Accurately Quantify 380 Pesticides Using LC-MS/MS and GC-MS/MS

Importance of topic

Certified reference materials and robust analytical methods are essential for ensuring accurate detection and quantification of pesticide residues in food and environmental samples. Regulatory requirements worldwide demand reliable multiresidue screening capable of addressing hundreds of compounds at trace levels. CRM kits combined with advanced LC-MS/MS and GC-MS/MS instrumentation support method validation, quality assurance, and compliance.

Objectives and study overview

This summary outlines Restek’s multiresidue pesticide Certified Reference Material (CRM) kits designed for LC-MS/MS and GC-MS/MS, focusing on:

• Comprehensive coverage of 380 pesticides grouped by chemical class for stability and ease of use
• Quantitative confirmation of kit composition and provision of detailed documentation
• Delivery of optimized multiresidue methods and transition tables free of charge
• ISO-accredited manufacturing and QC to satisfy quality management standards

Methodology and instrumentation

Restek’s approach integrates:

• Formulation of 204-analyte LC-MS/MS kits and 203-analyte GC-MS/MS kits grouped into 10 and 9 mixtures respectively to maximize chemical stability
• Chromatographic separation using Ultra Aqueous C18 columns (LC) and Rxi-5ms columns (GC), achieving sub-15 min cycle times per mix
• Extraction and cleanup via QuEChERS protocols with tailored salt packets and dispersive SPE tubes for diverse matrices
• Use of isotopically labeled internal standards for accurate quantitation and compensation of matrix effects
• Supporting accessories including filter vials, leak detectors, and flowmeters for routine instrument maintenance

Main results and discussion

• Successful development of stable, high-purity multicomponent standards with verified concentration (100 µg/mL) in compatible solvents
• Chromatographic profiles demonstrate baseline separation of target analytes across chemical classes within recommended run times
• QuEChERS extraction and dSPE cleanup deliver reproducible recoveries and minimal interferences for GC-MS/MS and LC-MS/MS analyses

Benefits and practical applications

• Accelerates method setup, verification, and validation while reducing cost and time
• Supports global food safety and environmental monitoring laboratories in achieving regulatory compliance
• Ensures long-term stability and reliability of reference standards under demanding laboratory conditions
• Offers compatibility with free downloadable multiresidue methods, facilitating rapid implementation

Future trends and applications

• Expansion of CRM offerings to emerging pesticides and transformation products
• Integration with high-resolution mass spectrometry and ion mobility for enhanced selectivity
• Automation of sample preparation workflows including inline QuEChERS and filter systems
• Development of next-generation low-bleed columns and advanced stationary phases for broader analyte ranges

Conclusion

Restek’s multiresidue pesticide CRM kits for LC-MS/MS and GC-MS/MS provide a comprehensive, stable, and ISO-accredited solution for accurate quantitation of hundreds of pesticides, supported by optimized methods, quality control materials, and sample preparation consumables to meet the evolving demands of food safety and environmental laboratories.