<p><strong>The biennial conference is organized by Department of Analytical Chemistry, Faculty of Science, Palacky University in cooperation with Czech Chemical Society and Czech Society for Mass Spectrometry. It is organized as a meeting of the scientists from the universities, academic institutes and experts from the routine analytical laboratories.</strong></p>
<p>The scientific program will deal with all areas of separation science from fundamental to applied reseach in chemistry, physics, biology, medicine, forensic science and related disciplines. Official languages of the conference are English, Czech and Slovak.</p>
<h3><strong>Registration</strong></h3>
<blockquote>
<p><a href="https://chiranal2024.upol.cz/index.php?link=registration"><strong>Registration</strong></a></p>
</blockquote>
<p>Registration fee is 5000,- CZK (200 EUR) + tax 21 %.</p>
<p>Registration covers printed Program and Book of Abstracts, access to all scientific and social events held in the framework of the conference and meals.</p>
<p>The invoice should be paid until <strong>May 26th, 2024</strong>.</p>
<h3><strong>Programme</strong></h3>
<h3><strong>Monday 17th June</strong></h3>
<h5><strong>14:00 – 17:30 Registration</strong></h5>
<ul>
<li>Fort Science (foyer, ground floor), individual arrival of participants and accommodation</li>
</ul>
<h5><strong>16:00 – 16:15 Opening of the conference (Fort Science, Laudon Hall, ground floor), presence of official representatives of the conference partners</strong></h5>
<ul>
<li>Foreword: Petr Bednář</li>
</ul>
<h5><strong>16:15 – 17:15 Plenary Lecture</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<h5><strong>Toward unrivaled chromatographic resolving power: Design and development of comprehensive spatial three-dimensional liquid-phase separation technology</strong></h5>
<ul>
<li>Sebastiaan Eeltink</li>
</ul>
<h5><strong>17:30 – 17:45 Ceremonial launch of the book of Lucie Nováková, Michal Douša, Petr Česla and Jiří Urban: Modern HPLC separations in theory and practice</strong></h5>
<h5><strong>17:45 – ?? Welcome party + banquet</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UPOL_Chiranal_cover_image_6d8ebc4308/UPOL-Chiranal-cover-image.jpg" alt="Chiranal: Advances in Chromatography and Electrophoresis &amp; Chiranal 2024"/></p>
<h3><strong>Tuesday 18th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
<li>installation of posters (Faculty of Science, seminary rooms, 2nd floor)</li>
</ul>
<h4><strong>09:00 – 11:20 Lectures I: Electromigration techniques, ion exchange chromatography</strong></h4>
<ul>
<li>chairmen: Michael Lämmerhofer, Petr Bednář</li>
<li>Faculty of Science, assembly hall, 2nd floor)</li>
</ul>
<h5><strong>09:00 – 09:30 Electrokinetic methodologies for diagnostics development</strong></h5>
<ul>
<li>Anne Varenne</li>
</ul>
<h5><strong>09:30 – 10:00 Advances in microchip electrophoresis for the analysis of environmental and biological samples</strong></h5>
<ul>
<li>Marián Masár</li>
</ul>
<h5><strong>10:00 – 10:20 Determination of effective charge and ionic mobilities of highly sulfated cyclodextrins by capillary isotachophoresis and zone electrophoresis</strong></h5>
<ul>
<li>Václav Kašička</li>
</ul>
<h5><strong>10:20 – 10:40 Capillary Zone Electrophoresis – Mass spectrometry for intact protein analysis in biological fluids – possibilities and limitations</strong></h5>
<ul>
<li>Katarína Maráková</li>
</ul>
<h5><strong>10:40 – 11:00 Microcontrollers and their application in constructing analytical instrumentation</strong></h5>
<ul>
<li>Petr Kubáň</li>
</ul>
<h5><strong>11:00 – 11:20 Adsorbable organic fluorine (AOF) – a sum parameter for non-targeted screening of per- and polyfluorinated alkyl substances (PFASs) in waters</strong></h5>
<ul>
<li>Jan Soukup (<a href="https://lcms.cz/companies/176"><strong>Metrohm</strong></a>)</li>
</ul>
<h5><strong>11:20 – 11:40 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h4><strong>11:40 – 13:40 Lectures II: Approaches to method development in LC and MS, capillary chromatography</strong></h4>
<ul>
<li>chairmen: Anne Varenne, Petr Barták</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>11:40 – 12:10 How to successfully analyse oligonucleotides with liquid chromatography</strong></h5>
<ul>
<li>Davy Guillarme</li>
</ul>
<h5><strong>12:10 – 12:40 Recent applications of nano-liquid chromatography for the characterization of plant and food products</strong></h5>
<ul>
<li>Zeineb Aturki</li>
</ul>
<h5><strong>12:40 – 13:00 Personal insights on the design of experiments</strong></h5>
<ul>
<li>Jiří Urban</li>
</ul>
<h5><strong>13:00 – 13:20 How to make chromatography more sustainable</strong></h5>
<ul>
<li>Egidijus Machtejevas (Merck)</li>
</ul>
<h5><strong>13:20 – 13:40 Automated workflow for derivatized analyte analysis in LC-TIMS-MS/MS 4D-metabolomics data: Incorporating in-silico</strong></h5>
<ul>
<li>Aiko Barsch (Bruker Daltonics)</li>
</ul>
<h5><strong>13:40 – 14:40 Lunch</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>14:40 – 16:00 Poster session I (odd poster numbers) and coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h4><strong>16:00 – 18:00 Lectures III: Current trends in chromatography</strong></h4>
<ul>
<li>chairmen: Václav Kašička, Ondřej Kurka</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>16:00 – 16:30 Isomer separations in metabolomics and lipidomics</strong></h5>
<ul>
<li>Michael Lämmerhofer</li>
</ul>
<h5><strong>16:30 – 17:00 New Trends in the Lipidomic and Metabolomic Analysis</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Michal+Hol%C4%8Dapek"><strong>Michal Holčapek</strong></a></li>
</ul>
<h5><strong>17:00 – 17:20 Unveiling the Power of Deep UV LAESI/LAESCI Dual Ambient Ionization: Towards Comprehensive Molecular MSI</strong></h5>
<ul>
<li>Barbora Papoušková</li>
</ul>
<h5><strong>17:20 – 17:40 Advanced small molecule mass spectrometry</strong></h5>
<ul>
<li>Luka Milivojevic (<a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a>/Thermo Fisher Scientific)</li>
</ul>
<h5><strong>17:40 – 18:00 Employing ballistic gradients, vacuum jacketed columns and prototype benchtop multi reflecting time-of-flight (MRT) to increase lipidomic analytical throughput whilst maintainig highly confident identifications</strong></h5>
<ul>
<li>Alex Muck (<a href="https://lcms.cz/companies/50"><strong>Waters</strong></a>)</li>
</ul>
<h5><strong>18:00 – 19:00 Dinner</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>19:15 – 21:00 Excursion to the Eureka University Brewery and Department of Analytical Chemistry</strong></h5>
<h3><strong>Wednesday 19th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h4><strong>09:00 – 11:20 Lectures IV: Advances in biomolecules analysis, heritage science</strong></h4>
<ul>
<li>chairmen: Sebastiaan Eeltink, Lukáš Kučera</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Advances in chiral separation of carboranes – new phenyl ring isosteres for drug development</strong></h5>
<ul>
<li>Radim Kučera</li>
</ul>
<h5><strong>09:30 – 10:00 Analysis of selected drugs in whole blood collected with a volumetric absorptive microsampling device</strong></h5>
<ul>
<li>Petra Štěrbová-Kovaříková</li>
</ul>
<h5><strong>10:00 – 10:20 Translation of omics into clinics</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=David+Friedeck%C3%BD"><strong>David Friedecký</strong></a></li>
</ul>
<h5><strong>10:20 – 10:40 Py/GC-MS characterization of oil paintings to correlate museum environment and materials condition over time for SMARTMUS-e project</strong></h5>
<ul>
<li>Paola Lucero</li>
</ul>
<h5><strong>10:40 – 11:00 Recent chromatographic advances in plant hormone profiling methods</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Ond%C5%99ej+Nov%C3%A1k"><strong>Ondřej Novák</strong></a> (on behalf of <a href="https://lcms.cz/companies/37"><strong>Altium</strong></a>)</li>
</ul>
<h5><strong>11:20 – 13:00 Poster session II (even poster numbers) and coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h5><strong>13:00 – 14:00 Lunch</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>15:00 – 17:00 Social/sport event</strong></h5>
<ul>
<li>Bowling at Bowland Olomouc</li>
<li>A tour around Olomouc, visit of Regional Museum in Olomouc</li>
</ul>
<h5><strong>18:00 – ?? Social dinner</strong></h5>
<ul>
<li>Faculty of Science, foyer, 6th floor</li>
</ul>
<h3><strong>Thursday 20th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>09:00 – 11:00 Lectures V: Matrix effects, chiral separation, advances in stationary phases, supercritical fluid chromatography</strong></h5>
<ul>
<li>chairmen: Zeineb Aturki, Petr Fryčák</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Matrix effects in LC-MS: importance of correct calculation approach</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Lucie+Nov%C3%A1kov%C3%A1"><strong>Lucie Nováková</strong></a></li>
</ul>
<h5><strong>09:30 – 10:00 New trends in ion exchange-type chiral stationary phases and their chiral recognition mechanisms</strong></h5>
<ul>
<li><a href="https://lcms.cz/article/3991"><strong>Michal Kohout</strong></a></li>
</ul>
<h5><strong>10:00 – 10:20 Development of an easy-to-set-up multiple heart-cutting achiral-chiral LC-LC method for the analysis of branched-chain amino acids in food supplements</strong></h5>
<ul>
<li>Ghaid W.A. Abualzulof</li>
</ul>
<h5><strong>10:20 – 10:40 Rapid profiling of bioactive compounds using supercritical fluid chromatography coupled with tandem mass spectrometry</strong></h5>
<ul>
<li>Ivan Petřík</li>
</ul>
<h5><strong>10:40 – 11:00 Symbiosis of core-shell and fully porous particles: Implementing both for better HPLC and UHPLC results</strong></h5>
<ul>
<li>Jan Vlasák (<a href="https://lcms.cz/companies/51"><strong>Phenomenex</strong></a>)</li>
</ul>
<h5><strong>11:00 – 11:20 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>11:20 – 13:30 Lectures VI: Selectivity in chromatography and mass spectrometry and its optimization, analysis of natural products</strong></h5>
<ul>
<li>chairmen: Lucie Nováková, <a href="https://lcms.cz/articles?search=Tom%C3%A1%C5%A1+Pluh%C3%A1%C4%8Dek"><strong>Tomáš Pluháček</strong></a></li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>11:20 – 11:50 Targeted and untargeted analysis of psilocybin mushrooms using LC-MS</strong></h5>
<ul>
<li>Kevin Schug</li>
</ul>
<h5><strong>11:50 – 12:10 On the potential of two-dimensional liquid chromatography for environmental analysis</strong></h5>
<ul>
<li>Deirdre Cabooter</li>
</ul>
<h5><strong>12:10 – 12:30 Leveraging ion mobility spectrometry-mass spectrometry data using machine learning reveals provenance of indigo dyes</strong></h5>
<ul>
<li>Volodymyr Pauk</li>
</ul>
<h5><strong>12:30 – 12:50 Numerical optimization of gradient separations in RP and HILIC using non-linear retention models</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Petr+%C4%8Cesla"><strong>Petr Česla</strong></a></li>
</ul>
<h5><strong>12:50 – 13:10 Do we need separations in clinical diagnostics?</strong></h5>
<ul>
<li>Rutuja Patil</li>
</ul>
<h5><strong>13:10 – 13:30 Metabolomics analysis of Moringa oleifera leaves: correlation between in vitro effect on C2C12 myotubes cell line and geographical origin</strong></h5>
<ul>
<li>Mariateresa Maldini (Amedis)</li>
</ul>
<h5><strong>13:30 – 14:10 Closing of the conference and farewell drink (Faculty of Science, foyer, 2nd floor)</strong></h5>
<h5><strong>14:10 – 15:10 Lunch (university cafeteria)</strong></h5>
<h3><strong>Partners and Sponsors</strong></h3>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Chiranal_2024_Partners_e3fc1710b0/Chiranal-2024-Partners.jpg" alt="Chiranal 2024: Partners"/></p>
<ul>
<li><p><strong>Faculty of Science, Palacký University, Olomouc</strong></p>
</li>
<li><p><strong>Department of Analytical Chemistry, Palacký University, Olomouc</strong></p>
</li>
<li><p><strong>Czech Chemical Society</strong></p>
</li>
<li><p><strong>Chromatography &amp; Electrophores Czech Chemical Society</strong></p>
</li>
<li><p><a href="https://lcms.cz/companies/21"><strong>Czech Society for Mass Spectrometry</strong></a></p>
</li>
<li><p><strong>Fort Science</strong></p>
</li>
<li><p><strong>ARTECA</strong></p>
</li>
<li><p><strong>TEVA</strong></p>
</li>
<li><p><a href="https://lcms.labrulez.com/companies/1"><strong>LabRulezLCMS</strong></a></p>
</li>
</ul>
<h3><strong>Sponsors Chiranal 2024</strong></h3>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Chiranal_Sponsors_2024_14389caaaf.jpg" alt="Chiranal: Sponsors 2024"/></p>
<ul>
<li><a href="https://lcms.cz/companies/37"><strong>Altium International</strong></a></li>
<li><strong>Alven Laboratories</strong></li>
<li><strong>AMEDIS</strong>/<strong>SCIEX</strong></li>
<li><strong>Bruker</strong></li>
<li><a href="https://lcms.labrulez.com/companies/168"><strong>ECOM</strong></a></li>
<li><strong>Chromservis</strong></li>
<li><strong>MERCK</strong></li>
<li><a href="https://lcms.cz/companies/176"><strong>Metrohm</strong></a></li>
<li><a href="https://lcms.cz/companies/51"><strong>Phenomenex</strong></a></li>
<li><a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a>/<strong>Thermo Scientific</strong></li>
<li><a href="https://lcms.cz/companies/43"><strong>SHIMADZU</strong></a></li>
<li><strong>Villa Labeco</strong></li>
<li><a href="https://lcms.cz/companies/50"><strong>Waters</strong></a></li>
</ul>
<h3><strong>Permanent scientific committee</strong></h3>
<ul>
<li><p><strong>Daniel W. Armstrong (US)</strong></p>
</li>
<li><p><strong>Petr Barták (CZ)</strong></p>
</li>
<li><p><strong>Petr Bednář (CZ)</strong></p>
</li>
<li><p><strong>Salvatore Fanali (IT)</strong></p>
</li>
<li><p><strong>Bohuslav Gaš (CZ)</strong></p>
</li>
<li><p><strong>Václav Kašička (CZ)</strong></p>
</li>
<li><p><a href="https://lcms.cz/articles?search=Karel+Lemr"><strong>Karel Lemr (CZ)</strong></a></p>
</li>
<li><p><strong>Wolfgang Lindner (AT)</strong></p>
</li>
<li><p><strong>Eva Smolková (CZ)</strong></p>
</li>
<li><p><strong>Zdeněk Stránský (CZ)</strong></p>
</li>
<li><p><strong>Juraj Ševčík (CZ)</strong></p>
</li>
<li><p><a href="https://lcms.cz/articles?search=Franti%C5%A1ek+%C5%A0vec"><strong>František Švec (CZ)</strong></a></p>
</li>
<li><p><strong>Eva Tesařová (CZ)</strong></p>
</li>
</ul>
<h5><strong>Contacts</strong></h5>
<ul>
<li><p><strong>Dr. Ondřej Kurka</strong> (phone: +420 585 634 450, email: ondrej.kurka@upol.cz)</p>
</li>
<li><p><strong>Assoc. Prof. Petr Bednář</strong> (phone: +420 585 634 403, email: petr.bednar@upol.cz)</p>
</li>
</ul>


Advances in Chromatography and Electrophoresis & Chiranal 2024 (Programme)

Profiling of free fatty acids in wort using an isolator column-assisted LC-MS/MS approach

Quantification Challenges in Polymer Analysis in Urban Runoff and Wastewater using Pressurized Liquid Extraction and Double-Shot Pyrolysis-Gas Chromatography-Mass Spectrometry

<p><strong>From September until the end of 2025, the global GC/MS community can look forward to a diverse program of conferences, scientific schools, and training courses. These events are a great opportunity to discover new trends, learn advanced methods, and network with peers from around the world. Whether you are interested in fundamental research, applied solutions, or software training, there is plenty to choose from in the upcoming months.</strong></p><h5><strong>Shimadzu at 10th Lipidomics Forum</strong></h5><ul><li>14–17 September 2025 | Vienna, Austria</li><li><strong>Organizer:</strong> Shimadzu Corporation</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/907">More information and registration at this link</a></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Shimadzu_at_10th_Lipidomics_Forum_aae43f6640.jpg" alt="Shimadzu: Shimadzu at 10th Lipidomics Forum" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Shimadzu_at_10th_Lipidomics_Forum_aae43f6640.jpg 245w," sizes="100vw" width="1390" height="390"></p><h5><strong>10th Lipidomics Forum</strong></h5><ul><li>14–17 September 2025 | Vienna, Austria</li><li><strong>Organizer: </strong>University of Vienna</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/896">More information and registration at this link</a></li></ul><h5><strong>14th International Conference on Instrumental Methods of Analysis (IMA-2025)</strong></h5><ul><li>14–17 September 2025 | Kefalonia, Greece</li><li><strong>Organizer:</strong> National Technical University of Athens</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/795">More information and registration at this link</a></li></ul><h5><strong>53rd Conference on Synthesis and Analysis of Drugs 2025</strong></h5><ul><li>17–19 September 2025 | Kurdějov, Czech Republic</li><li><strong>Organizer: </strong>Masaryk University</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/678">More information and registration at this link</a></li></ul><h5><strong>21th ISC ‘Modern Analytical Chemistry’</strong></h5><ul><li>18–19 September 2025 | Prague, Czech Republic</li><li><strong>Organizer: </strong>European Chemical Society (EuChemS)</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/839">More information and registration at this link</a></li></ul><h5><strong>MSACL 2025 – 15th Annual Conference &amp; Exhibits</strong></h5><ul><li>21–26 September 2025 | Montréal, Canada</li><li><strong>Organizer:</strong> MSACL</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/731">More information and registration at this link</a></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/MSACL_2025_cover_image_762faaf59f.jpg" alt="MSACL: MSACL 2025" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_MSACL_2025_cover_image_762faaf59f.jpg 245w," sizes="100vw" width="1920" height="1080"></p><h5><strong>7th International Mass Spectrometry School (IMSS) 2025</strong></h5><ul><li>21–26 September 2025 | Vorau, Styria, Austria</li><li><strong>Organizer:</strong> International Mass Spectrometry Foundation</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/906">More information and registration at this link</a></li></ul><h5><strong>Mass Spectrometry Symposium 2025</strong></h5><ul><li>23–26 September 2025 | Costa Mesa, CA, USA</li><li><strong>Organizer: </strong>Local MS societies (CASSS)</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/897">More information and registration at this link</a></li></ul><h5><strong>ISSS 2025 – 29th International Symposium on Separation Sciences</strong></h5><ul><li>25–27 September 2025 | Belgrade, Serbia</li><li><strong>Organizer:</strong> International Symposium on Separation Sciences (ISSS)</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/730">More information and registration at this link</a></li></ul><h5><strong>GDCh Science Forum Chemistry 2025</strong></h5><ul><li>29 September – 1 October 2025 | Karlsruhe, Germany</li><li><strong>Organizer:</strong> Gesellschaft Deutscher Chemiker (GDCh)</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/755">More information and registration at this link</a></li></ul><h5><strong>International Symposium on Preparative and Process Chromatography (PREP) 2025</strong></h5><ul><li>29 September – 2 October 2025 | Philadelphia, PA, USA</li><li><strong>Organizer: </strong>LD Organisation srl</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/899">More information and registration at this link</a></li></ul><h5><strong>21st Raman Imaging Symposium</strong></h5><ul><li>29 September – 1 October 2025 | Ulm, Germany</li><li><strong>Organizer: </strong>Oxford Instruments</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/898">More information and registration at this link</a></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/21st_Raman_Imaging_Symposium_13c3033654.jpg" alt="Oxford: 21st Raman Imaging Symposium" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_21st_Raman_Imaging_Symposium_13c3033654.jpg 245w," sizes="100vw" width="1200" height="627"></p><h5><strong>SciX 2025</strong></h5><ul><li>5–10 October 2025 | Covington, KY, USA</li><li><strong>Organizer</strong>: The Federation of Analytical Chemistry and Spectroscopy Societies (FACSS)</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/796">More information and registration at this link</a></li></ul><h5><strong>ASMS – 39th Asilomar Conference on Mass Spectrometry</strong></h5><ul><li>10–15 October 2025 | Monterey, CA, USA</li><li><strong>Organizer: </strong>ASMS</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/747">More information and 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2025</strong></h5><ul><li>17–19 November 2025 | Plainsboro, NJ, USA</li><li><strong>Organizer: </strong>Eastern Analytical Symposium and Exposition</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/902">More information and registration at this link</a></li></ul><h5><strong>Invited Lecture – Jana Roithová</strong></h5><ul><li>18 November 2025 | IOCB Prague</li><li><strong>Organizer: </strong>IOCB Prague</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/709">More information and registration at this link</a></li></ul><h5><strong>TIAFT 2025</strong></h5><ul><li>23–27 November 2025 | Auckland, New Zealand</li><li><strong>Organizer: </strong>The International Association of Forensic Toxicologists</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/768">More information and registration at this link</a></li></ul><h5><strong>The Gas Chromatographers’ Training 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Global GCMS Conferences and Training 2025 – LabRulez

<h3><strong>A Deep Dive into Waters Oasis, Agilent Bond Elut, and Phenomenex Strata Workflows</strong></h3><p><a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/what-is-solid-phase-extraction-spe"><strong>Solid phase extraction (SPE)</strong></a> has become a widely used sample preparation technique across pharmaceutical, environmental, and food safety laboratories worldwide. SPE is used to separate and isolate analytes of interest from a matrix, utilizing the analytes' differential affinities between a solid stationary phase and a liquid mobile phase. The process involves four key steps: conditioning the solid phase; loading the sample onto the solid phase; washing the sample to remove interfering compounds; and eluting the analytes of interest. Following this, the now purified and concentrated sample will usually undergo further analysis such as GC-MS, LC-MS, or HPLC [1]. An often overlooked element within this method of sample preparation is the <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/article/5392"><strong>nitrogen blowdown evaporation</strong></a> that bridges SPE extraction and instrumental analysis. This discussion explores the critical role of nitrogen blowdown in enhancing the performance of three widely used SPE platforms: Waters Oasis, Agilent Bond Elut, and Phenomenex Strata.</p><h3><strong>Why Nitrogen Blowdown Matters in SPE Workflows</strong></h3><p>In the final step of SPE, elution, an organic solvent like methanol, acetonitrile, or acetone is used to remove the analytes of interest from the solid phase. This solvent must then be removed before <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/what-is-reconstitution"><strong>reconstitution</strong></a> and analysis [1]. Nitrogen blowdown provides controlled, gentle evaporation that concentrates analytes while preventing thermal degradation and sample loss that can occur with other drying methods.</p><h3><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/215"><strong>Waters Oasis SPE</strong></a><strong>: Precision Evaporation for Enhanced Sensitivity</strong></h3><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/215"><strong>Waters Oasis SPE cartridges</strong></a> and plates are renowned for their broad analyte retention capabilities, in particular for polar compounds. Their SPE products consistently deliver consistent and pure quality for sample analysis [2]. Many of their protocols consistently emphasize the importance of proper solvent evaporation following elution [3].</p><h4><strong>Critical Nitrogen Blowdown Steps in Oasis Protocols:</strong></h4><p>In Waters' documented procedures for pesticide analysis, the protocol states: "<i><strong>Blow down the acetone extract with nitrogen to ca. 0.2 ml then make to 2 ml with pure water</strong></i>" [4]. This concentration step is essential for achieving the required detection limits of pesticide compounds like diuron and its metabolites. When using the Oasis HLB cartridges to process larger sample volumes (2 L versus 200 mL), the method requires: "<i><strong>Blow down the acetone extract with nitrogen to ca. 0.8 ml then make to 2 ml with pure water</strong></i>" [4]. This increased degree of sample pre-concentration dramatically enhances the analytical sensitivity and is particularly important when analyzing trace-level environmental contaminants. The Waters protocols emphasize that this evaporation step must be performed under controlled conditions to aid in the prevention of analyte loss, making nitrogen blowdown the preferred method over thermal evaporation techniques.</p><h3><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=bond+elut"><strong>Agilent Bond Elut</strong></a><strong>: Streamlined Workflows with Controlled Evaporation</strong></h3><p>With over 40 different sorbent capabilities, Agilent's Bond Elut product line offers versatile solutions for a wide range of sample types and applications [5]. The company's method development guides consistently reference evaporation steps as critical for optimal results. Whether processing <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/environmental-sample-preparation"><strong>environmental samples</strong></a>, pharmaceutical matrices, or targeting polar and nonpolar analytes, the evaporation step remains a consistently critical factor in ensuring a method's success [5].</p><h4><strong>Nitrogen Evaporation in Bond Elut Protocol:</strong></h4><p>For Bond Elut PPL applications targeting polar compounds found within drinking water, Agilent's protocol specifies: "<i><strong>Dry the eluent under in 35 °C water bath, then reconstitute with 2 mL 10:90 MeOH/H2O</strong></i>" [6]. This controlled evaporation at moderate temperature ensures complete solvent removal while maintaining analyte integrity. The Bond Elut methodology emphasizes that the nitrogen drying step is particularly important when transitioning from organic elution solvents to aqueous-based mobile phases for <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis"><strong>LC/MS/MS analysis</strong></a> [6]. This solvent exchange is impossible without complete evaporation of the initial organic eluent.</p><h3><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=136&amp;manufacturerItems=50"><strong>Phenomenex Strata</strong></a><strong>: Advanced Polymeric Performance Enhanced by Proper Evaporation</strong></h3><p>Phenomenex Strata products, particularly their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=136&amp;manufacturerItems=50&amp;search=strata+x"><strong>Strata-X</strong></a> polymeric phases, have revolutionized SPE through water-wettable sorbents designed to work effectively with aqueous-based solutions with a simplified protocol [7]. However, even these advanced materials require proper post-extraction handling to achieve optimal results.</p><h4><strong>Strata-X Protocol Requirements:</strong></h4><p>Phenomenex documentation for their Strata-X PRO plates specifically instructs: "<i><strong>Dry: </strong></i><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/article/5411"><i><strong>Evaporate the extract to dryness</strong></i></a><i><strong> under a gentle stream of nitrogen at room temperature</strong></i>" [8]. This room-temperature evaporation is particularly important for thermally labile compounds that might degrade under increased temperatures.</p><h4><strong>Specialized Applications:</strong></h4><p>For Strata Activated Carbon applications targeting nitrosamines and other polar analytes, the protocol requires: "<i><strong>Evaporate solvent under Nitrogen to required volume and reconstitute with Methylene Chloride</strong></i>" [9]. This controlled evaporation is essential for achieving the trace detection limits required for these potential carcinogens.</p><p>The matrix removal technology within the Strata product line makes the nitrogen blowdown step even more critical. This step ensures complete removal of interfering matrix components from the eluent that could compromise downstream analysis [10].</p><h3><strong>Technical Considerations for Optimal Results</strong></h3><ul><li><strong>Temperature Control:</strong> All three manufacturers emphasize controlled temperature during nitrogen evaporation. Waters and Agilent protocols frequently specify 35°C water baths, while Phenomenex often recommends room temperature evaporation [4, 6, 8].</li><li><strong>Flow Rate Management:</strong> Gentle nitrogen flow is recommended across many manufacturers to reduce and prevent analyte loss through splashing or mechanical carryover. Excessive flow rates can lead to incomplete evaporation or sample loss.</li><li><strong>Timing Optimization:</strong> Complete solvent removal is essential, but over-drying can lead to analyte degradation or potentially irreversible adsorption to container walls. Protocols typically specify evaporation "to dryness" or to specific volumes [4, 8].</li></ul><h3><br><strong>A Nitrogen Blowdown Solution for the Leading SPE Platforms</strong></h3><p>The importance of nitrogen blowdown in an SPE workflow has been well established. To support this critical step, Organomation offers the following solutions:</p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=540&amp;search=n-evap"><strong>N-EVAP Nitrogen Evaporator</strong></a></h4><ul><li><strong>Analyte Preservation: </strong>The gentle nitrogen blowdown helps preserve heat-sensitive analytes that may degrade when exposed to high temperatures.</li><li><strong>Solvent Removal Flexibility: </strong>This system is compatible with a range of tube sizes, enabling researchers to process varying sample volumes tailored to specific laboratory needs.</li><li><strong>Controlled Evaporation: </strong>Individual gas flow control at each sample position ensures precise and consistent sample preparation, which is essential for obtaining reliable data.</li><li><strong>Temperature Control: </strong>Water bath models are available with a wide temperature range enabling optimization for use of different solvents needed for varying SPE sorbents.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Organomation_45_Position_N_EVAP_Nitrogen_Evaporator_5_20078e3969.jpg" alt="Organomation: 45 Position N-EVAP Nitrogen Evaporator" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Organomation_45_Position_N_EVAP_Nitrogen_Evaporator_5_20078e3969.jpg 156w," sizes="100vw" width="1400" height="1400"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=540&amp;search=multivap"><strong>MULTIVAP Batch Evaporator</strong></a></h4><ul><li><strong>Batch Capability:</strong> Some systems can process up to 100 samples simultaneously, making them essential for large-scale laboratory operations.</li><li><strong>Sample Standardization: </strong>Provides consistent evaporation conditions across samples, reducing sample variability which can skew interpretation.</li><li><strong>Analyte Integrity:</strong> Gentle nitrogen dry down protects delicate analytes from degradation.</li><li><strong>Efficiency: </strong>This system significantly reduces sample preparation time, allowing for faster results and turnaround time in clinical settings.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Organomation_48_Position_MULTIVAP_Nitrogen_Evaporator_2_b46408a225.jpg" alt="Organomation: 48 Position MULTIVAP Nitrogen Evaporator" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Organomation_48_Position_MULTIVAP_Nitrogen_Evaporator_2_b46408a225.jpg 156w," sizes="100vw" width="1400" height="1400"></p><h4><strong>Integration with Modern Laboratory Workflows</strong></h4><p>Modern laboratories increasingly rely on automated nitrogen evaporation systems that can process multiple samples simultaneously while also maintaining precise control over temperature, flow rate, and timing. Additionally, these systems integrate seamlessly with the 96-well plate formats offered by all three manufacturers, enabling high-throughput sample preparation. The combination of advanced SPE materials from Waters, Agilent, and Phenomenex with controlled nitrogen blowdown creates a powerful sample preparation platform that delivers the sensitivity, reproducibility, and throughput required for today's analytical challenges.</p><h4><strong>Conclusion</strong></h4><p>Nitrogen blowdown is not merely an optional step in SPE workflows, it is a critical component that enables the full potential of Waters Oasis, Agilent Bond Elut, and Phenomenex Strata products. Nitrogen blowdown ensures optimal analyte concentration, solvent exchange, and method sensitivity through gentle evaporation. Its versatility makes it applicable to diverse fields, including environmental monitoring and pharmaceutical analysis.</p><p>Laboratories investing in high-quality SPE products should equally prioritize incorporating proper nitrogen evaporation equipment and protocols to maximize their analytical capabilities and ensure consistent, reliable results. Organomation is a trusted provider of precision nitrogen evaporators, offering reliable solutions tailored to laboratory needs.</p>

The Critical Role of Nitrogen Blowdown in SPE Sample Preparation

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