Thermo Scientific HyperSep Columns Application Notebook – Issue 1

Importance of the Topic

SPE is a critical technique enabling selective sample cleanup concentration and transfer to LC GC or MS systems. Thermo Scientific HyperSep columns provide a broad portfolio of sorbent phases tailored to diverse analytes offering high throughput low solvent consumption and robust recoveries across pharmaceutical environmental forensic and food safety applications.

Goals and Overview

This notebook guides users through selection and use of HyperSep SPE columns from sample pretreatment conditioning loading washing and elution to analytical detection. It showcases sorbent properties method development strategies optimization of wash and elution profiles and validated protocols for a wide range of analytes.

Methodology and Instrumentation

Core SPE workflow

• Sample pretreatment including pH adjustment dilution filtration
• Column conditioning with organic and aqueous solvents
• Column pre equilibration
• Sample loading at controlled flow rate
• Selective washing to remove interferences
• Analyte elution by solvent strength or pH change

Used Instrumentation

• Positive pressure and vacuum SPE manifold
• Nitrogen evaporator or gentle stream evaporator
• Probe sonicator and centrifuge
• HPLC and UHPLC systems with UV PDA detectors
• GC MS and tandem GC MS MS systems
• LC MS MS triple quadrupole

Main Results and Discussion

Optimized wash and elution conditions achieved high recoveries for various phases demonstrating phase dependent selectivity and efficient matrix cleanup. Typical recoveries exceeded 80 to 95 percent with minimal co extraction of interferences.

Benefits and Practical Applications

HyperSep columns deliver

• Enhanced sensitivity through concentration and cleanup
• Broad chemical compatibility and pH stability
• Reduced solvent and sample consumption
• Improved instrument performance and data consistency
• Extensive application coverage in pharmaceutical environmental forensic and food safety testing

Future Trends and Opportunities

Emerging directions include online and automated SPE integration micro SPE formats greener solvent systems novel hybrid sorbents for ultra polar analytes and high throughput SPE LC MS workflows for bioanalysis and environmental screening.

Conclusion

The Thermo Scientific HyperSep portfolio offers versatile optimized SPE solutions that streamline sample preparation enhance analytical performance and ensure robust reproducibility across regulatory compliant workflows.

References

Thermo Scientific HyperSep Columns Application Notebook Issue 1 April 2011