<p><strong>This study investigates the chemical transformations of Eugenia uniflora essential oil under thermal conditions simulating gas chromatography analysis. GC–MS and NMR revealed that sesquiterpenes of the germacrene type, such as furanodiene, undergo sigmatropic rearrangement to form elemene-type compounds, including curzerene.</strong></p><p>Thermal treatment confirmed the formation of rearrangement products, indicating that bioactive properties historically attributed to curzerene may instead originate from furanodiene. The findings highlight the importance of combining complementary analytical techniques, such as NMR, to avoid misinterpretation of essential oil composition and bioactivity markers.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966"><strong>Is Curzerene Responsible for the Bioactive Properties of Eugenia uniflora? A Possible Misinterpretation of Bioactive Markers</strong></a></h4><p>Vinicius Monteiro Schaffka, Raphaela Pereira Guaringue, Larissa Kozan, André Luis Kerek, Cássia Gonçalves Magalhães, Andersson Barison, and Barbara Celânia Fiorin*</p><p>ACS Omega 2025, 10, 48, 59425–59434</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acsomega.5c08966">https://doi.org/10.1021/acsomega.5c08966</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><div><p>Essential oils are a matrix of volatile and complex compounds characterized by strong odors and significant biological activity. In plants, they play a crucial role in defense against bacteria, viruses, and predators. <a href="javascript:void(0);">(1)</a> These biological properties support the extensive use of essential oils in the food, cosmetics, and pharmaceutical industries. <a href="javascript:void(0);">(1,2)</a> <i>Eugenia uniflora</i> L., commonly known as pitangueira, is a native Brazilian tree widely distributed across South America and is well-known for its bioactive essential oil. <a href="javascript:void(0);">(3)</a> Beyond the consumption of its fruits in natura or functional beverages, its leaves are used in infusions, decoctions, and tinctures to treat a variety of diseases, including diarrhea, stomach pain, worm infestations, fever, flu, hyperglycemia, hyperlipidemia, and hypertension. <a href="javascript:void(0);">(4,5)</a> Studies have shown that the composition of secondary metabolites in <i>E. uniflora</i> can vary depending on factors such as fruit color, ripening stage, <a href="javascript:void(0);">(6)</a> and seasonal influences. <a href="javascript:void(0);">(4,7−10)</a></p><p>The essential oil of <i>E. uniflora</i> is predominantly composed of sesquiterpenes, mainly germacrene-type, such as curzerene, germacrene B, germacrone, germacrene D, selina-1,3,7(11)-trien-8-one, and its oxide. Additionally, Elemene-type terpenes are also reported, including furanodiene, β-Elemene, and β-Elemenone. <a href="javascript:void(0);">(11−13)</a></p><p>However, those sesquiterpenes are well-documented for their thermal instability. When heat is applied, germacrene-type sesquiterpenes undergo a [3,3]-sigmatropic rearrangement, known as Cope rearrangement, converting into Elemene-type terpenes as presented in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966#fig1">Figure 1</a>. <a href="javascript:void(0);">(14−16)</a> This reaction is widely utilized as a synthetic pathway to produce Elemenes from germacrene precursors. <a href="javascript:void(0);">(17,18)</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_48_59425_59434_Figure_1_3_3_Sigmatropic_rearrangement_of_Germacrene_type_into_Elemene_type_250708e73c.jpg" alt="ACS Omega 2025, 10, 48, 59425–59434: Figure 1. [3,3]-Sigmatropic rearrangement of Germacrene-type into Elemene-type." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_48_59425_59434_Figure_1_3_3_Sigmatropic_rearrangement_of_Germacrene_type_into_Elemene_type_250708e73c.jpg 245w," sizes="100vw" width="3000" height="984"></p><p>Gas chromatography (GC) is the main technique for characterizing essential oils, often coupled with mass spectrometry (GC-MS) and the retention index determination is used in the identification of the compounds through comparison with databases. <a href="javascript:void(0);">(21)</a> During GC analysis, essential oils are subjected to multiple heating stages. Initially, the sample is exposed to a high inlet temperature, typically around 240 °C, to ensure complete volatilization. It then enters the oven, where the temperature follows a programmed ramp starting at 60 °C, increasing at 3 °C/min, and reaching up to 240 °C. Finally, the sample encounters the elevated temperatures of the mass spectrometry (MS) detector’s source (250 °C) and interface (280 °C). Even in isocratic methods, where the oven temperature remains constant throughout the analysis, the inlet, source, and interface temperatures are maintained at high levels. Although the sample passes through these stages briefly, the elevated temperatures can induce Cope rearrangements in the essential oil, leading to possible changes in its composition. <a href="javascript:void(0);">(22,23)</a></p><p>The accurate characterization of the chemical composition of essential oils is crucial for any study employing them as a biological or analytical matrix, as it supports the interpretation of bioactivity, chemotaxonomic classification, and quality control of samples with economic value added. There are many studies attributing relevant biological activities, classifying specimens, and identifying phenotypic and genetic variations based on the chemical composition of the essential oil of <i>E. uniflora</i> L., which is usually characterized by GC-MS. <a href="javascript:void(0);">(8,9,11,24)</a> As an example of the study carried out by de Jesus, found acute anti-inflammatory effect of a rich in curzerene essential oil. <a href="javascript:void(0);">(5)</a> Similarly, Pascoal, reported distinct essential oil composition patterns among <i>E. uniflora</i> varieties─yellow, red, and purple fruits─primarily driven by variations in β-Elemene and germacrene D content. <a href="javascript:void(0);">(7)</a> These findings highlight metabolic changes during fruit ripening and underscore the influence of genetic diversity among <i>E. uniflora</i> varieties. Such exhibitions are unsatisfactory, because they sustain all their evidence in the characterization of their essential oil only by GC-MS analysis, without considering the Cope rearrangements, a fact that may change the outcome of their research. In this context Nuclear Magnetic Resonance (NMR) spectroscopy is a good alternative for essential oil analysis, since is a robust not destructive technique and able to detect and quantify simultaneously a wide range of metabolites that exceed the concentration of about 5–10 μM. <a href="javascript:void(0);">(25)</a></p><p>In order to highlight analytical concerns about the uses of only GC-MS data to access chemical relevance of <i>E. uniflora</i> essential oil studies, Nuclear Magnetic Resonance (NMR) spectroscopy was employed to evaluate the chemical composition of the essential oil of <i>Eugenia uniflora</i> L. before and after an induced thermal treatment, compared to GC-MS data. The findings provide insights into thermolabile compounds and highlight potential misidentifications of the bioactive properties associated with the sesquiterpenes of <i>E. uniflora</i> present in literature.</p><h4><strong>2. Results and Discussion</strong></h4><h5><strong>2.1. Characterization of the Essential Oil</strong></h5><p>Approximately 60 compounds, mostly sesquiterpenes (Germacrene B) and oxygenated sesquiterpenes (Germacrone), were visualized in the essential oil, with the 9 compounds having a relative area above 2%, as shown in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966#tbl1">Table 1</a>. The identified and most prevalent compounds align with those reported in the literature for <i>E. uniflora</i>. <a href="javascript:void(0);">(4,11,12)</a> It is possible to highlight the significant proportion of Germacrene-type sesquiterpenes compared to Elemene-type, except Curzerene, which was found in high concentration.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_48_59425_59434_Table_1_Main_Components_Identified_in_the_GC_MS_Analysis_of_the_Essential_Oil_from_Leaves_of_E_uniflora_9d59d96bef.jpg" alt="ACS Omega 2025, 10, 48, 59425–59434: Table 1. Main Components Identified in the GC–MS Analysis of the Essential Oil from Leaves of E. uniflora." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_48_59425_59434_Table_1_Main_Components_Identified_in_the_GC_MS_Analysis_of_the_Essential_Oil_from_Leaves_of_E_uniflora_9d59d96bef.jpg 218w," sizes="100vw" width="1076" height="771"></p><p>The variation in the composition of the essential oil from <i>E. uniflora</i> leaves was described in literature, <a href="javascript:void(0);">(9)</a> who classified the specimens into three groups: Group I, containing yellow, dark red, and purple fruits, which showed high concentrations of germacrene B, germacrone, and atractylone; Group II, consisting of light red fruit samples, with high concentrations of curzerene, germacrene D, and germacrene A; and Group III, containing orange-red fruits, with a high content of selina-1,3,7(11)-trien-8-one and oxyselina-1,3,7(11)-trien-8-one. According to this classification, this essential oil fits into Group II.</p><p>Chemical transformations of many components found in the <i>E. uniflora</i> leaves essential oil result from the heating conditions typically applied during GC–MS analysis, which occur through Cope Rearrangement. <a href="javascript:void(0);">(12,19)</a> Cope rearrangement is a stereospecific [3,3]-sigmatropic rearrangement that proceeds via the most stable chairlike transition state of 1,5-cyclodecadiene, from germacrene-type, to give 1,2-divinylcyclohexane, known as Elemene-type. <a href="javascript:void(0);">(14)</a> Some of the Cope rearrangements found in the <i>E. uniflora</i> essential oil are represented in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966#fig2">Figure 2</a>. <a href="javascript:void(0);">(11,22,23,26)</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_48_59425_59434_Figure_2_Cope_Rearrangements_of_sesquiterpenes_7fe9282d75.jpg" alt="ACS Omega 2025, 10, 48, 59425–59434: Figure 2. Cope Rearrangements of sesquiterpenes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_48_59425_59434_Figure_2_Cope_Rearrangements_of_sesquiterpenes_7fe9282d75.jpg 150w," sizes="100vw" width="1500" height="1566"></p><p>The same essential oil was analyzed using NMR spectroscopy to identify its components. Although NMR identification is considerably more labor-intensive than GC-MS analysis, it offers the advantage of being a cold and nondestructive technique. Since the sample does not undergo heating during the process, its stability is preserved throughout the analysis. The conformational differences observed in germacrene-type compounds further increase the spectrum’s complexity, leading to signal broadening in the <sup>1</sup>H NMR spectra. <a href="javascript:void(0);">(27)</a></p><p>A substantial number of signals were observed in the <sup>1</sup>H spectrum, with the majority concentrated between 0.5 and 3.0 ppm, a region characteristic of terpene compounds. The region between 4.5 and 6.0 ppm also exhibited numerous signals attributed to vinylic protons, observed in sesquiterpenes (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.5c08966#fig3">Figure 3</a>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2025_10_48_59425_59434_Figure_3_1_H_NMR_400_13_M_Hz_CD_Cl3_spectra_of_the_raw_essential_oil_a46e04574e.jpg" alt="ACS Omega 2025, 10, 48, 59425–59434: Figure 3. 1H NMR (400.13 MHz, CDCl3) spectra of the raw essential oil." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2025_10_48_59425_59434_Figure_3_1_H_NMR_400_13_M_Hz_CD_Cl3_spectra_of_the_raw_essential_oil_a46e04574e.jpg 245w," sizes="100vw" width="4175" height="2376"></p><h4><strong>3. Conclusions</strong></h4><p>The investigation of the composition of <i>E. uniflora</i> essential oil by NMR and the thermal treatment performed proved that curzerene may come from furanodiene due to the transformations that occur in the matrix during gas chromatographic analysis. These findings have significant implications for the investigation into the chemical composition of <i>E. uniflora</i> essential oil and the evaluation of the biological properties of its sesquiterpenes. Since each technique has advantages and disadvantages, combining GC-MS analysis with a cold technique, such as NMR is important to guarantee the proper identification of the compound genuinely responsible for any property assigned to <i>E. uniflora</i> essential oil.</p><h4><strong>4. Materials and Methods</strong></h4><h5><strong>4.2. GC-MS Analysis</strong></h5><p>The gas chromatographic analyses of the essential oils (10 mg mL<sup>–1</sup>) were performed using a gas chromatography–mass spectrometry system (<a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=1&amp;search=QP2020"><strong>Shimadzu GCMS-QP2020 Gas Chromatograph</strong></a>). The analyses were conducted on an RTx-5MS capillary column (30 m × 0.25 mm internal diameter × 0.25 μm film thickness), and the analytical conditions were as follows: split ratio of 1/10, injector temperature at 250 °C, ion source at 250 °C, and interface at 280 °C. The temperature program of the oven was set at 60 °C for 5 min, followed by a temperature ramp of 3 °C/min until reaching the final temperature of 240 °C. The components were identified based on the relative retention index, calculated for each constituent by injecting a series of <i>n</i>-alkane standards (C8–C20) under the same sample conditions and comparing them with tabulated values as well as by comparing the obtained mass spectra with the mass spectra database and literature comparisons. <a href="javascript:void(0);">(21)</a></p><h5><strong>4.4. NMR Analysis</strong></h5><p>All NMR spectra were acquired at 298 K on a <strong>Bruker AVANCE III</strong> spectrometer operating at 9.4 T, observing <sup>1</sup>H at 400.13 MHz and <sup>13</sup>C at 100.13 MHz, equipped with a broadband probe. The essential oil was solubilized in CDCl<sub>3</sub> with 0.05% of tetramethylsilane (TMS) used as an internal reference. The <sup>1</sup>H NMR spectra were recorded utilizing 65k time-domain data points across a spectral width of 20.00 ppm. This setup provided a digital resolution of 0.24 Hz. The data were obtained using a single 30° excitation pulse, with a relaxation delay of 1.0 s, and an average of 128 scans. The <sup>13</sup>C NMR spectra was carried out using the <i>zgpg30</i> pulse sequence utilizing 64k time-domain data points across a spectral width of 238 ppm, with an acquisition time of 1.3631 s, providing a digital resolution of 0.7434 Hz and a relaxation delay of 2.0s and an average of 2k scans.</p><p>The HSQC and HMBC experiments were conducted using standard pulse sequences from the Bruker library, with modifications to the number of acquired points to improve resolution, 2048 in F2 and 512 in F1.</p></div>

Is Curzerene Responsible for the Bioactive Properties of Eugenia uniflora? A Possible Misinterpretation of Bioactive Markers

Quantitative Assessment of Chemical Modifications of Macromolecules through Counterion Measurement

<p><strong>In the week of March 30, the following webinars await you in the field of gas / solid phase and especially Raman, FTIR, GC and GC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><div><div><div><div><div><div><h5><strong>1. Agilent Technologies: Trace the Threat: Py-GC/MS Workflows for Microplastics Characterization</strong></h5><ul><li>Tu, 31.3.2026 06:30 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/6025">Registration</a></li></ul><p>Discover advanced approaches for trace micro- and nanoplastics analysis using Py-GC/MS and explore how the Agilent 5977C GC/MSD enhances sensitivity, robustness, and workflow efficiency.</p><h5><strong>2. Thermo Fisher Scientific: FTIR microscopy: Practical guidance for effective sample analysis</strong></h5><ul><li>Tu, 31.3.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/6088">Registration</a></li></ul><p>Join this webinar to gain practical guidance on FTIR microscopy workflows, including measurement modes, sample preparation, parameter optimization and spectral interpretation.</p><h5><strong>3. Bruker: Understanding Polymer Reactions and Degradation with FT-IR and EPR Spectroscopy</strong></h5><ul><li>Tu, 31.3.2026 17:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/5996">Registration</a></li></ul><p>Discover how FT-IR and EPR spectroscopy reveal polymer curing kinetics and radical-driven mechanisms—enabling real-time monitoring, degree of cure quantification, and stability studies.</p><h5><strong>4. Technology Networks: Advances in Hydrogen Gas Generation for Sustainable Laboratories</strong></h5><ul><li>Tu, 31.3.2026 17:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/5941">Registration</a></li></ul><p>Discover how modern hydrogen gas generators support high-purity GC performance while reducing costs and environmental impact. Learn how on-site generation improves uptime, efficiency, and lab sustainability.</p><h5><strong>5. C&amp;EN: Overcoming Raman Microscopy Challenges in Microplastics and Life Sciences</strong></h5><ul><li>We, 1.4.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/6016">Registration</a></li></ul><p>Learn how O-PTIR spectroscopy overcomes fluorescence limitations in Raman microscopy. Discover simultaneous sub-micron IR and Raman analysis for microplastics, biological samples, and organic materials.</p></div></div></div></div></div></div>

Webinars LabRulezGCMS Week 14/2026

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Waters Corporation

<div><h4><strong>Navigating Challenges and Opportunities for Environmental Testing Labs Under the Revised EU Water Framework Directive</strong></h4><p><strong>The European Commission’s proposed updates to the Water Framework, Groundwater, and Environmental Quality Standards Directives are bringing new responsibilities and opportunities for environmental testing labs. With expanded pollutant lists - including PFAS, pharmaceuticals, and pesticide metabolites - and stricter monitoring and reporting requirements, the landscape of water analysis is rapidly evolving.</strong></p><p>Join the 4th Waters Environmental Webinar Series to explore how your lab can navigate these challenges and turn regulatory change into opportunity.</p><p>Register for free and partner with us to shape the future of European water quality.</p><h5><strong>Why Attend</strong></h5><p>Stay ahead of new EU regulatory demands with insights from leading experts. Gain practical guidance, share experiences with peers, and prepare your lab for the next generation of water quality standards.</p><h5><strong>Highlights</strong></h5><ul><li>New pollutant categories and analytical challenges</li><li>Updates to monitoring protocols and reporting</li><li>Innovative methods to meet revised Environmental Quality Standards</li><li>Strategic guidance for compliance and adaptation</li></ul><p><strong>Lead the change in water analysis—stay ahead, stay compliant, and make a real impact.</strong></p><h4><strong>EPISODE 2: PFAS in the Environment: A Deep Dive into Analysis, Regulation, and Real-World Data</strong></h4><h4><strong>What you Will Learn</strong></h4><ul><li>How to maintain sub-ng/L LOQs in water samples with direct injection</li><li>How an extended number of PFAS and Relevant Potency Factors in the upcoming EU Water Framework Directive can be addressed</li></ul><blockquote><p><strong>Presenter:</strong> Jana Kováčová (PFAS Lead for Continental Europe, ALS Czech Republic)</p><p>Jana Kováčová is an analytical chemist with over 15 years of experience in method development and validation. She earned her MSc and PhD from the University of Chemistry and Technology, Prague (UCT Prague), where she specialized in analytical methods for pesticide residue determination in water and food matrices of both plant and animal origin.</p><p>In 2020, Jana joined ALS Czech Republic as a Validation Specialist within the environmental analytical innovations group, later transitioning to a full-time focus on PFAS (per- and polyfluoroalkyl substances) analytics. In 2022, she was appointed Supervisor of the PFAS Section.</p><p>Currently, Jana serves as the PFAS Lead for Continental Europe. In this role, she coordinates ALS’s PFAS analytical activities across the region, acting as a key technical representative for PFAS topics. She supports laboratories and clients with strategic guidance and data interpretation, aligns testing approaches across multiple sites, and ensures consistent, high-quality delivery. To stay at the forefront of the field, Jana closely monitors legislative updates and emerging trends, sharing her expertise through client webinars, seminars, and presentations at scientific conferences</p><p><strong>Presenter: </strong>Sara Beverly (Principal Sales Application Chemist, Waters)</p><p>In her current position, Sara is a Principal Sales Application Chemist specializing in food and environmental LC-MS/MS based applications within the Waters UK and Ireland demo laboratory. Sara has worked in F&amp;E analysis with various research interests including the development of both multi-residue HRMS screening and targeted LC/GC MS based methods for the past 25 years in both the government and private sectors. Prior to joining Waters Sara was employed as a Lead Scientist at the Food and Environment Research Agency (FERA, Defra, UK) for 14 years. Sara has participated in EU Food and Veterinary Office (FVO) audit inspections, was an expert on behalf of the International Atomic Energy Agency (IAEA) and a member of the EuroResidue conference scientific committee.</p><p><strong>Presenter: </strong>Claudia Rathmann (Principal F&amp;E Market Development Manager, Waters Corporation)</p><p>Experienced and passionate analytical science professional with over 20 years of technical and strategic expertise in food and environmental applications. Currently serving as Principal Market Development Manager at Waters Corporation, Claudia specializes in connecting customers with product management to deliver innovative solutions for complex analytical challenges. With a strong scientific foundation and a proven track record in both technical and marketing roles, Claudia bridges the gap between customer needs and product innovation across the EMEA region.</p></blockquote></div>

PFAS in the Environment: A Deep Dive into Analysis, Regulation, and Real-World Data

Explore PFAS analysis, regulation, and real-world data under the revised EU Water Framework Directive in Episode 2 of the Waters Environmental Webinar Series.

PFAS in Environment: Analysis, Regulation

<div><h4><strong>Navigating Challenges and Opportunities for Environmental Testing Labs Under the Revised EU Water Framework Directive</strong></h4><p><strong>The European Commission’s proposed updates to the Water Framework, Groundwater, and Environmental Quality Standards Directives are bringing new responsibilities and opportunities for environmental testing labs. With expanded pollutant lists - including PFAS, pharmaceuticals, and pesticide metabolites - and stricter monitoring and reporting requirements, the landscape of water analysis is rapidly evolving.</strong></p><p>Join the 4th Waters Environmental Webinar Series to explore how your lab can navigate these challenges and turn regulatory change into opportunity.</p><p>Register for free and partner with us to shape the future of European water quality.</p><h5><strong>Why Attend</strong></h5><p>Stay ahead of new EU regulatory demands with insights from leading experts. Gain practical guidance, share experiences with peers, and prepare your lab for the next generation of water quality standards.</p><h5><strong>Highlights</strong></h5><ul><li>New pollutant categories and analytical challenges</li><li>Updates to monitoring protocols and reporting</li><li>Innovative methods to meet revised Environmental Quality Standards</li><li>Strategic guidance for compliance and adaptation</li></ul><p><strong>Lead the change in water analysis—stay ahead, stay compliant, and make a real impact.</strong></p><h4><strong>EPISODE 6: Blue Thursdays 2026 – The Best Of</strong></h4><p>In this final session, we’ll look back at the most important things we’ve learned throughout the Blue Thursday series. We’ll explore the big trends we’re seeing, the smart tools scientists use to study the environment, and what exciting developments will be coming next.</p><blockquote><p><strong>Speaker:</strong> Claudia Rathmann (Principal F&amp;E Market Development Manager, Waters Corporation)</p><p>Experienced and passionate analytical science professional with over 20 years of technical and strategic expertise in food and environmental applications. Currently serving as Principal Market Development Manager at Waters Corporation, Claudia specializes in connecting customers with product management to deliver innovative solutions for complex analytical challenges. With a strong scientific foundation and a proven track record in both technical and marketing roles, Claudia bridges the gap between customer needs and product innovation across the EMEA region.</p><p><strong>Speaker: </strong>Janitha De-Alwis (JD) (Principal F&amp;E Market Development Manager, Waters Corporation)</p><p>Janitha De-Alwis (known as JD) is an applications chemist in the European demo lab, based in Wilmslow, UK. His main role focuses on application development work for the food and environmental sector. JD takes great pride in speaking to, and advising, many food and environmental customers. His position requires him to be reactive and proactive to the changes in the food industry, such as changing regulations, topical issues and food safety risks.</p></blockquote></div>

Blue Thursdays 2026 – The Best Of

In this final session, we’ll look back at the most important things we’ve learned throughout the Blue Thursday series.

<div><p><strong>Discover how your laboratory can stay ahead in microplastics analysis with this webinar which demystifies the ISO/FDIS 16094-2 standard, guiding you through best practices for vibrational spectroscopy in water testing. The standard highlights the regulatory push to monitor microplastics, the challenges in their detection due to varied sizes and polymer types, and the importance of reliable, standardized analytical techniques.</strong></p><p>Ultimately, the adoption of standardized procedures and advanced technologies is shown to enhance the reliability and comparability of microplastics data, supporting regulatory and scientific efforts to address environmental pollution in water resources.</p><h4><strong>Key Learning Objectives:</strong></h4><ul><li>Uncover best practices for vibrational spectroscopy when testing microplastics in water</li><li>Explore method validation, reporting, and quality control and their impact on laboratory operations</li><li>Emphasize the need for laboratories to verify measurement accuracy and establish reporting limits</li></ul><h4><strong>Who Should Attend:</strong></h4><ul><li>Microplastics researchers</li><li>Microplastics analysts from commercial, QA, or research labs seeking to understand key challenges in Microplastics analysis</li><li>Those interested in microplastics analysis and the development of standardized methodology around the world.</li></ul><blockquote><p><strong>Presenter:</strong> Darren Robey (Product Manager: IR Imaging, Agilent Technologies)</p></blockquote></div>

C&EN: Microplastic Analysis in Water Using Vibrational Spectroscopy: Navigating ISO/FDIS 16094-2 standards

Microplastic Analysis in Water Using Vibrational Spectroscopy: Navigating ISO/FDIS 16094-2 standards

Webinar on ISO/FDIS 16094-2 for microplastics in water, covering vibrational spectroscopy best practices, validation, QC, and standardized reporting.

Microplastic Analysis in Water by Vibrational Spectroscopy

<div><p><strong>Join the growing community of Andrew+ laboratory automation users and discover real research, practical insights, and peer‑to‑peer knowledge sharing.</strong></p><h4><strong>WHAT WILL YOU LEARN</strong></h4><ul><li>Real-world case studies from academic labs using Andrew+ today</li><li>Best-practice approaches shared directly by experienced users</li><li>Tips for integrating Andrew+ into your sample preparation workflows</li><li>Ways to automate tasks to increase efficiency and reduce variability</li><li>Opportunities to connect with peers facing similar research challenges</li></ul><h4><strong>WHO SHOULD ATTEND</strong></h4><ul><li>Academic researchers using or evaluating Andrew+</li><li>Lab members involved in sample preparation or workflow optimization</li><li>Anyone looking to introduce automation into their daily lab activities</li></ul><p><strong>REGISTER NOW!</strong></p><p>This event offers a unique chance to hear directly from the academic user community and discover practical insights that you can immediately apply in your own lab.</p><p>You’ll leave with new ideas, shared experiences, and a clearer understanding of how Andrew+ can support and streamline your research.</p><blockquote><p><strong>Presenter:</strong> Alex Dickens, Turku University</p><p><strong>Presenter: </strong>Otto Kauko, Turku University</p><p><strong>Presenter: </strong>Samira Salihovic, Örebro University</p><p><strong>Chairman: </strong>Paul Heads, Nordics Automation Specialist - Waters</p></blockquote></div>

Waters Corporation: Nordics Lab Automation Online Users Meeting

Nordics Lab Automation Online Users Meeting

Join the growing community of Andrew+ laboratory automation users and discover real research, practical insights, and peer‑to‑peer knowledge sharing.

<p><strong>The end-to-end sample journey represents a structured and traceable pathway that ensures every sample is managed with accuracy, integrity, and compliance from the moment an analysis request is raised to the secure archival of data. This comprehensive approach enables laboratories to deliver reliable results while maintaining operational efficiency and regulatory confidence.</strong></p><h4><strong>Why is this webinar a must‑attend?</strong></h4><p>Join us for this insightful webinar where you’ll explore essential elements of the Digital &amp; Automated Lab, including:</p><ul><li>Efficient management of analysis requests and sample registration, integrated with inventory management.</li><li>Streamlined processes for sample receipt and verification.</li><li>Automation in sample preparation and end‑to‑end sample tracking within chromatography workflows.</li><li>Comprehensive data handling from acquisition and review to reporting, storage, and archiving.</li></ul><p>By overseeing the sample journey from the initial analysis request through to final archival, you ensure complete traceability, stronger data integrity, and improved operational performance. This structured lifecycle not only reinforces compliance and quality standards but also builds stakeholder confidence by guaranteeing that every sample is handled with accuracy and accountability.</p><p>Register now and learn how to turn your scattered digital lab workflows into a connected, holistic, and automated lab ecosystem.</p><blockquote><p><strong>Presenter: </strong>Dr. Nirmal Thakker (Technical Application Support Specialist - Chromatography, Dissolution &amp; Lab Informatics, Agilent Technologies)</p><p>Dr. Nirmal has done his PhD in quality assurance and pharmacy and his master's in pharmacy from the University of Pune. He has more than 15 years of overall industrial experience working in analytical research &amp; development for renowned multinational pharma companies like Glenmark, Torrent, and Piramal. He has also worked with Spinco Biotech as an application specialist for LC. Presently, he is working with Agilent Technologies as a technical application support specialist for chromatography, dissolution, and lab informatics. He has expertise in chromatography, QbD, method transfer, and mass spectrometry.</p><p><strong>Presenter: </strong>Mr. Amit Ghate (Application Specialist - Lab Informatics, Agilent Technologies)</p><p>Amit Ghate is currently working as Lab Informatics Application Specialist at Agilent Technologies. Amit comes from analytical software background where he has an in-depth knowledge of variety of software like Chromatography Data Systems, Data Management, LIMS, Electronic Lab Notebook, databases, reports and Compliance. Prior to joining Agilent, Amit worked with Persistent Systems Ltd as a Project Manager for software support and services. He has done his Masters in Bioinformatics from University of Pune. His main areas of interest is in providing workflow solutions and enabling digital transformation of labs.</p></blockquote>

Agilent Technologies: Automation at the Next Level: Never Lose Track of Your Sample

Automation at the Next Level: Never Lose Track of Your Sample

Webinar on digital lab automation, covering end-to-end sample tracking, chromatography workflows, data integrity, and compliance.

Automation: Never Lose Track of Your Sample

<p><strong>Anti-doping control laboratories operate within a highly competitive field, where efficiency plays a key role. In this webinar, we’re proud to feature two experts and share their insights with you.</strong></p><p>Dr. Aðalheiður Dóra Albertsdóttir and Wim van Gansbeke will be providing a window into the operations of a lab that analyses up to 16.000 samples per year with only 21 staff members.</p><p>A method for quantifying endogenous anabolic androgenic steroids in urine using hydrogen as the carrier gas has been further optimized through the implementation of a Deans switch. In this configuration, the column flow is directed to the MS only during the elution of the target compounds.</p><p>Join us and explore lowering costs and maintenance with hydrogen carrier gas and Deans switch technology in steroid analysis.</p><blockquote><p><strong>Presenter: </strong>Dr. Aðalheiður Dóra Albertsdóttir (Deputy Head of the GC-MS Department, DoCoLab, University of Gent, Belgium)</p><p><strong>Presenter:&nbsp;</strong>Wim van Gansbeke (Head of the GC-MS Department, DoCoLab, University of Gent, Belgium)</p></blockquote>

Agilent Technologies: Cost And Maintenance Reduction Through The Use Of Hydrogen Carrier Gas And The Deans Switch When Analysing Silylated Steroids

Cost And Maintenance Reduction Through The Use Of Hydrogen Carrier Gas And The Deans Switch When Analysing Silylated Steroids

Join us and explore lowering costs and maintenance with hydrogen carrier gas and Deans switch technology in steroid analysis.

Cost & Maintenance Reduction by Use Of Hydrogen Carrier Gas

<div><h3><strong>Decoding Metabolic Responses in Complex Biology</strong></h3><p><strong>Join us for our latest webinar introducing ecTOF for life sciences applications. This next‑generation GC‑MS platform is designed to expand metabolite coverage and improve unknown annotation in complex samples.</strong></p><p>Most GC‑MS systems force you to pick between EI (great fragmentation, sometimes ambiguous IDs) and CI (better molecular ion, complementary information). The ecTOF acquires EI and CI data simultaneously in a single analysis, giving you more mass spectrometric evidence for each compound without extra runs or compromises.</p><h4><strong>What this means for your lab:</strong></h4><ul><li><strong>Detect more metabolites</strong> including those missed by EI‑only</li><li><strong>Increase confidence</strong> in spectral library matches and annotations</li><li><strong>Reduce false positives</strong> and speed up unknown triage</li><li><strong>Boost throughput</strong> with richer data per injection</li></ul><p>In this webinar, our esteemed speakers Dr. Volker Behrends and Dr. Sonja Klee will show how <strong>GC-MS-based profiling including novel ecTOF-based analysis was applied to assess the impact of MRSA infection on host central carbon metabolism</strong> with or without treatment.</p><p>We’ll walk through how GC-MS and specifically the ecTOF helps characterize metabolite shifts in a challenging cell model (A549 cells infected with S. aureus USA300 LAC and treated with different drugs), showing how EI+CI improves identification confidence and expands the detectable chemical space. The same principles and workflows apply broadly to host–pathogen studies, drug metabolism, toxicology, and systems biology.</p><p><strong>Discover how in even complex samples, ecTOF’s parallel EI+CI data enables clearer annotations and deeper biological interpretation.</strong></p><h4><strong>Key Learning Objectives</strong></h4><ul><li>How dual EI+CI in one run increases metabolite coverage and improves unknown identification</li><li>Practical ways to reduce ambiguity in spectral matching and raise confidence in annotations</li><li>How ecTOF integrates with existing GC methods, with no need to choose between EI or CI</li><li>Real‑world results from complex biological samples, with takeaways that translate to any GC‑MS metabolomics workflow</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>Life science researchers investigating complex molecular mechanisms in biological context</li><li>Metabolomics researchers expanding beyond targeted panel measurements</li><li>Exposomics and environmental health scientists mapping biological responses to broad and potentially undiscovered chemical stimuli.</li><li>Toxicology, food, and fragrance labs seeking broader chemical coverage</li><li>Core facilities &amp; omics method developers modernizing GC‑MS capability</li><li>LC‑MS users who want complementary coverage for better coverage of the metabolome</li></ul><blockquote><p><strong>Presenter:</strong> Dr. Volker Behrends, Ph.D., Associate Professor of Microbial Metabolism, School of Medicine and Biosciences, University of West London, London, UK</p><p>Dr. Volker Behrends is Associate Professor of Microbial Metabolism in the School of Medicine and Biosciences at the University of West London. His research focuses on how bacterial pathogens remodel host environments, using GC-MS–based metabolomics to track metabolite dynamics during infection. His group integrates microbial physiology with quantitative metabolomics to map metabolic fluxes and investigate how repurposed drugs alter pathogen behavior and host–pathogen interactions.</p><p><strong>Presenter: </strong>Dr. Sonja Klee, Product Manager ecTOF, TOFWERK, Thun, Switzerland</p><p>Dr .Sonja Klee is the product manager for the ecTOF platform at TOFWERK AG in Thun, Switzerland. She obtained her Ph.D. in physical chemistry from the University of Wuppertal. She was awarded a Wolfgang Pauli Study Award from the DGMS and was a Marie Curie Fellow at the CLOUD TRAIN network at CERN. She is a board member of the Swiss Group Mass Spectrometry (DGMS) and a member of the steering committee for BP4NTA (BestPractice4NonTargetAnalysis) working group.</p></blockquote></div>

Bruker: ecTOF: Advancing Metabolomics with Next‑Generation GC‑HRMS and Dual EI+CI Insight

ecTOF: Advancing Metabolomics with Next‑Generation GC‑HRMS and Dual EI+CI Insight

Discover how simultaneous EI + CI acquisition on ecTOF unlocks metabolome coverage and more annotation for untargeted and semi-targeted GC‑MS workflows

ecTOF Metabolomics with GC‑HRMS and Dual EI+CI Insight

<p><strong>The aromatic profile of Trigonella caerulea (blue fenugreek) develops predominantly after harvest, yet its detailed chemical characterization has long been limited by analytical challenges associated with highly volatile and low-abundance compounds. In this study, conventional GC–MS headspace analysis was complemented by high-resolution GC–MS employing cyanopropyl-functionalized stationary phases to improve compound separation and identification.</strong></p><p>While standard GC–MS enabled the detection of major volatile aldehydes and monoterpenoids, high-resolution GC–MS proved essential for resolving co-eluting compounds and identifying very volatile aroma constituents with greater confidence. In parallel, non-volatile α-keto acids were quantified by LC–MS to provide a comprehensive view of aroma precursors.</p><p>Comparative analysis demonstrates that high-resolution GC–MS significantly enhances analytical depth and accuracy, allowing a more complete understanding of aroma formation dynamics during drying and storage. These results underline the critical role of advanced chromatographic techniques in elucidating complex herbal aroma systems.</p><h4><strong>Learning points:</strong></h4><ul><li><strong>Analytical resolution matters:</strong> High-resolution GC–MS provides superior chromatographic separation and compound identification compared to conventional GC–MS, enabling reliable detection of highly volatile and low-abundance aroma compounds that would otherwise remain unresolved.</li><li><strong>Method choice shapes interpretation: </strong>The apparent aromatic profile of Trigonella caerulea depends strongly on the analytical technology used, with advanced GC–MS approaches revealing a substantially higher chemical complexity than standard methods.</li><li><strong>Integrated analytics are essential: </strong>Combining high-resolution GC–MS for volatile compounds with LC–MS for non-volatile aroma precursors allows a more complete understanding of aroma formation dynamics during drying and storage.</li></ul><h4><strong>Who should attend?</strong></h4><ul><li>Analytical chemists and method developers&nbsp;</li><li>Food, flavor, and fragrance scientists&nbsp;</li><li>Natural products and phytochemistry researchers&nbsp;</li></ul><p><i>If you cannot attend this webinar please register to receive a link to the On Demand version the following day.</i></p><blockquote><p><strong>Presenter: </strong>Dr. Thomas Stegemann (Lab Manager and group leader, Christian-Alberts-University Kiel)</p><p>Thomas has a Dr. in pharmacy, deep GC and GC/MS knowledge and combines both assets working for the University in cooperation with Pharma companies</p><p><strong>Presenter:&nbsp;</strong>Petra Gerhards (Regional Marketing Manager, EMEA, AAT, Thermo Fisher Scientific)</p></blockquote>

Thermo Fisher Scientific: Aromatic profiling using GCMS and HRGCMS

Aromatic profiling using GCMS and HRGCMS

Learn how HRMS GC–MS and LC–MS improve characterization of blue fenugreek aroma compounds and reveal complex flavor formation during drying and storage.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Looking to further elevate your Thermo Scientific Chromeleon CDS skills? Want to learn more about the administration tools for your Chromeleon software setup? Already attended </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5981"><strong>Administration Console I webinar (tips, shortcuts, and user/access management)</strong></a><strong>? Part II is the next step – focused on system-level management, structured resources, and automation across your Chromeleon software environment.</strong></p><p>In this practical session, we’ll dive into more Administration Console capabilities that help you manage and standardize your setup at scale – from Data Vault configuration and Chromeleon Resources to eWorkflows and automated rollout of updates. You’ll leave with proven approaches for keeping multi-station environments consistent, controlled, and easier to support.</p><h4><strong>What’s different in Part II (vs. </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5981"><strong>Part I</strong></a><strong>):</strong></h4><ul><li><strong>Beyond users &amp; permissions:</strong> Shift from access management to managing Chromeleon Resources and system components.</li><li><strong>System structure &amp; governance: </strong>Learn how Data Vaults support secure, controlled data handling and standardized operations.</li><li><strong>Remote administration: </strong>Explore methods for remotely updating Chromeleon Stations and reducing manual touchpoints.</li><li><strong>Real-world best practices: </strong>Practical guidance to help maintain stability, security, and compliance as environments grow.</li></ul><h4><strong>Who should attend:</strong></h4><p>This session is designed for anyone wanting to learn more about administration of Chromeleon CDS, administrators, lab IT/support teams, and power users who want to take on administration responsibilities – especially those supporting multiple stations or managing standardized deployments.</p><p>Get practical, immediately usable techniques to keep your Chromeleon software setup <strong>scalable, consistent, and supportable</strong> – with less manual effort and fewer surprises.</p><p><i>Cannot attend the broadcast on March 26? Please register anyway to automatically receive the link to the recording.</i></p><blockquote><p><strong>Presenter: </strong>Pascal Bächli (Sr. Engineer, Field Applications • Analytical Instrruments Group, Thermo Fisher Scientific)</p><p>Pascal Bächli is a Sr. Field Application Engineer at Thermo Fisher Scientific with over 12 years of laboratory experience in both GMP and non-GMP environments. He specializes in the administration, implementation, and support of laboratory data systems, including Thermo Scientific Chromeleon CDS and Waters Empower. He supports networked Chromeleon software installations, delivering consulting and user training, and troubleshooting for complex technical and workflow issues.</p><p>Pascal also holds a degree in Business IT, complementing his strong lab background and scientific expertise with deep knowledge of digital systems and business processes. This combination allows him to bridge the gap between the practical challenges scientists face and available technology to deliver efficient and compliant laboratory data solutions.</p></blockquote></div></div></div>

Thermo Fisher Scientific: Chromeleon Admin Console II: System management and automation

Chromeleon Admin Console II: System management and automation

In this practical session, we’ll dive into more Administration Console capabilities that help you manage and standardize your setup at scale

Chromeleon Admin Console II: System management & automation

<p><strong>Detecting gas leaks quickly and accurately is essential for safe operations, regulatory compliance, and emissions reduction. Optical gas imaging (OGI) cameras are an outstanding technology for assessing potential leaks in complex or potentially hazardous settings.</strong></p><p>In this webinar, Thermo Fisher Scientific and Opgal experts will discuss the benefits of handheld OGI cameras for real-time visualization of gas leaks in oil &amp; gas and industrial environments. The session will highlight how solutions such as the Opgal EyeCGas Multi, CO, CO₂, and long-range cameras enable faster inspections, improved leak detection efficiency, and safer, non-intrusive monitoring to support LDAR programs and methane reduction initiatives.</p><h4><strong>The webinar will cover these key topics and more:</strong></h4><ul><li>How handheld optical gas imaging works and where it adds the most value</li><li>Key advantages of handheld OGI compared to traditional leak detection methods</li><li>Typical applications for methane, CO, and CO₂ detection</li><li>How OGI supports LDAR, safety, and emissions compliance programs</li><li>Practical considerations when selecting a handheld OGI camera</li></ul><blockquote><p><strong>Presenter: </strong>Bob Gallagher</p><p>Bob Gallagher joined Thermo Fisher Scientific as the Product Line Manager for the Industrial Hygiene Strategy at the end of 2017. &nbsp;Bob has over 35 years of experience in Engineering and Product Management roles in the area of LDAR, site remediation, dust monitoring and other various industrial applications. &nbsp;Over Bob’s career, he’s worked for various technical companies such as Texas Instruments and Pentair and has successfully launched several innovative global products. &nbsp;Bob has a BS in Materials Science &amp; Engineering from Penn State University and an MBA from Boston University.</p><p><strong>Presenter: </strong>Michael Tsudikov</p><p>Michael Tsudikov is the Global Sales Director at Opgal, a Gevasol company, where he leads global sales and business development for advanced thermal and optical gas imaging solutions. He brings over seven years of experience driving growth through strategic partnerships, market expansion, and customer-focused sales initiatives. With a background spanning highly regulated industries and international markets, Michael is skilled at aligning innovative technologies with real-world operational and compliance needs. He is passionate about collaboration, innovation, and delivering high-quality solutions that create meaningful impact across industrial and environmental applications.</p></blockquote>

Thermo Fisher Scientific: Seeing Gas Leaks in Real Time: How Handheld Optical Gas Imaging Improves LDAR

Seeing Gas Leaks in Real Time: How Handheld Optical Gas Imaging Improves LDAR

Thermo Fisher Scientific and Opgal experts will discuss the benefits of handheld OGI cameras for real-time visualization of gas leaks in oil & gas.

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