Supelco Columns for USP Methods

Importance of Topic

Chromatographic methods described in the United States Pharmacopeia (USP) are critical for ensuring the purity and consistency of pharmaceutical substances. A clear mapping between USP column codes and commercial packing materials simplifies method adoption and regulatory compliance.

Objectives and Overview

This compilation aims to connect USP HPLC and GC column and support codes with Supelco product offerings. It provides an accessible reference for selecting appropriate stationary phases that meet official monograph requirements.

Methodology and Instrumentation

A detailed survey of USP codes for HPLC packings (e.g., L1, L3, L7, L8, etc.) and GC phases (G1–G13) was conducted. Each listing includes phase chemistry (alkyl, phenyl, cyano, ion‐exchange, chiral), particle or film characteristics, and recommended Supelco packings or capillary columns. Siliceous supports (S1–S12) for GC are also cataloged.

Key Results and Discussion

The summary tables reveal the most common reversed‐phase chemistries (C18, C8, phenyl‐hexyl), polar phases (HILIC, amide, cyano), ion‐exchange and chiral selectors, as well as a range of polysiloxane and polyethylene glycol GC stationary phases. Corresponding Supelco brands—including Ascentis, Discovery, SUPELCOSIL and Astec—are identified to match each USP description.

Benefits and Practical Applications

• Streamlines column selection for pharmaceutical quality control laboratories
• Ensures direct compliance with USP monograph specifications
• Facilitates method transfer, reproducibility and validation

Future Trends and Opportunities

Emerging column technologies—core-shell particles, monolithic rods and hybrid materials—promise higher efficiency and lower backpressure. Adoption of UHPLC and green solvent systems will improve throughput and sustainability. Digital selection tools and real‐time analytics integration can further optimize method development.

Conclusion

This reference guide aligns USP method requirements with Supelco’s chromatographic products, aiding laboratories in achieving regulatory compliance and robust analytical performance.

Reference

United States Pharmacopeia 37, National Formulary 32