<h3>The recommended procedure for H53 method integration in Clarity (method 1):</h3>
<ol>
<li>Use Baseline – Lock on the whole chromatogram.
</li>
<li>Apply Baseline – Allow Crossing to the whole chromatogram with the option To Start/End.
</li>
<li>Via Peak – Add positive, add the following peaks:
</li>
</ol>
<ul>
<li>A peak with the start time just before the solvent peak (this is the initial point of the baseline) and the end time at the beginning of the first fraction.</li>
<li>The first fraction (C10) peak with the start time exactly at the end time of the previous peak and the end time at the end of the fraction.</li>
<li>In a similar manner, i.e., with non-overlapping time frames, add the other peaks for remaining fractions (optionally, C10–C20, C20–C30, and C30–C40).</li>
</ul>
<ol start="4">
<li>Set Baseline – Together and Baseline – Forward horizontal over the entire chromatogram.
</li>
<li>Assign peaks to respective fractions using Force Peak Name (exact times are filled automatically when selecting the peak from the graph).
</li>
<li>Create a calibration file for the present fractions.
</li>
</ol>
When the chromatogram is integrated appropriately, you can use the Method - Save as Template command from the Chromatogram window to save the method as a template method, so it can be used for further measurements. In the case you have already measured all the samples, you can apply the integration on all your chromatograms via Analysis – Batch reprocess by method.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_How_to_determine_non_volatile_hydrocarbons_according_to_DIN_H53_ISO_16703_1_4d7e6aabf8/DataApex-How-to-determine-non-volatile-hydrocarbons-according-to-DIN-H53-(ISO-16703)-1.jpg" alt="DataApex - How to determine non-volatile hydrocarbons according to DIN H53 (ISO 16703)? 1"/>
Note: In the case, that the amounts of some compounds in standards are not known, it is necessary to set the Curve Fit Type to Free Calibration on the tabs of these compounds in the Calibration window.
<h4>Example of an evaluated chromatogram</h4>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_How_to_determine_non_volatile_hydrocarbons_according_to_DIN_H53_ISO_16703_2_9da75db54b/DataApex-How-to-determine-non-volatile-hydrocarbons-according-to-DIN-H53-(ISO-16703)-2.jpg" alt="DataApex - How to determine non-volatile hydrocarbons according to DIN H53 (ISO 16703) - 2"/>
<h3>An alternative procedure for H53 method integration in Clarity (method 2):</h3>
<ol>
<li>Use the same Peak Width and Threshold as in standard integration.
</li>
<li>Apply Baseline – Together to the whole chromatogram.
</li>
<li>Apply Baseline – Forward Horizontal to the whole chromatogram.
</li>
<li>Mark all the peaks between C10 and C40 as a group (the group boundary lines may exceed the C10 and C40 peaks, only fully contained peaks will be included).
</li>
<li>Amend the peak starts and ends by manual peak tools if necessary for the C10 and C40 (use the Peak - Both, with the A time corresponding to the respective C10 or C40 time and B time positive or negative, respectively. The times are relative, i.e., will be shifted accordingly in other chromatograms).
</li>
<li>Create calibration file for C10, C40, and Group A peaks.
</li>
</ol>
If the integration is not correct at this point (e.g., the integrated area for Group A between C10 and C40 peaks does not start/end exactly where the C10/C40 peaks end/start respectively, as shown in the figure below), an additional step might be added after step 3.: Apply again Baseline – Together to the whole chromatogram. Continue with the remaining steps.
To subtract a baseline, in the Method Setup – Advanced dialog, set the baseline chromatogram, and in the Matching, set No Change. The baseline file should be acquired on the stabilized system and preferably without injection of solvent or at least with a lower volume of the solvent. Otherwise, negative artifacts will appear, interfering with the correct baseline setting in Clarity later on.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_How_to_determine_non_volatile_hydrocarbons_according_to_DIN_H53_ISO_16703_3_cbe418722f/DataApex-How-to-determine-non-volatile-hydrocarbons-according-to-DIN-H53-(ISO-16703)-3.jpg" alt="DataApex - How to determine non-volatile hydrocarbons according to DIN H53 (ISO 16703) - 3"/>
<blockquote>
There is a condition that must be met:
</blockquote>
<blockquote>
<ul>
<li>The drawn baseline must not cross the chromatogram line (the negative areas arising do not have any physical meaning).</li>
</ul>
</blockquote>
This approach is generally less reliable and more sensitive to the choice of Integration Algorithm. 
<h4>Example of an evaluated chromatogram</h4>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_How_to_determine_non_volatile_hydrocarbons_according_to_DIN_H53_ISO_16703_4_46736a7950/DataApex-How-to-determine-non-volatile-hydrocarbons-according-to-DIN-H53-(ISO-16703)-4.jpg" alt="DataApex - How to determine non-volatile hydrocarbons according to DIN H53 (ISO 16703) - 4"/>
<h3>Sample data for Clarity</h3>
<ul>
<li><a href="https://downloads.dataapex.com/documentation/clarity/other/DIN_H53.zip">DIN_H53.zip</a> - zipped chromatograms, methods, and calibrations.</li>
<li><a href="https://downloads.dataapex.com/documentation/clarity/other/Din-H53-processed.dgz">Din-H53-processed.dgz</a> - archived Clarity Project to restore with Clarity via the Archive/Restore function</li>
</ul>

How to determine non-volatile hydrocarbons according to DIN H53 (ISO 16703)?

<h3>Promotion Alert!</h3>
For a limited time, customers who replace their existing chromatography software or integrator with <a href="https://lcms.labrulez.com/products/999">Clarity</a> or Clarity Lite Chromatography Software will receive a 20% discount on the software! This promotion is applicable also for old DataApex software (CSW17/CSW32). The promotion is valid through August 31, 2024.
Please contact <a href="https://www.dataapex.com/">DataApex</a> or your <a href="https://lcms.labrulez.com/products/999">distributor</a> for more details.
<h4>Why migrate to Clarity/Clarity Lite?</h4>
<ul>
<li>User-friendly interface.</li>
<li>Scalable solution.</li>
<li>Comprehensive data analysis tools.</li>
<li>Wide range of over 1000 controlled instruments.</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Replace_your_current_Chromatography_Software_with_Clarity_and_Save_Big_190b40fbee.jpg" alt="DataApex: Replace your current Chromatography Software with Clarity and Save Big!"/>

Replace your current Chromatography Software with Clarity and Save Big!

<a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/1007">NGA Extension</a> is a software module for natural gas and liquefied petroleum gas data processing. You no longer need an external tool for gas properties calculations since Clarity contains the complete workflow of data acquisition, peak calibration, reporting, and gas properties calculations based on supported norms. It is possible to perform summary calculations of gas properties from multiple signals and chromatograms.<ul><li>Calculates gas properties in compliance with internationally recognized standards ISO 6976-95, ASTM D 3588-98, GPA 2172-09 for Natural Gas or ASTM D 2421-02, ASTM D 2598-02, ISO 8973-97 / EN589-04 for Liquefied Petroleum Gas.</li><li>The gas properties can be calculated across multiple signals and even across multiple chromatograms.</li><li>It is possible to include additional norms based on customers feedback.</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?manufacturerItems=58&amp;search=NGA">NGA Extension</a> is an optional addition to <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/999">Clarity software</a>, it cannot be used as a standalone program.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_NGA_Extension_a32a83147a.jpg" alt="DataApex -Clarity NGA Extension" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Clarity_NGA_Extension_a32a83147a.jpg 245w," sizes="100vw" width="1212" height="767"><h4>Features - calculated properties according to norms</h4><h5>ISO 6976-95</h5><ul><li>Compression Factor (for real gas only)</li><li>Mean Molecular Weight</li><li>Relative Density</li><li>Density</li><li>Superior Calorific Value</li><li>Inferior Calorific Value</li><li>Wobbe Index</li></ul>ISO 6976-16 / ISO 6976-95 Natural gas: Calculation of calorific values, density, relative density, and Wobbe index from composition.<h5>ISO 8973-97 / EN589-04</h5><ul><li>Absolute Vapour Pressure (for Molar and Mass Percent only)</li><li>Gauge Vapour Pressure (for Molar and Mass Percent only)</li><li>Octane Number</li></ul>ISO 8973-97 / EN 589+A1: Standards for calculation methods for density and vapor pressure in liquefied petroleum gases.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_software_NGA_Natural_Gas_Analysis_Extension_2_5effa3549d.jpg" alt="DataApex: Clarity software - NGA (Natural Gas Analysis) Extension" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Clarity_software_NGA_Natural_Gas_Analysis_Extension_2_5effa3549d.jpg 195w," sizes="100vw" width="1005" height="806"><h5>ASTM D 3588-98 and GPA 2172-09</h5><ul><li>Molar Mass</li><li>Molar Mass Ratio</li><li>Compressibility Factor</li><li>Relative Density</li><li>Ideal Heating Value (also in BTU - British Thermal Units)</li><li>Real Heating Value (also in BTU - British Thermal Units)</li></ul>ASTM D 3588-98: The standard practice for calculating heating values, compressibility factor, and relative density of gaseous fuels.GPA 2172-09: Calculation of gross/net heating values, relative density, and compressibility factor for natural gas mixtures from compositional analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_software_NGA_Natural_Gas_Analysis_Extension_93e81ffce8.jpg" alt="DataApex: Clarity software - NGA (Natural Gas Analysis) Extension" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Clarity_software_NGA_Natural_Gas_Analysis_Extension_93e81ffce8.jpg 176w," sizes="100vw" width="1023" height="905"><h5>ASTM ASTM D 2421-02 and D 2598-02</h5><ul><li>Vapor Pressure</li><li>Relative Density</li><li>Motor Octane Number</li></ul>ASTM D 2421-02: The standard practice for interconversion of analysis of C5 and lighter hydrocarbons to gas-volume, liquid-volume, or weight basis.ASTM D 2598-02: The standard practice for calculation of certain physical properties of liquefied petroleum (LP) gases from compositional analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_software_NGA_Natural_Gas_Analysis_Extension_3_6e44e2d279.jpg" alt="DataApex: Clarity software - NGA (Natural Gas Analysis) Extension" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Clarity_software_NGA_Natural_Gas_Analysis_Extension_3_6e44e2d279.jpg 176w," sizes="100vw" width="853" height="757"><h4>Specification</h4><ul><li>Part No.: A32</li><li>Related products: Clarity (p/n C50)<ul><li>Clarity Offline (p/n C59) GC and AS Control module (p/n A23 and A26)</li></ul></li></ul>

Clarity software - NGA (Natural Gas Analysis) Extension

For analytical scientists working with chromatography and mass spectrometry, protecting sensitive information is crucial. Advances in instrument and software technology, combined with increased demands for in-depth analytics, has significantly increased the volume of valuable data that must be safeguarded from unauthorized access and potential breaches. One effective strategy to enhance security is to centralize data management, storing data away from the initial point of acquisition. Let us explore how this approach can significantly strengthen security for chromatography and mass spectrometry users and facilitate adherence to regulatory compliance measures surrounding electronic records.&nbsp;<h4>Understanding the analytical science landscape&nbsp;</h4>Chromatography and mass spectrometry are critical techniques in many fields including pharmaceuticals, environmental analysis and biochemistry. The data generated from these methods includes intricate details about potentially proprietary chemical compositions and biological information. Protecting this data is crucial to maintaining the integrity of scientific research and the competitive edge of organizations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Enhanced_Security_Through_Centralized_Data_Management_A_guide_for_Chromatography_and_Mass_Spectrometry_Users_95f6d464f1.webp" alt="Thermo Fisher Scientific: Enhanced Security Through Centralized Data Management - A guide for Chromatography and Mass Spectrometry Users" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Enhanced_Security_Through_Centralized_Data_Management_A_guide_for_Chromatography_and_Mass_Spectrometry_Users_95f6d464f1.webp 245w," sizes="100vw" width="1200" height="600"><h4>The data security challenge&nbsp;</h4>Data acquisition, processing, storage and reporting has traditionally been conducted on isolated, static hardware-bound workstations. This approach presents significant security risks:<ul><li>Single point of failure: An isolated system with poor backup strategies can be vulnerable to catastrophic data loss via accidental damage or environmental changes. Any compromise can lead to data corruption, loss or unauthorized alterations.&nbsp;</li><li>Accessibility and convenience: Storing and processing data in a noisy lab environment can be inconvenient and impractical. Technical staff are often competing for access to instruments using the same workstation that others need for processing. This often leads to users manually transferring data outside of a controlled system for processing elsewhere. &nbsp;</li></ul><h4>The case for centralized data management&nbsp;</h4>Centralizing data management involves storing data in a central server location, away from the initial point of data acquisition. Here is how this approach can enhance security and usability for chromatography and mass spectrometry users:<ul><li>Reduced risk: A centralized system that has redundancies and backups in place can alleviate the risks associated with a single point of failure. Attackers trying to gain access will also have to deal with robust security measures not typically seen with isolated workstations&nbsp;</li><li>Enhanced data integrity: With separate systems for acquisition and storage, the integrity of the data is better preserved. Any unauthorized changes on the acquisition workstation will not directly affect the stored data.&nbsp;</li><li>Remote access and flexibility: Centralized data storage allows users to securely and conveniently access data from anywhere without manual transfer or copying the data.&nbsp;</li></ul><h4>Data retention, integrity and auditing&nbsp;</h4>Compliance with regulatory bodies such as the FDA (Food and Drug Administration) and &nbsp;EMA (European Medicines Agency) involves meeting stringent requirements for data integrity, security and retention. Centralized data management can help meet these requirements.<ul><li>Data integrity and audit trails: Separating acquisition from storage lets organizations ensure long-term raw data integrity and traceability by controlling access and providing audit trails.&nbsp;</li><li>Secure data retention: Regulations often mandate that data submissions be stored securely for extended periods. Dedicated data storage solutions can be designed to meet these requirements of large data volumes in a cost-effective way, while also protecting data from unauthorized access, accidental damage and environmental threats. &nbsp;</li></ul><h4>Practical implementation considerations for data security&nbsp;</h4>Chromatography and mass spectrometry users looking to deploy a centralized data management system should consider some data security essentials:<ul><li>Secure data transfer protocols: Use of encrypted channels for transferring data from the acquisition point to the central storage system.&nbsp;</li><li>Robust authentication mechanisms: Multi-factor authentication (MFA) to control access to both acquisition and storage systems. Single sign-on (SSO) systems also offer convenience.&nbsp;</li><li>Accessible audit trails and monitoring: Conducting regular security audits and continuous monitoring to detect and respond to potential threats promptly.&nbsp;</li><li>Leverage cloud solutions: Consider using cloud-based solutions that offer built-in security features for both data acquisition and storage. Many cloud providers offer specialized services that can help segregate these functions securely.&nbsp;</li><li>Compliance-focused data management: Ensure that the data management strategy aligns with regulatory requirements for data integrity, security and retention. This includes maintaining detailed records of data acquisition, storage, and processing activities.&nbsp;</li></ul><h4>Conclusion&nbsp;</h4>Centralizing data management by storing data away from the initial point of acquisition is a powerful strategy to enhance data security and facilitate regulatory compliance. Centralized data can be securely managed and shared remotely, allowing users to access data independently from the lab-bound workstations used for acquisition. Adopting secure data transfer protocols and robust authentication mechanisms can be made easier with modern cloud solutions. Scientists should feel empowered by these tools to protect their valuable data and maintain the integrity of their research. &nbsp;

Enhanced Security Through Centralized Data Management: A guide for Chromatography and Mass Spectrometry Users

The latest version of <a target="_blank" rel="noopener noreferrer" href="https://mestrelab.com/main-product/mnova?utm_source=adv&amp;utm_medium=labrulez&amp;utm_campaign=june&amp;utm_term=mnova%2016&amp;utm_content=blogpost">Mnova</a> is now available, delivering a powerful set of tools, performance enhancements, and automation capabilities to support and accelerate your analytical workflows.Building on these advancements, Mnova 16 introduces expanded functionalities for NMR, LC/GC-MS, and electronic or vibrational spectroscopy, offering improved ways to process, analyze, and report your analytical data – ensuring greater accuracy, efficiency, and ease of use.<h4>What’s new in Mnova 16?</h4><h5>Discover the Top Features:</h5><ul><li>Enhanced Python Engine – Streamline scripting and automation directly within Mnova.</li><li>Allotrope ASM support – Seamless import/export of NMR data to increase interoperability.</li><li>New Command Palette – Access key functions faster with an intuitive, searchable command bar.</li><li>And more – Explore the full list of <a target="_blank" rel="noopener noreferrer" href="https://mestrelab.com/resources-by-product/resources-mnova/top-features-in-mnova-16.html?utm_source=adv&amp;utm_medium=labrulez&amp;utm_campaign=june&amp;utm_term=mnova%2016&amp;utm_content=blogpost">Top Features.</a></li></ul>Mnova 16 also introduces significant updates across plugins and bricks like Mnova NMR, Mgears, Mnova Hub, Chrom Cal, qNMR, and more.<blockquote>Check the full details in the <a target="_blank" rel="noopener noreferrer" href="https://mestrelab.com/changelog/whats-new-in-mnova-16.html?utm_source=adv&amp;utm_medium=labrulez&amp;utm_campaign=june&amp;utm_term=mnova%2016&amp;utm_content=blogpost">Changelog</a>.</blockquote><h5>Ready to upgrade?</h5><ul><li>Don’t miss out on the latest innovations – visit our website to download <a target="_blank" rel="noopener noreferrer" href="https://mestrelab.com/download?utm_source=adv&amp;utm_medium=labrulez&amp;utm_campaign=june&amp;utm_term=mnova%2016&amp;utm_content=blogpost">Mnova 16</a> and take your workflows to the next level.</li></ul><h5>Not a user yet?&nbsp;</h5><ul><li>Try <a target="_blank" rel="noopener noreferrer" href="https://mestrelab.com/evaluate/single?utm_source=adv&amp;utm_medium=labrulez&amp;utm_campaign=june&amp;utm_term=mnova%2016&amp;utm_content=blogpost">Mnova 16 free for 45 days</a> and experience the full potential of our latest release.</li></ul>

Mnova 16 is Live!

Clarity 10.1 expands calibration capabilities to help you work more confidently and efficiently. From clearer guidance and better warnings to flexible internal standard evaluation, this update streamlines calibration development, improves traceability, and supports compliance where it matters most.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_10_1_397aba9bab.jpg" alt="DataApex: Clarity 10.1 released" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Clarity_10_1_397aba9bab.jpg 143w," sizes="100vw" width="849" height="925"><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/P063_Clarity_10_1_New_Features_283323fcd2.pdf">Clarity 10.1 highlights</a></h4><ul><li>ISTD from a different signal</li><li>Determination Coefficient (R2)</li><li>Enhanced calibration warnings and blank handling</li><li>Electronic Signature improvements</li><li>New and updated control modules</li></ul><h5>Internal standard from another signal</h5>Clarity 10.1 introduces support for evaluating results using an internal standard from a different signal within the same chromatogram. This is especially useful for workflows like ICP-MS, where some signals may lack an ISTD peak.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Internal_standard_from_another_signal_2f2ac068f8.jpg" alt="DataApex: Internal standard from another signal" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Internal_standard_from_another_signal_2f2ac068f8.jpg 228w," sizes="100vw" width="380" height="260"><h5>Determination Coefficient (R²)</h5>Clarity now displays the Determination Coefficient (R²) alongside the Correlation Coefficient in each compound's calibration curve tab.Determination Coefficient (R²) gives you a more accurate view of how well your calibration model fits the data - especially valuable during method development and validation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Determination_Coefficient_57673d0ac3.jpg" alt="DataApex: Determination Coefficient" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Determination_Coefficient_57673d0ac3.jpg 228w," sizes="100vw" width="380" height="260"><h5>Enhanced calibration warnings and blank handling</h5>Clarity 10.1 helps you catch issues earlier and set up calibration with greater confidence. Warnings are now more visible and more specific - appearing above both the Results Table and the Calibration Summary Table. When multiple issues occur, an ellipsis appears with full details available on hover.Blank handling has also been clarified: blanks are included only when Ignore Origin is selected. If the origin is used, blanks are excluded, and Clarity now clearly warns and visually shows when a blank is ignored.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Dataapex_Enhanced_calibration_warnings_and_blank_handling_e9fe7fa31e.jpg" alt="Dataapex: Enhanced calibration warnings and blank handling" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Dataapex_Enhanced_calibration_warnings_and_blank_handling_e9fe7fa31e.jpg 228w," sizes="100vw" width="380" height="260"><h5>Signature improvements</h5>Clarity 10.1 improves how signatures are handled, making it easier to understand what is and isn't signed. Chromatograms opened with a linked calibration are always marked Not Signed. Once signed, the mode switches to Stored Calibration, so you always see the final, signed result. Even historical versions now reliably show their signature state and details.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Dataapex_Signature_improvements_a2bebe7875.jpg" alt="Dataapex: Signature improvements" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Dataapex_Signature_improvements_a2bebe7875.jpg 228w," sizes="100vw" width="380" height="260"><blockquote>Explore the complete list of all changes, fixes, and additions in version 10.1.<a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1">See the complete changelog</a></blockquote>

Clarity Chromatography Software 10.1 released!

Clarity's Dual Chromatogram Mode is a specialized configuration designed for analytical systems that require simultaneous acquisition and processing of data from multiple detectors. Most commonly applied in dual tower gas chromatograph (GC) systems, this mode allows for the real-time acquisition of two chromatograms - each representing distinct detector outputs.This guide provides an in-depth look into the configuration, operational mechanics, and benefits of using Dual Chromatogram Mode within <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/999">Clarity software</a> by DataApex.<h4>Tools and System Requirements</h4><h5>Prerequisites</h5><ul><li>Clarity Chromatography Software</li><li>Dual Detector System (e.g., Dual Tower GC)</li><li>Properly configured System Configuration files</li><li>Signal Channels connected to respective detectors</li></ul><h4>System Configuration and Activation</h4><h5>Enabling Dual Chromatogram Mode</h5>To enable this mode, the user must access the System Configuration settings and individually activate Dual Chromatogram Mode for each instrument. This configuration determines how signal inputs from multiple detectors are routed.<h5>Assigning Signals</h5>Once activated:<ul><li>Signals are split and assigned to either the Front or Back chromatogram.</li><li>This signal labeling is consistent throughout the Clarity software workflow, ensuring accurate data mapping and separation.</li></ul><h4>Method Setup and Calibration</h4><h5>Method Adaptation</h5>Each method needs to be adapted to work with Dual Chromatogram Mode:<ul><li>Signals are distinctly labeled by position (Front/Back).</li><li>Each method tab includes separate configuration areas for each signal path.</li></ul><h5>Calibration Files</h5><ul><li>If calibration is used, individual calibration files must be assigned to the front and back chromatogram paths within the method settings.</li></ul><h4>Interface and Workflow Adjustments</h4><h5>Chromatogram and Instrument Windows</h5><ul><li>The Instrument Window is visually adapted to display both signal positions side by side.</li><li>The Chromatogram Window clearly labels which detector the chromatogram originated from by indicating position in the Measurement Conditions section.</li></ul><h5>Sequence Window Enhancements</h5><ul><li>Identification columns in the Sequence Window are designated separately for each chromatogram.</li><li>Other columns (like sample names, batch parameters) are shared and applied to both positions.</li></ul><h4>Reporting and Data Processing</h4><h5>Report Customization</h5><ul><li>Generated reports automatically include a dual mode label, indicating the chromatogram's origin (Front/Back).</li><li>All standard report formatting options remain supported.</li></ul><h5>Data Evaluation</h5><ul><li>The mode supports independent processing for each chromatogram.</li><li>All standard reprocessing operations—such as integration, calibration application, and result analysis - are supported independently for each position.</li></ul><h4>Conclusion</h4>Clarity’s Dual Chromatogram Mode offers robust functionality for dual-detector systems, providing synchronized acquisition while maintaining the independence of signal processing. This ensures analytical accuracy, ease of comparison, and flexible evaluation, especially in multi-detector chromatographic setups.<blockquote>For more information, see the Dual Chromatogram Mode manual:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/paper/30514">Clarity Extension DUAL CHROMATOGRAM MODE</a></li></ul>For further learning, watch more instructional content on the:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@DataapexPrague">DataApex YouTube channel</a></li></ul></blockquote>

Dual Chromatogram Mode in Clarity: A Technical Overview

<h4>1. Introduction</h4>This article provides a comprehensive and structured troubleshooting guide for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity chromatography software </a>by DataApex. Based on the instructional video titled “What to do when you have issues with Clarity”, it outlines common errors, diagnostic methods, and recommended procedures for addressing technical problems in both software and hardware configurations.<h4>2. Required Tools and Environment</h4>To effectively troubleshoot Clarity, ensure you have access to the following:<ul><li>Clarity Software (version 9.x or 10.x)</li><li>Hardware key (HW key) – for license authorization</li><li>Windows administrator privileges</li><li>Connected instrumentation (GC/LC systems, A/D converters)</li><li>Diagnostic tools:<ul><li>Windows Event Viewer</li><li>Clarity Device Configuration Manager</li></ul></li></ul><h4>3. Common Errors and Solutions</h4><h5>3.1 File Access Errors</h5>Symptom:<ul><li>Error messages such as: "Unknown error accessing ... Temp\Clarity\$ch1run.raw"</li></ul>Cause:<ul><li>Lack of write permissions to the Temp directory.</li></ul>Solution:<ol><li>Ensure the Windows user has full access to the Temp\Clarity folder.</li><li>Run Clarity as Administrator to bypass restricted permissions.</li></ol><h5>3.2 Hardware Key Not Recognized</h5>Symptoms:<ul><li>"Clarity is unable to find HW key"</li><li>"Missing keylock drivers"</li><li>"Blocked HW key"</li></ul>Causes:<ul><li>Key not plugged in, damaged USB port, or missing driver.</li></ul>Solution:<ol><li>Reinsert the HW key into a direct USB port (avoid USB hubs).</li><li>If drivers are missing or outdated, reinstall the HW key drivers and restart the PC.</li><li>Contact DataApex support if the key is blocked or invalid.</li></ol><h5>3.3 Visual C++ Redistributable Installation Fails</h5>Error:<ul><li>"Exit code: 1618" during Clarity installation.</li></ul>Cause:<ul><li>Another installation is already running (e.g., Windows Update).</li></ul>Solution:<ul><li>Wait for the current installation to finish, restart the system, and rerun the Clarity installer.</li></ul><h5>3.4 Instrument Communication Failure</h5>Error:<ul><li>"Cannot establish communication with XXX SN xxx!"</li></ul>Possible Causes:<ul><li>Instrument is turned off or disconnected.</li><li>Wrong or missing driver.</li><li>Incorrect COM port or IP address.</li></ul>Solution:<ol><li>Power on the instrument and verify all connections.</li><li>Use Clarity → System Configuration → Devices to verify driver and port settings.</li><li>Restart the driver or reassign the port if needed.</li></ol><h5>3.5 RAW File Creation Errors</h5>Error:<ul><li>"Cannot create RAW file"</li><li>"Cannot create $ch1temp.raw"</li></ul>Cause:<ul><li>Clarity fails to rename the RAW file due to a file conflict or improper termination.</li></ul>Solution:<ul><li>Ensure the previous acquisition was closed properly.</li><li>Clear error dialogs and restart the acquisition.</li></ul><h5>3.6 Project Change Requires Restart</h5>Error:<ul><li>"Changing project requires to restart instrument."</li></ul>Solution:<ul><li>Save your changes and allow the instrument to restart as prompted.</li></ul><h5>3.7 Configuration File Corruption or Version Conflict</h5>Errors:<ul><li>"Configuration file is not valid"</li><li>"Incompatible device driver"</li></ul>Cause:<ul><li>Attempting to load a project or method created in a newer software version.</li></ul>Solution:<ol><li>Use backup files provided by Clarity.</li><li>Reconfigure devices manually if needed.</li><li>Install matching drivers compatible with your current Clarity version.</li></ol><h5>3.8 Method and Table Errors</h5>Common Issues:<ul><li>"Fraction table contains overlapping time intervals"</li><li>"Too many peaks in chromatogram"</li><li>"Method file does not match instrument configuration"</li></ul>Solution:<ul><li>Adjust fraction time intervals to prevent overlaps.</li><li>Tweak peak detection settings (width, threshold).</li><li>Modify or recreate the method to reflect hardware changes.</li></ul><h4>4. General Troubleshooting Workflow</h4><ol><li>Reboot your PC – resolves many temporary issues.</li><li>Check file and folder permissions – especially the Temp and project directories.</li><li>Verify hardware connections – including USB and serial ports.</li><li>Update or reinstall drivers – HW key, devices, or Clarity modules.</li><li>Restore from backups – when configuration or method files are corrupted.</li></ol><h4>5. Analysis and Best Practices</h4><ul><li>70% of reported issues are related to permissions, HW key problems, or outdated drivers.</li><li>Always keep Clarity and related drivers updated from the official source.</li><li>Ensure project and method files are compatible with the Clarity version you are using.</li><li>Use administrative privileges when installing or configuring hardware/software.</li></ul><h4>6. Conclusion</h4>Clarity is a robust chromatography software platform, but like any system, it may encounter operational or configuration errors. This guide outlines practical and verified troubleshooting techniques derived from real-world scenarios shown in the DataApex tutorial video.For persistent or unclear issues, consult the DataApex Support Portal for documentation, driver downloads, and direct contact with technical support.<blockquote><h5>Quick Tips Recap</h5><ul><li>Always start with a reboot and check permissions.</li><li>Reinstall drivers if devices or keys are not recognized.</li><li>Use correct project/method versions matching your Clarity installation.</li></ul></blockquote>

Troubleshooting Guide: Resolving Issues with Clarity Chromatography Software

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