Konference CEEPC/IPM/CMSC 2022 - Sborník abstraktů

Significance of Topic

Proteomics and mass spectrometry have emerged as cornerstone technologies for dissecting complex biological systems, enabling the identification and quantification of proteins, metabolites, and their modifications with high sensitivity and resolution. These methods impact basic research, precision medicine, and industrial analytics by revealing molecular details that drive cellular function, disease mechanisms, and biomarker discovery.

Objectives and Overview

This volume compiles advances in methodology development, instrument innovations, software tools, and diverse applications in analytical and biomedical proteomics. It showcases new experimental workflows, instrumentation performance enhancements, and multi-omics strategies that improve throughput, sensitivity, and data interpretation.

Methodology and Instrumentation

Contributors present a range of cutting-edge techniques including:

• Nano- and microflow liquid chromatography coupled to high-resolution mass spectrometers (QTOF, Orbitrap, FT-ICR, Cyclic IMS)
• Trapped ion mobility (TIMS) and cyclic ion mobility spectrometry for detailed conformational analyses
• Sample preparation platforms for single-cell and intact protein studies (proteoCHIP, immunoassays, SPE, ultrafiltration)
• Data-independent acquisition modes (dia-PASEF, SWATH) and advanced fragmentation (CID, EAD, 2DMS)
• Automated high-throughput platforms and software tools for peptide and small-molecule quantification

Main Findings and Discussion

Key discoveries and insights include:

• Single-cell proteomic workflows achieving identification of >1,500 proteins per cell with minimal carrier proteome
• Novel approaches for intact protein and antibody characterization using liquid chromatography-MS and capillary electrophoresis
• Enhanced phosphoproteome coverage in short gradients and low-sample loads via TIMS-enabled dia-PASEF
• Multi-omics applications uncovering biomarkers in cancer (renal carcinoma, heart failure), neurodegeneration (tauopathies, Alzheimer’s), infectious diseases, and cardiac amyloidosis
• Use of endogenous metabolites (NAD, RNA caps) and extracellular vesicles for diagnostic signatures

Benefits and Practical Applications

The described innovations unlock high-throughput, sensitive, and multiplexed measurements, supporting:

• Development of non-invasive biomarkers and on-site diagnostics (tears, sweat, urine)
• Real-time monitoring of therapeutic antibodies, toxins, and small-molecule drugs
• Precise mapping of protein interactions and post-translational modifications for drug target validation
• Enhanced forensic and cultural heritage analysis (fur authenticity, indigo provenance)

Future Trends and Possibilities

Emerging directions highlighted include single-cell multi-omics integration, AI-driven data interpretation, advanced cryogenic and gas-phase structural MS, and portable ambient ionization for field applications. These approaches will further push the limits in spatial resolution, dynamic range, and speed.

Conclusion

This compilation underscores the rapid evolution of mass spectrometry-based analytical platforms and workflows that collectively drive deeper molecular insights across life sciences and translational research. Continued innovation in instrumentation, sample preparation, and computational analysis will expand both fundamental understanding and practical applications in diagnostics, therapeutics, and beyond.

References

Key methodologies and applications are discussed in leading journals such as Nature Methods, Analytical Chemistry, Journal of Proteome Research, and others, illustrating the broad impact and ongoing progress in the field.