Clarity Control Module HT800L

Importance of HTA HT800L Autosampler Control

The integration of the HTA HT800L autosampler into a chromatography station via the Clarity Control Module significantly enhances sample throughput, method reproducibility and data traceability. Automated control over injection, wash and reagent addition steps minimizes manual handling errors and supports high‐throughput workflows in QA/QC, research and industrial analysis.

Goals and Overview

This document outlines the configuration, operation and reporting of the HT800L autosampler under Clarity software. It describes hardware requirements, serial communication setup, method parameterization and real‐time device monitoring. The objective is to enable analysts to fully automate sample preparation and injection sequences within a unified chromatography environment.

Instrumental Setup

• HTA HT800L autosampler with syringe volumes from 250 µL to 5 mL and loop sizes from 2 µL to 1 mL.
• Clarity software with AS Control Module (p/n A26) on a PC with free serial COM port or MultiCOM adapter.
• Standard DB9F‐DB9M RS-232 cable (p/n SK02) for direct serial control (firmware ≥ 1.07 required).
• Optional cooling module for tray temperature conditioning.

Methodology and Configuration

• Hardware Wiring: serial link between PC and autosampler; optional integration with other HPLC modules for status and start signals.
• Clarity System Configuration: adding HT800L driver, assigning COM port, naming the device and setting ready/start signal polarity.
• Method Setup Sub-Tabs:
  • Injection: mode selection (full/partial loop or pick-up µL), air-gap, fill speed, viscosity delay and flush volume.
  • Move: quantized transfers with sample dilution or cleanup, volume and wash settings.
  • Wash: pre- and post-inject wash and purge cycles with repetition and volume controls.
  • Reagent: up to three reagents can be added with volume, repetition, mixing and reaction wait time.
  • Positions: mapping of vials A–J for sample, reagents, washes and transfer buffers.
• Device Monitor: live status of tray door, cooling, manual wash operations and wash parameters.
• Report Integration: inclusion of all AS method parameters in Clarity reports under the Injection Control option.

Main Results and Discussion

Automation allows precise fluid handling routines, including programmable multi-step reagent additions, mixing cycles and dilution protocols. The HT800L’s quantized and pick-up injection modes support flexible sample preparation. Comprehensive wash sequences reduce carry-over, while tray cooling extends sample integrity during extended runs. Real-time monitoring and method sending before each run ensure synchronized operation with chromatographic analysis.

Practical Benefits and Applications

• High reproducibility of injection volumes and timing.
• Reduced manual labor and lower risk of cross-contamination.
• Scalable protocols for sample dilution, derivatization or cleanup.
• Seamless reporting and method archiving within the Clarity environment.

Future Trends and Opportunities

• Enhanced digital interfaces (e.g. USB, Ethernet) for faster data exchange and remote control.
• Integration with cloud-based LIMS and data analytics platforms.
• AI-driven method optimization for rapid protocol development.
• Miniaturized and multi-channel autosamplers for multiplexed applications.

Conclusion

The HTA HT800L autosampler, when fully integrated into Clarity, provides a robust and flexible solution for automated sample handling in chromatographic workflows. Its configurable injection, wash and reagent modules enable a broad range of analytical protocols with improved throughput, accuracy and traceability.

References

No external references were provided.