Biotage VacMaster Disk - User Manual

Importance of the Topic

The Biotage VacMaster Disk system provides an efficient platform for high-throughput solid phase extraction (SPE) of aqueous and viscous samples in environmental, industrial, and analytical laboratories. By applying controlled vacuum across SPE disks, the system streamlines sample preparation workflows, enhances reproducibility, and supports a wide range of solvent compatibilities. Its modular design and adaptability to multiple disk formats address the growing demand for reliable, scalable sample cleanup in trace analysis and quality control.

Objectives and Overview

This user manual outlines the specifications, safe handling practices, setup procedures, operation steps, and maintenance guidelines for the Biotage VacMaster Disk extraction unit. It aims to guide analysts through system installation, vacuum source connection, disk conditioning, sample loading, elution protocols, and routine cleaning of components.

Methodology and Instrumentation

• VacMaster Disk unit constructed from UHMWPE, brass, polypropylene, glass, Viton, aluminum, and polyethylene.
• Compatibility with 47 mm, 90 mm, and 65 mm SPE disks as well as disposable Atlantic ReadyDisks and DryDisk assemblies.
• Integration of a four-way valve with segregated waste ports for aqueous, chlorinated, and organic streams connected to carboy assemblies.
• Vacuum pump (110 V or 220 V) coupled via a vacuum regulator to control flow rates across disks.
• Optional accessories including prefilters, disk holder assemblies, collection chambers, multi-funnels, and vial adapters.

Main Features and Discussion

The VacMaster Disk design supports up to eight units on a single vacuum source for increased throughput. The four-way valve allows seamless transition between conditioning, sample loading, and elution steps while preventing cross-contamination. Segregated waste channels reduce solvent incompatibility risks. Material selection ensures compatibility with a broad solvent range from water and acidified solutions to nonpolar organics like hexane and chloroform.

Benefits and Practical Applications

• Scalable workflow for environmental monitoring of water pollutants and oil and grease analysis.
• Versatile disk chemistries (C18, HLB, DVB, One Pass, high-capacity sorbents) tailored to diverse analytes.
• Easy cleaning and maintenance procedures minimize downtime and extend component lifespan.
• Modular accessories optimize sample handling from initial filtration to final elution into vials.
• Consistent performance through regulated vacuum control and standardized disk conditioning.

Future Trends and Potential Uses

Ongoing developments in membrane technology and novel sorbent materials are expected to shorten sample preparation times and improve analyte recovery. Integration with automated liquid handling and online SPE-LC systems will broaden the VacMaster Disk’s role in high-throughput screening and process analytics. The adoption of greener solvents and fully disposable disk formats may further enhance the environmental sustainability of SPE workflows.

Conclusion

The Biotage VacMaster Disk system offers a robust, flexible, and efficient solution for solid phase extraction in environmental, industrial, and research laboratories. Its configurable waste management, broad solvent compatibility, and comprehensive accessory suite enable reliable sample preparation for trace analysis and quality control. Adherence to recommended safety practices and maintenance procedures ensures consistent performance and longevity of the system.