<p><strong>Join us for a free two-day LECO Separation Science Workshop on September 23–24, 2026, at the </strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/companies/219"><strong>C³AL Laboratory</strong></a><strong> at Cal State LA. Registration is free, and meals and refreshments will be provided throughout the workshop.&nbsp;</strong></p><h4><strong>Registration</strong></h4><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://forms.cloud.microsoft/pages/responsepage.aspx?id=iYeNbqIuykW7qZP4_jUEVbznDB40eK1PgjPzhHyb__9URTFTTFA5UEEzQ1A4MUtFV0o4TEJTNlZCOS4u&amp;route=shorturl"><strong>Register HERE</strong></a></p><p class="cke-user_style-important"><strong>Space is limited, register today!</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_LECO_Separation_Science_Workshop_registration_332543a18e.jpg" alt="LECO: LECO Separation Science Workshop - QR code for registration" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_LECO_Separation_Science_Workshop_registration_332543a18e.jpg 156w," sizes="100vw" width="437" height="437"></p></blockquote><h4><strong>Hand-on Experience with the LECO Complex Chemical Composition Analysis Lab C<sup>3</sup>AL</strong></h4><ul><li>Work directly with LECO instruments in the C<sup>3</sup>AL Laboratory</li><li>Learn complete analytical workflows from sample to answer</li><li>Get expert guidance from LECO application scientists</li><li>Develop methods and solve real-world challenges</li><li>Collaborate, ask questions, and build your skills</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Cal_State_LA_The_LECO_Pegasus_BTX_system_in_Cal_State_LA_s_LECO_Complex_Chemical_Compositional_Analysis_Lab_enhances_research_and_student_training_in_advanced_chemical_analysis_cover_b739e727ca.jpg" alt="Cal State LA: The LECO Pegasus BTX system in Cal State LA’s LECO Complex Chemical Compositional Analysis Lab enhances research and student training in advanced chemical analysis" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Cal_State_LA_The_LECO_Pegasus_BTX_system_in_Cal_State_LA_s_LECO_Complex_Chemical_Compositional_Analysis_Lab_enhances_research_and_student_training_in_advanced_chemical_analysis_cover_b739e727ca.jpg 227w," sizes="100vw" width="1252" height="863"></p><p>The first day will focus on the <strong>fundamentals and theory</strong> of comprehensive two-dimensional gas chromatography (<strong>GC×GC</strong>), with an emphasis on understanding how GC×GC can enhance your research, solve complex analytical challenges, and support your laboratory or company goals.&nbsp;</p><p>The second day will be dedicated to <strong>hands-on activities</strong>, giving participants the opportunity to work directly with <strong>LECO instrumentation</strong>, learn <strong>practical analytical workflows</strong>, and <strong>analyze their own samples</strong> with guidance from LECO application scientists.&nbsp;</p><p>Whether you are new to GC×GC or looking to expand its use in your research or organization, this workshop offers a unique opportunity to learn, explore, and apply the technology in a practical setting. Space is limited, so register early!</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Cal_State_LA_LECO_QUADJET_G_Cx_GC_coupled_to_Pegasus_BT_4_D_TOM_MS_in_the_Complex_Chemical_Composition_Analysis_Lab_C_AL_077a14ae34/Cal-State-LA-LECO-QUADJET-GCxGC-coupled-to-Pegasus-BT-4D-TOM-MS-in-the-Complex-Chemical-Composition-Analysis-Lab-(CAL).jpg" alt="Cal State LA: LECO QUADJET GCxGC coupled to Pegasus BT 4D TOM MS in the Complex Chemical Composition Analysis Lab (C³AL)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Cal_State_LA_LECO_QUADJET_G_Cx_GC_coupled_to_Pegasus_BT_4_D_TOM_MS_in_the_Complex_Chemical_Composition_Analysis_Lab_C_AL_077a14ae34/thumbnail_Cal-State-LA-LECO-QUADJET-GCxGC-coupled-to-Pegasus-BT-4D-TOM-MS-in-the-Complex-Chemical-Composition-Analysis-Lab-(CAL).jpg 234w," sizes="100vw" width="1949" height="1299"></p>

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<div><div><section><div><div><div><div><div><div><p><strong>This study introduces a highly sensitive compound-specific isotope analysis method for identifying protein binders in aged paintings. Using direct inlet pyrolysis-GC-combustion-isotope ratio mass spectrometry (DIP-GC-C-IRMS), the method targets δ13C values of pyrolytic diketopiperazines and reduces the required sample size from milligrams to micrograms.</strong></p><p>Reference materials showed that diketopiperazine isotope signatures preserve the distinction between terrestrial and marine collagen sources. Analysis of two 16th-century Italian paintings yielded values consistent with terrestrial animal glue, demonstrating that these isotopic markers can survive aging and degradation and support minimally destructive analysis of cultural heritage objects.</p></div></div></div></div></div><div><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/ancham/article/98/29/21183/5211080/Pyrolysis-Compound-Specific-Isotope-Analysis-of?searchresult=1"><strong>Pyrolysis Compound-Specific Isotope Analysis of Proteins in Aged Paintings: Diketopiperazine δ13C Values as Collagen Source Indicators&nbsp;</strong></a></h4><p>Eugenia Geddes da Filicaia* ; David A. Peggie; Iain P. Kendall; Ian D. Bull; Richard P. Evershed</p><p>Anal. Chem. (2026) 98 (29): 21183–21196.</p><p>&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.analchem.5c07901">https://doi.org/10.1021/acs.analchem.5c07901</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><div><div><div><p>The complexities and challenges presented by the analysis of protein paint binders illustrate the need for novel methods of identification for these materials, in particular to distinguish between terrestrial and fish-animal glues. An alternative approach with great potential may be found in the neighboring field of archeology, where collagen in bones and teeth has been used as a palaeodietary indicator since the 1970s. Determination of bulk δ<sup>15</sup>N values may suggest the position of an organism in the respective food web, while δ<sup>13</sup>C values offer insight into their diet. (<a href="javascript:;">27−29</a>) Bulk δ<sup>13</sup>C and δ<sup>15</sup>N values of collagen are weighted averages of the δ<sup>13</sup>C and δ<sup>15</sup>N values of their constituent AAs, which reflect both dietary sources and metabolic processes. Serine, glycine, and alanine (Ser, Gly, and Ala) are part of the glucogenic AA group, which is biosynthesized from intermediates of glycolysis. As pyruvate is the end product of glycolysis, considered the catabolic pathway of carbohydrates, the isotopic signature of these AAs should reflect that of dietary carbohydrates. Aspartic acid and glutamic acid (Asp and Glu), asparagine (Asn), and Pro are part of the ketogenic AA group, originating from intermediates of the tricarboxylic acid (TCA) cycle. Due to their TCA cycle precursors, the isotopic signature of these AAs should reflect that of the whole diet, if biosynthesized <i>de novo</i>. Finally, Hyp and hydroxylysine cannot be incorporated into proteins from the diet and are formed after the polypeptides are synthesized, during posttranslational processing of collagen. Hydroxylysine is formed from lysine (Lys), whereas Hyp results from hydroxylation of Pro residues. (<a href="javascript:;">30</a>) Moreover, AAs in the “trophic group“ tend to display a large enrichment in <sup>15</sup>N with increasing trophic level, unlike those belonging to the “source group.“</p></div><div><p>Significant limitations therefore surround the interpretation of bulk stable isotope ratios, which, however, may be overcome by compound-specific isotope analysis (CSIA). Individual AA δ<sup>13</sup>C values have proven invaluable for distinguishing terrestrial from marine diets of our ancestors, (<a href="javascript:;">30−35</a>) while AA δ<sup>15</sup>N values are being increasingly used in archeological trophic position studies. (<a href="javascript:;">36−39</a>) Despite the similarity in target material, CSIA has never been extended to the analysis of animal glue, or any other protein, in paintings. Reasons for this are likely linked to the sample sizes required for standard protocols for GC-combustion-isotope ratio MS (GC-C-IRMS), which, however, as illustrated by recent studies, (<a href="javascript:;">40−42</a>) may be significantly reduced by undertaking direct inlet pyrolysis (DIP)-GC-C-IRMS. Here, this technique is applied to the analysis of proteins of paintings, in particular by targeting, for the first time, the stable carbon isotope composition of DKPs, protein pyrolysates. Having confirmed that DKPs reflect the isotopic signature of their constituent AAs, DKP δ<sup>13</sup>C values may be used to distinguish terrestrial from aquatic animal glue in aged paintings.</p><h4><strong>Materials and Methods</strong></h4><h5><strong>Instrumental Analyses</strong></h5><div><p>For elemental analysis (EA)-IRMS, GC-FID, and GC-C-IRMS methods, please see SI1. All chromatograms were replotted in RStudio. DIP-GC-MS was carried out on an <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=10&amp;search=7890A"><strong>Agilent Technologies 7890A GC</strong></a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=10&amp;search=5975C"><strong>Agilent Technologies 5975C MSD</strong></a> (Agilent Technologies, Santa Clara, CA, USA), a single quadrupole MS. The GC was equipped with a nonpolar fused-silica capillary column (30 m × 0.25 mm i.d. × 0.25 μm film thickness) with a BPX5 stationary phase (5% phenyl polysilphenylene-siloxane, SGE) and a TSP in the multimode inlet (MMI), operated in splitless mode. The initial TSP temperature was 50 <span>°</span>C, set to rise to 450 <span>°</span>C (900 <span>°</span>C min<sup>–1</sup>, 3 min isothermal hold), before cooling to 250 <span>°</span>C (25 <span>°</span>C min<sup>–1</sup>, 40 min isothermal hold). The oven temperature began at 40 <span>°</span>C, was increased to 200 <span>°</span>C (10 <span>°</span>C min<sup>–1</sup>), and then to 310 <span>°</span>C (6 <span>°</span>C min<sup>–1</sup>) with a final hold time of 20 min. He was used as a carrier gas at a constant flow (1.2 mL min<sup>–1</sup>).</p></div><div><p>The MS was set to acquire in full scan mode (<i>m</i>/<i>z</i> 50–650) at 0.2 scan s<sup>–1</sup>, and was operated at an EI potential of 70 eV with a solvent delay of 6.5 min. The MS transfer line temperature was set to 280 <span>°</span>C, the ion source temperature to 230 <span>°</span>C, and the quadrupole to 150 <span>°</span>C. Data acquisition was undertaken using Agilent Technologies Chemstation, and data processing with Agilent Technologies MassHunter software (version 10.0) and the AMDIS deconvolution software (NIST, version 2.66). Peaks were identified using a combination of mass spectral information, GC retention times (RTs), and comparison to the NIST mass spectral library (Mass Spectral Search Program version 2.0 and NIST 08 MS Library), published data sets, and NG databases. An <i>n</i>-alkane “ladder“ (with a characteristic bell-shaped peak profile) standard, containing C<sub>7</sub>–C<sub>40</sub> saturated alkanes (0.01 mg mL<sup>–1</sup> in <i>n</i>-hexane), was used to monitor instrument performance.</p></div><div><p>DIP-GC-QTOF-MS analysis was carried out on an <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=10&amp;search=7890B"><strong>Agilent Technologies 7890B GC</strong></a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=10&amp;search=7200"><strong>Agilent Technologies 7200 Accurate Mass QTOF MS</strong></a> (Agilent Technologies, Santa Clara, CA, USA). The same method was used, except that the MMI was held at 450 <span>°</span>C for 1 min only. The MS was set with an acquisition rate of 5 spectra s<sup>–1</sup>, an acquisition time of 200 ms spectrum<sup>–1</sup>, and an emission current of 2.1 μA. DIP-GC-C-IRMS analysis was carried out on an <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=10&amp;search=8890"><strong>Agilent Technologies 8890 GC</strong></a> coupled to an <strong>Elementar isoprime precisION IRMS</strong> via an Elementar GC5 combustion interface, using a combustion reactor containing CuO pellets and Ag wool (Elementar Analysensysteme GmbH, Hanau, Germany), maintained at 850 <span>°</span>C. The GC parameters were identical to those for DIP-GC-MS, except that the MMI, after being held at 450 <span>°</span>C for 3 min, was cooled to 300 <span>°</span>C (900 <span>°</span>C min<sup>–1</sup>, isothermal hold for 50 min). Additionally, the oven program was typically shortened by setting a lower maximum temperature (280 <span>°</span>C instead of 310 <span>°</span>C), where it was held for 10 min. He was used as a carrier gas at a constant flow (2 mL min<sup>–1</sup>).</p></div></div></div></div></div></div></section></div></div><h4><strong>Results and Discussion</strong></h4><h5><strong>Pyrolytic Marker Identification from DIP-GC-MS of Protein References</strong></h5><div><p>The thermal degradation of intact proteins has been shown to be a multistep reaction, (<a href="javascript:;">45</a>) where the formation of DKPs, occurring in the first degradation step (<i>T</i><sub>max</sub> = 325 <span>°</span>C), does not require high Py temperatures. (<a href="javascript:;">21</a>) Protein markers may therefore be detected through DIP-GC-MS, as testified by the analysis of a soapstone fragment from Qarmaaluit (18th–19th century CE). (<a href="javascript:;">46</a>) Nonetheless, given that this technique is not widely reported in the literature, modern protein references were analyzed by DIP-GC-MS, and pyrolytic profiles obtained were compared to published Py-GC-MS data. Studies have been undertaken with (<a href="javascript:;">22, 47, 48</a>) and without (<a href="javascript:;">22, 26</a>) TMAH, hence, both methods were carried out here. Results were scrutinized, in particular, to identify pyrolytic markers suitable for DIP-GC-C-IRMS analysis. Egg yolk, which presents a similar AA composition as egg white, was not examined, as its high fat content was deemed to interfere with the analysis.</p></div><div><p>In general, DIP-GC-MS analyses involving derivatization with TMAH, in particular for animal glues, featured many more compounds compared to the total ion chromatograms (TICs) obtained without derivatization (see <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/ancham/article-supplement/5211080/pdf/ac5c07901_si_001/">Figure S1</a> for G1, aged gelatin spread). In this case, GC-MS TICs exhibited better baseline separation with the most abundant peaks corresponding to the targeted DKPs. Further analysis of glue samples was therefore carried out without derivatization, as good baseline resolution is central to reliable compound-specific stable isotope determinations. (<a href="javascript:;">43</a>) Analysis of rabbit skin glues (GRS1 and GRS2) and isinglass glues (GI1 and GI2) yielded GC-MS TICs identical to each other. In accordance with the literature, (<a href="javascript:;">21, 22, 49</a>) as illustrated in <a href="javascript:;">Figure 1</a>a, they are dominated by peaks corresponding to diketodipyrrole (peak 8) and cyclo(Pro-Gly) (peak 10). Because of its relatively high polarity, or overloading, the latter produces a broad (fronting) peak. (<a href="javascript:;">50</a>)</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2026_98_29_21183_21196_Figure_1_Partial_DIP_GC_QTOF_MS_total_ion_chromatograms_TI_Cs_for_a_isinglass_GI_2_b_casein_C1_and_c_egg_white_EW_1_f4442d8498.jpg" alt="Anal. Chem. (2026) 98 (29): 21183–21196: Figure 1. Partial DIP-GC-QTOF-MS total ion chromatograms (TICs) for (a) isinglass, GI2, (b) casein, C1, and (c) egg white, EW1. Information on relevant DKPs (in purple) and other peaks (in pink) is listed in Table S1." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2026_98_29_21183_21196_Figure_1_Partial_DIP_GC_QTOF_MS_total_ion_chromatograms_TI_Cs_for_a_isinglass_GI_2_b_casein_C1_and_c_egg_white_EW_1_f4442d8498.jpg 127w," sizes="100vw" width="1500" height="1840"></p></div><h5><strong>DIP-GC-C-IRMS for Source Determination of Proteins in Paintings from The National Gallery</strong></h5><div><p>Ground layer samples from two Italian Renaissance paintings in the National Gallery collection, NG599 (Madonna of the Meadows) and NG6368 (Tanaquil), were analyzed by GC by White in the 1980s. (<a href="javascript:;">15</a>) The author reported AA profiles consistent with the presence of animal glues, which, for this purpose, would be expected to be of terrestrial origin. However, given the low proportion of Pro and Hyp in NG6368, White discussed a possible aquatic origin for this glue. Analysis was repeated with the aforementioned DIP techniques, revealing very similar profiles to those of the reference animal glues (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/ancham/article-supplement/5211080/pdf/ac5c07901_si_001/">Figure S6</a>). The protein binder in the ground layers of both paintings exhibited similar DKP stable carbon isotope signatures. In particular, δ<sup>13</sup>C<sub>Pro-Gly</sub> values were determined to be −18.4‰ (±0.1) and −17.6‰ (±0.1) for NG599 and NG6368, respectively. The δ<sup>13</sup>C<sub>Pro-Hyp</sub> values were found to equal −19.8‰ (±0.4) and −18.6‰ (±0.001), respectively.</p></div><div><p>It should be noted that the SD observed for these determinations is lower than what was reported for some of the reference binders discussed above. These errors are in line with those observed for standard GC-C-IRMS methods. As these paint fragments were in the form of very fine powder, typical of paint samples, these results support that sample inhomogeneities might be primarily responsible for wide SDs, rather than the DIP-GC-C-IRMS method itself. In the case of marine animal glues, more enriched δ<sup>13</sup>C<sub>DKP</sub> values would be expected than those observed here, especially for cyclo(Pro-Gly). Instead, the determined δ<sup>13</sup>C<sub>DKP</sub> values suggest a terrestrial origin for the glues in both paintings. Indeed, by plotting the values as described above, as shown in <a href="javascript:;">Figure 7</a>, both paintings fall extremely close to the reference rabbit skin glues. Hence, for NG599, these results are in agreement with the previous analysis by White (1984). However, regarding the origin of the binder from NG6368, these findings support a similar terrestrial origin, suggesting that a lower-than-average concentration of Pro and Hyp is not characteristic of fish glues.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2026_98_29_21183_21196_Figure_7_a_d13_C_Pro_Gly_values_for_the_ground_layers_from_paintings_NG_599_purple_star_and_NG_6368_pink_star_bb585e6957.jpg" alt="Anal. Chem. (2026) 98 (29): 21183–21196: Figure 7. (a) δ13CPro-Gly values for the ground layers from paintings NG599 (purple star) and NG6368 (pink star), against their δ13CPro-Hyp values. Modern references for marine (GI1 and GI2) and terrestrial (GRS1 and GRS2) animal glues are in gray. (b) Corresponding Δ13C(Pro-Gly)–(Pro-Hyp) values against their δ13CPro-Gly values. The error bars represent the SD on either side (±) of the value. The δ13C values have been corrected to take into account post-Industrial Revolution effects of burning fossil fuels by the addition of 1.2‰. (68) Instrumental precision was 0.3‰." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2026_98_29_21183_21196_Figure_7_a_d13_C_Pro_Gly_values_for_the_ground_layers_from_paintings_NG_599_purple_star_and_NG_6368_pink_star_bb585e6957.jpg 72w," sizes="100vw" width="625" height="1349"></p><p>Although a wider range of reference material needs to be analyzed before this method may be reliably used, these results indicate that DIP-GC-C-IRMS is able to differentiate between terrestrial and marine animal glues in painting samples. Given the challenges currently faced by this field to differentiate fish glues from other animal glues, this innovative technique might enable new information to be gathered on artist techniques and the history of use of these materials. It is important to note that the method can be applied to many other fields of research. In addition to painting analysis, this minimally invasive protocol is pertinent to any discipline that involves the analysis of degraded proteins, heterogeneous and complex matrices, or where access to samples is restricted due to ethical implications or lack of material. This includes environmental and forensic sciences, paleo- and zooarcheology, paleoecology, and anthropology.</p></div><h4><strong>Conclusions</strong></h4><div><p>Aged proteins encountered in painted cultural heritage have usually undergone extreme structural changes from the freshly applied material, often rendering unambiguous protein source determination unattainable. In particular, the distinction between terrestrial and aquatic animal glues is typically not possible without proteomics approaches. Nonetheless, this paper demonstrates that, with minimal sample sizes, DIP-GC-C-IRMS permits determination of δ<sup>13</sup>C values of DKPs arising from pyrolysis of aged proteinaceous material, providing the first record of the isotopic signature of these compounds. In this study, δ<sup>13</sup>C values of DKPs were shown to reflect the δ<sup>13</sup>C values of the parent AAs and, therefore, to permit unambiguous differentiation between terrestrial and marine animal glues, analogous to the use of CSIA of archeological collagen for palaeodietary reconstruction.</p></div><div><p>Remarkably, DIP-GC-C-IRMS, requiring a considerably smaller sample (in the microgram range) than typical GC-C-IRMS methods (in the milligram range), renders CSIA accessible for the investigation of proteinaceous material in painted cultural heritage for the first time. This widens the application of CSIA to precious, unique, and fragile objects in cultural heritage and beyond. Moreover, the rapidity of the analysis places DIP-GC-C-IRMS as a useful screening method to identify materials of interest for further proteomic analyses.</p></div><div><p>First, the isotopic imprint of modern references for rabbit skin glues, isinglass glues, casein, chicken, and duck eggs was confirmed through established EA-IRMS and GC-C-IRMS methods. As expected, bulk and individual AA δ<sup>13</sup>C and δ<sup>15</sup>N values for terrestrial animal glues exhibited a higher depletion in <sup>13</sup>C and <sup>15</sup>N than the marine glues. The δ<sup>13</sup>C values of DKPs arising from DIP-GC-C-IRMS of the same materials were found to retain this relationship. Furthermore, the ground layers of two 16th-century Italian paintings from the National Gallery collection, known to contain animal glue, yielded δ<sup>13</sup>C<sub>DKP</sub> values consistent with the terrestrial variety, confirming that the isotopic signature of these compounds is retained through aging and degradation.</p></div>

Pyrolysis Compound-Specific Isotope Analysis of Proteins in Aged Paintings: Diketopiperazine δ13C Values as Collagen Source Indicators

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EST Analytical FLEX 2 Robotic Sampling Platform

The FLEX 2 autosampler streamlines GC/GC-MS workflows with liquid, headspace, and SPME capability, MTX tool change, and Prep Ahead for faster, more reliable VOC analysis.

<p><strong>The FLEX 2 is a versatile GC autosampler designed to handle both sample preparation and sample introduction for GC and GC/MS analysis. Supporting liquid injection, headspace, and SPME, it delivers exceptional accuracy and reproducibility. Its Prep Ahead function improves efficiency by allowing sample preparation during ongoing GC runs, maximizing system utilization and throughput.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/EST_Analytical_FLEX_2_Robotic_Sampling_Platform_3_853e02f151.jpg" alt="EST Analytical: FLEX 2 Robotic Sampling Platform" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_EST_Analytical_FLEX_2_Robotic_Sampling_Platform_3_853e02f151.jpg 168w," sizes="100vw" width="628" height="584"></p><h4><strong>Key Features</strong></h4><h5><strong>Flexible Sample Preparation with Multi Tool Change (MTX)</strong></h5><ul><li>Automated <strong>Multi Tool Change (MTX)</strong> enables seamless switching between tools</li><li>Enhances laboratory productivity, accuracy, and safety by reducing manual prep errors</li><li>Supports dilution, reagent addition, and internal standard spiking with precision</li><li>Can operate as a benchtop preparation station for derivatization and extraction steps</li></ul><h5><strong>Liquid Injection Capabilities</strong></h5><ul><li>Injection range: <strong>0.5–10 μL</strong> (standard syringe) or <strong>50–500 μL</strong> (optional large-volume syringe)</li><li>Supports both standard and large-volume injections (up to 500 μL)</li><li>Programmable injection speed control, from ultra-fast to very slow</li><li>Optional cooled trays available for temperature-sensitive samples</li></ul><h5><strong>Headspace Sampling</strong></h5><ul><li>Injection range: <strong>100–2500 μL</strong> (2.5 mL syringe), <strong>100–1000 μL</strong> (1.0 mL syringe), or <strong>500–5000 μL</strong> (5.0 mL syringe)</li><li>Offers both <strong>orbital</strong> and <strong>oscillating</strong> agitation to improve sample consistency</li><li>Six heated vial positions for <strong>2, 10, or 20 mL vials</strong></li><li>Temperature range: <strong>ambient to 200 °C</strong></li><li>Shaking: orbital (120–750 rpm) or oscillation (190 Hz)</li></ul><h5><strong>Automated SPME</strong></h5><ul><li>Compatible with both traditional and direct immersion SPME methods</li><li>Heated conditioning station:<ul><li>Fiber: <strong>ambient to 350 °C</strong></li><li>Needle: <strong>ambient to 150 °C</strong></li><li>Sweep gas port for conditioning</li></ul></li><li>Heated mixing station: <strong>ambient to 150 °C</strong>, mixing speeds <strong>380–725 rpm</strong></li></ul><h5><strong>Efficiency &amp; Throughput</strong></h5><ul><li><strong>Prep Ahead function</strong> allows ongoing sample prep during GC runs</li><li>Fully automates switching between liquid injection, headspace, and SPME with MTX</li><li>Reduces manual workload and preparation errors while improving reproducibility</li></ul><h4><strong>Technical Specifications</strong></h4><ul><li><strong>Compatible Systems</strong>: GC and GC/MS platforms</li><li><strong>Injection Modes</strong>: Liquid (0.5–500 μL), Headspace (100–5000 μL), SPME (traditional &amp; direct immersion)</li><li><strong>Temperature Ranges</strong>: Headspace up to 200 °C, SPME fiber up to 350 °C, SPME needle up to 150 °C</li><li><strong>Agitation</strong>: Orbital (120–750 rpm) and oscillating (190 Hz)</li><li><strong>Capacity</strong>: Six heated vial positions for 2, 10, or 20 mL vials</li><li><strong>Automation</strong>: Multi Tool Change (MTX), Prep Ahead function</li></ul>

EST Analytical LGX-50 for Methane in Water

The EST LGX-50 automates RSK-175 dissolved gas testing in water, boosting throughput, ensuring ASTM/EPA compliance, and reducing manual sample preparation errors.

<p><strong>The LGX-50 automates the preparation of water samples for headspace analysis of dissolved gases such as methane, ethane, and ethene. Traditionally, RSK-175 testing has been labor-intensive and time-consuming, requiring manual vial preparation. The LGX-50 eliminates this step, streamlining workflows and ensuring compliance with EPA Method 5035 and ASTM D8028. By automating dissolved gas extraction, laboratories can boost throughput, reduce errors, and deliver more consistent results for shale gas and environmental testing.</strong></p><h4><strong>Key Features</strong></h4><h5><strong>Maximized Productivity</strong></h5><ul><li>Fully automates <strong>RSK-175 sample processing</strong></li><li>ASTM D8028 compliant for dissolved gas testing</li><li>Holds up to <strong>50 samples</strong> for high-throughput operation</li><li>Optical sensors prevent vial misplacement and syringe/elevator errors</li><li>Robust XYZ design ensures reliability and consistent performance</li></ul><h5><strong>Simplified Operation</strong></h5><ul><li>Intuitive, user-friendly software designed for quick setup and operation</li><li>Remote access functionality allows labs to monitor sample progress via the server</li><li>Reduces manual preparation steps, increasing accuracy and reproducibility</li></ul><h4><strong>Technical Specifications</strong></h4><ul><li><strong>Compliance</strong>: EPA Method 5035, ASTM D8028</li><li><strong>Capacity</strong>: Up to 50 samples per batch</li><li><strong>Target Compounds</strong>: Methane, ethane, ethene, and other dissolved gases in water</li><li><strong>Automation</strong>: XYZ robotic design with optical sensors for error prevention</li><li><strong>Software</strong>: Intuitive, remote-access capable interface</li></ul>

Thermo Scientific ITEX Dynamic Headspace Tool 

Thermo Scientific ITEX Dynamic Headspace Tool enables automated VOC enrichment with ppt sensitivity using dynamic headspace for GC workflows.

<p><strong>The Thermo Scientific™ ITEX Dynamic Headspace Tool is an autosampler accessory for the</strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/63"><strong> TriPlus™ RSH </strong></a><strong>and </strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/422"><strong>TriPlus RSH SMART</strong></a><strong> Autosamplers designed for the enrichment of volatile organic compounds (VOCs) using dynamic headspace technology. It enables ppt-level sensitivity and allows sample enrichment to be easily integrated into an automated gas chromatography (GC) workflow.</strong></p><ul><li>The ITEX-DHS technique uses a gas-tight syringe to collect the headspace and a microtrap inside the syringe needle to efficiently trap and concentrate VOCs through repeated syringe strokes.&nbsp;</li><li>Sensitivity can be scaled according to the number of strokes, making the method suitable for both low- and high-concentration samples.&nbsp;</li><li>The system is designed for easy method set-up with only a few parameters, simple access to the trap, and excellent robustness for long-term operation.&nbsp;</li><li>A closed system removes any risk of breakthrough.&nbsp;</li><li>Independent trap desorption temperature and flow control maximize desorption efficiency without affecting GC inlet settings.&nbsp;</li><li>When used with the TriPlus RSH SMART autosampler, the SMART gas-tight syringe enables automatic usage traceability.&nbsp;</li><li>The entire automated workflow can be fully controlled through <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/76"><strong>Thermo Scientific™ Chromeleon™ CDS</strong></a>.</li></ul><p>For VOC analysis in many liquid and solid samples, ITEX offers a valuable alternative to classical enrichment techniques by combining ease of use with robust performance.</p><h3><strong>Key features</strong></h3><ul><li>Dynamic headspace VOC enrichment with<strong> ppt-level sensitivity</strong></li><li><strong>Easy integration</strong> into automated GC workflows</li><li><strong>Gas-tight syringe and microtrap</strong> in the needle for efficient analyte concentration</li><li><strong>Scalable sensitivity</strong> based on the number of syringe strokes</li><li><strong>Easy method set-up</strong> with few parameters</li><li><strong>Robust design </strong>for long-term operation</li><li><strong>Closed system</strong> minimizing the risk of breakthrough</li><li><strong>Independent desorption</strong> temperature and flow control</li><li><strong>Automatic usage</strong> traceability with SMART gas-tight syringe</li><li>Full control through <strong>Chromeleon CDS</strong></li></ul><h3><strong>Technical specifications</strong></h3><ul><li><strong>Description: </strong>includes SMART gas-tight syringe, focusing trap, built-in trap heating, cooling fan, and trap cleaning capability</li><li><strong>Application: </strong>enrichment of volatile organic compounds (VOCs)</li><li><strong>Compatible equipment: </strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/63"><strong>TriPlus RSH</strong></a> and <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/422"><strong>TriPlus RSH SMART </strong></a>Autosamplers</li><li><strong>Type:</strong> autosampler accessory</li><li><strong>Unit size: </strong>each</li></ul>

Thermo Scientific TriPlus™ RSH SMART Autosampler

Extend automation, performance, and versatility for GC and GC/MS applications, while ensuring easier and safer operations through SMART consumables ID recognition and usage tracking.

<div><div id="product-description"><p><strong>Expand automated sampling capabilities beyond liquid, headspace, and solid phase microextraction (SPME) injections. The advanced, built-in robotics of the Thermo Scientific™ TriPlus™ RSH SMART Autosampler deliver exceptional precision, sample handling flexibility, and reliable unattended operation. Enabling SMART consumables tracking, it offers easier, safer and traceable operations. Accurate and repeatable sample preparation and injection are essential to achieving the highest quality GC/GC-MS results. Enhance data quality and laboratory efficiency by automating your entire sample handling workflow.</strong></p></div><div><p>The TriPlus RSH SMART Autosampler brings together technological innovations that further expand automation, performance and versatility for GC and GC-MS applications, while ensuring easier and safer operations through SMART consumables ID recognition and usage tracking. Automated high-precision sample handling eliminates the manual labor and downtime typically required for tasks as standard/sample dilutions, internal standard addition and derivatization.</p><h3><strong>SMART Consumables</strong></h3><p>Validated SMART consumables are equipped with unique SMART chip storing parameters, ranges and usage history; enabling full traceability of sample preparation down to the syringe.</p><h3><strong>Automatic Syringe Changeout</strong></h3><p>Automatic Tool Change (ATC) capability automates the use of different syringes for sample preparation steps or different injection techniques within the sequence.</p><h3><strong>Vial Bottom Sensing Expands Application Range</strong></h3><p>Precise detection of vial bottoms enables the autosampler to accurately withdraw from sample amounts as little as 5 μL, enhancing data quality of sample-limited applications.</p><h3><strong>Flexible liquid sample handling</strong></h3><p>Optimized liquid injection modes and customizable syringe filling techniques, support a wide range of sample types and full GC inlet compatibility.</p><h3><strong>Automated headspace injection for volatile analysis</strong></h3><p>High temperature gas-tight syringe allows direct headspace injection into the GC inlet for increased injection volume flexibility. Sample vial capacity of up to 300x 10 mL/20 mL vials and sample incubation overlapping, facilitate long unattended operation.</p><h3><strong>Automated, solvent-free SPME</strong></h3><p>Precise control of all steps—fiber preconditioning, adsorption and desorption, as well as fiber washes—delivers maximum performance for SPME applications. Boost productivity with the optimized SPME Arrow design. It offers optimized fiber geometry to achieve 10x superior sensitivity compared to classical SPME, 2x faster extraction time and superior robustness.</p><h3><strong>Automated Sample Preparation</strong></h3><p>The Triplus RSH SMART Autosampler combines highly versatile sample injection capability with automated sample preparation procedures, eliminating typical GC-MS workflow bottlenecks. Daily operations ranging from sample/standard dilution to more complex derivatization protocols, can be fully automated and easily programmed with the Sampling Workflow Editor Software.</p><h3><strong>Extend productivity with the optional 1D barcode reader</strong></h3><p>The optional 1D barcode reader eliminates the need to log and track sample information manually; enabling you to streamline internal operations, save time, increase efficiency, and reduce errors.</p><h3><strong>Specifications</strong></h3><ul><li><strong>Type: </strong>TriPlus RSH SMART Standard Liquid, Regular Rail</li><li><strong>Sample Capacity:</strong> 4608 Well Plates, 840 x 0.5 mL or 0.7 mL Vials, 648 x 2 mL Sample Vials, 240 x 10 mL or 20 mL Vials</li><li><strong>Injection Mode: </strong>8 Fully Customizable Method-Specific Injection Mode Presets</li><li><strong>Injection Speed:</strong> Selectable from 0.1 μL/s to 2000 μL/s</li><li><strong>Injection Volume: </strong>0.1 to 10,000 μL</li><li><strong>Description: </strong>TriPlus RSH SMART configured for liquid injection on regular X-axis length. Only for manual tool change. Complete with liquid syringe tool, 2 x 10 μL SMART syringes, standard washing station and a single tray holder with 3 trays for 2 mL vials. Expandible to other sampling techniques (Headspace, SPME, SPME Arrow, ITEX-DHS) and with sample preparation tools.</li><li><strong>Tool Exchange:</strong> Only for manual tool exchange. No tool park station.</li><li><strong>For Use With (Equipment):</strong> Compatible with Smart syringes/fibers only.</li></ul></div></div>

Thermo Scientific™ TriPlus™ RSH Autosampler

Robotic GC autosampler with multimode injection, tool exchange, and full sample prep automation. Enables 24/7 workflows for food, environmental, and forensic labs.

<h4><strong>Flexible, high-capacity autosampler for GC and GC-MS with advanced sample prep automation</strong></h4><p><strong>The TriPlus RSH Autosampler is a versatile robotic sampling platform designed to maximize automation, precision, and productivity in gas chromatography workflows. Fully compatible with Thermo Scientific™ GC and GC-MS systems, it supports liquid, headspace (HS), and SPME/SPME Arrow injections, with optional modules for µSPE, ITEX-DHS, and automated sample preparation. The unique Automatic Tool Change (ATC) system allows the autosampler to switch between up to six syringes in a single sequence, enabling unattended workflows for complex multistep protocols.</strong></p><h3><strong>Key Features and Benefits</strong></h3><h4>🧪 <strong>Multimode Injection and Sample Handling</strong></h4><ul><li>Supports liquid, headspace, SPME, SPME Arrow, and ITEX-DHS techniques</li><li>Switch between injection modes in a single sequence using ATC</li><li>Bottom vial sensing enables precise injections from as little as <strong>5 μL</strong> of sample</li></ul><h4>🔁 <strong>Automated Sample Preparation</strong></h4><ul><li>Perform dilution, standard addition, derivatization, and internal standard workflows</li><li>Tools: Vortexer, Incubator, Heatex-Stirrer, Dilutor, µSPE module</li><li>Visual drag-and-drop <strong>Sampling Workflow Editor</strong> for custom prep protocols</li></ul><h4>📦 <strong>High Throughput and Capacity</strong></h4><ul><li>Unattended operation with up to <strong>972 × 2 mL vials</strong> or <strong>840 × 0.7 mL vials</strong></li><li>Extended X-arm option for larger trays and accessory support</li><li>Dual-GC configurations with Clone Mode or DoublePro setups</li></ul><h4>⚙️ <strong>Precision and Flexibility</strong></h4><ul><li>Automated Tool Change (ATC): Up to <strong>6 tools</strong> in one sequence</li><li>Injection volume range: <strong>0.1 μL to 10 mL</strong></li><li>Compatible with all major GC injector types (SSL, PTV, OC, PKD, PPKD)</li></ul><h4>🧠 <strong>Smart Software and Control</strong></h4><ul><li>Fully integrated with <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/76"><strong>Chromeleon™</strong></a>,<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758"><strong> Xcalibur™</strong></a>, and <strong>Chrom-Card™ CDS</strong></li><li>Local controller or virtual terminal control interface</li><li>Barcode readers for full sample tracking</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/application::product.product/Thermo-TriPlus-RSH-Autosampler-1.jpg" alt="Thermo Scientific™ TriPlus™ RSH Autosampler" width="null" height="null"></p><h3><strong>Applications</strong></h3><ul><li><strong>Food safety</strong>: Trace analytes, contaminants, derivatization (e.g. FAMEs, dioxins)</li><li><strong>Environmental</strong>: VOCs, SVOCs, POPs, air toxics</li><li><strong>Forensics &amp; clinical</strong>: Biological matrices, explosive residues, drugs</li><li><strong>Chemical industry</strong>: Routine QC, trace impurities, residual solvents</li><li><strong>High-throughput labs</strong>: 24/7 unattended workflows with complex prep steps</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/application::product.product/QExactive-GCMSMS-Orbitrap-with-Autosampler-sideangle-1856x1391.jpg-650.jpg" alt="Thermo Scientific™ TriPlus™ RSH Autosampler" width="null" height="null"></p>

Thermo Scientific™ TriPlus™ 500 GC Headspace Autosampler

Powered by a completely new design, the Thermo Scientific™ TriPlus™ 500 Headspace Autosampler delivers reliability and robustness for testing laboratories performing volatiles analysis.

<p><strong>Powered by a completely new design, the Thermo Scientific™ TriPlus™ 500 Headspace Autosampler delivers reliability and robustness for testing laboratories performing volatiles analysis.</strong></p><p>Valve-and-loop technology and <strong>other innovative features</strong> ensure that this autosampler streamlines daily workflows, saving time with reliable unattended operation, optimizing sample throughput with expanded vial capability, and facilitating method portability with powerful software. Smartly integrated with the <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/483"><strong>Thermo Scientific™ TRACE™ 1600 Series GC</strong></a><strong>,</strong> this modular platform provides today's analytical testing and services laboratories with the productivity needed to succeed.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Schematicke_znazorneni_nastriku_pomoci_Head_Space_autosampleru_Thermo_Tri_Plus_500_fd64009b8a/Pragolab-Schematicke-znazorneni-nastriku-pomoci-HeadSpace-autosampleru-Thermo-TriPlus-500.jpg" alt="Thermo Scientific™ TriPlus™ 500 GC Headspace Autosampler: Valve-and-loop headspace injection principle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Schematicke_znazorneni_nastriku_pomoci_Head_Space_autosampleru_Thermo_Tri_Plus_500_fd64009b8a/thumbnail_Pragolab-Schematicke-znazorneni-nastriku-pomoci-HeadSpace-autosampleru-Thermo-TriPlus-500.jpg 245w," sizes="100vw" width="1812" height="560"></p><h4><strong>Reliability for analytical testing and services laboratories and versatility for research and method development.</strong></h4><p>The new design of the TriPlus 500 Headspace Autosampler incorporates innovative features for highly accurate analytical results, including:</p><h5><strong>New pneumatic controls for high precision and robustness</strong></h5><ul><li><strong>Best injection repeatability</strong> for consistent results at every run.</li><li><strong>No carryover </strong>due to effective sample path purging.</li><li><strong>Automatic leak check </strong>during injection to ensure safe runs.</li></ul><figure class="image"><img style="aspect-ratio:681/480;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Tri_Plus_500_GC_Headspace_Autosampler_2_62a58c525f.jpg" alt="Thermo Scientific™ TriPlus™ 500 GC Headspace Autosampler: Long Sequence Area Counts Repeatability. 120 consecutive headspace injections of a 50 ppm ethanol solution in water (5 ml in 10 ml crimp top vial). Overall RSD%=0.7" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Tri_Plus_500_GC_Headspace_Autosampler_2_62a58c525f.jpg 221w," sizes="100vw" width="681" height="480"></figure><h5><strong>Best sample integrity and ease-of-use via direct column connection</strong></h5><ul><li><strong>A shorter sample path</strong> for high inertness towards polar compounds.</li><li><strong>Expanded high temperature</strong> <strong>applicability</strong> <strong>with higher recovery </strong>of high boiling point compounds.</li><li><strong>Simplified method set up </strong>with no transfer line and injector temperature settings required.</li></ul><figure class="image"><img style="aspect-ratio:720/484;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Tri_Plus_500_GC_Headspace_Autosampler_3_a7e8932898.jpg" alt="Thermo Scientific™ TriPlus™ 500 GC Headspace Autosampler: Recovery of a standard hydrocarbon mixture C10-C40 at high temperature operation, showing >60% recovery at 300°C" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Tri_Plus_500_GC_Headspace_Autosampler_3_a7e8932898.jpg 232w," sizes="100vw" width="720" height="484"></figure><h5><strong>Compact yet scalable design for better laboratory efficiency and productivity</strong></h5><ul><li>Compact HS-GC design saves<strong> 30%</strong> of valuable bench space.</li><li>Tailored throughput requirements with in-field scalability from<strong> 12 to 240 vials</strong>, requiring no additional space and no compromise on performance.</li><li>Faster sample equilibration with the new <strong>Quick Spin Shaking design</strong> for maximal sensitivity in a shorter cycle time.</li></ul><h5><strong>Comprehensive HS-GC configuration solution</strong></h5><ul><li>Available with<strong> direct column connection</strong> or transfer line for maximum configuration flexibility.</li><li>When a direct column connection is not possible, the autosampler configured with a transfer line maintains <strong>high precision, efficient sample transfer.</strong></li><li>When configured with a transfer line, the TriPlus 500 HS autosampler<strong> </strong>is ideal for connecting to the <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/483"><strong>Thermo Scientific™ TRACE™ 1600 Series GC</strong> </a>with auxiliary oven or for connection to third-party GC instruments</li><li>The TriPlus 500 HS with transfer line allows <strong>combined headspace and liquid injection</strong> in the same column, through operation with the <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/70"><strong>TriPlus 100 LS </strong></a><strong>or TriPlus RSH or</strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/422"><strong> RSH SMART</strong></a><strong> autosampler on the same GC.</strong></li></ul><h5><strong>Software and system checks for compliance and data integrity</strong></h5><ul><li>Integrated with <strong>Thermo Scientific™ Chromeleon™ CDS software</strong>, Title 21 CFR Part 11-compliant.</li><li>Easy analytical system performance qualification with the IQ/OQ/PQ Qualification Wizard and daily sequence performance checks with the System Suitability Test Wizard.</li></ul><h4><strong data-start="0" data-end="18" data-is-only-node="">Specifications</strong></h4><ul><li><strong data-start="21" data-end="30">Type:</strong> TriPlus™ 500 GC Headspace Autosampler</li><li><strong data-start="71" data-end="91">Sample Capacity:</strong> 12 Vials, Upgradable to 120 and 240 Vials</li><li><strong data-start="136" data-end="155">Injection Mode:</strong>&nbsp;<ul><li>Standard</li><li>Multiple Headspace Extraction (MHE)</li><li>Multiple Headspace Injection (MHI)</li></ul></li><li><strong data-start="240" data-end="256">Description:</strong> TriPlus™ 500 Headspace Autosampler with Direct Column Connection</li><li><strong data-start="324" data-end="337">Software:</strong> Thermo Scientific™ Chromeleon CDS, Thermo Scientific™ TraceFinder, and Thermo Scientific™ XCalibur Software</li><li><strong data-start="448" data-end="477">For Use With (Equipment):</strong> <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/483">Trace 1600 Series</a></li><li><strong data-start="498" data-end="511">Quantity:</strong> Each</li><li><strong data-start="519" data-end="533">Unit Size:</strong> Each</li></ul>

LECO L-PAL3 GC Autosampler

LECO L-PAL3 GC Autosampler delivers flexible, discrimination-free GC injections with automated liquid, headspace, SPME, and ITEX sampling, high vial capacity, and minimal carryover.

<p><strong>Available exclusively from LECO, the L-PAL3 GC Autosampler is a highly robust, fully integrated autosampling platform designed for high flexibility and reliability in gas chromatography workflows. Based on proven CTC PAL3 technology, it supports discrimination-free ultrafast GC injections and minimizes carryover while ensuring precise and reproducible sample handling.</strong></p><p>Key innovations include <strong>automatic tool exchange (ATX)</strong> for seamless switching between liquid, headspace, SPME, and ITEX sampling modes, <strong>vial bottom sensing</strong> to prevent mis-injections, and high vial capacity for unattended operation. These features allow analysts to focus on compound identification without downtime or uncertainty.</p><h3><strong>Key Features</strong></h3><ul><li>Allows for automation of up to 162 two mL samples, or 45 ten/twenty mL samples.</li><li>Discrimination-free injection speeds with complete injection cycle time down to 100 milliseconds.</li><li>Bottom-sensing technology ensures that even very small volumes can be sampled.</li><li>Four separate models available so you may configure your package based on your autosampler needs.</li><li>Automatic Tool Exchange (ATX) allows users to switch between liquid, SPME, and headspace seamlessly.</li><li>96 to 384 well plate sampling supported; requires foil cutter.</li><li>Now available with Peltier cooled racks.</li></ul><h3><strong>Available Configurations</strong></h3><h4><strong>L-PAL3 GC Autosampler – Liquid Injections</strong></h4><ul><li>3-axis CTC-based autosampler/injector</li><li>Standard liquid injections</li><li>5-position solvent wash station</li><li>Liquid syringe tool</li><li>GC mounting kit</li><li>Single tray holder with <strong>3 trays × 54 positions (2 mL vials)</strong></li><li><strong>Non-upgradable configuration</strong></li></ul><h4><strong>L-PAL3 GC Autosampler – Liquid &amp; Headspace Injections</strong></h4><ul><li>Liquid and static headspace injections</li><li>Heated agitator</li><li>5-position solvent wash station</li><li>Liquid syringe tool + heated headspace syringe tool</li><li>GC mounting kit</li><li>Two tray holders:<ul><li><strong>3 × 54 positions (2 mL vials)</strong></li><li><strong>3 × 15 positions (10/20 mL vials)</strong></li></ul></li><li><strong>Manual tool exchange</strong> between liquid and headspace modes</li></ul><h4><strong>ITEX Dynamic Headspace Tool</strong></h4><ul><li>Rapid, sensitive enrichment of volatile and semi-volatile compounds</li><li>Suitable for solid, liquid, and gaseous samples</li><li>Choice of adsorbents for optimal sensitivity</li><li>No sample loops, transfer lines, or valves → minimal contamination risk</li><li>ATX enables <strong>Headspace, SPME, and ITEX</strong> within a single sequence</li></ul><h4><strong>L-PAL3 GC Autosampler – Liquid &amp; SPME Injections</strong></h4><ul><li>Liquid injections and all SPME modes (immersion, headspace)</li><li>Heated agitator</li><li>5-position solvent wash station</li><li>Liquid syringe tool + SPME fiber tools</li><li>SPME fiber conditioning station</li><li>GC mounting kit</li><li>Two tray holders:<ul><li><strong>3 × 54 positions (2 mL vials)</strong></li><li><strong>3 × 15 positions (10/20 mL vials)</strong></li></ul></li><li><strong>Manual tool exchange</strong> between liquid and SPME modes</li></ul><h3><strong>L-PAL3 GC Autosampler – Liquid, Headspace &amp; SPME</strong></h3><ul><li>Fully automated switching between liquid, headspace, and SPME</li><li>Heated agitator</li><li>5-position solvent wash station</li><li>Tools for liquid syringe, headspace syringe, and SPME fibers</li><li>SPME fiber conditioning station</li><li>GC mounting kit</li><li>Two tray holders:<ul><li><strong>3 × 54 positions (2 mL vials)</strong></li><li><strong>3 × 15 positions (10/20 mL vials)</strong></li></ul></li></ul>

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