16th MDCW 2025 - Short Course (Day 0)

A Beginner’s Guide to Multidimensional Chromatography

• Instructors: Pierre-Hugues Stefanuto, Katelynn Perrault Uptmor, Dwight Stoll, Petr Vozka

Course Description

So...you want to do multidimensional chromatography? If you are getting started in the field using either liquid or gas chromatography, you’re in the right place.

This course will cover a brief history and introduction to both multidimensional gas chromatography and multidimensional liquid chromatography.

Discussion will also include basic system components and data handling strategies to apply after acquiring your data. 

A hands-on portion of the course will also include familiarization with common system components and consumables for both GC and LC, as well as the opportunity to use a commercial GC×GC instrument.

MDCW: 16th MDCW 2025 - Short Course (Day 0) Instructors - Pierre-Hugues Stefanuto, Katelynn Perrault Uptmor, Petr Vozka, Dwight Stoll

Target Participants

This course is intended to be an initial touchpoint for those who are looking to explore multidimensional chromatography as a new option/capability, or who might be using it for the first time. The course is intended for new users to obtain a background on the techniques as a primer to the workshop.  Advanced topics will not be discussed.

SHORT COURSE SPONSORS 2025

• Acesss
• Certech

MDCW 2025: Short Course Sponsors.

Agenda

Part I – History, Terminology, Fundamental Concepts

• Brief history of the development of multidimensional separations
• Terminology
• Fundamental Concepts

MDCW: 16th MDCW 2025 - Short Course (Day 0)

Part II – Introduction to Multidimensional Gas Chromatography

• Method basics (column selection, method parameters, carrier gas choices, detector considerations)
• Modulator selection (thermal, flow, others)
• Method development made simple
• Data preprocessing and initial handling

MDCW: 16th MDCW 2025 - Short Course (Day 0)

Part III – Introduction to Multidimensional Liquid Chromatography

• Modes of multidimensional separation commonly used with LC (heartcut, multiple
  heartcut/selective comprehensive, fully comprehensive)
• Interfaces used for multidimensional LC
• Common use cases of multidimensional LC ( industry and academic research examples)
• Introduction to method development strategies

MDCW: 16th MDCW 2025 - Short Course (Day 0) Part IV – Laboratory Session

Part IV – Laboratory Session

Participants will be put in small groups and rotate to different stations to familiarize with a variety of
GC and LC components used for multidimensional systems including:

• Rotation 1: 2D LC – Interfaces, valves, columns, acquisition software
• Rotation 2: GC×GC – Tuning, sequence setup, acquisition
• Rotation 3: GC×GC – Hardware and consumables
• Rotation 4: GC×GC – Data processing examples

MDCW: 16th MDCW 2025 - Short Course (Day 0) Rotation 1 2D LC – Interfaces, valves, columns, acquisition software

MDCW: 16th MDCW 2025 - Short Course (Day 0) Rotation 2 - GC×GC – Tuning, sequence setup, acquisition

MDCW: 16th MDCW 2025 - Short Course (Day 0) Rotation 3 GC×GC – Hardware and consumables

MDCW: 16th MDCW 2025 - Short Course (Day 0) Rotation 4 - GC×GC – Data processing examples