Phenomenex Strata X-AW Solid Phase Extraction (SPE)

Weak Anion Mixed-Mode Solid Phase Extraction (SPE)

A weak anion-exchange functionalized polymeric sorbent that allows for complete retention of acidic compounds with a pKa less than 5, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.

Phenomenex: Strata-X-AW SPE

• Recommended Use: Extraction of acidic compounds, particularly strong acids

Starting Method

For Acids with pKa ≤ 5

• Column: 1 mL Methanol
• Equilibrate: 1 mL Water, pH 6-7
• Load: Diluted Sample pH 6-7
• Wash 1: 1 mL 25 mM Ammonium Acetate Buffered, pH 6-7
• Wash 2: 1 mL Methanol
• Elute Any Acid: 2x 500 µL 5 % NH₄OH in Methanol
• Elute Weak Acids: 2x 500 µL 5 % Formic Acid in Methanol

Phenomenex: Strata X single tube

Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Available Formats

• 96-Well-Plate

Phenomenex: Strata X 96-well plate

• 1, 3, and 6mL Tubes

Phenomenex: Strata X 1, 3, and 6mL Tubes

• Giga Tubes (12-150mL Tubes)

Phenomenex: Strata X Giga Tubes (12-150mL Tubes)