How to create a sequence in Clarity software

This article provides a structured explanation of how to create and manage a measurement sequence in Clarity Chromatography Data System (CDS). It is based on the instructional video “How to Prepare a Sequence” and reformatted into a comprehensive technical article.

Introduction

In chromatography workflows, sequences are used to define a set of analytical runs in a structured, automated manner. By carefully preparing a sequence, users can save time, reduce errors, and ensure consistency across multiple injections and vials.

This guide covers:

• Creating a new sequence in Clarity
• Filling and managing sequence rows
• Configuring multiple injections and vial ranges
• Assigning sample types and calibration levels
• Using fill functions to accelerate sequence preparation
• Saving the sequence for measurement

Tools and Software

• Clarity CDS software with the sequence editor
• Access to appropriate methods and sample lists prepared in advance
• Knowledge of vial numbering and sample IDs for the autosampler

Step-by-Step Procedure

1. Creating a New Sequence

1. Open the Sequence window in Clarity.
2. Click New to create a fresh sequence table.
3. In the first row, enter the required details, such as:
   • Method name
   • Sample name
   • File name format (e.g., change variable from Sample ID to Sample)

⚠️ Tip: Values from the first row are automatically copied to new rows, ensuring consistency.

2. Defining Sequence Rows

• Check the Run box to activate the first row.
• Add as many rows as your application requires.
• In the example case:
  • A total of four rows were defined.
  • Rows can represent repeated injections from one vial or samples across multiple vials.

3. Configuring Multiple Injections and Vial Ranges

• Multiple injections from one vial:
  • Example: Row 1 → set Number of injections per vial = 2.
• Defining vial ranges:
  • Example: Row 4 → set End vial number = 7 to cover vials 4 through 7.

4. Assigning Sample Types and Calibration Levels

1. Mark the Sample type for each row.
   • Example: The first three rows are defined as Standards.
2. Assign Calibration levels:
   • Enter Level 1 in the first row.
   • Select the corresponding cells for rows 1–3.
   • Use Fill Series from the right-click menu to automatically increment levels (1, 2, 3).

5. Using Fill Down and Fill Series Functions

To speed up sequence preparation, Clarity provides convenient functions:

• Fill Down: Copies a selected cell’s value into the rows below.
• Fill Series: Automatically generates incremental values across selected cells.

Example applications:

• Fill down method names, injection volumes, or vial numbers.
• Fill series for calibration levels or sequence numbering.

6. Saving the Sequence

Once all parameters are set:

1. Click Save.
2. Assign a descriptive sequence name for future reference.
3. The sequence is now fully prepared and ready for measurement.

Discussion and Best Practices

• Consistency: Use “Fill Down” to avoid manual repetition and reduce errors.
• Calibration: Always double-check calibration levels when working with standards.
• Efficiency: Plan vial positions and injection counts before building the sequence.
• File Management: Define a logical file naming convention early to maintain clarity in data processing.

Conclusion

By following this structured approach in Clarity CDS, laboratory staff can efficiently prepare sequences for chromatography runs. The combination of sequence rows, vial configuration, and automated fill functions ensures faster setup, minimized manual errors, and improved consistency across analytical measurements.

With a properly saved sequence, the system is fully ready for automated data acquisition and subsequent analysis.