Analysis of US EPA Method 8270D Semivolatiles Using SLB-5ms

Significance of the Topic

The accurate determination of semivolatile organic compounds in environmental matrices is essential for compliance with regulatory frameworks such as US EPA Method 8270D. Reliable quantitation of these contaminants supports risk assessment, pollution monitoring, and remediation efforts.

Objectives and Study Overview

This application report evaluates the suitability of a SLB-5ms stationary phase for the separation and detection of 72 target semivolatile analytes, eight surrogate compounds, and six internal standards as defined by US EPA Method 8270D. An optimized chromatographic method was developed and the resulting data compared to method performance criteria.

Methodology and Instrumentation

• Gas chromatography–mass spectrometry system: Agilent 6890 GC coupled to a 5973 MSD.
• Column: 30 m × 0.25 mm I.D., 0.25 μm SLB-5ms.
• Oven program: 40 °C (2 min) ramp at 22 °C/min to 240 °C then 10 °C/min to 330 °C (1 min hold).
• Injection: 0.5 μL pulsed splitless (15 psi until 0.10 min, 0.50 min total) at 250 °C.
• MS interface: 330 °C; scan range 40–450 m/z.
• Carrier gas: helium at 1.0 mL/min for 11 min, ramped to 1.5 mL/min.

Key Results and Discussion

The SLB-5ms column provided baseline resolution for the full suite of 72 analytes and surrogates with sharp peak shapes and minimal tailing. Retention times were reproducible within method specifications. Surrogate recoveries fell within 70–130%, demonstrating method accuracy. The optimized temperature program allowed elution of high-boiling analytes within a reasonable run time (<35 minutes).

Benefits and Practical Applications

• Robust separation of a diverse semivolatile compound mix in environmental samples.
• High sensitivity and reproducibility for regulatory compliance testing.
• Shorter analysis times compared to conventional columns, increasing sample throughput.

Future Trends and Applications

Advances in stationary phase chemistry and faster mass spectrometry detectors may further reduce runtime and improve detection limits. Coupling with automated sample preparation and high-resolution MS could expand applicability to emerging contaminants.

Conclusion

The SLB-5ms phase on an Agilent 6890 GC/5973 MSD delivers reliable performance for US EPA Method 8270D semivolatiles analysis, offering efficient separation, strong surrogate recovery, and compliance with regulatory requirements.

References

• Stenerson K. Analysis of US EPA Method 8270D Semivolatiles Using SLB-5ms. Application Report 391; Sigma-Aldrich Co., 2006.