Biotage® PRESSURE+ 48 and PRESSURE+ 96 Positive Pressure Manifolds

Significance of the Topic

Positive pressure solid-phase extraction (SPE) manifolds support consistent, high-throughput sample preparation in analytical chemistry. They overcome common vacuum-based limitations such as channeling and inconsistent flow rates.

Objectives and Study Overview

This application note presents the design and performance of two positive pressure manifolds: PRESSURE+ 96 and PRESSURE+ 48. It aims to demonstrate uniform flow, compatibility with plate and cartridge formats, and improved reproducibility for viscous and standard samples.

Methodology and Used Instrumentation

Both systems employ an independent port design and gas regulators with rotameters to apply up to 100 psi. The PRESSURE+ 96 features an auto-adjusting upper manifold to fit all 96 and 48 well plate formats, while the PRESSURE+ 48 supports 1 to 48 SPE cartridges up to 6 mL. Stationary phase material RENSA RP is characterized by 90 μm particle size, 120 Å pore diameter, and 400 m2/g surface area.

Main Results and Discussion

Testing confirmed uniform solvent flow across all ports, even with partially loaded plates or cartridges. Viscous sample matrices were processed efficiently under elevated pressures. Component compatibility allows for interchangeable racks and collection vessels without compromising seal integrity.

Benefits and Practical Applications

• Consistent recovery and reproducibility in SPE, protein precipitation, and supported liquid extraction
• Parallel processing of up to 96 samples for increased throughput
• Flexibility to handle a range of sample viscosities and volumes
• Simple setup and integration into existing laboratory workflows

Future Trends and Potential Applications

Advancements may include automation integration, real-time flow monitoring, and expansion to 384-well formats. Emerging applications in metabolomics, environmental analysis, and biopharma QA/QC can benefit from positive pressure SPE technologies.

Conclusion

The PRESSURE+ series offers robust, user-friendly platforms for uniform SPE processing. Their pressure-based design ensures reliable performance across diverse sample types and scales, enhancing laboratory efficiency and data quality.

Reference

• TN0027.V.1 Application Note for Biotage PRESSURE+ 48 and PRESSURE+ 96 Positive Pressure Manifolds