SENTERRA II Compact Raman Microscop

Importance of the Topic

The evolution of compact Raman microscopy represented by SENTERRA II addresses critical demands for high sensitivity, spectral resolution, and ease of use across research and industrial laboratories.

Objectives and Overview

This summary outlines the design goals of SENTERRA II, a next-generation benchtop Raman microscope engineered to deliver research-grade spectroscopic performance with automated workflows and compliance features for quality control environments.

Methodology and Instrumentation

The core setup integrates a high-throughput dispersive Raman spectrometer, hybrid confocal optics with FlexFocus technology, multi-laser excitation (incl. optional 1064 nm), and Bruker’s SureCAL™ for permanent wavenumber calibration (<0.1 cm⁻¹ stability). The system is controlled by OPUS software, which guides users through sample visualization, spectral preview, parameter optimization, automated USP/PhEur/ASTM qualification tests, and compliance with cGMP, GLP, and 21 CFR Part 11.

Main Results and Discussion

SENTERRA II achieves 4 cm⁻¹ spectral resolution over the full Raman range in a single scan, with optional 1.5 cm⁻¹ high-resolution gratings. Submicron spatial resolution and rapid mapping enable detailed imaging of delicate samples at low laser power. FlexFocus allows seamless switching between high confocality for depth profiling and high throughput for bulk analysis. Demonstrated applications include silicon crystallinity mapping on solar cells, polymorph detection in pharmaceuticals, polymer laminate depth profiling, and particle analysis in liquid-filled syringes.

Benefits and Practical Applications

• Routine QC: automated instrument qualification, intuitive video-guided workflows, and reproducible spectral accuracy
• Pharmaceuticals: chemical imaging of active ingredients, excipients, and polymorphic forms in tablets
• Life science: non-destructive analysis of tissues and cells under low-power conditions
• Materials research: coatings, interfaces, and laminate structures with high spatial resolution
• Extended versatility: hybrid coupling with FT-Raman (1064 nm), fiber-optic probes, heating/cooling stages, and open-architecture configurations

Future Trends and Opportunities

Advancements may include integration of machine-learning for automated spectral interpretation, real-time chemometric analysis, expanded accessory ecosystems (e.g., advanced microscopy modalities), and deeper infrared excitation to further mitigate fluorescence and broaden sample compatibility.

Conclusion

SENTERRA II sets a new standard for compact Raman microscopy by combining high performance, automation, regulatory compliance, and a user-centric interface, meeting the needs of both high‐throughput quality control and cutting-edge research applications.