Biotage Horizon 5000

Importance of the Topic

The Biotage Horizon 5000 solid phase extraction platform streamlines sample preparation workflows in environmental, pharmaceutical and food analysis laboratories. Automation reduces manual errors, increases throughput and ensures reproducible recoveries.

Objectives and Overview

This document provides detailed specifications, environmental requirements and ordering information for the Biotage Horizon 5000 system. It is intended to guide procurement, installation and method development for high-throughput SPE applications.

Methodology and Instrumentation

The system automates conditioning, loading, washing and elution of SPE disks. Up to four extractor modules are controlled by a dedicated PC. Solvent selection and waste management are configured per method requirements. Optional nitrogen blanketing and exhaust kits enhance sample integrity and operator safety.

Used Instrumentation

The core components and accessories include:

• Biotage Horizon 5000 extractor modules with three stations each
• Controller PC running Windows 7 Professional, Intel Core i3 or equivalent
• Solvent valves, waste and water lines, and 20 L waste containers
• Optional nitrogen supply kit, exhaust kit and vapor shield
• Disk holders (47 mm, 90 mm and Fast Flow configurations)
• Elution vessels (Erlenmeyer flasks, VOA adapters)
• Prefilters, mesh screens and carbon cartridges

Key Specifications and Discussion

The system’s footprint is 48.3 × 49.9 × 53.3 cm and it weighs 28.0 kg. It operates on 100–240 VAC, 50–60 Hz, drawing up to 300 W. Environmental conditions of 17–28 °C and 10–70 % non-condensing humidity are required. A regulated nitrogen source (60–80 PSI) can be used to prevent oxidation. PC requirements include 4 GB RAM, 250 GB HDD, HD display and Ethernet connectivity. Compatible solvents cover methylene chloride, ethyl acetate, hexane, methanol, acetone and reagent water. Two waste containers handle aqueous and organic effluents.

Benefits and Practical Applications

Automation enhances reproducibility and frees analyst time for data interpretation. Modular design allows scaling from single modules to multi-module arrays. Compatibility with common disk formats and consumables ensures flexibility across method types. Optional accessories reduce cross-contamination and support inert atmospheres for sensitive analytes.

Future Trends and Applications

Next-generation workflows may integrate the Horizon 5000 with online chromatography or mass spectrometry, enabling seamless end-to-end analysis. Advances in smart user interfaces, remote monitoring and green solvent protocols will further optimize throughput and sustainability.

Conclusion

The Biotage Horizon 5000 offers a robust, flexible and scalable solution for automated SPE. Its comprehensive accessory portfolio and clear specifications support rapid deployment in diverse analytical environments.

Reference

Literature Number PPS529.v1