PCBs/pesticides - Single column separation of pesticides and PCBs
Applications | 2011 | Agilent TechnologiesInstrumentation
The simultaneous separation of PCBs and a wide range of pesticides is essential for environmental monitoring and regulatory compliance. Conventional methods often require multiple columns or analyses, increasing complexity and costs.
This work evaluates the Agilent CP-Sil 5/C18 column as a single-column solution capable of resolving 30 target organochlorine compounds under a one-dimensional capillary GC method with electron capture detection.
Technique: Capillary gas chromatography
Column: Agilent CP-Sil 5/C18, 0.25 mm × 50 m, df = 0.1 μm, plus retention gap
Carrier Gas: Helium at 1 mL/min
Oven Program: 90 °C (3 min) → 170 °C at 20 °C/min, hold 5 min; → 215 °C at 2 °C/min; → 260 °C at 5 °C/min, hold 6 min
Injector: On-column at 90 °C
Detector: Electron capture (ECD) at 275 °C
Sample: 4 μL injection of 50 μg/L standard in heptane
The method achieved baseline resolution for all analytes including trifluralin, HCH isomers, hexachlorobenzene, heptachlor, ten PCB congeners (28, 31, 52, 101, 118, 138, 153, 180, 194), DDT/DDE/DDD isomers, endosulfan, dieldrin, endrin and prochloraz. The CP-Sil 5/C18 phase provided unique selectivity compared to methyl or phenyl siloxane phases.
The single-column approach simplifies workflow and reduces analysis time and cost. High selectivity and sensitivity make the method suitable for routine environmental surveillance, quality assurance and regulatory laboratories.
Emerging fast-GC techniques and mass spectrometry detection could further enhance throughput and compound identification. Automated sample preparation and advanced data processing will support high-volume environmental screening.
The Agilent CP-Sil 5/C18 column demonstrates a reliable and efficient single-column solution for simultaneous GC analysis of PCBs and pesticides, offering significant advantages for routine environmental monitoring.
Agilent Technologies, Inc. Application Note A01506, 2011.
GC, GC columns, Consumables
IndustriesEnvironmental
ManufacturerAgilent Technologies
Summary
Importance of the Topic
The simultaneous separation of PCBs and a wide range of pesticides is essential for environmental monitoring and regulatory compliance. Conventional methods often require multiple columns or analyses, increasing complexity and costs.
Objectives and Study Overview
This work evaluates the Agilent CP-Sil 5/C18 column as a single-column solution capable of resolving 30 target organochlorine compounds under a one-dimensional capillary GC method with electron capture detection.
Methodology and Instrumentation
Technique: Capillary gas chromatography
Column: Agilent CP-Sil 5/C18, 0.25 mm × 50 m, df = 0.1 μm, plus retention gap
Carrier Gas: Helium at 1 mL/min
Oven Program: 90 °C (3 min) → 170 °C at 20 °C/min, hold 5 min; → 215 °C at 2 °C/min; → 260 °C at 5 °C/min, hold 6 min
Injector: On-column at 90 °C
Detector: Electron capture (ECD) at 275 °C
Sample: 4 μL injection of 50 μg/L standard in heptane
Key Results and Discussion
The method achieved baseline resolution for all analytes including trifluralin, HCH isomers, hexachlorobenzene, heptachlor, ten PCB congeners (28, 31, 52, 101, 118, 138, 153, 180, 194), DDT/DDE/DDD isomers, endosulfan, dieldrin, endrin and prochloraz. The CP-Sil 5/C18 phase provided unique selectivity compared to methyl or phenyl siloxane phases.
Benefits and Practical Applications
The single-column approach simplifies workflow and reduces analysis time and cost. High selectivity and sensitivity make the method suitable for routine environmental surveillance, quality assurance and regulatory laboratories.
- Comprehensive separation of 30 analytes in one run
- Robust performance for trace-level detection
- Minimal method development for complex mixtures
Future Trends and Opportunities
Emerging fast-GC techniques and mass spectrometry detection could further enhance throughput and compound identification. Automated sample preparation and advanced data processing will support high-volume environmental screening.
Conclusion
The Agilent CP-Sil 5/C18 column demonstrates a reliable and efficient single-column solution for simultaneous GC analysis of PCBs and pesticides, offering significant advantages for routine environmental monitoring.
Reference
Agilent Technologies, Inc. Application Note A01506, 2011.
Content was automatically generated from an orignal PDF document using AI and may contain inaccuracies.
Similar PDF
Pesticides and PCBs
2011|Agilent Technologies|Applications
Pesticides and PCBs Application Note Environmental Authors Introduction Agilent Technologies, Inc. Gas chromatography with an Agilent CP-Sil 8 CB for Pesticides column separates 27 chloropesticides and polychlorinated biphenyls in 22 minutes. Conditions Peak identification Technique : GC-capillary Column : Agilent…
Key words
pcbs, pcbscourtesy, courtesypesticides, pesticidesprinted, printedinjector, injectorusa, usaauthors, authorsenvironmental, environmentalsize, sizetechnique, techniquecarrier, carrieridentification, identificationdetector, detectornote, notepeak
PAHs, chlorinated hydrocarbons, pesticides - Large volume injection of surface water extract
2011|Agilent Technologies|Applications
PAHs, chlorinated hydrocarbons, pesticides Large volume injection of surface water extract Application Note Environmental Authors Introduction Agilent Technologies, Inc. An example of LVI in environmental analytical chemistry is the trace analysis of aromatics, chlorinated hydrocarbons and pesticides in surface water.…
Key words
lvi, lvienvironmental, environmentallarge, largeidentification, identificationcourtesy, courtesyvolume, volumeguard, guardpeak, peakchemistry, chemistrypesticides, pesticidesprinted, printedextract, extractinjector, injectorexample, examplesurface
Determination of pesticides and persistent organic pollutants in honey by accelerated solvent extraction and GC-MS/MS
2017|Thermo Fisher Scientific|Applications
CUSTOMER APPLICATION NOTE Fabrizio Galbiati1, Sara Panseri2, Giuseppe Labella2, Annamaria Giorgi2, Radmila Pavlovic2, Sonia Bonacci2, Francesco Arioli2, Luca Chiesa2; 1 Thermo Fisher Scientific (Schweiz) AG, Reinach, Switzerland; 2University of Milan, Faculty of Veterinary Medicine, Milan, Italy Keywords: Pressurized fluid extraction,…
Key words
honey, honeytrentino, trentinoextraction, extractionheptachlor, heptachlorpesticides, pesticidesendosulfan, endosulfanase, asehch, hchaccelerated, acceleratedcalabria, calabriaalto, altopcbs, pcbsfrom, fromfish, fishitaly
Determination of pesticides and persistent organic pollutants in honey by accelerated solvent extraction and GC-MS/MS
2017|Thermo Fisher Scientific|Applications
CUSTOMER APPLICATION NOTE Fabrizio Galbiati1, Sara Panseri2, Giuseppe Labella2, Annamaria Giorgi2, Radmila Pavlovic2, Sonia Bonacci2, Francesco Arioli2, Luca Chiesa2; 1 Thermo Fisher Scientific (Schweiz) AG, Reinach, Switzerland; 2University of Milan, Faculty of Veterinary Medicine, Milan, Italy Keywords: Pressurized fluid extraction,…
Key words
honey, honeytrentino, trentinoextraction, extractionpesticides, pesticidesheptachlor, heptachlorendosulfan, endosulfanase, asehch, hchaccelerated, acceleratedcalabria, calabriaalto, altopcbs, pcbsfrom, fromfish, fishitaly
