrin isomers were quantified in four samples (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c10360#tbl3">Table 3</a>). The herbicide molinate was also detected in three samples; however, its concentration was below LOQ. Permethrin isomers concentrations ranged from 17.1 ± 0.3 to 49.6 ± 1.8 μg kg<sup>–1</sup>. These values are below the MRL established by European Union regulation, which is 100 μg kg<sup>–1</sup>. <a href="javascript:void(0);">(42)</a> Although permethrin is not permitted in the European Union, <a href="javascript:void(0);">(42)</a> it remains authorized for use as insecticide in Brazil, particularly in crops such as cotton, rice, coffee, citrus, cabbage, beans, tobacco, maize, soybean, tomato, wheat, and grape. <a href="javascript:void(0);">(13)</a> However, no MRL values have been established for cocoa beans under Brazilian legislation. <a href="javascript:void(0);">(13)</a> <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c10360#tbl3">Table 3</a> shows the concentration of detected compounds in the cocoa samples.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_51_63464_63473_Table_3_Mean_Concentrations_mg_kg_1_Standard_Deviation_of_Target_Pesticides_Detected_in_This_Study_2427955699.jpg" alt="ACS Omega 2025, 10, 51, 63464–63473: Table 3. Mean Concentrations (μg kg–1 ± Standard Deviation) of Target Pesticides Detected in This Study" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_51_63464_63473_Table_3_Mean_Concentrations_mg_kg_1_Standard_Deviation_of_Target_Pesticides_Detected_in_This_Study_2427955699.jpg 196w," sizes="100vw" width="1070" height="851"></p><p>To assess method performance for pesticides not detected in the cocoa samples, one of the analyzed samples was fortified with a mixed standard solution at concentrations of 50, 250, and 500 μg kg<sup>–1</sup>. The fortified analytes were subsequently detected in the chromatograms presented in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acsomega.5c10360/suppl_file/ao5c10360_si_001.pdf">Figures S2–S4</a>, indicating good response of the proposed method.</p><h4><strong>Conclusions</strong></h4><p>The QuEChERS–LTP procedure was successfully developed for the determination of 16 multiclass pesticide residues in cocoa beans. For the first time, ultralow temperature (−80 °C) was applied to accelerate the low-temperature partitioning process during pesticide extraction from cocoa beans. Nevertheless, conventional freezing at −12 °C proved to be more effective for the extraction of the target analytes.</p><p>Evaluation of the matrix effect demonstrated that matrix-matched analytical curves, constructed in an analyte-free cocoa bean extract, were more effective in compensating for matrix interferences than the use of analyte protectants.</p><p>The pyrethroid permethrin was quantified in four cocoa samples. However, the concentrations did not exceed the MRL established by European Union legislation.</p><p>Overall, the QuEChERS–LTP procedure proved to be a promising approach for pesticide residue determination, as it requires smaller volumes of solvents and reagents, involves fewer cleanup and extraction steps, and shows strong potential for application to other complex matrices.</p>

QuEChERS Combined with Low-Temperature Partitioning and GC–MS as an Analytical Strategy for the Determination of Multiclass Pesticide Residues in Cocoa Beans

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The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

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<div><div id="product-description"><p><strong>Enhance liquid sampling automation for your gas chromatography (GC) applications with simplicity and robustness. Designed to meet the demands of any busy high-throughput lab, the Thermo Scientific™ AI/AS 1610 Liquid Autosampler will help to process more samples efficiently and safely with its advanced design and usability features, greatly enhanced for easy adoption for both beginners and experienced users.&nbsp;</strong></p><p>With the possibility to expand to <strong>up to a 310-sample capacity </strong>with <strong>simultaneous dual-tower operations</strong>, the AI/AS 1610 offers the fit-for-purpose solution to budget-conscious laboratories looking for reliable automation of liquid samples injection into a<strong> GC or GC-MS system. </strong>The Thermo Scientific™ AI/AS 1610 Liquid Autosampler combine <strong>performance, simplicity, and robustness for your everyday workflow.</strong></p><h5><strong>The AI/AS 1610 Liquid Autosampler</strong> is available in <strong>two models:&nbsp;</strong></h5><ul><li>the upgradable<strong> 8-vial capacity AI 1610</strong>&nbsp;</li><li>the<strong> 155-vial capacity AS 1610&nbsp;</strong></li></ul><p>Both offer the<strong> dual-tower Gemini configuration for simultaneous injection in two channels.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Autosampler_AI_1610_s_kapacitou_8_nebo_155_vzorku_9d37f0ea9a/Pragolab-Autosampler-AI-1610-s-kapacitou-8-nebo-155-vzorku.jpg" alt="Thermo Scientific™ AI/AS 1610 Liquid Autosampler:  8-vial capacity and 155-vial capacity AI 1610 " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Autosampler_AI_1610_s_kapacitou_8_nebo_155_vzorku_9d37f0ea9a/thumbnail_Pragolab-Autosampler-AI-1610-s-kapacitou-8-nebo-155-vzorku.jpg 245w," sizes="100vw" width="1097" height="666"></p><h3><strong>Enhanced Usability</strong></h3><ul><li><strong>Easy set up</strong> through self-aligning, not requiring any position adjustment</li><li><strong>Easy slide-in installation and free access to the injector </strong>for maintenance</li><li><strong>Easy syringe viewing and replacement</strong> with illuminated compartment and magnifier lens</li><li>Enhanced <strong>color-coded instrument status indication</strong></li><li><strong>Safe operation</strong> with automatic selection of the best injection settings through recognition of the injector type and injection mode</li><li><strong>Simplified connectivity </strong>with a single cable including communication, synchronization and power supply</li></ul><h3><strong>Enhanced Sample Throughput</strong></h3><ul><li><strong>Wide sample capacity scalability</strong> to fit any workload demand</li><li><strong>Dual Tower Gemini</strong> for upmost productivity with simplified configuration and connectivity</li></ul><h3><strong>Enhanced Performance</strong></h3><ul><li>Expanded applicability with liquid sample volume <strong>injections up to 80 μL</strong></li><li>Free pre- and post injection syringe rinsing with single or combined solvents (up to four) virtually <strong>eliminates sample carry-over</strong></li><li><strong>Efficient, accurate sampling</strong> <strong>of low boiling compounds</strong> with the syringe kept away from the inlet’s temperature influence</li><li><strong>Seamless integration into Thermo Scientific™ chromatography software</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Dizajn_autosampleru_Thermo_AI_AS_1610_pro_snadny_pristup_k_injektoru_GC_0b1d294ab5/Pragolab-Dizajn-autosampleru-Thermo-AI-AS-1610-pro-snadny-pristup-k-injektoru-GC..jpg" alt="Thermo Scientific™ AI/AS 1610 Liquid Autosampler" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Dizajn_autosampleru_Thermo_AI_AS_1610_pro_snadny_pristup_k_injektoru_GC_0b1d294ab5/thumbnail_Pragolab-Dizajn-autosampleru-Thermo-AI-AS-1610-pro-snadny-pristup-k-injektoru-GC..jpg 245w," sizes="100vw" width="1090" height="520"></p><h3><strong data-start="105" data-end="123">Specifications</strong></h3><ul><li><strong data-start="126" data-end="146">Sample Capacity:</strong> 8 x 2 mL Vials</li><li><strong data-start="164" data-end="183">Injection Mode:</strong> Automatically selected and optimized according to injector type</li><li><strong data-start="250" data-end="270">Injection Speed:</strong> Fast/Slow/Variable (1 to 60,000.00 μL/min)</li><li><strong data-start="317" data-end="338">Injection Volume:</strong> 0.01 to 80 μL</li><li><strong data-start="355" data-end="371">Description:</strong> AI 1610 Liquid Autosampler for TRACE 1600 Series GC</li><li><strong data-start="426" data-end="447">Washing Solvents:</strong> Up to 4</li><li><strong data-start="458" data-end="471">Quantity:</strong> Each</li><li><strong data-start="479" data-end="493">Unit Size:</strong> Each</li></ul><figure class="image"><img style="aspect-ratio:650/434;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_AI_AS_1610_Liquid_Autosampler_37e5c32f69.jpg" alt="Thermo Scientific™ AI/AS 1610 Liquid Autosampler" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_AI_AS_1610_Liquid_Autosampler_37e5c32f69.jpg 234w," sizes="100vw" width="650" height="434"></figure></div></div>

Thermo Scientific™ TriPlus™ RSH Autosampler

Robotic GC autosampler with multimode injection, tool exchange, and full sample prep automation. Enables 24/7 workflows for food, environmental, and forensic labs.

<h4><strong>Flexible, high-capacity autosampler for GC and GC-MS with advanced sample prep automation</strong></h4><p><strong>The TriPlus RSH Autosampler is a versatile robotic sampling platform designed to maximize automation, precision, and productivity in gas chromatography workflows. Fully compatible with Thermo Scientific™ GC and GC-MS systems, it supports liquid, headspace (HS), and SPME/SPME Arrow injections, with optional modules for µSPE, ITEX-DHS, and automated sample preparation. The unique Automatic Tool Change (ATC) system allows the autosampler to switch between up to six syringes in a single sequence, enabling unattended workflows for complex multistep protocols.</strong></p><h3><strong>Key Features and Benefits</strong></h3><h4>🧪 <strong>Multimode Injection and Sample Handling</strong></h4><ul><li>Supports liquid, headspace, SPME, SPME Arrow, and ITEX-DHS techniques</li><li>Switch between injection modes in a single sequence using ATC</li><li>Bottom vial sensing enables precise injections from as little as <strong>5 μL</strong> of sample</li></ul><h4>🔁 <strong>Automated Sample Preparation</strong></h4><ul><li>Perform dilution, standard addition, derivatization, and internal standard workflows</li><li>Tools: Vortexer, Incubator, Heatex-Stirrer, Dilutor, µSPE module</li><li>Visual drag-and-drop <strong>Sampling Workflow Editor</strong> for custom prep protocols</li></ul><h4>📦 <strong>High Throughput and Capacity</strong></h4><ul><li>Unattended operation with up to <strong>972 × 2 mL vials</strong> or <strong>840 × 0.7 mL vials</strong></li><li>Extended X-arm option for larger trays and accessory support</li><li>Dual-GC configurations with Clone Mode or DoublePro setups</li></ul><h4>⚙️ <strong>Precision and Flexibility</strong></h4><ul><li>Automated Tool Change (ATC): Up to <strong>6 tools</strong> in one sequence</li><li>Injection volume range: <strong>0.1 μL to 10 mL</strong></li><li>Compatible with all major GC injector types (SSL, PTV, OC, PKD, PPKD)</li></ul><h4>🧠 <strong>Smart Software and Control</strong></h4><ul><li>Fully integrated with <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/76"><strong>Chromeleon™</strong></a>,<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758"><strong> Xcalibur™</strong></a>, and <strong>Chrom-Card™ CDS</strong></li><li>Local controller or virtual terminal control interface</li><li>Barcode readers for full sample tracking</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/application::product.product/Thermo-TriPlus-RSH-Autosampler-1.jpg" alt="Thermo Scientific™ TriPlus™ RSH Autosampler" width="null" height="null"></p><h3><strong>Applications</strong></h3><ul><li><strong>Food safety</strong>: Trace analytes, contaminants, derivatization (e.g. FAMEs, dioxins)</li><li><strong>Environmental</strong>: VOCs, SVOCs, POPs, air toxics</li><li><strong>Forensics &amp; clinical</strong>: Biological matrices, explosive residues, drugs</li><li><strong>Chemical industry</strong>: Routine QC, trace impurities, residual solvents</li><li><strong>High-throughput labs</strong>: 24/7 unattended workflows with complex prep steps</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/application::product.product/QExactive-GCMSMS-Orbitrap-with-Autosampler-sideangle-1856x1391.jpg-650.jpg" alt="Thermo Scientific™ TriPlus™ RSH Autosampler" width="null" height="null"></p>

Thermo Scientific TRACE 1310 GC Analyzers for Aromatics in Fuels

TRACE 1310 GC analyzers for BTEX in fuels enable fast, standard-compliant analysis of aromatics and oxygenates in gasoline. Preconfigured, tested, and automation-ready.

<div><p><strong>Easily analyze single or multiple spark ignition fuel samples using the Thermo Scientific™ TRACE™ 1310 GC Analyzers for aromatics (BTEX) in fuels. These automated gas chromatography systems measure and report aromatics, benzene, toluene, etc. in spark ignition fuels, such as gasoline.</strong></p><p>They are preconfigured, method tested, and ready to analyze samples after installation, using the<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products/76"><strong>Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)</strong></a> to acquire and analyze data. Each system is delivered with methods pre-installed and a complete documentation package.</p><div><p><strong>Three TRACE 1310 gas chromatography systems are available</strong>. Each is configured for analyzing gasoline (spark ignition fuels) according to ASTM standard test methods.</p><h4><strong>⚙️ TRACE 1310 GC for benzene and toluene by ASTM D3606</strong></h4><p>A thermal conductivity detector (TCD) detects and quantitates aromatics in spark ignition fuels such as gasoline and aviation fuels specified in ASTM D3606. The stripper column holds hydrocarbons up for backflush to prevent contamination of aromatics column.</p><h4><strong>⚙️ TRACE 1310 GC for aromatics by ASTM D5580</strong></h4><p>An FID channel detects and quantitates finished gasoline as specified in ASTM D7754. The stripper column holds non-aromatic hydrocarbons, which are backflushed to vent to prevent background contamination.</p><h4><strong>⚙️ TRACE 1310 GC for gasoline by ASTM D4815, 3606, and 5580</strong></h4><p>A combination analyzer for gasoline, it analyzes for oxygenates or aromatics. An FID channel detects and quantitates oxygenate compounds in spark ignition fuels (gasoline) as specified in the ASTM method D4815. Includes TCD channel to detect aromatic hydrocarbons by ASTM 3606. The stripper column holds hydrocarbons, which are vented by a pressure point tee to prevent contamination of oxygenates column.</p><h3><strong>🔧&nbsp;Built to maximize productivity and uptime</strong></h3><ul><li>Systems features Instant Connect detector modules increasing uptime with detector exchange in minutes</li><li>Heated sample bulkheads ensure complete sample transfer</li><li>Automated injection for liquid samples</li><li>Optimized system footprint to reduce benchspace requirement</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/application::product.product/Thermo-TRACEtm-1310-GC-Analyzers-for-Aromatics-in-Fuels.jpg" alt="Thermo Scientific TRACE 1310 GC Analyzers for Aromatics in Fuels" width="null" height="null"></p></div></div>

Thermo Scientific TRACE 1310 GC Natural Gas (NG) and Natural Gas Liquid (NGL) Analyzers

Choose from 15+ TRACE 1310 NG/NGL analyzers for standard-compliant analysis of BTU content, speciation, and impurities. Options from single to multi-channel systems.

<div><div><p><strong>Select from over 15 turnkey Thermo Scientific™ TRACE™ 1310 Natural Gas and Natural Gas Liquid Analyzers for the vital information you need to meet increasing need for analytical data as natural gas (NG) and natural gas liquid (NGL) demand, production, custody transfer, and consumption.</strong></p><p><strong>TRACE 1310 NG/NGL systems </strong>conduct analyses according to standard methods, GPA, ASTM, ISO, DIN, including calorific or BTU content, hydrocarbon speciation and impurities. Choose from low-cost single channel, best-value dual channel, or highly flexible combination systems.</p></div><div><h3><strong>⚙️&nbsp;Choice of Turnkey NG/NGL Analyzers</strong></h3><p>Our team of specialist, with over 60 years in the business, has developed a complete line of analyzers. We offer over 15 different combinations to select from and all feature the innovative modularity of our Instant Connect injectors and detectors, providing the highest uptime and easiest maintenance.</p><h4><strong>Single channel analyzers – Lowest priced systems</strong></h4><ul><li>Analyze a single sample per analysis using a single channel analyzer for NG or NGL.</li></ul><h4><strong>Dual channel analyzers – Best value systems</strong></h4><ul><li>Run two different samples simultaneously for improved productivity, or one sample with two channels of data BTU and speciation.</li></ul><h4><strong>Combination analyzers – Most flexible system</strong></h4><ul><li>A single platform capable of performing all the standard methods on a NG or NGL sample.</li></ul><h3><strong>🔧&nbsp;Common System Features</strong></h3><ul><li><strong>Gas Chromatograph: </strong>TRACE 1310 Gas Chromatography system with 12cm color touch screen</li><li><strong>Column Oven:</strong> Forced air heating with option cryo cooling</li><li><strong>Max Temp: </strong>450°C</li><li><strong>Maximum Heating Rate: </strong>125°C/min</li><li><strong>Heated Zones:</strong> 6</li><li><strong>Detector: </strong>Thermal conductivity detector (TCD)</li><li><strong>Filaments:</strong> Dual Ni/Fe</li><li><strong>Sensitivity: </strong>Hydrocarbons 0.005%, Permannet Gases 0.01%, H2S 0.05%</li><li><strong>Linear Dynamic Range:</strong> 1 x 105</li><li><strong>Max Temperature:</strong> 400°C</li><li><strong>Dimensions (HxWxD cm):</strong> 45 x 53.5 x 67</li><li><strong>Weight (kg):</strong> 41</li><li><strong>Voltage: </strong>120V +/- 10%</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_TRACE_1310_GC_Natural_Gas_NG_and_Natural_Gas_Liquid_NGL_Analyzers_03a7b3a3f4.jpg" alt="Thermo Scientific TRACE 1310 GC Natural Gas (NG) and Natural Gas Liquid (NGL) Analyzers: Natural Gas Sample – C6+ Early Regroup with Oxygen/Nitrogen Separation." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_TRACE_1310_GC_Natural_Gas_NG_and_Natural_Gas_Liquid_NGL_Analyzers_03a7b3a3f4.jpg 245w," sizes="100vw" width="1096" height="522"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_TRACE_1310_GC_Natural_Gas_NG_and_Natural_Gas_Liquid_NGL_Analyzers_2_c65fde10cb.jpg" alt="Thermo Scientific TRACE 1310 GC Natural Gas (NG) and Natural Gas Liquid (NGL) Analyzers: Natural Gas Liquid Sample – C7+ Early Regroup." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_TRACE_1310_GC_Natural_Gas_NG_and_Natural_Gas_Liquid_NGL_Analyzers_2_c65fde10cb.jpg 245w," sizes="100vw" width="1057" height="510"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_TRACE_1310_GC_Natural_Gas_NG_and_Natural_Gas_Liquid_NGL_Analyzers_3_23aa8bf3a2.jpg" alt="Thermo Scientific TRACE 1310 GC Natural Gas (NG) and Natural Gas Liquid (NGL) Analyzers: Extended Chromatogram for GPA 2186 (inset zoom of C6+)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_TRACE_1310_GC_Natural_Gas_NG_and_Natural_Gas_Liquid_NGL_Analyzers_3_23aa8bf3a2.jpg 203w," sizes="100vw" width="1065" height="817"></p></div></div>

Thermo Scientific TRACE 1310 GC Analyzers for Permanent Gases & Trace Impurities

Choose TRACE 1310 GC systems for industrial gas stream analysis. From labs to at-line use, they’re pre-configured, tested, and ideal for permanent gases, CO/CO₂, and impurities.

<div><p><strong>Refuse to compromise by choosing from our variety of</strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products?category=9&amp;instrumentationItems=1&amp;manufacturerItems=3&amp;search=TRACE%E2%84%A2+1310"><strong> Thermo Scientific™ TRACE™ 1310 gas chromatography systems</strong></a><strong> for the analysis of industrial gas streams.&nbsp;</strong></p><p>These systems can be used in laboratories and in pilot/scale-up to at-line environments. Whether you need to analyze permanent gases, hydrogen, low levels of CO/CO<sub>2</sub>, impurities in chlorine, trace-level impurities in pure gases or a combination of those analyses, there is a system to meet your requirements. TRACE 1310 analyzers for industrial gas streams come pre-configured, factory tested and ready to connect to your lab services.</p><div><h3><strong>📑 Ready to Install and Run Samples</strong></h3><ul><li>Fully configured and factory tested before delivery</li><li>Full documentation set<ul><li>start-up and troubleshooting guide</li><li>detailed flow and plumbing schematics</li><li>spare parts list</li><li>method and factory test results</li></ul></li></ul><h3><strong>⚙️ Built to maximize productivity and uptime</strong></h3><ul><li>Two-oven design: Temperature programmable GC Oven and independent isothermal valve oven can hold up to six packed columns for optimal column management</li><li>Features Instant Connect detector modules, increasing uptime with detector exchange in minutes</li><li>Optional third isothermal oven for molecular sieve columns</li><li>Spacious independent valve oven with easy access to valves, rotors, and actuators</li><li>Heated sample bulkheads ensure complete sample transfer</li><li>Optimized system footprint to reduce bench space requirement</li></ul><h4><strong>🧪 Permanent Gas - CO/CO<sub>2</sub></strong></h4><ul><li>Single channel system for N<sub>2</sub>, O<sub>2</sub>, CO, and CO<sub>2</sub></li><li>Single channel for low level CO and CO<sub>2</sub></li><li>Dual channel system for N<sub>2</sub>, O<sub>2</sub>, and low-level CO/CO<sub>2</sub></li><li>Dual Channel system for N<sub>2</sub>, O<sub>2</sub>, CO, CO<sub>2</sub> and H<sub>2</sub></li></ul><h4><strong>🧪 Trace-level impurities at ppb-ppt in pure gases</strong></h4><ul><li>Impurities in Oxygen - H<sub>2</sub>, Ar, N<sub>2</sub>, CO, CO<sub>2</sub> and C1-C4</li><li>Impurities in Helium - H<sub>2</sub>, Ar, N<sub>2</sub>, O<sub>2</sub>, CO, CO<sub>2</sub> and C1-C4</li><li>Impurities in inert, pure gases - H<sub>2</sub>, Ar, N<sub>2</sub>, O<sub>2</sub>, CO, CO<sub>2</sub> and C1-C4</li></ul><h4><strong>🧪 Impurities in Chlorine Gas</strong></h4><ul><li>Channel 1 - H<sub>2</sub></li><li>Channel 2 - Ar, N<sub>2</sub>, O<sub>2</sub>, CO, CO<sub>2</sub> and C1-C4</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/application::product.product/TRACEtm-1310-GC-Analyzers-for-Permanent-Gases-and-Trace-Impurities.jpg" alt="Thermo Scientific TRACE 1310 GC Analyzers for Permanent Gases &amp; Trace Impurities" width="null" height="null"></p></div></div>

Thermo Scientific TRACE 1310 GC Analyzers for Dissolved Gases

Automate analysis of dissolved gases in transformer oils with TRACE GC 1310. Fully compliant with ASTM D3612 A/C, pre-configured, tested, and ready for fast startup.

<p><strong>Automate the analysis of dissolved gases in transformer insulating oils using the Thermo Scientific™ TRACE™ GC for Dissoved Gases. This gas chromatography system is based on the robust and reliable Thermo Scientific™ TRACE™ 1310 series gas chromatograph.&nbsp;</strong></p><p>It includes all of the components required to measure gas samples from transformer oil and is fully compliant with ASTM D3612 Method A or C. The system is delivered pre-configured and tested to enable fast startup and commissioning. Documentation includes startup procedures, troubleshooting guide, instrument method, and recommended spare parts.</p><h3><strong>Ready to install and run samples</strong></h3><ul><li>Fully configured and factory tested</li><li>Designed to meet industry standard methods - <strong>ASTM 3612, Method A or C</strong></li><li>Includes method delivered with system</li><li>Includes commission and training upon installation</li><li><strong>Available in two configurations:&nbsp;</strong><ul><li><strong>TOGAMAC010011</strong> -Thermal Conductivity Detector (TCD) in series with methanizer Flame Ionization Detector (FID)</li><li><strong>TOGAMAC010021 </strong>- TCD in series with methanizer FID. Includes integrated bypass to allow diversion of sulfur species and sulfur hexafluoride to prevent damage to nickel catalyst.</li></ul></li><li>Enables analysis of samples from vacuum extraction via manual gas-tight syringe or automated headspace sampling systems</li><li>Features Instant Connect detector modules, which increase uptime by enabling detector exchange in minutes</li><li>Offers optional third isothermal oven for molecular sieve columns</li><li>Designed for increased uptime and ease of support</li><li>Features spacious independent valve oven with easy access to valves, rotors, and actuators</li><li>Optimized system footprint to reduce bench space requirement</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_TRACE_1310_GC_Analyzers_for_Dissolved_Gases_a826e9752a.jpg" alt="Thermo Scientific TRACE 1310 GC Analyzers for Dissolved Gases" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_TRACE_1310_GC_Analyzers_for_Dissolved_Gases_a826e9752a.jpg 245w," sizes="100vw" width="1021" height="594"></p>

Thermo Scientific™ TriPlus™ 100 LS Liquid Autosampler

Move to the simplicity of this X-Y-Z robotic autosampler. Able to accommodate most samples, the Thermo Scientific™ TriPlus™ 100 LS liquid autosampler completely and reliably automates liquid injections, from nanovolume to large sample volumes. 

<div><div id="product-description"><p>Move to the simplicity of this X-Y-Z robotic autosampler. Able to accommodate most samples, <strong>the Thermo Scientific™ TriPlus™ 100 LS liquid autosampler</strong> completely and reliably automates liquid injections, from <strong>nanovolume to large sample volumes.</strong> <strong>The precise movement control</strong> of the autosampler allows correct sampling and injection optimization even for <strong>highly viscous samples or very volatile solvents.</strong> <strong>Based on the robust and proven technology of the TriPlus RSH robotic autosampler, the TriPlus 100 LS increases liquid sample handling automation and throughput with ease.</strong></p></div><div><h3><strong>Labor saving</strong></h3><ul><li><strong>Extended unattended operations</strong> made possible by the wide sample storage and large solvent capacities</li><li><strong>High-Throughput Configuration</strong> is possible through a dual GC or GCMS set-up, with Double Pro or Confirmation injection mode</li><li>Rapid Mode for increased sample throughput starts <strong>the syringe washing cycle during the GC cooling phase</strong></li></ul><h3><strong>Versatilty</strong></h3><ul><li><strong>Select from different pre-optimized liquid injection modes </strong>for a wide range of applications</li><li><strong>Use with a full range of performance-enhancing accessories </strong>for liquid sample handling of the <strong>Thermo Scientific™ TriPlus™ RSH and RSH SMART platforms</strong> for additional simplicity and dependability</li><li><strong>Syringe Cleaning options</strong> allow to install multiple solvent stations and wxpand solvent and waste volumes</li><li><strong>Add module options </strong>including an agitator, vortexer, liquid-cooled tray holder, and Temperature-Controlled Drawer</li></ul><h3><strong>Easy operations</strong></h3><ul><li><strong>Eight fully customizable method-specific preset menus</strong> for different injection modes are available through the data systems to easily allow the highest flexibility in liquid samples injections</li><li><strong>Optional hand-held control device</strong> with 4 keys, round knob, and graphical LCD display for local control</li><li>Thermo Scientific chromatography data systems integrated with<strong> Virtual Terminal software </strong>to completely mimic the physical controller</li></ul><figure class="image"><img style="aspect-ratio:650/567;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Tri_Plus_100_LS_Liquid_Autosampler_3_0238d1dc37.jpg" alt="Thermo Scientific™ TriPlus™ 100 LS Liquid Autosampler" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Tri_Plus_100_LS_Liquid_Autosampler_3_0238d1dc37.jpg 179w," sizes="100vw" width="650" height="567"></figure><h3><strong>Specifications</strong></h3><ul><li><strong>Type:</strong> TriPlus 100 LS Liquid Autosampler</li><li><strong>Sample Capacity:</strong><ul><li>Up to 4608 well plates</li><li>Up to 840 0.5/0.7 mL vials</li><li>Up to 648 2 mL sample vials</li><li>Up to 180 10 mL or 20 mL vials</li></ul></li><li><strong>Injection Mode:</strong> 8 Fully Customizable Method-Specific Injection Mode Presets</li><li><strong>Injection Speed:</strong> Selectable from 0.1 µL/sec up to 900 µL/sec and fully programmable in three different steps, also dependent upon the volume of the installed syringe</li><li><strong>Injection Volume:</strong> 0.1 to 100.0 μL</li><li><strong>Description:</strong> Robotic XYZ Liquid Autosampler for GC and GCMS</li><li><strong>Tool Exchange:</strong> No tool exchange capability</li><li><strong>For Use With (Equipment):</strong> Compatible with standard and SMART syringes</li><li><strong>Quantity:</strong> Each</li><li><strong>Unit Size:</strong> Each</li></ul></div></div>

Thermo Scientific TRACE 1310 GC Greenhouse Gas Analyzers

TRACE 1310 GC Greenhouse Gas Analyzers are preconfigured and tested systems for automated GHG analysis, with optional autosampler and Chromeleon CDS for data processing.

<div><p><strong>Measure greenhouses gases and report on sample results with automated Thermo Scientific™ TRACE™ 1310 GC Greenhouse Gas Analyzers. Preconfigured and method tested, these systems make it easy to analyze your samples. Run one sample at a time or add a headspace autosampler to run multiple samples automatically.&nbsp;</strong></p><p>The <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/76"><strong>Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)</strong></a> provides a powerful software tool to acquire and analyze your GC data. Each system is delivered with methods pre-installed and a complete documentation package, including a start-up guide, troubleshooting tips, a plumbing diagram, and spare parts/consumables list for easy ordering.</p><div><h3><strong>⚙️&nbsp;Two systems to choose from:</strong></h3><h4><strong>Two-channel system with FID-ECD analysis</strong></h4><ul><li>FID channel &amp; methanizer detects methane (CH<sub>4</sub>) and CO<sub>2</sub> after converting to CH<sub>4</sub> in the methanizer</li><li>ECD detects nitrous oxide (N<sub>2</sub>O)</li></ul><h4><strong>Three Channel system with FID-ECD-TCD analysis</strong></h4><ul><li>FID Channel detects methane (CH<sub>4</sub>)</li><li>ECD Channel detects nitrous oxide (N<sub>2</sub>O)</li><li>TCD Channel detects carbon dioxide (CO<sub>2</sub>)</li></ul><h3><strong>🔧&nbsp;Built to maximize productivity and uptime</strong></h3><ul><li><strong>Two oven design</strong> - temperature programmable GC oven and independent isothermal valve oven can hold up to 6 packed columns for optimal column management</li><li>System features Instant Connect detector modules increasing uptime with detector exchange in minutes</li><li><strong>Spacious independent valve oven</strong> with easy access to valves, rotors, actuators</li><li>Heated sample bulkheads ensure complete sample transfer</li><li><strong>Optimized system footprint</strong> to reduce bench space requirement</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/application::product.product/Thermo-TRACEtm-1310-GC-Greenhouse-Gas-Analyzers.jpg" alt="Thermo Scientific TRACE 1310 GC Greenhouse Gas Analyzers" width="null" height="null"></p></div></div>

Thermo Scientific TRACE 1310 GC Refinery Gas Analyzers

TRACE 1310 Refinery Gas Analyzers: pre-tested, ready-to-use systems for ASTM, UOP, GPA, ISO methods. Fast RGA performance, Instant Connect, and full documentation.

<div><div><p><strong>Experience outstanding reliability and performance in your gas chromatography analyses with Thermo Scientific™ TRACE™ 1310 Refinery Gas. Analyzers. We offer a wide selection of configurations for ASTM D1945, ASTM D7833, UOP 539, ASTM 2163, GPA 2166/2286, and ISO 6974. Pre-configured and factory tested, all configurations of TRACE 1310 Refinery Gas Analyzers are ready to analyze your samples right out of the box.</strong>&nbsp;</p><p>Experience easier maintenance and greater uptime with patented Instant Connect modularity. We offer six models to select from based upon your sample requirements and all deliver 'Fast RGA' performance. Each system includes a start-up guide with troubleshooting tips and full plumbing diagrams.</p></div><div><h3><strong>✅ Ready to Install and Run Samples</strong></h3><ul><li>Fully configured and factory tested</li><li>Designed to meet industry standard methods - ASTM UOP ISO</li><li>Method delivered with system</li><li>Commission and training on installation</li></ul><h3><strong>🔧 Variety of configurations to meet your requirements</strong></h3><h4><strong>3-Three-channel configurations</strong></h4><ul><li><strong>Ch1: </strong>PG &amp; CO<sub>2</sub>, Ch2: C1-C8, Ch3: H<sub>2</sub>/He</li><li><strong>Ch1:</strong> PG &amp; CO<sub>2</sub>, Ch2: C1-C5 C6+, Ch:3 H<sub>2</sub>/He</li><li><strong>Ch1: </strong>PG &amp; CO<sub>2</sub> H<sub>2</sub>S, Ch2: C1-C5 C6+, Ch:3 H<sub>2</sub>/He</li></ul><h4><strong>3-Four-channel configurations for expanded capability</strong></h4><ul><li><strong>Ch1: </strong>PG &amp; CO<sub>2</sub>, Ch2: C1-C5 C6+, Ch3: C1-C8, Ch4: H<sub>2</sub>/He</li><li><strong>Ch1: </strong>PG &amp; CO<sub>2</sub>, Ch2: C1-C8, Ch:3 low level CO &amp; CO<sub>2</sub>, Ch:4 H<sub>2</sub>/He</li><li><strong>Ch1:</strong> PG &amp; CO<sub>2</sub>, Ch2: C1-C8, Ch:3 H<sub>2</sub>/He, Ch4: Sulfur speciation</li></ul><h3><strong>🧪 Built for speed and flexibility</strong></h3><ul><li>All systems are <strong>'fast RGA' designs</strong> for <strong>faster sample turnaround</strong></li><li><strong>Two-oven design</strong> – Temperature programmable GC Oven and <strong>independent isothermal valve oven</strong> can hold <strong>up to 6 columns</strong> for <strong>optimal column management</strong></li><li>System features <strong>Instant Connect detector modules</strong>, increasing <strong>uptime</strong> with <strong>detector exchange in minutes</strong></li><li><strong>Four independent detector signals</strong> for <strong>simultaneous data collection</strong>, simplified data reduction and troubleshooting</li><li>Optional <strong>third isothermal oven</strong> for <strong>molecular sieve columns</strong></li><li>Designed for <strong>increased uptime</strong> and <strong>ease of support</strong></li><li><strong>Spacious independent valve oven</strong> with <strong>easy access</strong> to valves, rotors, actuators</li><li><strong>Heated sample bulkheads</strong> ensure <strong>complete sample transfer</strong></li><li><strong>Optimized system footprint</strong> to <strong>reduce bench space requirement</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/application::product.product/Thermo-TRACEtm-1310-Refinery-Gas-Analyzers-1.jpg" alt="Thermo Scientific TRACE 1310 GC Refinery Gas Analyzers" width="null" height="null"></p></div></div>

Thermo Scientific TRACE 1310 GC Analyzers for Oxygenates

TRACE 1310 GC analyzers for oxygenates: preconfigured, tested systems for refinery streams. Automated, Chromeleon CDS-ready, with full documentation and ASTM methods.

<div><p><strong>Automatically analyze single or multiple samples using the Thermo Scientific™ TRACE™ 1310 Analyzers for Oxygenates. These automated laboratory gas chromatography systems measure and report oxygenates and octane enhancers in refinery product streams. Systems are preconfigured, method tested, and ready to analyze samples after installation.&nbsp;</strong></p><p>These systems can use the powerful <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/76"><strong>Thermo Scientific™ Chromeleon™ Chromatotraphy Data System (CDS)</strong></a> to acquire and analyze chromatography data. Custom reporting allows generation of customer-specific reports. Each system is delivered with methods pre-installed and a complete documentation package that includes start-up, troubleshooting, plumbing diagram, and spare parts/consumables list.</p><div><p>We offer three systems configured for specific hydrocarbon streams and ASTM standard test methods. All systems utilize automated backflush of stripper column to prevent contamination of sensitive oxygentates column thus increasing uptime and performance.</p><h4><strong>TRACE™ 1310 GC for Oxygenates by ASTM D7423</strong></h4><ul><li>FID Channel detects and quantitates oxygenate compounds in light hydrocarbon streams C2-C5 as specified in ASTM D7423. Stripper column holds hydrocarbons up for backflush to prevent contamination of oxygenates column.</li></ul><h4><strong>TRACE™ 1310 GC for Oxygenates by ASTM D7754</strong></h4><ul><li>FID Channel detects and quantitates oxygenate compounds in spark ignition fuels (gasoline) as specified in the ASTM D7754. Stripper column holds hydrocarbons which are vented by a pressure point tee to prevent contamination of oxygenates column.</li></ul><h4><strong>TRACE™ 1310 GC for Oxygenates in Gasoline by ASTM D4815</strong></h4><ul><li>FID Channel detects and quantitates oxygenate compounds in spark ignition fuels (gasoline) as specified in the ASTM D4815. Stripper column holds hydrocarbons which are vented by a pressure point Tee to prevent contamination of oxygenates column.</li></ul><h3><strong>Built to maximize productivity and uptime</strong></h3><ul><li>Systems features<strong> Instant Connect detector modules</strong> increasing uptime with detector exchange in minutes</li><li>Heated sample bulkheads ensures complete sample transfer</li><li><strong>Automated injection</strong> for liquid samples</li><li><strong>Optimized system footprint</strong> to reduce benchspace requirement</li></ul></div></div>

Products focused on GC distributed by Thermo Fisher Scientific (EMEA) - page 1

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