Biotage PRESSURE+ 96 (User Manual)

Importance of the Topic

Positive‐pressure solid‐phase extraction (SPE) in a 96‐well format is a critical technique in modern analytical laboratories. It enables high‐throughput sample cleanup and concentration for environmental, food, pharmaceutical, and bioanalytical applications. The PRESSURE+ 96 system addresses the growing need for reproducible, efficient, and safe sample processing under controlled pressure, improving consistency across multi‐sample workflows and reducing turnaround times.

Objectives and Overview

The primary objective of the PRESSURE+ 96 user manual is to guide trained laboratory personnel through safe installation, operation, and maintenance of the positive‐pressure manifold. Key sections cover safety requirements, site preparation, step‐by‐step operating procedures, gas flow control, and routine cleaning and component replacement. The manual ensures compliance with CE and Machinery Directives, and supports effective use of all compatible 96‐well extraction plates and accessories.

Methodology

The system applies regulated compressed gas (air or nitrogen) to drive liquid through SPE sorbent beds in 96‐well plates. Two flow pathways are provided:

• Adjustable Flow (0–15 psi): A precision rotometer and needle valve restrict flow to 0–1,180 mL/min for delicate conditioning, sample loading, and elution.
• Maximum Flow (0–100 psi): A separate regulator delivers higher pressure for rapid washing, drying, or processing viscous or particulate‐laden samples.

Users assemble extraction and collection plates on a sliding platform, lower the manifold via dual rocker switches to compress the seal, and control gas flow via a three‐position switch (Adjustable, Maximum, Off). Protocol steps include conditioning, sample loading, washing, drying, elution, and decompression.

Instrumentation

The PRESSURE+ 96 hardware consists of:

• Positive‐pressure manifold with anodized aluminum, stainless steel, and plastic components
• Adjustable regulator, rotometer, and needle valve for low‐flow control
• Maximum Flow regulator for high‐pressure applications
• Sliding platform with handgrip and dual rocker switches for safe manifold movement
• Gas supply adaptor kit (polyethylene tubing, NPT fittings)
• Sealing gaskets and accessories for 96‐well plate compatibility

Key Results and Discussion

The system reliably delivers:

• Consistent flow rates at 25 psi (approx. 354 ± 24 mL/min per well, no column)
• Precise control for low‐flow applications to enhance reproducibility of analyte recovery
• High‐pressure capability up to 100 psi for rapid drying or heavy matrix processing

Safety features such as dual‐switch activation of the manifold prevent accidental closure and protect users from pinch hazards. Compliance with international equipment directives ensures suitability for regulated environments.

Benefits and Practical Applications

The PRESSURE+ 96 manifold offers:

• High‐throughput SPE processing of up to 96 samples in parallel
• Reduced sample variability through controlled pressure delivery
• Flexible workflows supporting conditioning, loading, washing, drying, and elution
• Compatibility with a broad range of SPE chemistries and plate formats
• Minimized cross‐contamination via dedicated sealing gaskets and waste trays

It is well suited for environmental residue screening, clinical sample prep, QA/QC testing, and method development.

Future Trends and Potential Applications

Emerging directions include integration with robotic liquid handlers for fully automated SPE workflows, networked pressure and flow monitoring via digital sensors, and data logging for audit trails. Miniaturization toward 384‐well formats and greener solvent consumption strategies will expand applications in high‐density screening and regulatory compliance. Cloud‐based method libraries and AI‐assisted optimization may further streamline method development and validation.

Conclusion

The Biotage PRESSURE+ 96 system provides a robust, safe, and adaptable platform for high‐throughput SPE under positive pressure. Its precise flow control, modular design, and compliance with safety standards make it a valuable tool for analytical chemists seeking reproducible and efficient sample preparation.