Clarity chromatograhy software: Tutorial - Sequence window - Overview

The Sequence Window in Clarity Chromatography Data System (CDS) is a central tool for planning and executing analytical runs. It allows users to define and control all aspects of sample measurement, including sample identification, injection volumes, calibration settings, and post-run actions. This article provides a detailed explanation of the Sequence Window, its key components, and the functionality it offers, based on an instructional video tutorial.

Accessing the Sequence Window

The Sequence Window is accessible directly from the Instrument Window in Clarity CDS. Once opened, the main feature displayed is the Sequence Table, which contains the full overview of all analytical runs.

Structure of the Sequence Table

Status Column

• Displays the state of each row (e.g., pending, running, completed).
• All possible states with detailed descriptions are available in the Help section.

Run Column

• Determines whether a row will be measured.
• Allows the user to include or exclude specific analyses from the sequence.

Vial Columns

• Specify the sample vials to be used during the measurement process.

Sample and Sample ID Columns

• Used for sample identification and tracking.
• Essential for linking results to the correct sample.

Post-Calculation Columns

• Provide options for performing calculations after measurement.
• Useful for automated processing and reporting.

Injection Volume Column

• Directs the autosampler on how much volume should be injected per analysis.

File Name Column

• Defines the name of the resulting chromatogram file.
• Recommended: Use variables in naming to ensure unique file identifiers.

Sample Type and Level Columns

• Sample Type: Defines the role of the sample in calibration (Unknown, Standard, Blank).
• Level: Specifies the calibration level for standards.
• If Sample Type is set to Bypass, the sampler will not inject on that row.

Method Name Column

• Indicates which analytical method will be applied to the run.

Post-Run Options

• Control what happens after a run is completed.
• Additional post-run columns are hidden by default but can be enabled in the Setup Columns dialog.

Toolbar Functions

At the top of the Sequence Window, a dedicated toolbar provides essential controls:

1. File Management & Printing
   • Open, save, and print sequence files.
2. Editing Tools
   • Modify rows, copy/paste, and manage sequence table content.
3. Sequence Measurement Tools
   • Start, pause, or stop sequence runs.
   • Adjust sequence settings via the Sequence Options icon.
4. Method and Report Style Editing
   • Directly edit the method file or report style from the selected row.
5. Sequence Validity Check
   • Verifies that the sequence is properly configured before execution.

Setup Columns Dialog

• Provides control over which columns are visible in the Sequence Table.
• Useful for customizing the display according to workflow requirements.
• Includes hidden columns related mainly to post-run operations.

Best Practices

• Use descriptive sample names and IDs for traceability.
• Apply file name variables to avoid overwriting chromatograms.
• Perform a validity check before running the sequence to prevent execution errors.
• Customize the sequence layout with the Setup Columns dialog for clarity and efficiency.

Conclusion

The Sequence Window in Clarity CDS is a powerful feature for managing analytical workflows in chromatography. By providing a structured environment for defining runs, controlling injection parameters, and ensuring calibration integrity, it enhances both productivity and accuracy. Users who master the Sequence Window will benefit from streamlined operations, reliable data, and reduced risk of errors.

For further details, consult additional video tutorials on Sequence Options and related Clarity CDS functions.