The Might of MIKRO: Rethinking Routine Analytical Workflows through Volume Reduction and Solvent Consumption

Traditional analytical workflows often involve sample preparation techniques requiring the use of large volumes of raw samples and organic solvents. The goal is to drive up recovery and sensitivity as it pertains to obtaining low levels of analyte detection.

Modern SPE technology has emerged to minimize sample and solvent consumption, while providing analytical sensitivity that is comparable to traditional workflows. The Biotage® MIKRO plate incorporates a common 96-well plate format for high throughput analysis and a robust, loosely packed 2 mg polymeric sorbent for the extraction of a variety of analytes.

Presented here is a brief overview of common but essential workflows in a microelution format, fully automated on the Biotage® Extrahera LV-200 sample preparation instrument. Workflows are simple, cost effective, and include discussions of drugs of abuse, steroids, catecholamines, and metanephrines.

Attendees will:

• learn the unique differences between microelution and conventional SPE
• better understand the application benefits of Mikro SPE
• learn how to troubleshoot to improve analyte recovery
• gain a direct comparison of the performance of traditional SPE workflow vs Mikro SPE automated workflow

Presenter: Amber Cain (Application Scientist, Biotage)

Amber has 10 years of experience, largely involved in contract research organization work from bioanalytical pharmaceutical testing and metabolomics. She has extensive experience with SPE and other sample preparation techniques as well as LC-MS/MS.