EVOLUTE® EXPRESS - Solid Phase Extraction Columns

Significance of the Topic

Solid phase extraction is widely used for sample preparation in bioanalysis environmental testing and industrial quality control. The ability to streamline and accelerate SPE workflows supports high throughput labs reducing turnaround times and improving reliability.

Study Objectives and Overview

This article introduces EVOLUTE EXPRESS SPE columns which integrate established sorbent chemistries with optimized hardware for an expedited load wash elute workflow. The aim is to eliminate conditioning and equilibration steps thereby improving processing speed consistency and productivity.

Methodology and Instrumentation

The performance of EVOLUTE EXPRESS columns was evaluated against a leading competitor using a standard load wash elute protocol. Pooled human urine samples diluted with water were processed under 1 psi pressure on a Biotage Pressure plus instrument. Key performance metrics included flow rates analyte recoveries and total processing time for 96 samples.

Used Instrumentation

• Biotage Extrahera automated SPE platform
• Biotage Pressure plus SPE instrument
• Vacuum manifold compatible with tabless columns

Main Results and Discussion

The EVOLUTE EXPRESS columns showed rapid and consistent flow even when loading aqueous samples onto dry sorbent beds. Sample loading completed within 15 seconds compared to over 210 seconds for the competitor product. Interference washes did not compromise analyte recovery. Eliminating conditioning and equilibration reduced total processing time for 96 samples by approximately 30 percent saving over nine minutes.

Benefits and Practical Applications

• Accelerated sample throughput supports high volume laboratories
• Consistent analyte recovery ensures reliable quantitative results
• Tabless modular design adapts to automated platforms reducing consumable costs

Future Trends and Potential Applications

As demand for rapid bioanalytical screening grows further developments may include integration with online HPLC and mass spectrometry workflows. Expanding sorbent chemistries to target emerging contaminant classes and miniaturization for microsampling are potential directions.

Conclusion

EVOLUTE EXPRESS SPE columns offer a streamlined SPE approach by removing conditioning steps while delivering fast consistent flow and reliable recoveries. The design supports high throughput operations and reduces per sample processing time making it a valuable tool for modern analytical laboratories.

Reference

Biotage EVOLUTE EXPRESS SPE Columns Application Note PPS383 2015