This leaflet is intended to help participants in quantitative proficiency testing (PT) schemes to better understand the performance assessment made by the PT provider [1-4].<h3>Performance assessment parameters&nbsp;</h3><h4>Assigned value&nbsp;</h4>In order to assess individual performances, the results reported by participants are compared to the value assigned by the PT provider (xpt). ISO 13528 [1] suggests five different ways to obtain xpt (Figure 1). In this context, the design of the PT scheme must reflect the objectives of the scheme, comparing results either to a predetermined xpt (independent from the participant results), or to a value derived from the reported results. The PT provider is responsible for defining xpt , taking into account the advantages and disadvantages of the various possibilities when establishing metrological traceability or estimating the uncertainty of the assigned value, u(xpt).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Figure_1_e5ef5d1f9c.jpg" alt="Eurachem - Figure 1 Options for obtaining the assigned value, Xpt" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Figure_1_e5ef5d1f9c.jpg 245w," sizes="100vw" width="640" height="382"><h4>Uncertainty of the assigned value&nbsp;</h4>The uncertainty of the assigned value u(xpt) can be estimated in at least five different ways [1], each corresponding to the value assignment strategy illustrated in Figure 1. The different uncertainty evaluation methods are listed in Figure 2. This uncertainty can be reported as a standard uncertainty (u (xpt)), or as an expanded uncertainty U (xpt) with a coverage factor k, where U (xpt) = k . u (xpt). The choice of k will impact the level of confidence (e.g. approximately 95 % for k = 2).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Figure_2_f41ebc0c61.jpg" alt="Eurachem: Figure 2 Options for obtaining the uncertainity of the assigned value, u(Xpt)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Figure_2_f41ebc0c61.jpg 245w," sizes="100vw" width="727" height="378"><h4>Standard deviation for proficiency assessment&nbsp;</h4>Five possibilities are suggested by ISO 13528 to set the standard deviation for proficiency assessment spt (Figure 3). The chosen approach must comply with the objectives of the PT scheme.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Figure_3_f09cb3969e.jpg" alt="Eurachem: Figure 3 Options for obtaining the standard deviation for proficiency assesment" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Figure_3_f09cb3969e.jpg 245w," sizes="100vw" width="638" height="355">Two approaches for performance assessments use spt as shown in Table 1.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Table_1_Scoring_for_performance_assessment_3dd03aa3fb.jpg" alt="Eurachem: Table 1 - Scoring for performance assessment." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Table_1_Scoring_for_performance_assessment_3dd03aa3fb.jpg 245w," sizes="100vw" width="1390" height="571"><h3>Performance assessment&nbsp;</h3>All performance assessments are based on the difference between the participant’s results (xi) and the assigned value (xpt) divided by a normalisation factor (Table 1).&nbsp;<ul><li>The percent difference D% is the normalised difference expressed as a percentage of xpt , for which a prescribed maximum relative permissible error (dE% = dE / xpt) is defined by the PT provider.&nbsp;</li><li>The z score (unitless) compares the participant’s deviation from the assigned value with the standard deviation for proficiency assessment (spt). When the uncertainty of the assigned value is significant (u(xpt) &gt; 0.3 spt), the z’ score should be used instead, to include the contribution of u(xpt) in the denominator.&nbsp;</li><li>The zeta (ζ) score (unitless) states whether the laboratory’s result agrees with the assigned value within the respective uncertainty. The denominator is the combined (standard) uncertainty of the assigned value u(xpt) and the (standard) measurement uncertainty reported by the laboratory u(xi). An unsatisfactory zeta score can either be caused by an inappropriate estimation of the measured quantity value (e.g. concentration, content), or of its measurement uncertainty, or both.&nbsp;</li><li>The En score (unitless), unlike the zeta score, uses a combination of expanded uncertainties as the normalisation factor. This combination corresponds to a confidence interval of approximately 95 %. En scores are often used in metrology comparisons among calibration laboratories.</li></ul><h3>Interpretation of performance scores&nbsp;</h3>According to ISO 13528:2022 clause 9.4.2, the conventional interpretation of the z, z’ and ζ scores is as follows:&nbsp;<ul><li>A result that gives |score| ≤ 2.0 is considered to be acceptable, indicating satisfactory performance.&nbsp;</li><li>A result that gives 2.0 &lt; |score| &lt; 3.0 is considered to give a warning signal, indicating questionable performance.&nbsp;</li><li>A result that gives |score| ≥ 3.0 is considered to be unacceptable (or action signal), indicating unsatisfactory performance.&nbsp;</li></ul>Similarly, |En| &lt; 1 and |D%| &lt; dE% indicate successful (acceptable) performance.

Understanding PT performance assessment

The NIST 26 software platform integrates deconvolution and library searching for both EI GC-MS and LC-MS/MS workflows. One of its most useful features is the ability to send spectra directly from the Chromatogram Window to the Lib Search module, allowing analysts to perform more comprehensive library searches and review a broader range of candidate compounds.This guide summarizes the key concepts presented by James Little in his tutorial on performing Normal EI library searches using spectra exported from the Chromatogram Window. It focuses on the practical workflow, search options, result interpretation, and best practices for obtaining reliable compound identifications.<h4>Why Send Spectra from the Chromatogram Window to Lib Search?</h4>When an EI Normal Identity search is performed directly within the Chromatogram Window, only the highest-ranked identification is displayed for each unknown component. While this approach is useful for rapid screening, it does not provide insight into alternative candidate compounds.Sending the spectrum to Lib Search offers several advantages:<ul><li>Displays a large list of potential matches instead of only the top hit.</li><li>Provides additional information that may help confirm or challenge the primary identification.</li><li>Allows comparison of multiple candidate compounds.</li><li>Supports more detailed investigation of unknown peaks.</li><li>Enables the use of additional search methods and library resources.</li></ul>This is particularly valuable when the top identification is uncertain or when multiple compounds produce similar mass spectra.<h4>Sending a Spectrum to Lib Search</h4>To examine a chromatographic peak in greater detail:<ol><li>Locate the component of interest in the Chromatogram Window.</li><li>Right-click on the selected component.</li><li>Choose Library Search from the context menu.</li><li>The selected spectrum is transferred directly into Lib Search for further evaluation.</li></ol>Once transferred, the analyst gains access to a much larger list of candidate compounds than is available within the Chromatogram Window alone.<h4>Configuring Search Settings</h4>Before reviewing search results, it is important to verify that the search configuration is appropriate.<h5>Identity Search Mode</h5>Select:<ul><li>Identity</li><li>EI Normal</li></ul>This is the same search mode used by the Chromatogram Window and is typically the best starting point for unknown identification.<h5>Full Spectrum Search</h5>For most applications, a Full Spectrum Search should be used.This approach compares the complete unknown spectrum against library reference spectra and generally provides the most reliable results when spectra are relatively clean and free from significant interference.<h5>Library Selection</h5>NIST 26 allows searching across multiple libraries simultaneously.Possible library sources include:<ul><li>Main NIST library</li><li>Replicate library</li><li>Wiley libraries</li><li>User-created libraries</li><li>Specialized commercial libraries</li></ul>Results from all selected libraries are combined into a single candidate list.<h5>Save Configuration</h5>Frequently used search settings can be saved for future use, including:<ul><li>Search options</li><li>Library selections</li><li>Window layouts</li><li>Fonts</li><li>Display settings</li></ul>Saving configurations helps maintain consistency across projects and analysts.<h4>Understanding the Lib Search Interface</h4>Several key interface elements help analysts interpret results efficiently.<h5>Number of Candidate Spectra</h5>The top status bar displays the total number of candidate spectra returned by the search.Large candidate lists are common when searching compounds that have multiple reference entries in the libraries.<h5>Search Type Indicator</h5>The bottom-right status area shows:<ul><li>Search type performed</li><li>Spectrum display mode</li></ul>This information is useful for confirming that the intended search method was applied.<h5>Butterfly Plot Comparison</h5>One of the most useful visualization tools is the butterfly plot.In this display:<ul><li>The unknown spectrum is shown on the top.</li><li>The selected library spectrum is shown on the bottom.</li></ul>This format allows rapid visual comparison of spectral similarities and differences, helping analysts assess identification confidence.<h4>Reducing Duplicate Search Results</h4>Many compounds appear multiple times within large spectral libraries.As a result, the initial search may return numerous duplicate entries for the same compound.<h5>Best Matching Only Filter</h5>The Best Matching Only option helps simplify result review by:<ul><li>Removing most duplicate entries.</li><li>Reducing clutter in the hit list.</li><li>Making candidate evaluation faster and easier.</li></ul>Importantly, this filter is applied after the presearch step and can be switched on or off at any time without repeating the search.<h4>Choosing the Right Search Method</h4>NIST 26 offers several search strategies beyond the standard Full Spectrum Search.<h5>Full Spectrum Search (Score)</h5>This method compares the entire unknown spectrum against library entries.Advantages:<ul><li>Excellent for clean spectra.</li><li>Produces reliable overall similarity scores.</li><li>Suitable for routine unknown identification.</li></ul>Best used when spectral interference is minimal.<h5>Reverse Search (Impurity Tolerant Search, Rev-Dot)</h5>Reverse searching evaluates whether the important peaks present in the library spectrum are found in the unknown spectrum.Unlike Full Spectrum Search:<ul><li>Extra peaks in the unknown spectrum are not heavily penalized.</li><li>More tolerant of contamination and coelution.</li><li>Better suited for noisy or mixed spectra.</li></ul>Because of these characteristics, Reverse Search is often preferred when dealing with real-world chromatographic data.<h4>Why Reverse Search Works Well with Wiley Libraries</h4>The Wiley libraries contain numerous spectra derived from published literature sources.Many of these entries are partial spectra rather than complete reference spectra.For this reason:<ul><li>Reverse Search frequently performs better than Full Spectrum Search.</li><li>Partial reference spectra can still produce high-quality matches.</li><li>Additional compounds may be identified that would otherwise be overlooked.</li></ul>When using Wiley databases, Reverse Search is often recommended as a complementary identification strategy.<h4>Understanding the Role of Presearch</h4>NIST uses a presearch stage to accelerate library searching.The process works by:<ol><li>Rapidly identifying promising candidates.</li><li>Creating a reduced candidate list.</li><li>Performing a more detailed comparison on those candidates.</li></ol>This dramatically increases search speed while maintaining identification quality.However, one important limitation exists:If Reverse Search ranking is applied only after a Full Spectrum presearch, potentially useful candidates may have already been removed during presearch filtering.Therefore, when Reverse Search is desired, it is often best to specify the Reverse Search method before the search begins.<h4>Sorting Search Results</h4>Search results can be organized using different scoring metrics.<h5>Match Score</h5><ul><li>The default ranking method is the Match Score.</li><li>This remains the preferred metric for most clean-spectrum identifications.</li></ul><h5>Reverse Match Score</h5>Results can also be sorted according to Reverse Match.Benefits include:<ul><li>Improved evaluation of impure spectra.</li><li>Better handling of coeluting compounds.</li><li>Enhanced performance with partial library spectra.</li></ul>For optimal Reverse Match results, the search should be performed using the Impurity Tolerant Search (Rev-Dot) method from the beginning rather than simply re-sorting the final hit list.<h4>Partial Spectrum Search (PSS-Dot)</h4>NIST also includes a Partial Spectrum Search option.This method is primarily intended for:<ul><li>Tandem MS applications</li><li>Targeted spectral matching</li><li>Situations where only part of the spectrum is available</li></ul>For standard EI unknown identification, Full Spectrum Search and Reverse Search are generally preferred.<h4>Best Practices</h4>To obtain the most reliable EI library search results:<ul><li>Begin with a standard EI Normal Full Spectrum Search.</li><li>Review multiple candidate hits rather than relying solely on the top identification.</li><li>Use the Best Matching Only filter to simplify interpretation.</li><li>Apply Reverse Search when spectra contain impurities or coeluting compounds.</li><li>Consider Reverse Search when using Wiley library entries.</li><li>Configure search methods before the search begins whenever possible.</li><li>Use butterfly plots to visually verify spectral agreement.</li><li>Save preferred configurations for future projects.</li></ul><h4>Conclusion</h4>The Lib Search module in NIST 26 significantly expands the analytical capabilities available from the Chromatogram Window. While the Chromatogram Window provides rapid identification using the top-ranked candidate, Lib Search enables a much deeper examination of unknown compounds by presenting extensive candidate lists, advanced search methods, and powerful visualization tools.Understanding when to use Full Spectrum Search, Reverse Search, and filtering options such as Best Matching Only allows analysts to improve confidence in compound identification, particularly when dealing with complex, noisy, or partially resolved chromatographic data. By combining these tools with multiple spectral libraries, users can maximize the value of NIST 26 for routine EI GC-MS identification workflows.

Normal EI Search for Spectra Sent from Chromatogram Window

ISC 2026 Program

Article

Events

Academy

Video

Software

Eurachem is a European network promoting international traceability of chemical measurements and good analytical practice. It provides expert guidance, supports method validation, uncertainty evaluation, proficiency testing, and facilitates discussion on technical and policy issues in analytical quality.

Eurachem

The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

My main interest is the identification of organic compounds by mass spectrometry in organic mixtures.

James Little/Mass Spec Interpretation Services

Separation Science

Mettler-Toledo

Agilent Technologies

AZoM

LECO

Thermo Fisher Scientific

Restek Corporation

Waters Corporation

<div><h4>Navigating Challenges and Opportunities for Environmental Testing Labs Under the Revised EU Water Framework Directive</h4>The European Commission’s proposed updates to the Water Framework, Groundwater, and Environmental Quality Standards Directives are bringing new responsibilities and opportunities for environmental testing labs. With expanded pollutant lists - including PFAS, pharmaceuticals, and pesticide metabolites - and stricter monitoring and reporting requirements, the landscape of water analysis is rapidly evolving.Join the 4th Waters Environmental Webinar Series to explore how your lab can navigate these challenges and turn regulatory change into opportunity.Register for free and partner with us to shape the future of European water quality.<h5>Why Attend</h5>Stay ahead of new EU regulatory demands with insights from leading experts. Gain practical guidance, share experiences with peers, and prepare your lab for the next generation of water quality standards.<h5>Highlights</h5><ul><li>New pollutant categories and analytical challenges</li><li>Updates to monitoring protocols and reporting</li><li>Innovative methods to meet revised Environmental Quality Standards</li><li>Strategic guidance for compliance and adaptation</li></ul>Lead the change in water analysis—stay ahead, stay compliant, and make a real impact.<h4>EPISODE 6: Blue Thursdays 2026 – The Best Of</h4>In this final session, we’ll look back at the most important things we’ve learned throughout the Blue Thursday series. We’ll explore the big trends we’re seeing, the smart tools scientists use to study the environment, and what exciting developments will be coming next.<blockquote>Speaker: Claudia Rathmann (Principal F&amp;E Market Development Manager, Waters Corporation)Experienced and passionate analytical science professional with over 20 years of technical and strategic expertise in food and environmental applications. Currently serving as Principal Market Development Manager at Waters Corporation, Claudia specializes in connecting customers with product management to deliver innovative solutions for complex analytical challenges. With a strong scientific foundation and a proven track record in both technical and marketing roles, Claudia bridges the gap between customer needs and product innovation across the EMEA region.Speaker: Janitha De-Alwis (JD) (Principal F&amp;E Market Development Manager, Waters Corporation)Janitha De-Alwis (known as JD) is an applications chemist in the European demo lab, based in Wilmslow, UK. His main role focuses on application development work for the food and environmental sector. JD takes great pride in speaking to, and advising, many food and environmental customers. His position requires him to be reactive and proactive to the changes in the food industry, such as changing regulations, topical issues and food safety risks.</blockquote></div>

Blue Thursdays 2026 – The Best Of

In this final session, we’ll look back at the most important things we’ve learned throughout the Blue Thursday series.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>High-performance plastics (polymers) are the backbone of modern aerospace, medical, analytical instrumentation, and energy technologies. Because they are designed to survive extreme heat and chemicals, they are notoriously difficult to break down and study. To build better materials and ensure they don’t fail, scientists need to understand their chemical composition including the additives and “building blocks” that give them their strength.This webinar demonstrates two specialized sample delivery techniques to characterize these materials without the need for elaborate sample preparation strategies:<ul><li>Thermal Desorption: Controlled heating (up to 300 °C) of the plastic to release additives and potential contaminants.</li><li>Pyrolysis: Using intense heat (greater than 600 °C) to break the plastic’s “backbone” into identifiable fragments.</li></ul>Combining these techniques with high-resolution GC-TOFMS enables detection of the complex chemical fingerprints for high-performance plastics like polyether ether ketone (PEEK, used in medical implants) and polytetrafluoroethylene (PTFE, commonly known as Teflon).We will demonstrate a reliable method to analyze the chemistry of the world’s toughest plastics. This method will help manufacturers ensure their materials are pure, and ready for use in planes, electronics, and human bodies.<blockquote>Presenter: David Alonso (Applications Chemist, LECO Corporation)</blockquote></div></div></div>

LECO: Characterization of High-Performance Polymers Using Thermal Desorption/Pyrolysis and High-Resolution Mass Spectrometry 

Characterization of High-Performance Polymers Using Thermal Desorption/Pyrolysis and High-Resolution Mass Spectrometry 

Advanced GC-TOFMS workflows using thermal desorption and pyrolysis for chemical characterization of high-performance plastics like PEEK and PTFE.

Polymer characterization by TD/Pyrolysis & HRMS

PFAS &amp; Our Planet is a webinar series on PFAS testing in environmental samples, food and food contact materials, textiles and other materials. Alongside the laboratory and regulatory lens, the series addresses the broader planetary impact of PFAS, including their persistence and movement through water, soil, air, food systems, and materials. It brings together what laboratories and industry need to understand now: where PFAS rules are heading, which sample types are under increasing focus, what needs to be measured, and how those needs translate into method selection, workflow setup, and result reporting.<h4>Webinar Series Details</h4><ul><li>One registration to attend the full three-part series</li><li>Questions for experts in advance and/or live Q&amp;A</li><li>Access to recorded sessions provided</li></ul><h4>Who Should Attend:&nbsp;</h4>Environmental and water testing laboratories, food testing laboratories, food contact material and packaging laboratories, textile and materials testing laboratories, contract testing laboratories, public sector and regulatory laboratories, manufacturers of food, packaging, textiles, and consumer goods, quality, product safety, regulatory affairs, and sustainability functions, and research laboratories working on PFAS analysis.<h4>Recommended for:&nbsp;</h4>Analytical chemists, laboratory managers, QA/QC managers, regulatory affairs managers, food safety and product safety managers, environmental scientists, packaging and food contact materials specialists, textile and materials testing leads, sustainability and product stewardship managers, validation and method development scientists, and technical leaders in contract, public sector, and research laboratories.<h4>Regulatory context covered:&nbsp;</h4>EU and U.S. PFAS developments, food contact and packaging measures, environmental monitoring frameworks, OEKO-TEX requirements for textiles and materials, Singapore’s food and water surveillance activity, India’s draft PFAS-related packaging amendment, and emerging movement in parts of Southeast Asia like Thailand, Malaysia, Vietnam, Indonesia, Bangladesh and Philippines.<h3>Textiles and Materials</h3>Textiles and treated materials are facing stronger PFAS scrutiny as product restrictions widen and supply chains are asked to verify material content more clearly. This session will cover the main external reference points shaping that shift, including the EU REACH PFAS restriction process and OEKO-TEX requirements, which have become highly relevant for textile and material suppliers serving global brands.It will then step into the laboratory workflow for textiles and related materials, covering extraction and cleanup, targeted LC/MS/MS for non-volatile PFAS, selected GC/MS/MS approaches for volatile PFAS where relevant, contamination control, and reporting formats suited to product verification. Regional context will be framed around export-market pull, buyer specifications, and certification-driven testing, which are often the main drivers for suppliers in South and Southeast Asia.<blockquote>Presenter: David Saladin (Ecology Lab Team Leader, TESTEX AG, Swiss Textile Testing Institute)Presenter:&nbsp;Aimei Zou, Ph.D. (Application Scientist, Agilent Technologies, Singapore)</blockquote>

Agilent Technologies: PFAS & Our Planet: Textiles and Materials

PFAS & Our Planet: Textiles and Materials

PFAS in textiles webinar: regulations, LC-MS/MS and GC-MS/MS workflows, contamination control, and compliance for global supply chains.

<h3>Real-Time Process Insights for Oil, Gas, &amp; Future Fuels with Raman Spectroscopy</h3><h4>A 5-Part Webinar Series</h4>As the energy sector shifts toward more complex and dynamic processes, real-time analytics are essential for maintaining control, efficiency, and product quality. This 5-part webinar series explores how process Raman spectroscopy and integrated measurement solutions enable fast, in-line analysis across oil &amp; gas, power generation, and emerging energy applications.<h5>What we'll cover:</h5>Each session highlights practical approaches to improving process visibility, reducing analytical delays, and supporting faster decision-making for:<ul><li>Natural gas</li><li>Refined fuels</li><li>Hydrogen</li><li>Sustainable aviation fuels</li><li>Carbon capture</li><li>Power generation</li><li>Emerging energy applications</li></ul><h5>This Series is Ideal For:</h5>Process, operations, and instrumentation professionals across oil &amp; gas, power generation, and emerging energy sectors, including engineers, plant and refinery managers, and technical specialists involved in process optimization and analytical measurement.<h5>Join the Webinars To:</h5><ul><li>Understand how real-time, in-line analytics improve process control and efficiency</li><li>Learn how process Raman simplifies and accelerates gas and liquid analysis</li><li>Explore alternatives to traditional methods such as gas chromatography</li><li>See how integrated measurement solutions enhance operational visibility</li><li>Gain insight into applications across conventional and next-generation energy systems</li></ul><h4>Webinar 5: Optimizing Future Fuel Processes with Process Raman Spectroscopy: Applications in Hydrogen, Sustainable Aviation Fuels &amp; Carbon Capture</h4>The global transition to future fuels, such as sustainable aviation fuel (SAF), hydrogen, and carbon capture, utilization and storage (CCUS), is reshaping how energy and chemicals are produced, monitored, and controlled. Real-time analytical technologies are becoming critical to ensure process efficiency, product quality, and regulatory compliance across these emerging applications. Process Raman spectroscopy enables in-line, non-destructive measurement of complex chemical systems with minimal sample preparation.By delivering rapid, data-rich measurements from a single scan, process Raman allows simultaneous quantification of multiple components in gases, liquids, and heterogeneous systems. This reduces reliance on slow, offline techniques and enables tighter process control and faster decision-making. The technology combines robust probe design, flexible sampling configurations, and seamless integration into industrial control systems. Its compact, field-deployable design allows deployment at multiple points from pilot to full-scale production.This webinar will discuss the application of process Raman spectroscopy in real-time monitoring of SAF production, hydrogen isomer conversations and CCUS applications.<blockquote>Speaker: Linxi Chen (Field Application Scientist, Thermo Fisher Scientific)</blockquote>

Thermo Fisher Scientific: Real-Time Process Insights for Oil, Gas, & Future Fuels with Raman Spectroscopy

Optimizing Future Fuel Processes with Process Raman Spectroscopy: Applications in Hydrogen, Sustainable Aviation Fuels & Carbon Capture

Join this webinar to discover how process Raman spectroscopy enables real-time analysis and process control for SAF, hydrogen, and CCUS applications.

Process Raman in Hydrogen, Sustainable Aviation Fuels

Discover how today’s advanced peptide technologies are shaping tomorrow’s research, development, and manufacturing success.<h5>In this symposium, you'll learn about:</h5><ul><li>Scalable peptide API manufacturing and purification expertise for GLP‑1 class molecules</li><li>Label‑free, real‑time cell‑based bioassays for peptide activity, optimization, and safety testing</li><li>Non‑destructive Raman raw material identification through opaque containers to streamline GMP workflows</li><li>Inert LC/MS strategies for enhanced peptide separation, impurity profiling, and molecular weight confirmation</li></ul>This digital symposium explores the innovations shaping the future of peptide‑driven science, featuring modern analytical tools across cell analysis, chemistries, spectroscopy, and mass spectrometry. Experts will reveal practical approaches for integrating these technologies to strengthen and streamline peptide analysis.Attendees will gain a clear view of emerging technologies and practical strategies across modern research and applied science. Join the symposium to discover how to connect these advances directly to your own work.10:00 - 10:05 - IntroVitan Blagotinsek, Agilent Technologies10:05 - 10:20 - Highlight Talk - Scaling the GLP-1 Surge: Peptide API Development, Purification and Manufacturing at AATWolfgang Schiek, BioVectraAs the paradigm shift in diabetes management and the surge in anti-obesity drug sales and development are propelling unprecedented demand for complex peptide APIs therapies, reliable high quality peptide API manufacturing and purification are critical. Agilent Advanced Therapeutics provides specialized expertise and scaling capacity for these GLP-1 Agonists. We support our customers from preclinical to commercial through swift, customer-focused implementation in state-of-the-art facilities and are continuously expanding capabilities, true to our commitment: Invested in Science, Invested in You!10:20 - 11:05 - Talk 1: Accelerating Synthetic Peptide Discovery, Quality Control, and Safety Testing with xCELLigence BioassayLeyna Zhao, Agilent TechnologiesSynthetic peptides and proteins are rapidly emerging as important therapeutic modalities, driving the need for robust approaches to evaluate biological activity, product quality, and safety throughout development. In this webinar, we will explore how Agilent’s label free xCELLigence Real Time Cell Analysis (RTCA) platforms can support synthetic peptide discovery, quality evaluation, and safety testing through functional, cell based bioassays. Selected case studies will highlight the use of real time, kinetic cellular readouts in peptide discovery and optimization workflows. Evaluation criteria for biological products will be reviewed in the context of ICH Q6B specifications, with emphasis on the role of bioassays in assessing biological activity. In addition, examples of toxicity testing using xCELLigence RTCA—including published vaccine related applications—will demonstrate how real time cell based assays can provide sensitive and reproducible QC results.11:10 - 11:50 - Talk 2: Unlocking Deeper Insights into Peptide Purity and Impurity Profiling with LC/MS for GLP-1 RA CharacterizationHolger Stalz, Agilent TechnologiesSynthetic Peptide therapeutics are reshaping the biopharma landscape, and GLP-1 receptor agonists are at the center of that momentum—raising the stakes for purity assessment and impurity profiling. Join this webinar to see how Agilent’s advanced LC/MS workflows help scientists tackle these challenges with greater confidence and efficiency. You’ll learn how the InfinityLab Pro iQ Plus LC/MSD enables reliable peptide purity confirmation, while the Agilent BioQTOF high-resolution LC/MS/MS system supports detailed sequence confirmation and impurity identification. We’ll also show how OpenLab CDS and MassHunter BioConfirm software simplify complex data interpretation, helping QC and R&amp;D teams move faster and make more confident decisions in peptide characterization.11:55 - 12:35 - Talk 3: Raman Spectroscopy in Peptide PharmaceuticalsJan Wuelken, Agilent TechnologiesRapid, non destructive raw material identification (RMID) through all types of packaging. Reliable identity verification of peptides, amino acids, protected building blocks, and excipients is a critical step in peptide manufacturing. The Agilent Vaya Raman Raw Material Identity Verification System is specifically designed to identify raw materials directly through non transparent and opaque containers, without opening the package. By leveraging Spatially Offset Raman Spectroscopy (SORS), Vaya minimizes interference from container walls, liners, and fluorescence. This enables fast and reliable identification through typical peptide pharma packaging, including plastic drums, paper and multi layer bags, FIBCs, and amber glass containers. The through container measurement capability eliminates the need for sampling, significantly reducing the risk of cross contamination and protecting operators from hazardous, highly potent, or sterile materials. At the same time, it streamlines workflows by reducing handling steps and operator exposure. Agilent Vaya supports high throughput, GMP compliant raw material testing, accelerates release to manufacturing, and helps ensure a secure, efficient supply of materials for peptide synthesis and downstream processing—from early development to commercial production.12:40 - 13:15 - Talk 4: Advanced Column Solutions to enhance your Peptide Analysis and Purification WorkflowsKatrien Sabbe, Agilent TechnologiesWhether you're optimizing existing methods or developing new ones, this session will equip you with the tools to streamline your peptide characterization and purification. LC analysis of peptides can be challenging due to their sensitivity to metal interactions within chromatographic systems, often leading to poor peak shape and reduced sensitivity. Agilent Altura HPLC columns with Ultra Inert technology overcome these challenges by minimizing metal-analyte interactions. We will illustrate how this innovation enhances resolution, reproducibility, and quantification in reversed phase and HILIC separations of therapeutic peptides such as GLP-1 receptor agonists. Furthermore we will explore preparative techniques and purification workflows, offering tips for achieving high purity and recovery in synthetic peptide purification.<blockquote>Presenter: Vitan Blagotinsek (EMEA Marketing Program Manager Bio/Pharma, Agilent Technologies)Vitan is the Agilent Marketing Manager for the Pharma and Biopharma markets across the EMEA region, covering a broad portfolio of all Agilent products with relevance to these markets. Prior to this role, he had started at Agilent as a Chromatography and Sample prep-focused Product Specialist targeting the Biopharma market in the DACH region. His scientific background is in Protein Biochemistry/Structural Biology. He graduated in Biochemistry from the University of Oxford in the UK and has a PhD from Marburg University in Germany (Department of Chemistry).</blockquote><blockquote>Presenter:&nbsp;Wolfgang Schiek (Director Business Development, BIOVECTRA, Agilent Technologies)Wolfgang is the BD Director Europe for Agilent Advanced Therapeutics, where he specializes in bespoke CDMO solutions and a chemist by profession. With a 25-year track record at industry leaders like Evonik, Cambrex, and Novasep in a variety of technical, commercial and strategic roles, he has helped numerous clients bridge the gap from drug development to successful market launch. For the past four years at AAT, he has focused on the cutting edge of biologics and peptides, serving as one of the primary forces behind the company’s GLP-1 programs.</blockquote><blockquote>Presenter: Leyna Zhao (Product Marketing Director, Agilent Technologies)Leyna is Product Marketing Director at Agilent Technologies, where she leads global strategy for cell analysis platforms, including xCELLigence Real-Time Cell Analysis (RTCA). With over 20 years of experience in molecular biology, immunology, and drug discovery, she bridges scientific innovation with market adoption. Her work focuses on advancing label-free, real-time technologies that support small molecule screening, immune cell function analysis, and predictive toxicology. She holds a PhD in Biochemistry and has been instrumental in aligning Agilent’s cell analysis solutions with emerging regulatory trends, such as non-animal testing methodologies.</blockquote><blockquote>Presenter: Jan Wuelfken (Product Specialist, Molecular Spectroscopy EMEA IDO, Agilent Technologies)Jan graduated from the University of Hamburg in 1997 with a thesis about NMR-based conformation analysis of an antimicrobial peptide and in 2001 with his PhD thesis about CD4-binding peptides as inhibitors for HIV infection. He joined Varian Inc. in 2001 as a sale representative for molecular spectroscopy and with the takeover of Varian by Agilent 2010 he became a product specialist for molecular spectroscopy in the Agilent International Distributor Organization (IDO). Today his responsibility is the full Agilent Molecular Spectroscopy Portfolio which is UV-Vis, UV-Vis/NIR, Fluorescence, FT-IR and Raman Spectroscopy.</blockquote><blockquote>Presenter:&nbsp;Holger Stalz (LCMS Product Specialist, Agilent Technologies )Holger has worked as a product specialist for LC/MS for Agilent in Austria and Switzerland since 2011. He leads a team of European BioPharma Specialists focusing on biopharmaceutical applications and has been coordinating the development of new analytical solutions and methods for protein therapeutics and oligonucleotide-based products.</blockquote><blockquote>Presenter:&nbsp;Katrien Sabbe (Biopharma Business Development Manager, Agilent Technologies)For more then 25 years Katrien has been working in the field of Chromatography Columns and Supplies, first at VWR International and later at Varian Analytical Instruments, which was acquired in 2011 by Agilent Technologies. Currently, she is the EMEA Biopharma Business Development Manager at Agilent Technologies Chemistries and Supplies Division.</blockquote>

BiteSizeBio: Innovations and Emerging Tools in Peptide Science

Innovations and Emerging Tools in Peptide Science

Explore advanced peptide technologies. Learn about GLP-1 API manufacturing, xCELLigence bioassays, through-container Raman RMID, and inert LC/MS workflows

Advanced Peptide Technologies and GLP-1 Analysis

Today’s demanding GC and GC/MS applications place increasing pressure on laboratory productivity and operating costs. While advancements in GC column inertness over the past decade have enhanced data quality, improved inertness alone is no longer sufficient for laboratories striving to remain competitive. Agilent’s premium 5Q columns build on Ultra Inert performance with added premium GC column features including enhanced thermal stability, ultra‑fast conditioning, and extended column lifetime. Join this webinar to see how these premium features can elevate your analytical performance and drive meaningful improvements in productivity.<blockquote>Speaker: Ashlee Gerardi (Product Marketing, Agilent Technologies, Inc.)Ashlee Gerardi is a GC Columns Product Manager at Agilent Technologies. After receiving her B.S. in Chemistry, Ashlee began her career as an R&amp;D chemist, where she developed novel stationary phases and surface deactivations for GC columns. Ashlee then received her MBA and transitioned to business development overseeing the cannabis and hemp markets. She then served as a market manager for the chemicals, semiconductor, and mining segments.Within her current role at Agilent, Ashlee is currently working on commercializing new GC columns and workflows.&nbsp;</blockquote>

Agilent Technologies: Becoming a Better Chromatographer - Webinar Series

Becoming A Better Chromatographer: Understanding the Impact of Advanced GC Column Features for Improving Productivity and Data Quality in GC/MS Workflows

Improve GC/MS workflows with Agilent 5Q columns. Benefit from enhanced thermal stability, ultra-fast conditioning, and ultra-inert peak shapes

Agilent 5Q Columns for Advanced GC/MS Workflows

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>Ready to modernize your dioxin analysis? Join this webinar to learn about the Orbitrap Exploris™ GC S mass spectrometer and its application in dioxin analysis for food control laboratories. The session will outline how high-resolution GC-MS with Orbitrap technology can deliver the analytical performance required for regulatory compliance. It will also consider how such approaches support the analysis of dioxins and other persistent organic pollutants in routine workflows. The presenters - Dr Kerstin Krätschmer &nbsp; (Wageningen Food Safety Research) and Dominic Roberts (Thermo Fisher Scientific), will present application data, review analytical performance against regulatory criteria, and discuss current challenges in dioxin analysis, including considerations when evaluating alternatives to traditional magnetic sector GC-HRMS systems.<h4>Learning points</h4><ul><li>Learn about the new Orbitrap Exploris GC S released at ASMS 2026.</li><li>See how high-resolution GC-MS meets dioxin regulatory requirements and expands analytical capability.</li><li>Learn how to modernize workflows for simpler, future-ready dioxin &amp; POPs analysis.</li></ul><h4>Who should attend?</h4><ul><li>Laboratory managers, supervisors, and analysts working in food safety and environmental labs.</li><li>Analysts performing dioxin and POPs analysis interested in learning about latest technology and application approach.</li></ul><blockquote>Presenter: Dr Kerstin Krätschmer (Head of the Dutch National Reference Laboratory for POPs, Wageningen Food Safety Research (WFSR))Kerstin has a PhD in food chemistry and is currently head of the Dutch NRL POPs situated at Wageningen Food Safety Research (WFSR) in Wageningen, the Netherlands. She earned her PhD at the European Union Reference Laboratory for halogenated POPs in Freiburg, Germany, exploring polychlorinated alkanes analysis using GC-Orbitrap-HRAM. Within her current work as researcher in the contaminants team at WFSR, she covers a wide range of topics from PCDD/Fs to radioactivity in food, looking into new detection methods, fate in the circular food system, emerging risks and crisis preparedness.Presenter:&nbsp;Dominic Roberts (Product Marketing Manager, GC Orbitrap MS, Thermo Fisher Scientific)Dominic Roberts has been closely involved with the Thermo Scientific GC Orbitrap MS since its launch in 2015. He started at Thermo Fisher Scientific as a Senior Applications Specialist for the product and is currently the GC Orbitrap MS Product Marketing Manager. Dominic is an experienced analytical chemist and mass spectrometrist with more than 25 years of experience using state-of-the-art analytical systems. He has worked in both a regulatory and industrial environment with GC–MS and LC–MS systems to investigate a variety of chemicals in food, biological, and environmental samples.</blockquote></div></div></div>

Thermo Fisher Scientific: Modernizing Dioxin Analysis for Food Control: Introducing a New GC Orbitrap

Modernizing Dioxin Analysis for Food Control: Introducing a New GC Orbitrap

Join webinar to learn about Orbitrap Exploris GC S mass spectrometer and its application in dioxin analysis for food control laboratories.

Dioxin Analysis with New GC Orbitrap

<header>The ongoing urbanization and progressive sealing of landscapes have exacerbated surface runoff, mobilizing pollutants such as Total Suspended Solids (TSS) and microplastics (MPs), including tire and road wear particles (TRWP). The effective removal of these pollutants is imperative to meet increasingly stringent water quality regulations. Sedimentation tanks, widely deployed for the treatment of urban runoff, are often insufficient in achieving the necessary pollutant retention efficiencies, especially for particles below 63 µm. Long-term monitoring of a sedimentation tank (mixed area with pollution categories I - III, as per DWA A-102-2) in Paderborn, Germany, verified that the actual retention efficiency for TSS63 (particles &lt; 63 µm) fell significantly below the theoretical benchmark of 49,3%%, required to meet pollution category I standards. The observed inefficiency suggests that tire wear particles, which are part of the TSS, are also insufficiently retained.To address these shortcomings, we propose a novel, cost-effective, and scalable solution in the form of a microflotation-based system. This innovative technology employs controlled microbubble generation (mean diameter:45 µm) to exploit the hydrophobic properties of microplastics, facilitating their efficient separation from water. This in-situ microflotation (ISM) technology can be easily retrofitted into existing stormwater retention basins.In this context, the focus in the presentation shown within the webinar is on the assessment of the removal of tire wear particles (TWP) in existing stormwater sedimentation tanks using in-situ microflotation. Hereby, thermal extraction desorption - gas chromatography/mass spectrometry (TGA TED-GC/MS) analysis was performed regarding the qualification and quantification of tire wear particles, as it is a state-of-the-art technology for this purpose.Within the long-term monitoring of the sedimentation tank mentioned, TGA TED-GC/MS measurements show that tire wear retention efficiencies can be strongly increased by using microflotation. Hence, the proposed system provides a benchmark for sustainable and efficient water treatment technologies, aligning with contemporary goals of resource efficiency and environmental protection.<h4>Summary of contents</h4><ul><li>Water treatment</li><li>Microplastic pollution</li><li>Qualification and quantification of tire wear particles were performed using TGA TED-GC/MS</li><li>Monitoring of sedimentation tank</li><li>Pilot-scale in-situ microflotation</li><li>Retention efficiency</li></ul><div><div data-advanced-option="false" data-has-preview-image="false" data-video-id="" data-component-id="2110920040"><div style="max-width:1px;width:100%;" id="mediaContainerWrapper_2110920040"><div style="height:1px;width:1px;" data-external-url="" id="mediaContainer_2110920040" data-advanced-option="false" data-width="1" data-height="1" data-video-id="">&nbsp;</div></div></div></div></header><div><div><div data-advanced-option="false" data-has-preview-image="false" data-video-id="" data-component-id="2110920040"><blockquote><div>Presenter: Thomas Kramer (Product Manager Laboratory, Mettler-Toledo GmbH)</div></blockquote><blockquote><div>Presenter: Dr. Mike Wenzel (Chemist for Microplastic Analysis, MicroBubbles GmbH)</div></blockquote><blockquote><div>Presenter: Maikel Haferkamp (Application Specialist, GERSTEL GmbH &amp; Co. KG)</div></blockquote></div></div></div>

Mettler Toledo: Removal of tire wear in stormwater tanks - In Situ Microflotation with TGA TED-GC/MS Analysis

Removal of tire wear in stormwater tanks: In Situ Microflotation with TGA TED-GC/MS Analysis

Capture microplastics from urban runoff. Discover how in-situ microflotation increases tire wear retention and how TGA TED-GC/MS analyzes TRWP in water

Tire Wear Removal in Stormwater: In-Situ Microflotation

The word, the phenomenon, that unpleasant tingling sensation it triggers in many people’s necks. Mass spectrometry. Some love working with it; others hate it. Yet this state-of-the-art detection technique offers not only the potential for frustration, but also many opportunities and a wide range of applications in today’s world of instrumental analysis. We want to help you overcome your fears and preconceptions and show you the theory behind the technology, what the common abbreviations mean, and how you can tackle common problems more effectively with the help of our products.<h5>Key learnings:</h5><ul><li>Mass spectrometry is powerful but often perceived as complex.</li><li>Understanding the basics and terminology makes MS more accessible.</li><li>The right tools help solve common MS challenges more efficiently.</li></ul>

Restek Corporation: Mass Spectrometry - Can It Really Be So Easy? 

EMEA live | Mass Spectrometry - Can It Really Be So Easy? 

Overcome the complexity of mass spectrometry. Master MS theory, understand key terminology, and learn to solve common analytical challenges

Mass Spectrometry - Can It Really Be So Easy? 

Discover how adopting hydrogen carrier gas maintains analytical performance and improves laboratory efficiency compared to helium.<h5>By attending this presentation &nbsp;you will:</h5><ul><li>Understand the practical steps and safety considerations for converting TD-GC-MS methods to hydrogen carrier gas, including the use of on-demand generators.</li><li>Learn how to maintain method validity, spectral integrity, and regulatory compliance during the switch.</li><li>Discover how hydrogen's chromatographic properties can reduce analytical cycle times by up to 40% and increase daily sample throughput.</li><li>Review real-world case studies demonstrating consistent, reliable results across varied applications, from environmental air monitoring to routine textile testing.</li></ul><h4>Event Overview</h4>Laboratories worldwide are facing unprecedented challenges with helium carrier gas due to soaring prices and supply chain instability. As a finite resource with a high carbon footprint, helium is prompting analytical chemists to seek sustainable, cost-efficient alternatives. Hydrogen offers a proven, practical solution that can be generated on-demand.However, transitioning a validated gas chromatography-mass spectrometry (GC-MS) method raises important questions. In this webinar, leading GC-MS experts from TESTEX, Agilent, and Markes will share their real-world experiences of migrating their GC-MS, and sample introduction systems such as thermal desorption, from helium to hydrogen. The presentation will address common analytical concerns head-on, including system safety, spectral fidelity for library matching and method validation.The speakers will demonstrate how adopting hydrogen carrier gas maintains analytical performance and actively enhances laboratory efficiency compared to helium. Attendees will see data showcasing equivalent or increased sensitivity and chromatographic resolution, and significantly faster run times that boost overall sample throughput.<h4>Who should attend?</h4><ul><li>Laboratory managers and directors looking to control operational costs and future-proof their gas supply.</li><li>Analytical chemists and GC-MS operators seeking practical advice on method translation and validation.</li><li>Quality assurance professionals focused on maintaining regulatory compliance during instrument updates.</li></ul><h4>What you need to know</h4>Start times:<ul><li>Broadcast 1: 10 am BST (London/Lisbon) / 11 am &nbsp;CEST (Paris/Berlin) &nbsp;/ 2:30 pm IST (New Delhi)</li><li>Broadcast 2: 9 am PDT (Los Angeles) / 11 am CDT (Chicago) / 12 pm EDT (New York)</li></ul>Duration: Approximately 1 hour<blockquote>Presenter: Anika Maier (Technical Expert, TESTEX AG, Zurich, Switzerland)Anika Maier is a Technical Expert at TESTEX AG in Zurich, Switzerland, an international testing and certification institute specializing in textile and leather testing, product safety, sustainability, and OEKO-TEX certifications.Building on her background as a laboratory technician, with previous roles at Celerion and SGS Institut Fresenius, and her studies in Chemical Engineering, Anika has developed extensive expertise in gas chromatography, VOC method development, thermal desorption, and emissions testing. She is highly experienced with a wide range of instrumentation, including TD, GC-MS/MS, LC-MS/MS, HRMS, and ICP-MS.Her current work focuses on laboratory optimization, troubleshooting analytical workflows, and driving automation and innovation projects. Notably, this includes the development and validation of analytical methods for textiles, consumer products, and chemical emissions, as well as successfully implementing hydrogen as a carrier gas for routine TD-GC-MS, GC-MS and GC-MS/MS applications.</blockquote><blockquote>Presenter:&nbsp;Jess Berger (Group Product Manager, Markes, UK)Jess is the Product Manager for the Markes Group, covering thermal desorption and sample introduction technologies for GC-MS. She joined Markes in 2012 after earning her chemistry degree from the University of Glamorgan (now the University of South Wales). Starting her career in inside sales, she progressed to become Product Manager. Jess’ extensive experience, combined with a recent MSc in International Innovation Management from the University of Wales Trinity Saint David, has given her a comprehensive understanding of the technology and its many benefits for GC analysis.</blockquote><blockquote>Presenter:&nbsp;Andreas Kapaklis (GC and GC-MS Product Specialist (CH/A), Agilent)Dr Andreas Kapaklis is a GC and GC-MS Product Specialist for Switzerland and Austria at Agilent Technologies. Prior to joining Agilent in early 2026, he spent eight years as a Laboratory Team Leader at TESTEX in Zurich. There, he led a team of technicians and was responsible for the laboratory's GC-MS, GC-MS/MS, and GC-HRMS systems. From 2011 to 2018, he served as Head of Quality Control and Management at the Schuler wine house in Central Switzerland. He holds a PhD in the analysis of wine aroma from Justus Liebig University Giessen. &nbsp;</blockquote>

MARKES: A Practical Guide to Switching from Helium to Hydrogen Carrier Gas

Safeguarding GC-MS Workflows: A Practical Guide to Switching from Helium to Hydrogen Carrier Gas

Future-proof your GC-MS and thermal desorption workflows. Learn how to safely switch from helium to hydrogen carrier gas and boost sample throughput by 40%.

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