Trace Detection of Rhodamine B in Cayenne Powder
Applications | 2020 | MetrohmInstrumentation
The contamination of food products with unauthorized dyes poses a serious risk to consumer health and undermines food safety regulations. Rhodamine B is a synthetic dye banned in many regions due to its potential toxicity. Developing rapid, on-site screening techniques is critical for safeguarding the supply chain and ensuring regulatory compliance.
The goal of this study was to demonstrate the capability of the Metrohm Instant SERS Analyzer (MISA) for detecting trace amounts of Rhodamine B in ground cayenne pepper. The work involved preparing spiked samples to establish detection limits and evaluating a simple extraction and measurement protocol suitable for field use.
Samples of commercial cayenne pepper were spiked with known concentrations of Rhodamine B and air-dried. A methanolic extraction followed by mixing with gold nanoparticle colloid and salt solution enabled surface-enhanced Raman scattering (SERS) measurements. Key instrumentation:
SERS spectra obtained from spiked cayenne samples revealed clear Rhodamine B signals down to 50 μg/g with baseline subtraction of the native pepper matrix. Introducing a simple concentration step by evaporating and resuspending extracts improved the detection limit to 10 μg/g. The study confirmed high reproducibility across replicates and minimal sample consumption.
The proposed approach offers:
Advances may include extending the SERS library to other banned dyes, integrating automated sample handling, and coupling the analyzer with cloud-based databases for real-time data sharing. Further miniaturization and multiplexed detection schemes could broaden applications in food safety and environmental monitoring.
This work demonstrates that MISA provides a reliable, efficient solution for trace detection of Rhodamine B in food matrices. The method's simplicity, portability, and sensitivity make it an attractive tool for regulatory agencies and quality control laboratories.
RAMAN Spectroscopy
IndustriesFood & Agriculture
ManufacturerMetrohm
Summary
Significance of the Topic
The contamination of food products with unauthorized dyes poses a serious risk to consumer health and undermines food safety regulations. Rhodamine B is a synthetic dye banned in many regions due to its potential toxicity. Developing rapid, on-site screening techniques is critical for safeguarding the supply chain and ensuring regulatory compliance.
Objectives and Study Overview
The goal of this study was to demonstrate the capability of the Metrohm Instant SERS Analyzer (MISA) for detecting trace amounts of Rhodamine B in ground cayenne pepper. The work involved preparing spiked samples to establish detection limits and evaluating a simple extraction and measurement protocol suitable for field use.
Methodology and Instrumentation
Samples of commercial cayenne pepper were spiked with known concentrations of Rhodamine B and air-dried. A methanolic extraction followed by mixing with gold nanoparticle colloid and salt solution enabled surface-enhanced Raman scattering (SERS) measurements. Key instrumentation:
- Metrohm Instant SERS Analyzer (MISA Advanced with Orbital-Raster-Scan spectrograph)
- Gold nanoparticle ID Kit (Au NP colloid, pipettes, vials)
- Mobile sampling attachments for vial-based analysis
- Software: MISA Cal V1.0.15, Firmware 0.9.33
Main Results and Discussion
SERS spectra obtained from spiked cayenne samples revealed clear Rhodamine B signals down to 50 μg/g with baseline subtraction of the native pepper matrix. Introducing a simple concentration step by evaporating and resuspending extracts improved the detection limit to 10 μg/g. The study confirmed high reproducibility across replicates and minimal sample consumption.
Benefits and Practical Applications of the Method
The proposed approach offers:
- Rapid on-site screening without extensive sample preparation
- Portable instrumentation suitable for field or mobile lab use
- High sensitivity for trace-level detection
- Cost-effective workflow with minimal reagent use
Future Trends and Applications
Advances may include extending the SERS library to other banned dyes, integrating automated sample handling, and coupling the analyzer with cloud-based databases for real-time data sharing. Further miniaturization and multiplexed detection schemes could broaden applications in food safety and environmental monitoring.
Conclusion
This work demonstrates that MISA provides a reliable, efficient solution for trace detection of Rhodamine B in food matrices. The method's simplicity, portability, and sensitivity make it an attractive tool for regulatory agencies and quality control laboratories.
Content was automatically generated from an orignal PDF document using AI and may contain inaccuracies.
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