Going Green

Importance of the Topic

Human activities now dominate environmental change in the Anthropocene, placing water systems under severe pressure from diverse pollutants. Detecting and quantifying contaminants at trace levels is critical to safeguard ecosystems, public health and future water security. Robust monitoring, accurate interpretation of pollutant behaviour and effective management strategies together form the three pillars of environmental water protection.

Objectives and Overview

This article presents six case studies illustrating how innovative analytical and treatment methods are applied to monitor, understand and manage environmental water pollutants. The studies cover pharmaceutical residues in wastewater sludge, heavy metal profiling, nitrogen isotope tracing in agroecosystems, seasonal behaviour of endocrine disruptors, marine aerosol composition in polar regions and advanced remediation technologies using activated carbon and microbial bioremediation. Each example highlights challenges in detection, insight into pollutant fate and practical solutions for improving water quality.

Methodology

The featured research combines sampling, extraction, separation and detection techniques tailored to specific pollutant classes:

• Solid-phase extraction to probe adsorption mechanisms between pharmaceuticals and sludge solids
• Cloud point extraction for preconcentration of metal chelates prior to flame absorption analysis
• Stable isotope labelling with 15N to trace nitrogen metabolism in plant–pest interactions
• Gas chromatography–mass spectrometry for ultra-trace detection of estrogenic compounds across seasons
• Liquid chromatography–mass spectrometry to quantify organic micropollutants before and after activated carbon filtration
• Atomic force microscopy and modelling to characterise microbial adhesion in bioremediation
• Ultrasonic extraction and triple quadrupole mass spectrometry for amino acid profiling in marine aerosol samples

Instrumentation Used

• Solid-phase extraction (SPE)
• Cloud point extraction (CPE)
• Flame atomic absorption spectrometry (FAAS)
• Gas chromatography–mass spectrometry (GC-MS)
• Liquid chromatography–mass spectrometry (LC-MS)
• Ultra-performance liquid chromatography–PDA-ESI-MS
• Triple quadrupole mass spectrometry (TQMS)
• Atomic force microscopy (AFM)
• Ultrasonic extraction in ultra-pure water

Main Results and Discussion

1. Pharmaceutical–Sludge Interactions: SPE studies revealed that ion exchange, π–π interactions and hydrogen bonding contribute significantly to API sorption, improving predictions of pharmaceutical fate in treatment plants.

2. Heavy Metal Profiling: Cloud point extraction coupled with FAAS enabled simultaneous detection of silver, cadmium, nickel, cobalt and lead at µg/L levels, mapping spatial variability linked to industrial discharges and natural dilution.

3. Nitrogen Metabolism Tracing: 15N labelling in rapeseed–broomrape systems identified glyphosate’s mode of action on parasite amino acid synthesis and established baseline nitrogen flux measurements.

4. Seasonal EDC Variations: GC-MS monitoring demonstrated that removal efficiencies for estrogenic chemicals in wastewater treatment fluctuate with seasonal microbial activity and temperature, suggesting tailored process optimisation.

5. Marine Aerosol Amino Acids: Antarctic sampling and TQMS analysis showed hydrophilic amino acids dominate sea spray aerosols, informing models of cloud nucleation and climate impacts.

6. Remediation Technologies: Pilot trials of powdered versus granular activated carbon highlighted PAC’s superior removal of organic micropollutants due to increased surface area and contact time. Biomechanical studies of Rhodococcus with AFM revealed pH-dependent adhesion properties that can be tuned for efficient hydrocarbon bioremediation.

Benefits and Practical Applications

The discussed methods enhance risk assessment accuracy, support regulatory compliance, optimise wastewater treatment processes and guide the design of sustainable remediation strategies. Improved pollutant profiling informs targeted interventions, while bio-based and adsorption technologies offer scalable solutions for diverse contamination scenarios.

Future Trends and Opportunities

Emerging areas include integration of real-time sensor networks, machine learning for predictive modelling of pollutant fate, development of advanced biofilms for targeted biodegradation and adoption of green analytical protocols. Continued interdisciplinary collaboration will accelerate the translation of laboratory advances into field-deployable systems.

Conclusion

Effective management of environmental water pollution relies on a cycle of precise monitoring, detailed understanding of pollutant behaviour and evidence-based remediation. Innovations in analytical chemistry, combined with high-quality reagents and instrumentation, underpin progress toward resilient water treatment and protection of ecosystems.

References

• Arndt K. et al., 1998. 15N investigation into nitrogen metabolism of Tetrahymena pyriformis. Environmental Health Perspectives, 106(8), 493.
• Barbaro E. et al., 2014. Amino acids in Antarctica: evolution and fate of marine aerosols. Atmospheric Chemistry and Physics Discussions, 14(11), 17067.
• Belhaj D. et al., 2015. Fate of selected estrogenic hormones in an urban sewage treatment plant in Tunisia. Science of The Total Environment, 505, 154.
• Berthod L., Roberts G. & Mills G., 2014. A solid-phase extraction approach for pharmaceutical–sludge interactions. Journal of Pharmaceutical Analysis, 4(2), 117.
• Berthod L. et al., 2017. Effect of sewage sludge type on pharmaceutical partitioning behaviour. Environmental Science: Water Research & Technology, 2, 154.
• Dor E. et al., 2017. Effects of herbicides targeting amino acid biosynthesis in broomrape. Frontiers in Plant Science, 8:707.
• Gadupudi C.K. et al., 2019. Endocrine disrupting compound removal methods in UK wastewater. Sci., 1, 15.
• Gaudin Z. et al., 2014. 15N tracing of amino acid metabolism via UPLC-MS. Analytical Chemistry, 86(2), 1138.
• Karmakar R.N., 2010. Forensic Medicine and Toxicology. Academic Publishers.
• Krivoruchko A. et al., 2019. Advanced Rhodococcus biocatalysts for biotechnologies. Catalysts, 9, 236.
• Kuyukina M. & Ivshina I., 2010. Application of Rhodococcus in bioremediation. In Biology of Rhodococcus. Microbiology Monographs.
• Meinel F. et al., 2014. Pilot-scale investigation of micropollutant removal with activated carbon. Water, Air, & Soil Pollution, 226(1), 1.
• Meinel F. et al., 2016. PAC recirculation for micropollutant removal. Water Science & Technology, 74(4), 927.
• Naeemullah K. et al., 2014. Determination of silver and heavy metals via cloud point extraction. Arabian Journal of Chemistry, 9(1), 105.
• Pen Y. et al., 2015. Effect of EPS on mechanical properties of Rhodococcus. Biochimica et Biophysica Acta, 1848(2), 518.
• Sadat S.M. et al., 2018. Biomonitoring of multiple metals in occupational exposure. Journal of Occupational and Environmental Hygiene, 15(12), 833.
• Schiffer J.M. et al., 2018. Sea spray aerosol: marine biology meets atmospheric chemistry. ACS Central Science, 4, 1617.