LECO MOSH/MOAH Workshop - Fundamentals On Comprehensive MOSH/MOAH Analysis – Day 1

With these fundamentals you will learn all the ins and outs on the LC-GC coupling, GC×GC and Time-of-Flight MS to able to determine MOSH and MOAH in food products.

Day one on 28th April, 02.00 pm (CEST) features:

1. "Fundamentals on LC-GC Coupling"

The analysis of MOSH/MOAH is very highly complex and has several challenges. MOSH/MOAH is not a single analyte, it is a complex mixture of different compounds. The composition of this mixture is always different. Another challenge is, that natural compounds in the samples are coeluting with the mineral oil compounds and have to be removed before getting a good result. The lecture covers following topics:

• Background of MOSH/MOAH and contamination sources
• Analysis of MOSH/MOAH using LC-GC-FID
• Technology of the LC-GC coupling
• Sample clean up using automated epoxidation
• Sample cleanup using automated AlOx cleanup
• Strategies for the data analysis for LC-GC-FID data
• Coupling of LC-GC-FID system with other techniques
• Summary

Presenter: Dr. Andreas Bruchmann (Axel Semrau GmbH & Co KG, Managing Director, Sprockhövel, Germany)

After studying Analytical Chemistry at the University of Bochum, Andreas Bruchmann joined Axel Semrau in 1992. He worked in different departments before becoming Managing Director in 2010. Now he is responsible for the business unit Chromatography, which supplies worldwide automated application solutions for the food and pharmaceutical industry. He was involved in the development of the CHRONECT MOSH/MOAH Workstation from the beginning in 2010.

2. "Principles and Instrumentation of GC×GC and ToF-MS"

Comprehensive two-dimensional gas chromatography, or GC×GC, is a relatively new form of chromatography in which every peak eluting from the first column is subjected to a very fast separation on a second column with a different stationary phase. In this way two dimensional separations are obtained where peaks that coeluted on the first column are separated on the second. This gives the technique a unique separation power. Additional advantages include an improved sensitivity and group-type selectivity. It is this latter feature that makes GC×GC ideal for MOSH/MOAH separations. The saturated species can be separated from the unsaturated or aromatic ones.

Because the peaks generated in GC×GC are extremely narrow, very fast detectors are needed. Peak widths can be as low as 0.2 seconds at base-line, requiring detectors that have scan rates of 100 Hz or more. This is not a problem for the flame-ion-ization detector (FID), but it requires the use of fast scanning time-of-flight (ToF) mass spectrometers. With such devices scan rates as high as 500 Hz can be obtained. In ToF-MS devices short pulses of ions are shot into a flight tube and the time it takes for the ions to reach the end of the tube is measured. Lighter ions travel fast and have a relatively short flight time whereas the heavier ions require longer to traverse the tube. The principles and optimization of ToF MS analysers and their combination with GC×GC will be discussed with special attention for the compromise between scan speed, sensitivity and mass resolution.

Presenter: Prof. Dr. Ir. Hans-Gerd Janssen (University of Wageningen, Laboratory for Organic Chemistry and Unilever R&D, Wageningen, the Netherlands)

This Workshop is free of charge and will continue on Day 29th April, 02:00 pm (CEST) with the presentation by Prof. Giorgia Purcaro

"LC-GC×GC–ToF-MS/FID: A Powerful Platform for MOSH&MOAH Determination".

Please register also for the second day of the MOSH/MOAH Workshop!