Phenomenex Strata NH2 Solid Phase Extraction (SPE)

Normal Phase Solid Phase Extraction (SPE)

This amino phase offers strong polar selectivity and hydrogen bonding under normal phase conditions or can be used as a weak anion-exchange sorbent.

Phenomenex: Strata-NH2

• Surface Area (m²/g): -
• Separation Mode: Normal Phase
• Pore Size: 100
• Particle Size: 25, 55
• Recommended Use: Strong polar selectivity and hydrogen bonding under normal phase conditions or can be used as a weak anion-exchange sorbent

Starting Method

Strata NH2 – Weak Anion-Exchange

• Condition: 1 mL Methanol
• Equilibrate: 1 mL Water, pH 6-7
• Load: Pretreated sample, pH 6-7
• Wash 1: 1 mL 25mM Ammonium Acetate Buffer, pH 6-7
• Wash 2: 1 mL Methanol, dry under vacuum for 2-5 min
• Elute Any Acid: 1 mL 5% NH4OH in Methanol
• Elute Weak Acids: 1 mL 5% Formic Acid in Methanol

Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.