1. KONFERENCE ČESKÉ SPOLEČNOSTI PRO HMOTNOSTNÍ SPEKTROMETRII - Sborník abstraktů

Significance of the Topic

Advances in mass spectrometry continue to drive major progress across analytical chemistry, biotechnology, pharmaceutical research, clinical diagnostics and environmental monitoring. The 1st Conference of the Czech Society for Mass Spectrometry brought together experts in gas and liquid phase ionization, imaging, metabolomics, proteomics and instrumentation development to showcase novel methods for sensitive, high-throughput and structure-specific analysis of biomolecules, small molecules, metals and materials.

Objectives and Overview of the Conference

The meeting addressed key goals:

• Expand applications of MALDI, ESI, APCI and emerging ambient ionization techniques (DESI, nanoDESI, SIFT-MS).
• Share innovations in tandem MS, high-energy CID, FT-ICR and high-resolution TOF instruments.
• Demonstrate workflows for lipidomics, metabolomics, proteomics and clinical analysis.
• Enable cross-disciplinary exchange on sample preparation, separations (HPLC, UHPLC, GC, CE, microfluidics) and data processing.

Methodologies and Instrumentation

Contributors reported on a broad array of analytical strategies including:

• MALDI-TOF(/TOF) and LDI imaging for tissue and surface mapping.
• ESI-MS, ESI-Q-TOF, nanoelectrospray and SIFT-MS for real-time gas analysis.
• APCI and CID workflows for structural elucidation of lipids, phospholipids and organometallic species.
• FT-ICR, Orbitrap and high-resolution TOF for accurate-mass fingerprinting and speciation.
• HPLC, UHPLC, GC–MS, GC×GC–TOF, CE–MS and microfluidic interfaces for complex mixture separation.
• Top-down and bottom-up proteomic schemes, surface capture and cross-linking to probe protein structure and interactions.

Main Results and Discussion

Highlights ranged from novel MALDI target coatings enabling on-target oxidation reactions for double-bond localization to ambient DESI/nanoDESI imaging of cuticular lipids on insects. New ligand exchange studies on palladium clusters, detailed mechanistic insights into electron-based dissociations of peptide cation radicals, and advanced HPLC methods for oxidative and structural lipid isomer discrimination were presented. High-throughput clinical assays for tyrosine kinase inhibitors, breath-based SIFT-MS workflows for human health monitoring, and GC×GC metabolomic profiling for inherited metabolic disease diagnostics illustrated direct biomedical impact.

Benefits and Practical Applications

The methods presented demonstrated clear advantages:

• Rapid microbial identification by MALDI Biotyper systems in clinical microbiology.
• Improved lipid and glycan isomer separation by argentation and ion trap HCD.
• Fast therapeutic drug monitoring via flow injection LC-MS/MS and microextraction protocols.
• Ambient surface analysis for forensics, food safety and biomarker discovery.
• Advanced imaging to map lipid, peptide and xenobiotic distributions in tissues.

Future Trends and Opportunities

Key emerging directions include:

• Integration of high-resolution, accurate-mass instruments with ultrafast lasers for real-time imaging.
• Expansion of ambient ionization to single-cell and droplet-based microfluidic workflows.
• Development of robust in-situ derivatization and on-target enrichment for labile analytes.
• Machine learning for automated spectral interpretation and biomarker selection.
• Deeper integration of multi-omics (proteomics, metabolomics, lipidomics) in clinical diagnostics.

Conclusion

The 1st Conference of the Czech Society for Mass Spectrometry highlighted the breadth of current MS innovations and fostered collaboration across academia, industry and healthcare. The novel workflows, instrumentation developments and interdisciplinary applications presented set the stage for continued advances in sensitivity, speed and structural insight, ultimately translating into improved research outcomes and real-world impact.

Použitá instrumentace

• MALDI-TOF and MALDI-TOF/TOF (Bruker, Thermo, Waters) with DHB, CHCA and specialized nanocoatings.
• ESI-Q-TOF and ESI-FT-ICR platforms for accurate-mass measurements.
• Orbitrap and high-resolution TOF analyzers for sub-ppm mass accuracy.
• Triple quadrupole instruments for SRM/MRM clinical assays.
• GC–MS, GC×GC–TOF, HPLC-APCI, HPLC-ESI, CE–MS, UHPLC–MS and microfluidic interfaces.
• Ion traps, ion mobility spectrometers, and cryogenic/secondary emission detectors.
• SIFT-MS, DESI, nanoDESI and on-target phosphopeptide capture systems.

Reference

Please refer to individual abstracts for detailed literature citations.