Biotage Extrahera LV-200 - User Manual

Biotage Extrahera LV-200 Automated Sample Preparation System: Comprehensive Overview

Importance of the Topic

• Automated sample preparation is critical for high-throughput, reproducible, and error-free analytical workflows in pharmaceutical, environmental, and food testing laboratories.
• The Biotage Extrahera LV-200 addresses common challenges in manual extraction, including variability, cross-contamination, and limited throughput.
• Integration of multiple extraction techniques on a single platform enhances flexibility and resource efficiency.

Aim and Overview of the Study

• To present the design, capabilities, and operational workflow of the Biotage Extrahera LV-200.
• To illustrate how the system supports supported liquid extraction (SLE), solid phase extraction (SPE), protein precipitation (PPT), phospholipid depletion (PLD), dual mode extraction, and dual flow extraction (DFE).
• To highlight configurable GLP features for laboratories with regulatory requirements.

Methodology and Instrumentation

• Workflow automation is achieved via a robotic pipetting module equipped with replaceable 50 μL, 200 μL, and 1 mL tips and optional DFE column tips.
• Sample preparation steps include conditioning, equilibration, sample load, wash, elution, drying, and collection, each programmable with specific volumes, flow rates, and pressure gradients.
• The carousel and lifter module handles up to four collection plates or flow-through plates, preventing cross-contamination via sealed Luer fittings.

Used Instrumentation

• Biotage Extrahera LV-200 automated platform with integrated solvent pumps (S1–S5) and optional manual reservoir rack (position 6).
• Pressure unit supporting ISO-compliant gas quality and flow control for SPE, SLE, and DFE.
• Vacuum interface for waste collection and a 5 L external waste reservoir.
• 12" adjustable touch screen with optional barcode/QR scanner and GLP software module.

Main Results and Discussion

• The system delivers consistent sample processing across 96-well plates and column racks with minimal user intervention.
• Smart Sample Loading and pressure-controlled drying reduce cycle times and improve solvent usage efficiency.
• GLP audit trails, user roles, and network integration support data integrity and remote monitoring.

Benefits and Practical Applications of the Method

• High reproducibility and reduced manual handling errors benefit QA/QC in pharmaceutical and environmental labs.
• Flexible method development supports diverse matrices and workflows, from simple protein precipitation to advanced DFE protocols.
• Modular design allows scaling from routine screening to complex, multi-step sample preparation without additional instrumentation.

Future Trends and Possibilities

• Integration with laboratory information management systems (LIMS) and cloud-based data analytics for fully connected workflows.
• Adoption of greener solvents and miniaturized consumables to reduce waste and environmental impact.
• Application of artificial intelligence for dynamic method optimization and real-time troubleshooting.

Conclusion

• The Biotage Extrahera LV-200 provides a versatile, robust solution for automated sample preparation across multiple extraction techniques.
• Its advanced features, including pressure gradients, DFE mode, and GLP support, position it as a valuable tool for modern analytical laboratories seeking efficiency, reproducibility, and regulatory compliance.

References

1. Biotage Extrahera LV-200 User Manual, Biotage Sweden AB, 2021.