LUMOS II FTIR Imaging Microscope

Significance of the Topic

Infrared microscopy is a powerful analytical approach that combines the spatial resolution of optical microscopy with the molecular specificity of FTIR spectroscopy. By generating a full spectrum at each pixel, it enables detailed chemical mapping of materials across a range of fields including polymers, pharmaceuticals, electronics, environmental science and life sciences. Recent advances aim to make this technology more accessible to non-experts while maintaining high throughput and reliable performance.

Objectives and Study Overview

This article reviews the design and capabilities of the LUMOS II FTIR imaging microscope, highlighting its goal of simplifying microanalysis workflows. Emphasis is placed on user-centric automation, rapid data acquisition, flexible sampling modes, and compliance with industry regulations to ensure consistent results in research, QA/QC and routine laboratory applications.

Methodology

FTIR imaging was performed in transmission, reflection and attenuated total reflection (ATR) modes. A focal-plane array detector acquires complete spectra across a wide field of view, while a retractable ATR crystal with piezo-driven control ensures precise sample contact. The PermaSure+ system provides pixel-level laser wavenumber calibration for stable and accurate spectral data. Automated motorized stages and software-driven aperture, detector and measurement mode changes streamline method setup.

Used Instrumentation

• LUMOS II FTIR Imaging Microscope (Bruker Optics)
• Focal-Plane Array (FPA) detector with 0.6 µm/pixel spatial resolution
• TE-MCT detector (no liquid nitrogen needed), with optional DTGS and LN-cooled MCT detectors
• Retractable ATR crystal with germanium hemisphere for sticky or soft samples
• Automated sample stage with large working distance and 40 mm height capacity
• PermaSure+ laser calibration system
• ALPHA II macro accessory for complementary ATR and transmission sampling

Main Results and Discussion

The LUMOS II achieves ultrafast imaging over areas up to 1490 × 1118 µm2, producing high-definition chemical maps within minutes. PermaSure+ calibration yields flat, accurate spectral baselines across all pixels. The instrument operates without purge or liquid nitrogen, reducing downtime and maintenance. User guided software simplifies measurement protocols while ensuring regulatory compliance (cGMP, GLP, 21 CFR Part 11). Application examples demonstrate its ability to detect polymer inclusions, map API distribution in tablets, analyze electronics failures, assess automotive coatings, identify tissue biomarkers and quantify microplastics in environmental samples.

Benefits and Practical Applications

• Rapid, high-resolution chemical imaging in diverse matrices
• Automated workflows reduce user errors and accelerate training
• Flexible measurement modes address multiple sample types
• Regulatory compliance supports pharmaceutical and quality control labs
• Elimination of purge systems and liquid nitrogen lowers operating costs
• Compact footprint adapts to crowded laboratory environments

Future Trends and Opportunities

Ongoing developments include integration of advanced detectors for higher sensitivity, AI-driven data analysis to automate spectral interpretation, and expanded macro-micro coupling for seamless sample characterization. Emerging applications in cell biology, materials science and environmental monitoring will benefit from even faster acquisition, intelligent imaging protocols and cloud-based data management. The trend towards user-friendly interfaces and turnkey solutions will further democratize FTIR imaging.

Conclusion

The LUMOS II FTIR imaging microscope exemplifies a new generation of user-oriented spectroscopic tools. By combining high-speed FPA imaging, comprehensive automation, reliable calibration and broad sampling flexibility, it enables detailed molecular analysis across many applications with minimal expertise required. Its low operating requirements and regulatory compliance make it an integral asset for modern analytical laboratories.

References

• Bruker Optics. LUMOS II FTIR Imaging Microscope Whitepaper, BOPT-4001283-01, 2019.
• Patents: DE 102004025448; DE 19940981; DE 102012200851B3; US 8873140B2.