<div><p><strong>In 2025, two outstanding young female researchers, Drs. Filipa Oliveira and Stella Gonsales, both originally from outside the Czech Republic, received prestigious JUNIOR STAR grants from the Czech Science Foundation (GAČR). JUNIOR STAR enables early career researchers to establish their own independent research groups and significantly advance their scientific careers. Their projects reflect cutting-edge research in materials science and sustainable chemistry, highlighting the international reach and welcoming research environment at UCT Prague.</strong></p><p><strong>Dr. Stella Gonsales is also a&nbsp;laureate of the Dagmar Procházková Fund, which supports promising scientific projects in sustainable and advanced organic chemistry. Another recipient of this grant is Dr.&nbsp;Anna Valunchene, whose research complements UCT Prague’s&nbsp;sustainability goals. This dual funding underlines Stella’s&nbsp;remarkable achievements and the wide scope of innovative research at UCT Prague.</strong></p><p><strong>Both Filipa and Stella are actively looking for motivated students and researchers to join their teams, offering a&nbsp;unique opportunity to work at the forefront of science in a&nbsp;vibrant and supportive environment.</strong></p></div><div><h3><strong>Interview with Filipa Oliveira: Pioneering tubular MXenes for supercapacitor energy storage</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Filipa Oliveira" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_2_da6e538579.jpeg 235w," sizes="100vw" width="1500" height="999"></p><h5><strong>Congratulations on receiving the JUNIOR STAR grant. What does this achievement mean for you and how will it influence your next steps?</strong></h5><p>Thank you. It’s&nbsp;a huge achievement to receive this prestigious grant. It gives me the opportunity to establish my own research group here at UCT Prague. The JUNIOR STAR program supports talented young scientists in building their teams and accessing resources. This means I&nbsp;now have the support to focus fully on my research about tubular MXenes and their application in supercapacitors.</p><h5><strong>Why are tubular MXenes so innovative for energy storage?</strong></h5><p>MXenes are a family of 2D materials with a layered, accordion-like structure and known for their excellent electrical conductivity. Our innovation is to produce them with a tubular morphology, combining the high conductivity and redox activity in superior ion-transport tubular architectures. Their open, hollow structure increases surface area, ion diffusion and charge transport. This could translate to faster, more efficient supercapacitors, critical for sustainable energy technologies.</p><h5><strong>How did your scientific interest lead you to this field?</strong></h5><p>Global challenges around green energy made me focus on materials that can improve energy storage. MXenes stood out because changes in the composition and structure can significantly change their properties. I&nbsp;was curious about how tuning different metallic elements in a&nbsp;tubular morphology can impact how MXenes perform and how to optimize them for practical applications.</p><h5><strong>What challenges have you encountered?</strong></h5><p>One major challenge is their stability: MXenes oxidize quickly which deteriorates performance. Another challenge is understanding how synthesis conditions of both MXenes and their precursors (MAX phases) must be adapted to create and preserve tubular morphologies since their synthesis mechanisms differ from conventional MXenes. Additionally, effectively communicating the project in the funding proposal to show to reviewers why I&nbsp;should obtain this funding was also a&nbsp;challenge, but thanks to mentorship and collaboration, I&nbsp;overcame these hurdles.</p><h5><strong>What motivates you most about working at UCT Prague?</strong></h5><p>The support from colleagues and the excellent technical infrastructure make it a&nbsp;great place to work. Also, being a&nbsp;former member of Professor Sofer’s&nbsp;leading group in 2D&nbsp;materials is inspiring and led me to this achievement.</p><h5><strong>How do&nbsp;you see your professional development going in the future?</strong></h5><p>The grant will help me build my own team, acquire equipment, and develop collaborations. My long-term vision is to contribute significantly to sustainable energy solutions and scientific progress both locally and internationally.</p><h5><strong>You are looking for new team members. What kind of students or scientists do&nbsp;you need?</strong></h5><p>We are currently welcoming enthusiastic master's&nbsp;students, PhD candidates, and postdocs who are passionate about materials science and eager to explore new frontiers in MXenes and energy storage. If you love curiosity-driven research and want to be part of a&nbsp;growing international team, we encourage you to join us.</p><h3><strong>Interview with Stella Gonsales: Catalysis as a&nbsp;key to polymer recycling</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg" alt="VŠCHT Prague: JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science: Stella Gonsales" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_V_prestizni_soutezi_GACR_uspely_dve_zahranicni_vedkyne_z_VSCHT_3_3d0ded6274.jpeg 233w," sizes="100vw" width="1499" height="1000"></p><h5><strong>Congratulations on your JUNIOR STAR grant and also being awarded the Dagmar Procházková Fund. What does receiving both mean to you and how will they shape your work?</strong></h5><p>Thank you. Both grants are hugely important. The JUNIOR STAR grant allows me to expand my group and resources for research into catalytic recycling of polymers, while the Dagmar Procházková Fund supports complementary projects such as synthesizing new biodegradable polymers. Together, they help tackle the plastic waste problem from multiple scientific angles.</p><h5><strong>Could you explain your research in layperson’s&nbsp;terms?</strong></h5><p>Most plastics have strong carbon-carbon bonds. We insert more cleavable double bonds into polymers that can be broken down by catalytic reactions called olefin metathesis, making recycling on a&nbsp;molecular level more feasible.</p><h5><strong>What makes the JUNIOR STAR grant particularly important for your research and career development?</strong></h5><p>The JUNIOR STAR grant is a&nbsp;prestigious and highly competitive funding opportunity aimed at excellent early-career scientists within eight years of completing a&nbsp;Ph.D. It provides up to 25&nbsp;million CZK over five years, allowing recipients to establish independent research groups and pursue innovative scientific topics. This grant is crucial for building a&nbsp;solid foundation, acquiring necessary equipment, and recruiting talented team members, enabling the pursuit of ambitious projects that can significantly contribute to both the Czech scientific community and global challenges.</p><h5><strong>What collaborations support your research?</strong></h5><p>The collaboration between UCT Prague and the Institute of Organic Chemistry and Biochemistry (IOCB) of the Czech Academy of Sciences primarily provides financial and technical support. The cooperation helps with funding, access to advanced equipment, and operational resources needed for running research projects.</p><h5><strong>What advice would you give young scientists aiming to succeed?</strong></h5><p>I&nbsp;encourage thinking outside the box and combining diverse knowledge you acquire during your career to address complex problems that are exciting and relevant to you.</p><h5><strong>Your laboratory bears the name of Otto Wichterle and was ceremonially opened in the presence of Professor Kamil Wichterle. How does Wichterle’s&nbsp;legacy inspire you personally?</strong></h5><p>Wichterle’<s>’</s>s&nbsp;innovative spirit motivates me to push creative boundaries and pursue novel solutions in sustainable chemistry.</p><h5><strong>What are your visions for the future?</strong></h5><p>I&nbsp;aim to build a&nbsp;research group that advances sustainable polymer recycling, contributing to environmental responsibility and the circular economy.</p><h5><strong>You are recruiting people for your team. What skills do&nbsp;they need to have?</strong></h5><p>We are looking for talented Ph.D. students and postdocs experienced in air-sensitive catalysis techniques. If you want to contribute to transformative research in sustainable chemistry and join a&nbsp;dynamic team, this is a&nbsp;great opportunity.</p></div>

JUNIOR STAR Grants at UCT Prague: Two talented women from abroad driving innovation in science

News from LabRulezGCMS Library - Week 06, 2026

<p><strong>Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal </strong><i><strong>Nature Chemical Biology</strong></i><strong>.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.</p><p>In their study,<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en"><strong>Dr. Hana Cahová</strong></a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.</p><p>The work was significantly shaped by <strong>Valentina Serianni from Hana Cahová’s team</strong>, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by <strong>Jana Škerlová</strong>, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.</p><p>Hana Cahová adds: <i>“We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”</i></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by<strong> Dr. Tomáš Kouba</strong>, one of the authors of the study. <i>“Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,”</i> he explains.</p><p>Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en"><strong>cryo-EM center</strong></a><strong> </strong>that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"></p><blockquote><h4><strong>The Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5"><strong>Molecular insight into 5′ RNA capping with Np<sub>n</sub>Ns by bacterial RNA polymerase</strong></a></h4><p>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;</p><p>Nat. Chem. Biol. 21 (2026)</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from <i>Thermus</i> <i>thermophilus</i>. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</p></div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

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The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

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Phenomenex Strata Screen-A Solid Phase Extraction (SPE)

Incorporates the hydrophobic selectivity of a C8 phase and strong anion-exchange for the extraction of acidic drugs from biological matrices.

<p><strong>Mixed-Mode Solid Phase Extraction (SPE) Sorbent</strong></p><p>Incorporates the hydrophobic selectivity of a C8 phase and strong anion-exchange for the extraction of acidic drugs from biological matrices.</p><figure class="image"><img style="aspect-ratio:500/150;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_Screen_A_f370bdfd48.webp" alt="Phenomenex: Strata Screen-A" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_Screen_A_f370bdfd48.webp 245w," sizes="100vw" width="500" height="150"></figure><ul><li><strong>Surface Area (m²/g)</strong>: -</li><li><strong>Separation Mode:</strong> Multi-Mode</li><li><strong>Particle Size:</strong> 55</li><li><strong>Recommended Use: </strong>Extracts acidic drugs from biological matrices</li></ul><h4><strong>Starting Method</strong></h4><h5><strong>Strata Screen-A Strong Anion-Exchange</strong></h5><ul><li><strong>Condition:</strong> 1 mL Methanol</li><li><strong>Equilibrate:</strong> 1 mL Water</li><li><strong>Load:</strong> Pretreated sample</li><li><strong>Wash 1</strong>: 1 mL 25mM Ammonium Acetate Buffer, pH 6-7</li><li><strong>Wash 2</strong>: 1 mL Methanol, dry under vacuum for 2-5 min</li><li><strong>Elute: </strong>1 mL 5% Formic Acid in Methanol</li></ul><p>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.</p>

Phenomenex Strata Screen-C Solid Phase Extraction (SPE)

Incorporates the hydrophobic selectivity of a C8 phase and strong cation exchange for the extraction of basic drugs from biological matrices.

<p><strong>Strong Cation-Exchange Solid Phase Extraction (SPE)</strong></p><p>Incorporates the hydrophobic selectivity of a C8 phase and strong cation exchange for the extraction of basic drugs from biological matrices.</p><figure class="image"><img style="aspect-ratio:500/150;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_Screen_C_b60bb2b7e4.webp" alt="Phenomenex: Strata Screen-C" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_Screen_C_b60bb2b7e4.webp 245w," sizes="100vw" width="500" height="150"></figure><ul><li><strong>Surface Area (m²/g)</strong>: 500, m²/g, 500 m²/g</li><li><strong>Separation Mode:</strong> Multi-Mode</li><li><strong>Pore Size</strong>: 70</li><li><strong>Particle Size:</strong> 55</li><li><strong>Recommended Use: </strong>Extraction of basic drugs from biological matrixes</li></ul><h4><strong>Starting Method</strong></h4><h5><strong>Strata Screen-C Strong Cation-Exchange</strong></h5><ul><li><strong>Condition:</strong> 1 mL Methanol</li><li><strong>Equilibrate:</strong> 1 mL Acidified Water</li><li><strong>Load:</strong> Pretreated sample (acidified)</li><li><strong>Wash 1</strong>: 1 mL 0.1N HCl in Water</li><li><strong>Wash 2</strong>: 1 mL 0.1N HCl in Methanol, dry under vacuum for 2-5 min</li><li><strong>Elute: </strong>1 mL 5 % NH<sub>4</sub>OH in Methanol</li></ul><p>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.</p>

Phenomenex Strata Screen-C GF Solid Phase Extraction (SPE)

Optimized for gravity flow, this sorbent offers hydrophobic selectivity from a C8 phase and strong cation-exchange properties for the extraction of basic drugs.

<p><strong>Strong Cation-Exchange Solid Phase Extraction (SPE)</strong></p><p>Optimized for gravity flow, this sorbent offers hydrophobic selectivity from a C8 phase and strong cation-exchange properties for the extraction of basic drugs from biological matrixes. Offers the selectivity of Screen-C in a gravity flow particle size for viscous samples.</p><h4><strong>Starting Method</strong></h4><h5><strong>Strata Screen-C GF Strong Cation-Exchange</strong></h5><ul><li><strong>Condition:</strong> 1 mL Methanol</li><li><strong>Equilibrate:</strong> 1 mL Acidified Water</li><li><strong>Load:</strong> Pretreated sample (acidified)</li><li><strong>Wash 1</strong>: 1 mL 0.1N HCl in Water</li><li><strong>Wash 2</strong>: 1 mL 0.1N HCl in Methanol, dry under vacuum for 2-5 min</li><li><strong>Elute: </strong>1 mL 5 % NH<sub>4</sub>OH in Methanol</li></ul><p>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.</p>

Waters Oasis SPE products

Waters Oasis SPE products come in a broad range of sorbents and an array of formats. With Oasis products, you can meet a wide variety of sample preparation needs.

<h4><strong>Faster, cleaner, and simpler SPE starts here</strong></h4><p>Analytical chemists worldwide use SPE to remove interfering matrix compounds and enrich the analyte of interest to ensure the generation of high-quality data.</p><p>Waters Oasis SPE products come in a broad range of sorbents and an array of formats. With Oasis products, you can meet a wide variety of sample preparation needs, from simple and fast sample matrix clean-up to more complex sample preparation challenges.</p><p>Oasis SPE products deliver unmatched purity, consistency, and quality for sample analysis. Along with seven different SPE chemistries, Oasis SPE products use universal to customized protocols to handle your unique sample.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_SPE_kolonky_2_967d461f2e/Waters-Oasis-SPE-kolonky-2.jpg" alt="Waters: Oasis SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_SPE_kolonky_2_967d461f2e/thumbnail_Waters-Oasis-SPE-kolonky-2.jpg 245w," sizes="100vw" width="950" height="416"></p><h4><strong>Overview</strong></h4><ul><li>Fully water-wettable sorbents increase reproducibility and enable elimination of condition and equilibration steps</li><li>Co-polymer pH stable from 0–14 with no secondary silanol activity</li><li>Optimal sensitivity for small molecule and therapeutic peptide analysis</li><li>Reduced matrix effects for LC-MS/MS analyses</li><li>Unsurpassed selectivity</li><li>Consistent retention and capacity, yielding the highest SPE recovery</li><li>Reduces variability in analytical results and increases robustness of analysis</li><li><strong>Recommended Use: </strong>For the fastest, cleanest, and simplest approach to SPE-LC-MS/MS method development.</li></ul><h4><strong>Solid-Phase Extraction Chemistries</strong></h4><h5><strong>Oasis PRiME HLB</strong></h5><ul><li>Multi-purpose hydrophilic-lipophilic balanced reversed phase sorbent for acids, bases, and neutrals</li><li>Cleaner samples in fewer steps with simple three-and two-step protocols, no SPE expertise required</li><li>Removes 95% of common matrix interferences, such as proteins, salts, phospholipids, and pigments</li><li>No sorbent conditioning or equilibration steps required</li><li>Faster, more predictable analysis time</li><li>Ideal for reversed-phase SPE clean-up of samples in routine analysis.</li></ul><h5><strong>Oasis PRiME MCX</strong></h5><ul><li>Mixed-mode Cation eXchange sorbent to selectively retain, concentrate and elute basic compounds</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Cleaner samples in fewer steps with simple and fast 3 and 4-step protocols</li><li>Removes 99% of phospholipids from complex sample matrices</li><li>No sorbent conditioning or equilibration steps required</li><li>Faster, more predictable analysis time</li><li>Highly reproducible target analyte recoveries</li><li>Ideal for basic compound specificity while removing matrix interferences in a simple clean-up.</li></ul><h5><strong>Oasis HLB</strong></h5><ul><li>Multi-purpose hydrophilic-lipophilic balanced reversed phase sorbent</li><li>Provides high capacity for acids, bases, and neutrals</li><li>Ideal for ultra-high capacity of very polar compounds.</li></ul><h5><strong>Oasis MCX</strong></h5><ul><li>Mixed-mode Cation eXchange sorbent for bases</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for selective retention of basic analytes.</li></ul><h5><strong>Oasis MAX</strong></h5><ul><li>Mixed-mode Anion eXchange for acids</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for selective retention of acidic analytes.</li></ul><h5><strong>Oasis WAX</strong></h5><ul><li>Weak Anion eXchange mixed-mode sorbent for strong acidic compounds</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for improved retention of strong acidic compounds.</li></ul><h5><strong>Oasis WCX</strong></h5><ul><li>Weak Cation eXchange mixed-mode sorbent for strong basic compounds</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for improved retention of all basic analytes, especially strong bases.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Sorbenty_Oasis_SPE_na_bazi_kopolymeru_Oasis_HLB_c8ffee39b3/Waters-Sorbenty-Oasis-SPE-na-bazi-kopolymeru-Oasis-HLB.png" alt="Waters: Oasis Sorbents" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Sorbenty_Oasis_SPE_na_bazi_kopolymeru_Oasis_HLB_c8ffee39b3/thumbnail_Waters-Sorbenty-Oasis-SPE-na-bazi-kopolymeru-Oasis-HLB.png 211w," sizes="100vw" width="1511" height="1119"></p><h4><strong>Oasis Formats</strong></h4><h5><strong>Oasis μElution Plate</strong></h5><ul><li>Patented μElution plate design</li><li>No evaporation and reconstitution necessary due to elution volumes as low as 25 µL</li><li>Ideal for SPE clean-up and analyte enrichment of small sample volumes</li><li>Compatible with most liquid-handling robotic systems for automated, reliable high-throughput SPE (HT-SPE)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_m_Elution_Plate_aa7e36a4c1.jpg" alt="Waters: Oasis µElution Plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_m_Elution_Plate_aa7e36a4c1.jpg 245w," sizes="100vw" width="502" height="260"></p><h5><strong>Oasis 96-Well Sample Extraction Plates</strong></h5><ul><li>Innovative, award-winning two-stage well design</li><li>High throughput and high recovery</li><li>Compatible with most liquid-handling robotic systems for automated, reliable high-throughput SPE (HT-SPE)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_96_Well_Sample_Extraction_Plates_9528d532b9.jpg" alt="Waters: Oasis 96-Well Sample Extraction Plates" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_96_Well_Sample_Extraction_Plates_9528d532b9.jpg 245w," sizes="100vw" width="525" height="329"></p><h5><strong>Oasis Syringe-Barrel Cartridges</strong></h5><ul><li>Ultra-clean syringe barrel and frits</li><li>Available in cartridges ranging from 1 cc to 60 cc</li><li>Flangeless syringe-barrel cartridges available in 1 cc, 3 cc, and 6 cc sizes</li><li>Plus-style cartridges with Luer inlet hub and outlet tip, compatible with hand-held syringes</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_Syringe_Barrel_Cartridges_961aa06b8a.jpg" alt="Waters Corporation: Oasis Syringe-Barrel Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_Syringe_Barrel_Cartridges_961aa06b8a.jpg 245w," sizes="100vw" width="503" height="311"></p><h5><strong>Oasis Glass Cartridges</strong></h5><ul><li>Ultra-clean glass syringe with Teflon® frit</li><li>For trace level detection and analysis at part-per-trillion (PPT) levels</li><li>Available in 5 cc (200 mg) configuration</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_Glass_Cartridges_5e96319c7f.jpg" alt="Waters: Oasis Glass Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_Glass_Cartridges_5e96319c7f.jpg 188w," sizes="100vw" width="409" height="339"></p><h5><strong>Oasis On-Line Columns and Cartridges</strong></h5><ul><li>For rugged, reproducible, and ultra-fast on-line analysis</li><li>Wide choice of configurations, particle sizes, and sorbent chemistries</li><li>Available with all six patented Oasis sorbents—PRiME HLB, HLB, MCX, MAX, WCX, and WAX</li><li>High recovery and reproducible results for a wide range of compounds</li><li>Cartridge format for use with Spark Holland Prospekt-2™/Symbiosis™ systems also available</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_On_Line_Columns_and_Cartridges_ba02114c92.jpg" alt="Waters: Oasis On-Line Columns and Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_On_Line_Columns_and_Cartridges_ba02114c92.jpg 168w," sizes="100vw" width="415" height="386"></p><h4><strong>Sample preparation, improved</strong></h4><p>Improving various aspects of chromatography and mass spectrometry analyses often starts with sample preparation. &nbsp;Solid-phase extraction (SPE) or "liquid-solid phase extraction" is a sample preparation technology that uses particles of chromatographic packing material, usually in a cartridge or 96-well plate format, to chemically separate different analytes and aid in removing matrix components in a sample. &nbsp;</p><p>SPE is a proven sample preparation tool for simple sample matrix removal, including common interferences like proteins, salts, fats and phospholipids. This matrix removal provides many benefits, including matrix effect reduction and an increase in sample concentration and analyte specificity. Matrix removal also significantly simplifies chromatographic complexity by reducing the number of interfering peaks in the LC and reducing signal suppression by providing clean samples for the MS.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_SPE_LC_MS_MS_chromatogram_of_1_pgm_L_of_alprazolam_from_rat_plasma_8b7275b25b.jpg" alt="Waters: Oasis SPE - LC-MS-MS chromatogram of 1 pgmL of alprazolam from rat plasma" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_SPE_LC_MS_MS_chromatogram_of_1_pgm_L_of_alprazolam_from_rat_plasma_8b7275b25b.jpg 169w," sizes="100vw" width="1024" height="946"></p><h4><strong>Get the cleanest extracts with the Oasis 2x4 method</strong></h4><p>Having a variety of tools for method development allows separation scientists to optimize protocols for maximum sensitivity and selectivity. The Oasis 2x4 method for SPE sorbent and protocol selection for acids, bases, and neutrals produces the cleanest extracts – all by eliminating matrix interferences and sample variability. Get the flexibility to extract acids, bases, and neutrals with high SPE recoveries while removing matrix components that may interfere with analysis with our two protocols and four sorbents. By following the simple steps outlined in the Oasis 2x4 method, you can achieve high recoveries and the cleanest extracts.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_2x4_protokol_pro_vyvoj_metody_c417be9006/Waters-Oasis-2x4-protokol-pro-vyvoj-metody.jpg" alt="Waters: Oasis SPE - Oasis 2 x 4 method development protocol." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_2x4_protokol_pro_vyvoj_metody_c417be9006/thumbnail_Waters-Oasis-2x4-protokol-pro-vyvoj-metody.jpg 242w," sizes="100vw" width="1519" height="979"></p><h4><strong>Isolate your compounds with the Oasis HLB method</strong></h4><p>Solid-phase extraction (SPE) method development starts with knowledge of the sample matrix—the nature and relative concentration of the analytes of interest and potentially interfering endogenous compounds. The right sorbent and solvents are selected for load, wash, and elute steps, resulting in the creation of rugged, reliable, and selective protocols. The Oasis HLB method is a generic protocol used to isolate a broad range of acidic, basic, and neutral compounds from a wide spectrum of matrices.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_SPE_Generic_Method_Protocol_with_Oasis_HLB_SPE_83d684704d.jpg" alt="Waters: Oasis SPE - Generic Method Protocol with Oasis HLB SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_SPE_Generic_Method_Protocol_with_Oasis_HLB_SPE_83d684704d.jpg 139w," sizes="100vw" width="1024" height="1153"></p><h4><strong>Multiple contaminant analysis with the 2-step pass-through protocol</strong></h4><p>If you are doing multiple residue screening of veterinary drugs, pesticides and mycotoxins in various food matrices, you may be facing a high concentration of organic solvents. With our simple 2-step protocol (pass-through SPE), you can remove matrix interferences quickly if your samples contain a high concentration of organic solvent(s) and concentration and/or salt removal is not required. It can be used as the clean-up method following QuEChERS extraction, saving you significant time compared to the traditional dispersive SPE method - all while achieving superior clean-up.</p><p><strong>To do pass-through SPE, Waters offers 2 convenient and flexible formats:</strong></p><ul><li><strong>Traditional-style cartridges</strong> compatible with vacuum and positive pressure manifolds&nbsp;</li><li><strong>"Plus" style cartridges</strong> that can be used without manifolds when connected to a displacement syringe</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_SPE_kolonky_3_b4da6242de/Waters-Oasis-SPE-kolonky-3.jpg" alt="Waters: Oasis Plus Light and Plus Short Cartridges for 2-step pass through protocol." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_SPE_kolonky_3_b4da6242de/thumbnail_Waters-Oasis-SPE-kolonky-3.jpg 152w," sizes="100vw" width="1308" height="1342"></p>

Phenomenex Strata X-AW Solid Phase Extraction (SPE)

Strata-X-AW mixed-mode SPE sorbent enables strong retention of acids (pKa ≤5) with 100% organic wash, ensuring maximum removal of interferences for clean, reliable extractions.

<p><strong>Weak Anion Mixed-Mode Solid Phase Extraction (SPE)</strong></p><p>A weak anion-exchange functionalized polymeric sorbent that allows for complete retention of acidic compounds with a <strong>pKa less than 5</strong>, making <strong>100% organic wash conditions possible</strong>. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_AW_SPE_cd5908db4d.jpg" alt="Phenomenex: Strata-X-AW SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_AW_SPE_cd5908db4d.jpg 245w," sizes="100vw" width="500" height="268"></p><ul><li><strong>Recommended Use: </strong>Extraction of acidic compounds, particularly strong acids</li></ul><h4><strong>Starting Method</strong></h4><h5><strong>For Acids with pKa ≤ 5</strong></h5><ul><li><strong>Column: </strong>1 mL Methanol</li><li><strong>Equilibrate: </strong>1 mL Water, pH 6-7</li><li><strong>Load: </strong>Diluted Sample pH 6-7</li><li><strong>Wash 1: </strong>1 mL 25 mM Ammonium Acetate Buffered, pH 6-7</li><li><strong>Wash 2: </strong>1 mL Methanol</li><li><strong>Elute Any Acid: </strong>2x 500 µL 5 % NH<sub>4</sub>OH in Methanol</li><li><strong>Elute Weak Acids: </strong>2x 500 µL 5 % Formic Acid in Methanol</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080"></p><p><i>Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.</i></p><h4><strong>Available Formats</strong></h4><ul><li><strong>96-Well-Plate</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"></p><ul><li><strong>1, 3, and 6mL Tubes</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"></p><ul><li><strong>Giga Tubes (12-150mL Tubes)</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080"></p>

Phenomenex Strata X-A Solid Phase Extraction (SPE)

Strata-X-A strong anion-exchange SPE retains weak acids (pKa >2) with 100% organic wash, maximizing interference removal. Available in tubes, plates, and giga formats.

<p><strong>Strong Anion Mixed-Mode Solid Phase Extraction (SPE)</strong></p><p>A strong anion-exchange functionalized polymeric sorbent that allows for complete retention of weakly acidic compounds with a <strong>pKa greater than 2</strong>, making <strong>100% organic wash conditions possible</strong>. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_A_SPE_e107f0b6bf.jpg" alt="Phenomenex: Strata-X-A SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_A_SPE_e107f0b6bf.jpg 245w," sizes="100vw" width="500" height="268"></p><ul><li><strong>Recommended Use: </strong>Extraction of weakly acidic compounds</li></ul><h4><strong>Starting Method</strong></h4><h5><strong>For Acids with pKa &gt; 2</strong></h5><ul><li><strong>Column: </strong>1 mL Methanol</li><li><strong>Equilibrate: </strong>1 mL Water, pH 6-7</li><li><strong>Load: </strong>Diluted Sample pH 6-7</li><li><strong>Wash 1: </strong>1 mL 25 mM Ammonium Acetate Buffered, pH 6-7</li><li><strong>Wash 2: </strong>1 mL Methanol (collect this fraction to analyze Neutral/Bases)</li><li><strong>Elute Acid: </strong>2x 500 µL 5 % Formic Acid in Methanol</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080"></p><p><i>Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.</i></p><h4><strong>Available Formats</strong></h4><ul><li><strong>96-Well-Plate</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"></p><ul><li><strong>1, 3, and 6mL Tubes</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"></p><ul><li><strong>Giga Tubes (12-150mL Tubes)</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080"></p>

Phenomenex Strata X-C Solid Phase Extraction (SPE)

Strata-X-C strong cation-exchange SPE retains basic compounds (pKa ≤10.5) with 100% organic wash for maximum cleanup. Offered in tubes, plates, and giga formats.

<p><strong>Strong Cation Mixed-Mode Solid Phase Extraction (SPE)</strong></p><p>A strong cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a <strong>pKa less than 10.5,</strong> making <strong>100% organic wash conditions possible</strong>. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_C_SPE_4927d7340d.jpg" alt="Phenomenex: Strata-X-C SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_C_SPE_4927d7340d.jpg 245w," sizes="100vw" width="500" height="268"></p><ul><li><strong>Recommended Use: </strong>Extraction of weakly basic compounds</li></ul><h4><strong>Starting Method</strong></h4><h5><strong>For Bases with pKa ≤ 10.5</strong></h5><ul><li><strong>Column: </strong>1 mL Methanol</li><li><strong>Equilibrate: </strong>1 mL Acidified Water</li><li><strong>Load: </strong>Diluted Acidified Sample</li><li><strong>Wash 1: </strong>1 mL 0.1 N HCI in Water (collect this fraction to analyze Polar Neutrals)</li><li><strong>Wash 2: </strong>1 mL 0.1 N HCI in Methanol (collect this fraction to analyze Neutrals/Acids)</li><li><strong>Elute Bases: </strong>2x 500 µL 5 % NH4OH in Methanol</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080"></p><p><i>Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.</i></p><h4><strong>Available Formats</strong></h4><ul><li><strong>96-Well-Plate</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"></p><ul><li><strong>1, 3, and 6mL Tubes</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"></p><ul><li><strong>Giga Tubes (12-150mL Tubes)</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080"></p>

Phenomenex Strata X-CW Solid Phase Extraction (SPE)

Strata-X-CW weak cation-exchange SPE retains strong bases (pKa >8) with 100% organic wash for maximum cleanup. Available in tubes, 96-well plates, and giga formats.

<p><strong>Weak Cation Mixed-Mode Solid Phase Extraction (SPE)</strong></p><p>A weak cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a <strong>pKa greater than 8</strong>, making <strong>100% organic wash conditions possible</strong>. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_CW_SPE_b16a0e0baf.jpg" alt="Phenomenex: Strata-X-CW SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_CW_SPE_b16a0e0baf.jpg 245w," sizes="100vw" width="500" height="268"></p><ul><li><strong>Recommended Use: </strong>Extraction of basic compounds, particularly strong bases</li></ul><h4><strong>Starting Method</strong></h4><h5><strong>For Bases with pKa &gt; 8</strong></h5><ul><li><strong>Column: </strong>1 mL Methanol</li><li><strong>Equilibrate: </strong>1 mL Water, pH 6-7</li><li><strong>Load: </strong>Diluted Sample, pH 6-7</li><li><strong>Wash 1: </strong>1 mL Water, pH 6-7</li><li><strong>Wash 2: </strong>1 mL Methanol (collect this fraction to analyze Neutrals/Acids)</li><li><strong>Elute Any Bases: </strong>2x 500 µL 5 % Formic Acid in Methanol</li><li><strong>Elute Weak Bases: </strong>2x 500 µL 5 % NH4OH in Methanol</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080"></p><p><i>Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.</i></p><h4><strong>Available Formats</strong></h4><ul><li><strong>96-Well-Plate</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"></p><ul><li><strong>1, 3, and 6mL Tubes</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"></p><ul><li><strong>Giga Tubes (12-150mL Tubes)</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080"></p>

Phenomenex Strata X Solid Phase Extraction (SPE)

Strata-X reversed phase SPE retains neutrals, acids, and bases via π-π, hydrogen bonding, and hydrophobic interactions. Robust cleanup under high organic wash.

<p><strong>Reversed Phase Solid Phase Extraction (SPE)</strong></p><p>A reversed phase functionalized polymeric sorbent that gives strong retention of neutral, acidic, or basic compounds under aggressive, high organic wash conditions. This sorbent relies on <strong>3 mechanisms of retention</strong>: <strong>pi-pi bonding</strong>, <strong>hydrogen bonding</strong> (dipole-dipole interactions), and <strong>hydrophobic interaction</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_d8f4451e42.jpg" alt="Phenomenex: Strata-X" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_d8f4451e42.jpg 245w," sizes="100vw" width="433" height="247"></p><ul><li><strong>Recommended Use: </strong>Extraction of neutrals and aromatics</li></ul><h4><strong>Starting Method</strong></h4><h5><strong>For Neutral Compounds</strong></h5><ul><li><strong>Column: </strong>1 mL Methanol</li><li><strong>Equilibrate: </strong>1 mL Water</li><li><strong>Load: </strong>Diluted Sample</li><li><strong>Wash: </strong>1 mL 5-60 % Methanol</li><li><strong>Elute:</strong> 2x 500 µL 2 % Formic Acid in Methanol/Acetonitrile</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080"></p><p><i>Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.</i></p><h4><strong>Available Formats</strong></h4><ul><li><strong>96-Well-Plate</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"></p><ul><li><strong>1, 3, and 6mL Tubes</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"></p><ul><li><strong>Giga Tubes (12-150mL Tubes)</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080"></p>

Thermo Scientific QuEChERS Sample Preparation Kits

QuEChERS is a versatile sample preparation method for analyzing pesticides, veterinary drugs, and other residues in various sample types before GC-MS or LC-MS analysis.

<div><div><div><h3><strong>QuEChERS sample preparation—now easier, more convenient, and affordable</strong></h3><p><strong>QuEChERS</strong> (<strong><u>Q</u></strong><u>u</u>ick, <strong><u>E</u></strong>asy, <strong><u>C</u></strong>heap, <strong><u>E</u></strong>ffective, <strong><u>R</u></strong>ugged, and <strong><u>S</u></strong>afe)is a <strong>sample preparation method</strong> that was initially developed in 2003 for <strong>analyzing veterinary drugs in food</strong>. The method was also tested for <strong>pesticide residue analysis in plant material</strong> with great success. In 2003, the <strong>detailed method was published</strong> in the journal AOAC International. Since then, the QuEChERS method has evolved into a <strong>sample preparation technique used on a variety of analytes</strong> in an <strong>expanding number of sample types</strong> before analysis by <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/gas-chromatography-mass-spectrometry-gc-ms.html"><strong>GC-MS</strong></a><strong> or </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms.html"><strong>LC-MS</strong></a>.</p><p><strong>Thermo Scientific QuEChERS kits</strong> contain <strong>pre-packaged, ready-weighed salts, sorbents, and buffers</strong> to <strong>streamline your workflow</strong> and <strong>minimize the potential for error</strong>. The kits are grouped according to your <strong>analytes of interest</strong>, <strong>sample matrix</strong>, and <strong>preferred QuEChERS method</strong>:</p><ul><li><strong>Original 2003 Method</strong></li><li><strong>AOAC 2007.01 Method</strong></li><li><strong>EN 15662 Method</strong></li><li><strong>NY/T 1380-2007 Method</strong></li></ul><p><strong>Complete packages</strong>—including both <strong>extraction and clean-up kits</strong>—are available. <strong>Extraction-only and cleanup-only kits</strong> are also offered to suit your laboratory’s sample preparation needs. These <strong>pre-packaged kits save time and money</strong>, make QuEChERS procedures <strong>easier and more convenient</strong>, and ensure <strong>quality to yield reproducible results</strong> and <strong>excellent recoveries</strong> for a wide variety of analytes.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_2_1e99f0c81b.jpg" alt="Thermo Scientific QuEChERS Sample Preparation Kits" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_2_1e99f0c81b.jpg 217w," sizes="100vw" width="891" height="640"></p><h3><strong>Typical QuEChERS workflow</strong></h3><div><div><div><div><div><p>As shown in the workflow diagram below, the <strong>QuEChERS method has four basic steps</strong>:</p><ol><li><strong>Homogenize sample</strong> (fruit or vegetable)</li><li><strong>Extract analytes of interest</strong> from a sample using <strong>extraction salts</strong> (pesticide residues, veterinary drugs, and/or mycotoxins)</li><li><strong>Clean-up the resulting supernatant</strong> using <strong>dispersive solid phase extraction (dSPE)</strong></li><li><strong>Analyze by GC-MS or LC-MS</strong></li></ol><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_496491ceff.jpeg" alt="Thermo Scientific QuEChERS Sample Preparation Kits" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_496491ceff.jpeg 245w," sizes="100vw" width="1170" height="706"></p><div><h3><strong>QuEChERS products</strong></h3></div><div><h4><strong>QuEChERS extraction kits for original 2003 method</strong></h4></div><div><p>The <strong>original QuEChERS method</strong> (Anastassiades et al, 2003) was developed by <strong>Steven J. Lehotay and Michelangelo Anastassiades</strong> in 2003 for the <strong>determination of pesticide residues in fruits and vegetables</strong>. The original method was created to <strong>analyze non-base-sensitive compounds</strong> and uses a <strong>sodium chloride buffer</strong> to <strong>reduce polar interferences</strong> and <strong>enhance extraction</strong>. <strong>Buffering</strong> is an important part of the QuEChERS procedure because it <strong>allows for analysis of pesticides and other residues</strong> that display <strong>pH dependence in their recoveries</strong>, depending on the <strong>sample matrix</strong>. <strong>Three different extraction kits</strong> are available to support the <strong>original 2003 QuEChERS method</strong>.</p><h5><strong>Chemicals used in the original 2003 method</strong></h5></div><ul><li><strong>Acetonitrile:</strong> Used to perform single-phase extraction (SPE) on a sample</li><li><strong>Anhydrous MgSO₄:</strong> Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li><strong>NaCl:</strong> Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes residual water from sample; reduces polar interference and enhances extraction</li><li><strong>PSA:</strong> Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step</li></ul><div><h4><strong>QuEChERS extraction and clean-up kits for AOAC 2007.01 method</strong></h4><p>The <strong>AOAC 2007.01 QuEChERS Method</strong> was developed by <strong>Steven J. Lehotay and his co-workers</strong>, who <strong>modified the original 2003 method</strong> to accommodate the use of <strong>acetate buffering at higher concentration</strong> for <strong>greater buffering strength</strong>. <strong>Buffering</strong> is an important part of the QuEChERS procedure because it <strong>allows for analysis of pesticides and other residues</strong> that display <strong>pH dependence in their recoveries</strong>, depending on the <strong>sample matrix</strong>. These <strong>extraction and clean-up products</strong> here support the <strong>AOAC 2007.01 method</strong>.</p><div><h5><strong>Chemicals used in the AOAC 2007.01 method</strong></h5><ul><li><strong>Acetonitrile:</strong> Used to perform single-phase extraction on a sample</li><li><strong>Anhydrous MgSO₄:</strong> Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li><strong>NaOAc:</strong> Buffers the sample to stabilize pH</li><li><strong>PSA:</strong> Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step</li><li><strong>C18:</strong> Removes fats, sterols, and other non-polar interferences from sample</li><li><strong>GCB:</strong> Graphitized carbon black, removes pigment (<i>not recommended for use with planar pesticides</i>)</li></ul><div><h4><strong>QuEChERS extraction and clean-up kits for EN 15662 method</strong></h4><p>The <strong>extraction and clean-up products</strong> here support the <strong>EN 15662 method</strong>. <strong>Widely used in Europe</strong>, the EN 15662 method was developed by <strong>Michelangelo Anastassiades and co-workers</strong>, who <strong>modified the original 2003 method</strong> to accommodate the use of <strong>citrate buffering at a relatively low buffering capacity</strong>. This variation uses a <strong>sodium chloride buffer</strong> to <strong>minimize polar interferences</strong> in addition to <strong>sodium citrate dihydrate</strong> and <strong>disodium citrate sesquihydrate buffers</strong>. <strong>Buffering</strong> is an important part of the QuEChERS procedure because it <strong>allows for analysis of pesticides and other residues</strong> that display <strong>pH dependence in their recoveries</strong>, depending on the <strong>sample matrix</strong>.</p><h5><strong>Chemicals used in the EN 15662 method</strong></h5><ul><li><strong>Acetonitrile:</strong> Used to perform single-phase extraction on a sample</li><li><strong>Anhydrous MgSO₄:</strong> Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li><strong>NaCl:</strong> Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes residual water from sample</li><li><strong>Trisodium Citrate:</strong> Buffers the sample to stabilize pH</li><li><strong>Disodium Citrate Sesquihydrate:</strong> Buffers the sample to stabilize pH</li><li><strong>PSA:</strong> Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step</li><li><strong>C18:</strong> Removes fats, sterols, and other non-polar interferences from sample</li><li><strong>GCB:</strong> Graphitized carbon black, removes pigment (<i>not recommended for use with planar pesticides</i>)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_3_721562526f.jpg" alt="Thermo Scientific QuEChERS Sample Preparation Kits" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_3_721562526f.jpg 245w," sizes="100vw" width="1471" height="488"></p><div><h4><strong>QuEChERS clean-up kit for NY/T 1380-2007 method</strong></h4></div><div><p>The <strong>NY/T 1380-2007 method</strong> is <strong>recommended by China Ministry of Agriculture</strong> for the <strong>QuEChERS Method sample preparation</strong> of <strong>51 specific pesticide residues</strong> in <strong>fruits and vegetables</strong> with <strong>analysis by GC-MS</strong>.</p><h5><strong>Chemicals used in the NY/T 1380-2007 method</strong></h5><ul><li><strong>Anhydrous MgSO₄:</strong> Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li><strong>PSA:</strong> Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dSPE step</li><li><strong>C18:</strong> Removes fats, sterols, and other non-polar interferences from sample</li></ul></div></div></div></div></div></div></div></div></div></div></div></div>

Products from category Consumables focused on Sample Preparation - page 1

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