<p><strong>Symposium &quot;Advances in Chromatography and Electrophoresis &amp; Chiranal 2022&quot; belongs to the periodical meetings of specialists working in the field of separation methods.</strong></p>
<p>The biennial conference is organized by Department of Analytical Chemistry, Faculty of Science, <a href="https://lcms.cz/companies/17"><strong>Palacky University</strong></a> in cooperation with Regional Centre of Advanced Technologies and Materials, Czech Chemical Society and <a href="https://lcms.cz/companies/21"><strong>Czech Society for Mass Spectrometry</strong></a>. It is organized as a meeting of the scientists from the universities, academic institutes and experts from the routine analytical laboratories.</p>
<p>The scientific program will deal with all areas of separation science <strong>from fundamental to applied reseach</strong> in chemistry, physics, biology, medicine, forensic science and related disciplines. Official languages of the conference are <strong>English</strong>, <strong>Czech</strong> and <strong>Slovak</strong>.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UPOL_Katedra_analyticke_chemie_Pr_F_UP_Advances_in_Chromatography_and_Electrophoresis_and_Chiranal_2022_Programme_5817ff6a89/UPOL-Katedra-analyticke-chemie-PrF-UP-Advances-in-Chromatography-and-Electrophoresis-and-Chiranal-2022-Programme.webp" alt="UPOL: Katedra analytické chemie PřF UP - Advances in Chromatography and Electrophoresis &amp; Chiranal 2022 - Programme"/></p>
<h3><strong>Programme</strong></h3>
<h3><strong>Monday 13th June</strong></h3>
<h5><strong>14:00 – 17:30 Registration</strong></h5>
<ul>
<li>Fort Science, foyer, ground floor</li>
<li>Individual arrival of participants and accommodation</li>
</ul>
<h5><strong>16:00 – 16:15 Opening of conference</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
<li>Presence of official representatives of Palacký University and Czech Chemical Society</li>
<li><strong>Foreword: Petr Bednář</strong></li>
</ul>
<h5><strong>16:15 – 17:15 Plenary Lecture</strong> (Chairmen: <a href="https://lcms.cz/articles?search=Karel+Lemr">Karel Lemr</a>, Juraj Ševčík)</h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<h5><strong>Innovations in microchip electrophoresis: from design to applications. Honoring the memory of Prof. Milan Hutta</strong></h5>
<ul>
<li><strong>Marián Masár</strong></li>
</ul>
<h5><strong>17:30 – ?? Welcome party + banquet</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<h3><strong>Tuesday 14th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
<li>Installation of posters (Faculty of Science, seminary rooms, 2nd floor)</li>
</ul>
<h5><strong>09:00 – 11:00 Lectures I: Electromigration methods and chip technologies</strong> (Chairmen: Michael Lämmerhofer, Petr Bednář)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Electrokinetic and microchip design for proteomics, interactomics and diagnostics</strong></h5>
<ul>
<li><strong>Anne Varenne</strong></li>
</ul>
<h5><strong>09:30 – 10:00 Non-invasive samples in clinical diagnostics: Can capillary electrophoresis be a suitable diagnostic tool?</strong></h5>
<ul>
<li><strong>Petr Kubáň</strong></li>
</ul>
<h5><strong>10:00 – 10:20 Chiral capillary electrophoresis with diquats as both passive and active players in the enantioseparation games</strong></h5>
<ul>
<li><strong>Václav Kašička</strong></li>
</ul>
<h5><strong>10:20 – 10:40 Simul 6 – A fast simulation software for electrophoresis</strong></h5>
<ul>
<li><strong>Bohuslav Gaš</strong></li>
</ul>
<h5><strong>10:40 – 11:00 Monolithic silicas in high performance liquid chromatography – unique material with lots of benefits</strong></h5>
<ul>
<li><strong>Egidijus Machtejevas</strong> (Merck)</li>
</ul>
<h5><strong>11:00 – 11:20 How we had fun during the lockdown, challenges that new products brought</strong></h5>
<ul>
<li><strong>Luděk Vlk</strong> (Chromservis)</li>
</ul>
<h5><strong>11:20 – 11:40 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>11:40 – 13:40 Lectures II: Multidimensional chromatography, mass spectrometry of small molecules</strong> (Chairmen: Petr Kubáň, Petr Barták)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>11:40 – 12:10 Multidimensional liquid chromatography and pharmaceutical applications</strong></h5>
<ul>
<li><strong>Michael Lämmerhofer</strong></li>
</ul>
<h5><strong>12:10 – 12:40 Novel approaches to overcome dilution in comprehensive two-dimensional liquid chromatography</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Petr+%C4%8Cesla"><strong>Petr Česla</strong></a></li>
</ul>
<h5><strong>12:40 – 13:00 Application of flow-modulated comprehensive two-dimensional gas chromatography in chiral analysis of wine</strong></h5>
<ul>
<li><strong>Olga Vyviurska</strong></li>
</ul>
<h5><strong>13:00 – 13:20 Pharmaceutical analysis in the development of water-soluble prodrug of ICRF–193, a protective agent against anthracycline cardiotoxicity</strong></h5>
<ul>
<li><strong>Petra Štěrbová-Kovaříková</strong></li>
</ul>
<h5><strong>13:20 – 13:40 Achieving robust profiling, sensitive quantitation and confident unknown identification for small molecules applications with <a href="https://lcms.cz/products/403">Orbitrap Exploris 120 MS</a></strong></h5>
<ul>
<li><strong>Maciej Bromirski</strong> (<a href="https://lcms.cz/companies/42">Pragolab</a>)</li>
</ul>
<h5><strong>13:40 – 14:40 Lunch</strong></h5>
<ul>
<li>Mensa</li>
</ul>
<h5><strong>14:40 – 16:00 Poster session I (odd poster numbers) and coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h5><strong>16:00 – 17:50 Lectures III: Current trends in chromatography</strong> (Chairmen: Václav Kašička, Ondřej Kurka)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>16:00 – 16:30 Comparison of lipid class and lipid species separation approaches in the lipidomic analysis</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Michal+Hol%C4%8Dapek"><strong>Michal Holčapek</strong></a></li>
</ul>
<h5><strong>16:30 – 17:00 Miniaturization as one of the trends in modern analytical chemistry</strong></h5>
<ul>
<li><strong>Zeineb Aturki</strong></li>
</ul>
<h5><strong>17:00 – 17:20 The possibilities of chiral separation of anionic boron clusters</strong></h5>
<ul>
<li><strong>Radim Kučera</strong></li>
</ul>
<h5><strong>17:20 – 17:40 Electron activated dissociation as potential solution to identification and quantitation of isomers using mass spectrometry without additional separation techniques</strong></h5>
<ul>
<li><strong>Tomáš Korba</strong> (Amedis)</li>
</ul>
<h5><strong>17:40 – 18:00 HPLC separation in metabolomic studies: challenges and solutions</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Ond%C5%99ej+Lacina"><strong>Ondřej Lacina</strong></a> (<a href="https://lcms.cz/companies/37">HPST</a>)</li>
</ul>
<h5><strong>18:00 – 19:00 Dinner</strong></h5>
<ul>
<li>Mensa</li>
</ul>
<h5><strong>19:30 – 21:00 Informal evening visit to the laboratories of the Department of Analytical Chemistry – including the Cyclic ion mobility MS lab</strong></h5>
<ul>
<li><a href="https://lcms.cz/products/202"><strong>SELECT SERIES Cyclic IMS instrument</strong></a>, third installed instrument with this unique technology worldwide</li>
</ul>
<h3><strong>Wednesday 15th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>09:00 – 11:20 Lectures IV:  Applications and practice of chromatography, heritage science, forensic science</strong> (Chairmen: Radim Kučera, Lukáš Kučera)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Art of chromatography – Chromatography in art</strong></h5>
<ul>
<li><strong>Václav Pitthard</strong></li>
</ul>
<h5><strong>09:30 – 10:20 Forensic applications of analytical chemistry</strong></h5>
<ul>
<li><strong>Luboš Valerián, Jan Aufart</strong></li>
</ul>
<h5><strong>10:20 – 10:40 Cyclic ion mobility – mass spectrometry of isomeric cathinones</strong></h5>
<ul>
<li><strong>Marianna Nytka</strong></li>
</ul>
<h5><strong>10:40 – 11:00 Intact protein analysis in biological fluids by liquid chromatography – triple quadrupole mass spectrometry</strong></h5>
<ul>
<li><strong>Katarína Maráková</strong></li>
</ul>
<h5><strong>11:00 – 11:20 Practical approaches for identifying equivalent and orthogonal columns</strong></h5>
<ul>
<li><strong>Heiko Behr</strong> (Phenomenex)</li>
</ul>
<h5><strong>11:20 – 13:00 Poster session II and coffee break</strong></h5>
<ul>
<li>Even poster numbers</li>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h5><strong>13:00 – 14:00 Lunch</strong></h5>
<ul>
<li>Mensa</li>
</ul>
<h5><strong>15:00 – 17:00 Social/sport event</strong></h5>
<ul>
<li>Bowling at Bowland Olomouc</li>
<li>A tour of Regional Office of National Heritage Institute (see details at the bottom of the page)</li>
</ul>
<h5><strong>18:00 – ?? Social dinner</strong></h5>
<ul>
<li>Faculty of Science, foyer, 6th floor</li>
</ul>
<h3><strong>Thursday 16th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>09:00 – 10:30 Lectures V: Supercritical fluid chromatography, pharmaceutical analysis</strong> (Chairmen: Dalibor Šatínský, Petr Fryčák)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Supercritical fluid chromatography mass spectrometry coupling: where are we?</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Lucie+Nov%C3%A1kov%C3%A1"><strong>Lucie Nováková</strong></a></li>
</ul>
<h5><strong>09:30 – 10:00 Reaction of basic analytes with carbon dioxide under supercritical fluid chromatography conditions: Confirmed</strong></h5>
<ul>
<li><strong>Michal Kohout</strong></li>
</ul>
<h5><strong>10:00 – 10:20 Unified chromatography – a step towards universal chromatographic methods</strong></h5>
<ul>
<li><strong>Volodymyr Pauk</strong></li>
</ul>
<h5><strong>10:20 – 10:40 Control of N-Nitrosamines in Pharmaceuticals</strong></h5>
<ul>
<li><strong>Jakub Jireš</strong></li>
</ul>
<h5><strong>10:40 – 11:00 Non-targeted UHPLC-DIA-HRMS approach for examination of impurities in levothyroxine formulations</strong></h5>
<ul>
<li><strong>Hana Chmelařová</strong></li>
</ul>
<h5><strong>11:00 – 11:20 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>11:20 – 13:30 Lectures VI: Novelty in sample preparation, ion mobility, data treatment</strong> (Chairmen: Lucie Nováková, Volodymyr Pauk)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor)</li>
</ul>
<h5><strong>11:20 – 11:50 Advanced extraction approaches to sample extraction in flow and chromatography techniques based on nanofibers</strong></h5>
<ul>
<li><strong>Dalibor Šatínský</strong></li>
</ul>
<h5><strong>11:50 – 12:10 DisperSpin solid phase micro-extraction as a novel tool of phytohormonal analysis</strong></h5>
<ul>
<li><strong>Ivan Petřík</strong></li>
</ul>
<h5><strong>12:10 – 12:30 Monitoring of PAHs in simulated natural and artificial fires by HPLC-DAD-FLD with the application of Multi Component Integrated Calibration Method to improve quality of analytical results</strong></h5>
<ul>
<li><strong>Paweł Świt</strong></li>
</ul>
<h5><strong>12:30 – 12:50 Ion-mobility coupling with desorption electrospray ionization mass spectrometry imaging in plant isomer research</strong></h5>
<ul>
<li><strong>Chao Zhang</strong></li>
</ul>
<h5><strong>12:50 – 13:10 High resolution ion mobility separation</strong></h5>
<ul>
<li><strong>Alexandr Muck</strong> (<a href="https://lcms.cz/companies/50">Waters</a>)</li>
</ul>
<h5><strong>13:10 – 13:30 Using trapped ion mobility spectrometry to improve confidence in identification of knowns and unknowns</strong></h5>
<ul>
<li><strong>Noud van der Borg</strong> (Bruker)</li>
</ul>
<h5><strong>13:30 – 14:10 Closing of conference and farewell drink</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>14:10 – 15:10 Lunch</strong></h5>
<ul>
<li>Mensa</li>
</ul>
<h4><strong>Social programme:</strong></h4>
<h5><strong>A tour of Regional Office of National Heritage Institute</strong></h5>
<p>The Archaeological Department of the National Heritage Institute, Regional Office in Olomouc has been carrying out excavations in the territory of the former capital of Moravia for 50 years. In recent years, interdisciplinary cooperation with natural scientists – archaeobotanists, palynologists, anthropologists and, last but not least, chemists from the Faculty of Science of Palacký University – has been successfully developing. Examples of jointly researched artefacts include a prehistoric depot from the site of Benkov near Uničov, medieval silk textiles from Křížkovského Street in Olomouc or small objects of daily devotion in the modern period from the same research.</p>
<h5><strong>Sporting event:</strong></h5>
<p>Bowling in BOWLAND Bowling Center, Šantovka</p>


Advances in Chromatography and Electrophoresis & Chiranal 2022 - Programme

<p><strong>The biennial conference is organized by Department of Analytical Chemistry, Faculty of Science, Palacky University in cooperation with Czech Chemical Society and Czech Society for Mass Spectrometry. It is organized as a meeting of the scientists from the universities, academic institutes and experts from the routine analytical laboratories.</strong></p>
<p>The scientific program will deal with all areas of separation science from fundamental to applied reseach in chemistry, physics, biology, medicine, forensic science and related disciplines. Official languages of the conference are English, Czech and Slovak.</p>
<h3><strong>Registration</strong></h3>
<blockquote>
<p><a href="https://chiranal2024.upol.cz/index.php?link=registration"><strong>Registration</strong></a></p>
</blockquote>
<p>Registration fee is 5000,- CZK (200 EUR) + tax 21 %.</p>
<p>Registration covers printed Program and Book of Abstracts, access to all scientific and social events held in the framework of the conference and meals.</p>
<p>The invoice should be paid until <strong>May 26th, 2024</strong>.</p>
<h3><strong>Programme</strong></h3>
<h3><strong>Monday 17th June</strong></h3>
<h5><strong>14:00 – 17:30 Registration</strong></h5>
<ul>
<li>Fort Science (foyer, ground floor), individual arrival of participants and accommodation</li>
</ul>
<h5><strong>16:00 – 16:15 Opening of the conference (Fort Science, Laudon Hall, ground floor), presence of official representatives of the conference partners</strong></h5>
<ul>
<li>Foreword: Petr Bednář</li>
</ul>
<h5><strong>16:15 – 17:15 Plenary Lecture</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<h5><strong>Toward unrivaled chromatographic resolving power: Design and development of comprehensive spatial three-dimensional liquid-phase separation technology</strong></h5>
<ul>
<li>Sebastiaan Eeltink</li>
</ul>
<h5><strong>17:30 – 17:45 Ceremonial launch of the book of Lucie Nováková, Michal Douša, Petr Česla and Jiří Urban: Modern HPLC separations in theory and practice</strong></h5>
<h5><strong>17:45 – ?? Welcome party + banquet</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UPOL_Chiranal_cover_image_6d8ebc4308/UPOL-Chiranal-cover-image.jpg" alt="Chiranal: Advances in Chromatography and Electrophoresis &amp; Chiranal 2024"/></p>
<h3><strong>Tuesday 18th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
<li>installation of posters (Faculty of Science, seminary rooms, 2nd floor)</li>
</ul>
<h4><strong>09:00 – 11:20 Lectures I: Electromigration techniques, ion exchange chromatography</strong></h4>
<ul>
<li>chairmen: Michael Lämmerhofer, Petr Bednář</li>
<li>Faculty of Science, assembly hall, 2nd floor)</li>
</ul>
<h5><strong>09:00 – 09:30 Electrokinetic methodologies for diagnostics development</strong></h5>
<ul>
<li>Anne Varenne</li>
</ul>
<h5><strong>09:30 – 10:00 Advances in microchip electrophoresis for the analysis of environmental and biological samples</strong></h5>
<ul>
<li>Marián Masár</li>
</ul>
<h5><strong>10:00 – 10:20 Determination of effective charge and ionic mobilities of highly sulfated cyclodextrins by capillary isotachophoresis and zone electrophoresis</strong></h5>
<ul>
<li>Václav Kašička</li>
</ul>
<h5><strong>10:20 – 10:40 Capillary Zone Electrophoresis – Mass spectrometry for intact protein analysis in biological fluids – possibilities and limitations</strong></h5>
<ul>
<li>Katarína Maráková</li>
</ul>
<h5><strong>10:40 – 11:00 Microcontrollers and their application in constructing analytical instrumentation</strong></h5>
<ul>
<li>Petr Kubáň</li>
</ul>
<h5><strong>11:00 – 11:20 Adsorbable organic fluorine (AOF) – a sum parameter for non-targeted screening of per- and polyfluorinated alkyl substances (PFASs) in waters</strong></h5>
<ul>
<li>Jan Soukup (<a href="https://lcms.cz/companies/176"><strong>Metrohm</strong></a>)</li>
</ul>
<h5><strong>11:20 – 11:40 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h4><strong>11:40 – 13:40 Lectures II: Approaches to method development in LC and MS, capillary chromatography</strong></h4>
<ul>
<li>chairmen: Anne Varenne, Petr Barták</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>11:40 – 12:10 How to successfully analyse oligonucleotides with liquid chromatography</strong></h5>
<ul>
<li>Davy Guillarme</li>
</ul>
<h5><strong>12:10 – 12:40 Recent applications of nano-liquid chromatography for the characterization of plant and food products</strong></h5>
<ul>
<li>Zeineb Aturki</li>
</ul>
<h5><strong>12:40 – 13:00 Personal insights on the design of experiments</strong></h5>
<ul>
<li>Jiří Urban</li>
</ul>
<h5><strong>13:00 – 13:20 How to make chromatography more sustainable</strong></h5>
<ul>
<li>Egidijus Machtejevas (Merck)</li>
</ul>
<h5><strong>13:20 – 13:40 Automated workflow for derivatized analyte analysis in LC-TIMS-MS/MS 4D-metabolomics data: Incorporating in-silico</strong></h5>
<ul>
<li>Aiko Barsch (Bruker Daltonics)</li>
</ul>
<h5><strong>13:40 – 14:40 Lunch</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>14:40 – 16:00 Poster session I (odd poster numbers) and coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h4><strong>16:00 – 18:00 Lectures III: Current trends in chromatography</strong></h4>
<ul>
<li>chairmen: Václav Kašička, Ondřej Kurka</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>16:00 – 16:30 Isomer separations in metabolomics and lipidomics</strong></h5>
<ul>
<li>Michael Lämmerhofer</li>
</ul>
<h5><strong>16:30 – 17:00 New Trends in the Lipidomic and Metabolomic Analysis</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Michal+Hol%C4%8Dapek"><strong>Michal Holčapek</strong></a></li>
</ul>
<h5><strong>17:00 – 17:20 Unveiling the Power of Deep UV LAESI/LAESCI Dual Ambient Ionization: Towards Comprehensive Molecular MSI</strong></h5>
<ul>
<li>Barbora Papoušková</li>
</ul>
<h5><strong>17:20 – 17:40 Advanced small molecule mass spectrometry</strong></h5>
<ul>
<li>Luka Milivojevic (<a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a>/Thermo Fisher Scientific)</li>
</ul>
<h5><strong>17:40 – 18:00 Employing ballistic gradients, vacuum jacketed columns and prototype benchtop multi reflecting time-of-flight (MRT) to increase lipidomic analytical throughput whilst maintainig highly confident identifications</strong></h5>
<ul>
<li>Alex Muck (<a href="https://lcms.cz/companies/50"><strong>Waters</strong></a>)</li>
</ul>
<h5><strong>18:00 – 19:00 Dinner</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>19:15 – 21:00 Excursion to the Eureka University Brewery and Department of Analytical Chemistry</strong></h5>
<h3><strong>Wednesday 19th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h4><strong>09:00 – 11:20 Lectures IV: Advances in biomolecules analysis, heritage science</strong></h4>
<ul>
<li>chairmen: Sebastiaan Eeltink, Lukáš Kučera</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Advances in chiral separation of carboranes – new phenyl ring isosteres for drug development</strong></h5>
<ul>
<li>Radim Kučera</li>
</ul>
<h5><strong>09:30 – 10:00 Analysis of selected drugs in whole blood collected with a volumetric absorptive microsampling device</strong></h5>
<ul>
<li>Petra Štěrbová-Kovaříková</li>
</ul>
<h5><strong>10:00 – 10:20 Translation of omics into clinics</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=David+Friedeck%C3%BD"><strong>David Friedecký</strong></a></li>
</ul>
<h5><strong>10:20 – 10:40 Py/GC-MS characterization of oil paintings to correlate museum environment and materials condition over time for SMARTMUS-e project</strong></h5>
<ul>
<li>Paola Lucero</li>
</ul>
<h5><strong>10:40 – 11:00 Recent chromatographic advances in plant hormone profiling methods</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Ond%C5%99ej+Nov%C3%A1k"><strong>Ondřej Novák</strong></a> (on behalf of <a href="https://lcms.cz/companies/37"><strong>Altium</strong></a>)</li>
</ul>
<h5><strong>11:20 – 13:00 Poster session II (even poster numbers) and coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h5><strong>13:00 – 14:00 Lunch</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>15:00 – 17:00 Social/sport event</strong></h5>
<ul>
<li>Bowling at Bowland Olomouc</li>
<li>A tour around Olomouc, visit of Regional Museum in Olomouc</li>
</ul>
<h5><strong>18:00 – ?? Social dinner</strong></h5>
<ul>
<li>Faculty of Science, foyer, 6th floor</li>
</ul>
<h3><strong>Thursday 20th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>09:00 – 11:00 Lectures V: Matrix effects, chiral separation, advances in stationary phases, supercritical fluid chromatography</strong></h5>
<ul>
<li>chairmen: Zeineb Aturki, Petr Fryčák</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Matrix effects in LC-MS: importance of correct calculation approach</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Lucie+Nov%C3%A1kov%C3%A1"><strong>Lucie Nováková</strong></a></li>
</ul>
<h5><strong>09:30 – 10:00 New trends in ion exchange-type chiral stationary phases and their chiral recognition mechanisms</strong></h5>
<ul>
<li><a href="https://lcms.cz/article/3991"><strong>Michal Kohout</strong></a></li>
</ul>
<h5><strong>10:00 – 10:20 Development of an easy-to-set-up multiple heart-cutting achiral-chiral LC-LC method for the analysis of branched-chain amino acids in food supplements</strong></h5>
<ul>
<li>Ghaid W.A. Abualzulof</li>
</ul>
<h5><strong>10:20 – 10:40 Rapid profiling of bioactive compounds using supercritical fluid chromatography coupled with tandem mass spectrometry</strong></h5>
<ul>
<li>Ivan Petřík</li>
</ul>
<h5><strong>10:40 – 11:00 Symbiosis of core-shell and fully porous particles: Implementing both for better HPLC and UHPLC results</strong></h5>
<ul>
<li>Jan Vlasák (<a href="https://lcms.cz/companies/51"><strong>Phenomenex</strong></a>)</li>
</ul>
<h5><strong>11:00 – 11:20 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>11:20 – 13:30 Lectures VI: Selectivity in chromatography and mass spectrometry and its optimization, analysis of natural products</strong></h5>
<ul>
<li>chairmen: Lucie Nováková, <a href="https://lcms.cz/articles?search=Tom%C3%A1%C5%A1+Pluh%C3%A1%C4%8Dek"><strong>Tomáš Pluháček</strong></a></li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>11:20 – 11:50 Targeted and untargeted analysis of psilocybin mushrooms using LC-MS</strong></h5>
<ul>
<li>Kevin Schug</li>
</ul>
<h5><strong>11:50 – 12:10 On the potential of two-dimensional liquid chromatography for environmental analysis</strong></h5>
<ul>
<li>Deirdre Cabooter</li>
</ul>
<h5><strong>12:10 – 12:30 Leveraging ion mobility spectrometry-mass spectrometry data using machine learning reveals provenance of indigo dyes</strong></h5>
<ul>
<li>Volodymyr Pauk</li>
</ul>
<h5><strong>12:30 – 12:50 Numerical optimization of gradient separations in RP and HILIC using non-linear retention models</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Petr+%C4%8Cesla"><strong>Petr Česla</strong></a></li>
</ul>
<h5><strong>12:50 – 13:10 Do we need separations in clinical diagnostics?</strong></h5>
<ul>
<li>Rutuja Patil</li>
</ul>
<h5><strong>13:10 – 13:30 Metabolomics analysis of Moringa oleifera leaves: correlation between in vitro effect on C2C12 myotubes cell line and geographical origin</strong></h5>
<ul>
<li>Mariateresa Maldini (Amedis)</li>
</ul>
<h5><strong>13:30 – 14:10 Closing of the conference and farewell drink (Faculty of Science, foyer, 2nd floor)</strong></h5>
<h5><strong>14:10 – 15:10 Lunch (university cafeteria)</strong></h5>
<h3><strong>Partners and Sponsors</strong></h3>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Chiranal_2024_Partners_e3fc1710b0/Chiranal-2024-Partners.jpg" alt="Chiranal 2024: Partners"/></p>
<ul>
<li><p><strong>Faculty of Science, Palacký University, Olomouc</strong></p>
</li>
<li><p><strong>Department of Analytical Chemistry, Palacký University, Olomouc</strong></p>
</li>
<li><p><strong>Czech Chemical Society</strong></p>
</li>
<li><p><strong>Chromatography &amp; Electrophores Czech Chemical Society</strong></p>
</li>
<li><p><a href="https://lcms.cz/companies/21"><strong>Czech Society for Mass Spectrometry</strong></a></p>
</li>
<li><p><strong>Fort Science</strong></p>
</li>
<li><p><strong>ARTECA</strong></p>
</li>
<li><p><strong>TEVA</strong></p>
</li>
<li><p><a href="https://lcms.labrulez.com/companies/1"><strong>LabRulezLCMS</strong></a></p>
</li>
</ul>
<h3><strong>Sponsors Chiranal 2024</strong></h3>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Chiranal_Sponsors_2024_14389caaaf.jpg" alt="Chiranal: Sponsors 2024"/></p>
<ul>
<li><a href="https://lcms.cz/companies/37"><strong>Altium International</strong></a></li>
<li><strong>Alven Laboratories</strong></li>
<li><strong>AMEDIS</strong>/<strong>SCIEX</strong></li>
<li><strong>Bruker</strong></li>
<li><a href="https://lcms.labrulez.com/companies/168"><strong>ECOM</strong></a></li>
<li><strong>Chromservis</strong></li>
<li><strong>MERCK</strong></li>
<li><a href="https://lcms.cz/companies/176"><strong>Metrohm</strong></a></li>
<li><a href="https://lcms.cz/companies/51"><strong>Phenomenex</strong></a></li>
<li><a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a>/<strong>Thermo Scientific</strong></li>
<li><a href="https://lcms.cz/companies/43"><strong>SHIMADZU</strong></a></li>
<li><strong>Villa Labeco</strong></li>
<li><a href="https://lcms.cz/companies/50"><strong>Waters</strong></a></li>
</ul>
<h3><strong>Permanent scientific committee</strong></h3>
<ul>
<li><p><strong>Daniel W. Armstrong (US)</strong></p>
</li>
<li><p><strong>Petr Barták (CZ)</strong></p>
</li>
<li><p><strong>Petr Bednář (CZ)</strong></p>
</li>
<li><p><strong>Salvatore Fanali (IT)</strong></p>
</li>
<li><p><strong>Bohuslav Gaš (CZ)</strong></p>
</li>
<li><p><strong>Václav Kašička (CZ)</strong></p>
</li>
<li><p><a href="https://lcms.cz/articles?search=Karel+Lemr"><strong>Karel Lemr (CZ)</strong></a></p>
</li>
<li><p><strong>Wolfgang Lindner (AT)</strong></p>
</li>
<li><p><strong>Eva Smolková (CZ)</strong></p>
</li>
<li><p><strong>Zdeněk Stránský (CZ)</strong></p>
</li>
<li><p><strong>Juraj Ševčík (CZ)</strong></p>
</li>
<li><p><a href="https://lcms.cz/articles?search=Franti%C5%A1ek+%C5%A0vec"><strong>František Švec (CZ)</strong></a></p>
</li>
<li><p><strong>Eva Tesařová (CZ)</strong></p>
</li>
</ul>
<h5><strong>Contacts</strong></h5>
<ul>
<li><p><strong>Dr. Ondřej Kurka</strong> (phone: +420 585 634 450, email: ondrej.kurka@upol.cz)</p>
</li>
<li><p><strong>Assoc. Prof. Petr Bednář</strong> (phone: +420 585 634 403, email: petr.bednar@upol.cz)</p>
</li>
</ul>


Advances in Chromatography and Electrophoresis & Chiranal 2024 (Programme)

<p><strong>Symposium "Advances in Chromatography and Electrophoresis &amp; Chiranal" belongs to the periodical meetings of specialists working in the field of separation methods. The biennial conference is organized by Department of Analytical Chemistry, Faculty of Science, Palacky University in cooperation with Czech Chemical Society and Czech Society for Mass Spectrometry. It is organized as a meeting of the scientists from the universities, academic institutes and experts from the routine analytical laboratories.</strong></p><p><a target="_blank" rel="noopener noreferrer" href="https://www.upol.cz/chiranal/programme/"><strong>The scientific program</strong></a> will deal with all areas of separation science from fundamental to applied reseach in chemistry, physics, biology, medicine, forensic science and related disciplines. Official languages of the conference are English, Czech and Slovak.</p><ul><li><strong>Date: </strong>15. - 18. June 2026</li><li><strong>Place:</strong> Olomouc, Czech Republic</li><li><strong>Contact: </strong>prof. RNDr. Petr Bednář, Ph.D. (Department of Analytical Chemistry, Faculty of Science, Palacky University)<ul><li><strong>e-mail: </strong>petr.bednar@upol.cz</li></ul></li></ul><h3><strong>Registration</strong></h3><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://www.upol.cz/chiranal/registration/"><strong>Register for the conference HERE</strong></a></p></blockquote><ul><li><strong>Conference Fees:</strong> 6.000,- CZK / 250 EUR &nbsp;without VAT</li><li><strong>Deadline for registration is May 31st, 2026.</strong></li><li>You will receive a registration confirmation by email.</li></ul><h3><strong>Abstract Submission</strong></h3><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://www.upol.cz/chiranal/abstract-submission/"><strong>Submit your Abstract HERE</strong></a></p></blockquote><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.upol.cz/fileadmin/users/381/template.docx"><strong>Download Abstract Template</strong></a></li><li>The <strong>deadline for submitting</strong> abstracts for <strong>oral presentations</strong> and posters is <strong>May 11, 2026</strong>.</li></ul><h3><strong>Preliminary Programme</strong></h3><h4><strong>Monday 15th June 2026, Fort Science</strong></h4><h5><strong>16:15 - 17:15 Plenary Lecture</strong></h5><ul><li>Michael Laemmerhofer</li></ul><h4><strong>Tuesday 16th June 2026</strong></h4><h5><strong>11:30 - 12:00 Enantioselective Ion Pairing Systems, my 50 years of Contribution</strong></h5><ul><li>Wolfgang Lindner&nbsp;</li></ul><h5><strong>16:30 - 17:00 Data science-assisted method development for on-line supercritical fluid extraction – chromatography</strong></h5><ul><li>Kevin Schug (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/301"><strong>The University of Texas at Arlington</strong></a>)</li></ul><h4><strong>Wednesday 17th June 2026</strong></h4><h5><strong>9:00 – 9:30 The Role of Analytical Chemistry in Solving Contemporary Challenges in Biomethane Plants</strong></h5><ul><li>Justyna Płotka-Wasylka</li></ul><h5><strong>11:30 - 12:00 Rational approaches to MALDI-MS matrix design</strong></h5><ul><li>Hans-Dieter Junker</li></ul><h5><strong>12:50 - 13:10 Using Charge Detection Mass Spectrometry with an Electrostatic Linear Ion Trap for the Analysis of Viral Vectors and Large Protein Complexes</strong></h5><ul><li>Alex Muck (Waters)</li></ul><h4><strong>Thursday 18th June 2026</strong></h4><h5><strong>9:00 – 9:30 Capillary Electrophoresis - Mass Spectrometry for the characterization of proteoforms on the intact level</strong></h5><ul><li>Christian Neusüß</li></ul><h5><strong>9:30 – 10:00 Investigation of acid-base and electromigration properties of biopeptides by capillary electrophoresis</strong></h5><ul><li>Václav Kašička</li></ul><h5><strong>11:30 - 12:00 Open-source capillary electrophoresis - democratizing separation science: Design principles and practical implementations from our laboratory</strong></h5><ul><li>Petr Kubáň</li></ul><h5><strong>12:00 - 12:30 Analysis of biomedical, environmental and pharmaceutical samples by microchip electrophoresis</strong></h5><ul><li>Marián Masár</li></ul><h5><strong>12:30 - 13:00 UV-LED coupling for high-sensitivity glycan analysis in commercial capillary electrophoresis system</strong></h5><ul><li>Jana Lavická</li></ul><h4><strong>Sponsors</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/companies/37"><strong>Altium International s.r.o. (HPST)</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/companies/50"><strong>Waters Gesellschaft m.b.H.</strong></a></li><li>Teva</li></ul><h4><strong>Partners</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/341"><strong>Department of Analytical chemistry, Palacký University Olomouc</strong></a></li><li>Faculty of Science, Palacký University Olomouc</li><li>SMALT (Spojené laboratoře pro materiálové a analytické technologie)</li><li>Czech society for mass spectrometry</li><li>Česká společnost chemická</li><li>ARTECA</li><li>Pevnost poznání</li><li>Odborná skupina chromatografie a elektroforézy ČSCH</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/1"><strong>LabRulez</strong></a></li></ul><h4><strong>Conference site</strong></h4><h5><strong>Address of conference site:</strong></h5><ul><li>Faculty of Science</li><li>Palacky University</li><li>17. listopadu 1192/12</li><li>779 00 Olomouc</li></ul>

Advances in Chromatography and Electrophoresis & Chiranal 2026 - Registration and Abstract submission open!

<p><strong>The 19th Czech - Slovak Spectroscopis Conference (19th CSSC) is a proceeding based on a long-term scientific cooperation between Ioannes Marcus Marci Spectroscopic Society and The Slovak Spectroscopic Society. The meetings organized by the former Czechoslovak spectroscopic society (1949 – 1993) in the past and the continuation in this tradition in the form national Slovak and Czech spectroscopic conferences now, since 2008, exist as a joint event.</strong></p><p>The Czech spectroscopic conference was repeated every four years, while the Slovak spectroscopic conference took place biannually. Joint scientific meetings are now held every two years, alternating between the Czech Republic and Slovakia. The order may change if this conference is combined with the European Symposium on Analytical Spectrometry (ESAS). To maintain the tradition of the original separate national conferences, the conferences held in the Czech Republic retain their original serial numbering, and the same applies to the conferences held in Slovakia.</p><h3><strong>Registration</strong></h3><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://cssc2026.spektroskopie.cz/registration_online"><strong>Online registration form</strong></a></p></blockquote><h4><strong>Registration Fees</strong></h4><h5><strong>Early (before June 15, 2026)</strong></h5><ul><li><strong>Participant (Member of IMMSS and SSS): </strong>10 000 CZK&nbsp;&nbsp;</li><li><strong>Participant (Non-member of IMMSS and SSS): </strong>14 000 CZK&nbsp;&nbsp;&nbsp;</li><li><strong>Students: </strong>7 000 CZK&nbsp;</li><li><strong>1-day registration:</strong> 5 000 CZK</li><li><strong>Accompanying person*: </strong>5 000 CZK</li></ul><h5><strong>Regular (after June 15, 2026)</strong></h5><ul><li><strong>Participant (Member of IMMSS and SSS): </strong>13 000 CZK&nbsp;&nbsp;</li><li><strong>Participant (Non-member of IMMSS and SSS): </strong>18 200 CZK&nbsp;&nbsp;&nbsp;</li><li><strong>Students: </strong>9 100 CZK&nbsp;</li><li><strong>1-day registration:</strong> 6 500 CZK</li><li><strong>Accompanying person*: </strong>6 500 CZK</li></ul><p><i>* The accompanying person does not have access to the scientific programme</i></p><p><strong>The conference fee is calculated with VAT</strong> and includes:</p><ul><li>attendance to lectures and poster session</li><li>book of abstracts (electronic version)</li><li>booklet</li><li>lunches, refreshments (coffee/tea breaks)</li><li>excursion</li><li>attendance at Welcome Party and Gala Dinner.&nbsp;</li></ul><p><strong>For an accompanying person</strong>, the conference fee includes the Welcome Party, the excursion and the Conference Dinner. The Conference Dinner is not included in the 1-day registration. This payment should be arranged with the Organising Committee.</p><p><i><strong>Accommodation is not included in the conference fee.</strong></i></p><h4><strong>TOPICS &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;</strong></h4><h5><strong>Czech - Slovak Spectroscopic Conference</strong></h5><ul><li>Atomic spectrometry (AAS, ICP-MS, AFS, OES, speciation analysis, etc.)</li><li>Molecular spectrometry/spectroscopy (UV-Vis, NMR, IR, Raman, circular dichroism, etc.)</li><li>New techniques and data processing methods in IR and Raman spectroscopy, Special methods of Raman spectroscopy (resonance Raman, SERS, SERRS, TERS)</li><li>XRF spectrometry (EDS, WDS, XRF, PIXE, etc.)</li><li>Mass spectrometry &amp; hyphenated techniques</li><li>Instrumental radioanalytical methods (Gamma spectroscopy, NAA, etc.)</li><li>Laser spectroscopy and special spectroscopy techniques</li><li>Sample preparation and introduction techniques</li><li>Applications &nbsp;of atomic and molecular spectroscopy</li><li>Statistics and metrology in spectroscopy</li></ul><h5><strong>Mössbauer Spectroscopy in Material Science</strong></h5><ul><li>Environmental, catalytic, biologic, medical applications</li><li>Magnetism, magnetic nanoparticles, molecular magnets</li><li>Nanomaterials, nanostructures, nanocomposites, core-shell nanoparticles, thin films</li><li>Nanomaterials in biomedicine</li><li>Nuclear forward scattering in materials science</li><li>Solid-state chemistry and physics</li><li>Industrial applications, corrosion</li><li>Instrumentation, software</li><li>Earth Science, Mineralogy and Archaeology</li></ul><h4><strong>Venue</strong></h4><h5><strong>Faculty of Science</strong></h5><ul><li>Palacky University Olomouc</li><li>17. listopadu 1192/12, &nbsp;Olomouc, Czech Republic</li></ul><h4><strong>Contacts</strong></h4><p><strong>David Milde (Conference Chair) &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</strong> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</p><ul><li>Palacky University Olomouc</li><li><strong>e-mail:</strong> david.milde(at)upol.cz</li></ul><p><strong>Libor Machala (MSMS 2026)</strong></p><ul><li>Palacky University Olomouc</li><li><strong>e-mail:</strong> libor.machala(at)upol.cz</li></ul>

Registration to 19th Czech - Slovak Spectroscopic Conference & Mössbauer Spectroscopy in Material Science

<p><strong>This study introduces atmospheric solids analysis probe high-resolution tandem mass spectrometry with cyclic ion mobility as a novel approach for characterizing Asian lacquers. Applied to 27 historical samples from Japan, China, Vietnam, and Myanmar, the method enabled classification by geographic origin based on phenol and benzenediol derivatives.</strong></p><p>In addition to lacquer identification, the technique simultaneously detected pigments such as arsenic sulfides and associated resins, providing comprehensive chemical insight. This approach expands analytical capabilities for cultural heritage studies, supporting restoration, conservation, and art historical research.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572"><strong>Characterization of Asian lacquers by atmospheric solids analysis probe high resolution tandem mass spectrometry coupled with cyclic ion mobility separation</strong></a></h4><p>Vojtěch Zemek, Radek Ryšánek, Petra Krejčí, Lukáš Kučera, Jana Nádvorníková, Adéla Tůmová, Helena Heroldová, Adriana Stříbrná, Petr Bednář</p><p>Journal of Cultural Heritage, 77, 2026, 187-196</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.culher.2025.11.012">https://doi.org/10.1016/j.culher.2025.11.012</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><div><p>In recent years, an expansion of ambient pressure ionization mass spectrometry in various areas is observed [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0024"><span>24</span></a>,<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0025"><span>25</span></a>]. Atmospheric solids analysis probe mass spectrometry (ASAP-MS) is a powerful technique for direct analysis of even very complex samples, such as polymers [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0026"><span>26</span></a>], foods [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0027"><span>27</span></a>], or petrochemical sources and products [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0024"><span>24</span></a>]. ASAP ionization technique is a modification of atmospheric pressure chemical modification (APCI). In conventional arrangement, the sample is attached to a sampling probe tip (a standard melting point capillary is inserted into powder or liquid sample that sticks to its surface) and the probe is consequently placed into the ion source (commonly adapted ESI or APCI source). The analytes are released into the gas phase using hot gas (nitrogen) and ionized in the corona discharge. The formed ions (quasimolecular ions, radical cations or radical anions) are then transferred to the evacuated part of mass spectrometer [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0024"><span>24</span></a>,<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0025"><span>25</span></a>,<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0028"><span>28</span></a>]. Note that especially at higher vaporization temperatures a portion of the analytes is pyrolyzed and a mixture of parent ions and pyrolysis product ions is commonly observed in the ASAP-MS spectra [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0029"><span>29</span></a>]. The main feature of this direct MS mode is a fast detection of the formed products (including those with a higher mass and perhaps a shorter lifetime). In contrast, due to chromatographic separation in py-GC/MS, only relatively stable and volatile products are detected (the chromatographic step provides an additional selectivity here). In addition, electron impact ionization produces significantly different spectra profiles compared to the atmospheric pressure chemical ionization ongoing in the ASAP source. Both techniques are to a certain extent complementary [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0024"><span>24</span></a>,<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0025"><span>25</span></a>,<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0030"><span>30</span></a>].</p><p>There is a direct possibility of coupling mass spectrometry with ion mobility spectrometry (IMS) to significantly improve the selectivity of analysis (and, to certain extent, to compensate the advantage of the chromatographic step in py-GC/MS while maintaining the direct MS analysis speed). During an ion mobility spectrometry experiment, ions of analytes are flying through the ion mobility cell (e.g., a drift tube or a closed-loop path within a traveling wave ion guide in case of the cyclic ion mobility cell) filled with buffer gas (e.g. helium). In this process, ions collide with the buffer gas. These collisions slow down the ions differently based on their size, shape and charge. Ion mobility spectrometry therefore allows separation of the present analytes in the gas phase according to their collision cross sections (CCS). It also allows separation of isomeric compounds and filtration of the matrix signals, resulting in a higher quality of the MS and MS/MS spectra [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0031"><span>[31]</span></a>, <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0032"><span>[32]</span></a>, <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0033"><span>[33]</span></a>].</p><p>ASAP-MS has a high potential for the study of protective and decorative layers on the surfaces of historical objects. To the best of our knowledge, however, ASAP-MS and ASAP-IMS-MS have never been used for characterization of lacquers. This paper deals with the development of ASAP-MS method for characterization of Asian lacquers and its application for classification of oriental historical objects.</p><h4><strong>3. Material and methods</strong></h4><h5><strong>3.3. Instrumentation and experimental setup</strong></h5><p>Analyses were performed using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/202"><strong>Waters Cyclic IMS high resolution tandem mass spectrometer</strong></a> with an integrated cyclic ion mobility cell and atmospheric solids analysis probe (ASAP) used as ion source (Waters, Milford, MA, USA). Samples (∼ 0.1–0.5 mg) were inserted into a melting point capillary (G119/32, manufacturer SAMCO, United Kingdom) through an in-lab radially drilled hole (hole diameter: approx. 1 mm; distance from the bottom end approximately 5 mm). Solution of 2′,4′,6′-trihydroxyacetophenone monohydrate (THAP) in acetone was used as a standard for lock mass correction (<i>c</i> = 1 mg.ml<sup>-1</sup>). The m/z values were calculated as the means from three repeated measurements of appropriate samples. Approx. 1 μl of standard was deposited onto the capillary using a small pipette tip. Following experimental parameters were used: desolvation temperature of 600 °C, source temperature of 100 °C, corona “current” mode (corona current 2.0 μA), cone voltage of 30 V, source offset of 10 V, cone gas flow of 30 l/min, desolvation gas flow of 500 l/min, nebulizer gas pressure of 6 bar and reference capillary voltage of 2.5 kV. As for the ion mobility separation setup, a cyclic ion mobility cell was used in the following sequence: 1. Inject (10 ms), 2. Separate (37 ms), 3. Inject to Pre-Store (0.50 ms), 4. Eject (0.50 ms), 5. Reinject from Pre-Store (0.63 ms), 6. Eject and Acquire (13.20 ms). TW static height was 11 V, TW ramp start height was 15 V and TW ramp end height was 35 V. Acquisition time was 3 min for the MS scan and 1 min for the MS/MS scan used for identification of analytes. In the MS/MS mode, collision energy 30 V was used for pentadecyl and heptadecylcatechols, 35 V for phenols substituted with (CH<sub>2</sub>)<sub>10</sub>C<sub>6</sub>H<sub>5</sub> and 40 V for catechols substituted with (CH<sub>2</sub>)<sub>12</sub>C<sub>6</sub>H<sub>5</sub> and phenols substituted with CH<sub>2</sub>CO(CH<sub>2</sub>)<sub>10</sub>C<sub>6</sub>H<sub>5</sub>, respectively. Scan time 1 s was used for both scans. At the end of each working day, sample V was measured for confirmation that the instrument measured consistently. Values of m/z were calculated by MassLynx software (version 4.2, SCN 1016) after lock mass correction using the signal of protonated molecule of THAP. Theoretical mass of particular analyte was used for lock mass correction in the MS/MS spectra. Centroid top at 80 % and areas of peaks were used for accurate mass and intensity of signals calculation. The maximal accepted difference from theoretical mass (dtm) of signals was 10 mDa. Peak areas were used to compare the abundance of identified analytes among particular samples. Mobilograms from cyclic ion mobility experiments were produced by DriftScope v2.9 software. In mobilograms, spectra were generated by integration at Full Width at Half Maximum (FWHM) of peaks at drift time 1.72 ms.</p><h4><strong>4. Results and discussion</strong></h4><h5><strong>4.1. Utilization of ASAP-MS</strong></h5><p>In the ASAP-MS spectra of the studied lacquers, signals of a several compounds were observed (<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#fig0002"><span>Fig. 2</span></a>). Signals of radical cations as well as protonated molecules of identified analytes were easily visible. Usually a slightly higher abundance of cation radicals compared to protonated molecules was observed (spectra of all samples are listed in Supplementary material B). The processes observed in the ASAP-MS spectra involve the release of alkyl phenol/alkyl catechol monomer(s) and cleavages of alkyl chain(s) after the carbon linked to the phenol/catechol ring (i.e. cleavage after the first, second and third alkyl carbons) and elimination of water either from polymer or released monomers (the pyrolytic/collision-induced fragmentation processes are discussed below in detail). The analysis of provided samples revealed the most important markers of specific types of lacquers.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Journal_of_Cultural_Heritage_77_2026_187_196_Fig_2_Spectra_of_lacquers_prepared_from_Toxicodendron_vernicifluum_Toxicodendron_succedaneum_and_Gluta_usitata_samples_U_V_and_B_0200c24c06.jpg" alt="Journal of Cultural Heritage, 77, 2026, 187-196: Fig. 2. Spectra of lacquers prepared from Toxicodendron vernicifluum, Toxicodendron succedaneum and Gluta usitata (samples U, V and B)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Journal_of_Cultural_Heritage_77_2026_187_196_Fig_2_Spectra_of_lacquers_prepared_from_Toxicodendron_vernicifluum_Toxicodendron_succedaneum_and_Gluta_usitata_samples_U_V_and_B_0200c24c06.jpg 245w," sizes="100vw" width="2750" height="1269"></p><h5><strong>4.1.4. Study of MS/MS spectra of characteristic markers</strong></h5><p>For all the standard materials, the most important markers were subjected to fragmentation after the collision induced dissociation (CID) in the Trap collision cell of the mass spectrometer (MS/MS experiments; all fragmentation spectra and observed diagnostic fragments including differences from theoretical masses are given in Supplementary material B).</p><p>Signals of fragments with an intensity higher than the specified percentage of well known fragments arising from studied compounds were investigated and identified. Diagnostic fragments are given in Table B.2, Supplementary material B. The threshold signal was set for different compounds. For heptadecyl- and pentadecylcatechols, the threshold signal was defined as 5 % of the signal of dihydroxytropylium that is always an integral component of their collision spectra. For heptadecenyl- and pentadecenylcatechols the threshold signal was 2.5 % of dihydroxytropylium. For pentadecatrienyl catechol it was 100 % of dihydroxytropylium while for dodecylphenylcatechol it was 1 % of dihydroxytropylium. In case of 1-(3-hydroxyphenyl)-12-phenyldodecan-2-one, the threshold signal was defined as 17 % of the signal of hydroxyphenylethan-2-one. Finally, for 10-decylphenylphenol and 12-dodecylphenylphenol, 50 % of the signal of methylphenol was used as the threshold signal. These fragments were used for confirmation of the presence of lacquer compounds in routine measurements (at least one MS/MS spectrum was measured in each sample). The threshold signals were suggested and set in order to reach a sufficient yield of diagnostic fragments for unambiguous identification of individual markers. These signals are intended to be used in long term measurement in practice.</p><div><p>Fragmentation of heptadecylcatechols with 0-3 double bonds (<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#fig0003"><span>Fig. 3</span></a>a-d) is similar to that of pentadecylcatechols with same number of double bonds. In all cases, the signals of the characteristic fragments are well separated from the signals of the matrix.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Journal_of_Cultural_Heritage_77_2026_187_196_Fig_3_Fragmentation_spectra_of_heptadecylcatechols_possessing_0_3_double_bonds_6149330bc3.jpg" alt="Journal of Cultural Heritage, 77, 2026, 187-196: Fig. 3. Fragmentation spectra of heptadecylcatechols possessing 0-3 double bonds, (MS/MS; m/z of parent ions are: a) 342.2559, b) 344.2715, c) 346.2872, d) 348.3028)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Journal_of_Cultural_Heritage_77_2026_187_196_Fig_3_Fragmentation_spectra_of_heptadecylcatechols_possessing_0_3_double_bonds_6149330bc3.jpg 232w," sizes="100vw" width="3000" height="2017"></p><h5><strong>4.2. Principal component analysis</strong></h5><p>Excellent separation of particular types of Asian lacquers in Score Plot (<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#fig0005"><span>Fig. 5</span></a>a-d) was obtained by Principal Component Analysis based on the benzenediol and phenol derivatives described above. The Score Plot contains a set of 20 samples obtained from the Náprstek Museum Prague as well as other samples (BBI, BBO, SBI, SBO, U, V and B) in which lacquer compounds were detected (<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#tbl0002"><span>Table 2</span></a>). Note that in three other samples from The Náprstek Museum Prague, the lacquer compounds were not found at all.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Journal_of_Cultural_Heritage_77_2026_187_196_Fig_5_Score_plot_and_bi_plot_from_PCA_analysis_of_lacquers_taken_from_historic_objects_df82e0592a.jpg" alt="Journal of Cultural Heritage, 77, 2026, 187-196: Fig. 5. Score plot and bi-plot from PCA analysis of lacquers taken from historic objects, a. whole Score PCA plot, b. zoom of the T. succedaneum area, c. zoom of the T. vernicifluum area, d. zoom of the G. usitata area, e. whole PCA bi-plot, f. zoom of the T. succedaneum area in the PCA bi-plot, g. zoom of the T. vernicifluum area in the PCA bi-plot, h. zoom of the G. usitata area in the PCA bi-plot." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Journal_of_Cultural_Heritage_77_2026_187_196_Fig_5_Score_plot_and_bi_plot_from_PCA_analysis_of_lacquers_taken_from_historic_objects_df82e0592a.jpg 156w," sizes="100vw" width="3000" height="2994"></p><p>The significance of suggested markers for the studied lacquers across the whole data matrix was confirmed. The effect of particular markers is reflected in related bi-plots (<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#fig0005"><span>Fig. 5</span></a>e-h). Qualitatively, the results are in accordance with the former study obtained by py-GC/MS [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0001"><span>1</span></a>]. Since both cation-radicals and protonated molecules are formed in ASAP source with significant yield of both ion forms, their sum is an input into the data matrix for each substance (e.g., for pentadecenyl catechol, where the input is the sum of intensities I<sub>(m/z</sub> <sub>=</sub> <sub>318)</sub>+ <i>I</i><sub>(m/z</sub> <sub>=</sub> <sub>319)</sub>), see PCA matrix in Supplementary material C. Measured m/z values of the analyzed compounds are in good agreement with the theoretical values calculated for specific elemental compositions (noted differences from the theoretical mass, dtm is in all cases less than or equal to 10 mDa).</p><h4><strong>5. Conclusion</strong></h4><p>The combination of atmospheric solids analysis probe with high resolution tandem mass spectrometry proved to be a fast and effective tool for analysis of historical Asian lacquer objects, their resolution and classification. The developed method is based on profiling of phenol and benezenediols derivatives. The fragmentation of alkylphenols and alkylbenzene diols after collision activated dissociation in Trap and Transfer collision cells was described. Scission of alkyl chains and elimination of water represent the main fragmentation processes important for identification. Cyclic ion mobility separation appeared to be a useful tool for “cleaning” of signals of the lacquer ions from the matrix signals allowing their better identification from the fragmentation spectra. Moreover, ASAP-MS and MS/MS allowed detection of diterpenic resins, triterpenic resins and arsenic sulphide pigments present along with Asian lacquers. Principal Component Analysis provided complete separation of samples into three clusters of Asian lacquers in Score Plot. A simple classification approach based on direct comparison of newly suggested classification parameters (CPs) allows reliable differentiation of lacquer samples according to their provenance. The developed method is now implemented in routine controls of lacquered objects and could be applied to the analysis of resins and ambers obtained from archaeological findings.</p></div></div>

Characterization of Asian lacquers by atmospheric solids analysis probe high resolution tandem mass spectrometry coupled with cyclic ion mobility separation

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