Canada's Independent Certification Lab Leans on Chromatography - Mass Spectrometry

In the world of natural health product (NHP) testing and certification, analytical precision and scientific rigor are paramount. At the forefront of this field stands ISURA, Canada's premier independent, non-profit analytical testing and certification organization. With their state-of-the-art laboratory facilities and commitment to the highest testing standards, ISURA has established itself as a trusted authority in the natural health products industry.

Organomation: Canada's Independent Certification Lab Leans on Chromatography - Mass Spectrometry

ISURA: Setting the Gold Standard in Natural Health Product Certification

ISURA operates with a clear mandate: to enhance the safety of natural health products, ensure label accuracy, increase consumer confidence, and support research and development for improved test methodologies. Backed by a Scientific Advisory Committee comprised of world-renowned experts, ISURA's certification standards are among the highest globally, meeting or exceeding international standards set by organizations such as the FDA, WHO, and Health Canada [1].

What truly sets ISURA apart is their comprehensive analytical approach. Their in-house test center utilizes an impressive array of state-of-the-art equipment to perform both targeted and untargeted analyses. This dual approach allows them to verify specific active ingredients with high sensitivity while also screening for unknown contaminants or adulterants that might be present in natural health products [2].

At the core of their analytical capabilities lies an impressive suite of chromatography and mass spectrometry instruments, including:

• Headspace gas chromatography
• Gas chromatography triple-quadrupole
• Ultra-high performance liquid chromatography triple-quadrupole
• Inductively-coupled plasma mass spectrometry
• High performance liquid chromatography (HPLC) [2]

This advanced instrumentation enables ISURA to detect over 400 known pesticide and solvent contaminants down to parts per billion (ppb), confirm product authenticity, and verify that products are free from GMOs, contaminants, and adulterants [1].

The Critical Role of Sample Preparation: MICROVAP Nitrogen Evaporators

In the analytical workflow of chromatography and mass spectrometry, sample preparation represents a critical step that can significantly impact results. One common bottleneck in this process is the evaporation of organic solvents prior to analysis. This is where Organomation's MICROVAP nitrogen evaporators play a vital role.

Organomation: 24 Position MICROVAP Nitrogen Evaporator

The MICROVAP is a compact, efficient nitrogen blowdown evaporator designed specifically for laboratories working with small sample volumes. It provides an affordable solution for drying down samples in microplates or small volume tubes such as GC/autosampler vials and microcentrifuge tubes. The system works by directing a gentle stream of nitrogen gas onto the sample surface while simultaneously applying controlled heat, significantly accelerating the evaporation process.

What makes the MICROVAP particularly valuable for analytical laboratories like ISURA is its precision and versatility. The digital temperature controls and solid aluminum heating unit provide uniform heat up to 130°C with temperature accuracy of ±2°C. This precise temperature control is essential when working with heat-sensitive compounds often found in natural health products.

The MICROVAP's design features several key advantages:

1. Compact footprint, ideal for limited laboratory space
2. Digital temperature control for precise regulation
3. Customized aluminum heat blocks for optimal heat transfer
4. Even gas distribution through a specially manufactured manifold
5. Simple one-button operation to start or stop the evaporation process

For laboratories processing multiple samples, the MICROVAP offers configurations that can accommodate up to 15 samples simultaneously, making it an efficient solution for batch processing.

Nitrogen Evaporation in Action: A Critical Application in Research

Illustrative is the recent study published in Metabolites (2024, 14(5), 265) evaluating the metabolite profiles of omega-3 fatty acid fish oil formulations. In this randomized, double-blind, crossover study, researchers employed a sophisticated analytical workflow that relied on nitrogen evaporation prior to the use of analytical instrumentation. Following solid-phase extraction using a hydrophilic-lipophilic balance (HLB) sorbent, “the eluted fatty acid-rich extract was then evaporated under nitrogen and reconstituted with 50 µL of ethanol,” prior to UHPLC-MS analysis [3].

In this research the nitrogen evaporation step served multiple critical purposes:

1. It preserved the oxidation-sensitive omega-3 fatty acids (EPA and DHA) that could potentially degrade if exposed to oxygen during the concentration process.
2. It enabled effective sample concentration, which enhanced detection sensitivity for the subsequent analysis.
3. It facilitated the exchange of analytes between ethyl formate and ethanol, improving compatibility with the UHPLC-MS analysis method.

The careful sample preparation, including the nitrogen evaporation step, allowed researchers to accurately measure blood concentrations of omega-3 fatty acids and their metabolites over a 24-hour period post-administration. This ultimately revealed significant differences in the bioavailability of omega-3 fatty acids between standard softgels, enteric-coated softgels, and a novel micellar formulation.

Why Nitrogen Blowdown is Essential to Modern Analytical Chemistry

A nitrogen blowdown evaporator works by applying a steady stream of nitrogen gas just above the sample surface, which gently pushes vapor-saturated air away from the liquid, preventing it from returning to the remaining sample. This process offers several advantages over other evaporation methods:

• Preservation of Sample Integrity: The inert nitrogen environment prevents oxidation of sensitive compounds, a critical consideration when working with easily degradable substances like polyunsaturated fatty acids.
• Controlled Evaporation Rate: The combination of gentle heat and nitrogen flow allows for precise control of the evaporation process, preventing sample loss or degradation.
• Efficiency: For small volume samples in volatile or semi-volatile organic solvents, nitrogen blowdown is remarkably efficient. On average, it takes just 25 minutes to safely concentrate a 10 mL sample of methylene chloride.
• Versatility: The technique is applicable across a wide range of sample types and solvents, making it ideal for laboratories that process diverse sample matrices.

Conclusion: The Perfect Partnership

The partnership between advanced analytical instrumentation like ISURA's chromatography-mass spectrometry systems and precise sample preparation tools like Organomation's MICROVAP nitrogen evaporators represents the gold standard in natural health product testing and research.

ISURA's commitment to rigorous scientific analysis is enhanced by tools that ensure sample integrity throughout the analytical process. The MICROVAP's precise temperature control, efficient nitrogen delivery, and compact design make it an ideal solution for laboratories focused on accurate, reliable results.

As research continues to advance our understanding of natural health products and their metabolites, the role of nitrogen evaporation in preserving sample integrity will remain critical. This gentle yet effective technique ensures that what we measure truly represents what's in the sample-not artifacts of the preparation process.

For laboratories seeking to enhance their analytical capabilities, the combination of advanced chromatography-mass spectrometry and efficient nitrogen evaporation represents a powerful approach to unlocking the molecular secrets of natural health products.