Rapeseed Oil

Význam tématu

Fatty acid methyl esters (FAMEs) profiling is fundamental for quality control in food, feed and biofuel industries. Accurate separation and identification of individual fatty acids supports nutritional labeling, authenticity testing and process optimization. Gas chromatography with capillary columns remains a cornerstone technique for this purpose.

Cíle a přehled studie

This application note demonstrates the performance of a 25 m Bonded Carbowax (007-CW) column for resolving common FAME components in rapeseed oil. The goal is to achieve baseline separation of saturated and unsaturated C14–C24 methyl esters under a simple temperature program, enabling routine analysis in quality assurance laboratories.

Použitá instrumentace

• Column: 25 m × 0.25 mm I.D. × 0.25 µm bonded carbowax (007-CW)
• Oven program: 200 °C initial, ramp 2 °C/min to 240 °C
• Injector temperature: 250 °C
• Detector: FID at 250 °C
• Carrier gas: Helium at 28 cm/s linear velocity

Metodika

Rapeseed oil was transesterified to FAMEs and injected in split mode. A slow temperature ramp separated C14:0 through C24:0 methyl esters, including mono- and polyunsaturated species. Retention times were recorded and peak resolution assessed.

Hlavní výsledky a diskuse

The method achieved clear separation of eleven FAMEs: C14:0, C16:0, C18:0, C18:1, C18:2, C18:3, C20:0, C20:1, C22:0, C22:1 and C24:0. Saturated and unsaturated pairs were baseline resolved. The carbowax phase provided high selectivity for cis-double bonds, facilitating distinction of linoleic (C18:2) and linolenic (C18:3) esters.

Přínosy a praktické využití metody

• Reliable quantification of nutritional fatty acids in edible oils
• Quality control in biodiesel production by monitoring long-chain esters
• Rapid adoption in QC labs due to simple temperature program and widely available FID detection

Budoucí trendy a možnosti využití

Integration with mass spectrometry could enhance identification of minor isomers. Faster temperature programming and narrower bore columns may reduce analysis time. Automated data processing can streamline high-throughput screening across diverse oil matrices.

Závěr

The 007-CW bonded carbowax column reliably separates key FAMEs from rapeseed oil under moderate temperature conditions. The method supports routine analysis for food and biofuel applications, offering reproducible retention and clear resolution of saturated and unsaturated esters.

Reference

Quadrex Corporation. GC Capillary Column Applications: FAMEs. Woodbridge, CT.