GCMS
More information
WebinarsAbout usContact usTerms of use
LabRulez s.r.o. All rights reserved. Content available under a CC BY-SA 4.0 Attribution-ShareAlike
Author
The Multidimensional Chromatography (MDC) Workshop
The Multidimensional Chromatography (MDC) Workshop draws experts in this exciting field to share and discuss their research.
Tags
Presentation
Video
Logo of LinkedIn

Multi-omics workflow for blood serum screening using GC×GC-TOFMS (Pierre-Hugues Stefanuto, MDCW 2024)

Th, 29.2.2024
| Original article from: The Multidimensional Chromatography (MDC) Workshop
In this study, we analyzed 64 human serum samples representing three different groups of colorectal cancer using cutting edge GC×GC–LR/HR-TOFMS techniques.
Video placeholder
  • Photo: MDCW: Multi-omics workflow for blood serum screening using GC×GC-TOFMS (Pierre-Hugues Stefanuto, MDCW 2024)
  • Video: LabRulez: Pierre-Hugues Stefanuto: Multi-omics workflow for blood serum screening using GC×GC-TOFMS (MDCW 2024)

🎤 Presenter: Pierre-Hugues Stefanuto (Liege University, Liege, Belgium)

💾 PDF presentation

💡 Book in your calendar: 16th Multidimensional Chromatography Workshop (MDCW) 3 - 5. February 2025

Abstract

According to the world health organization (WHO), colorectal cancer (CRC) ranks as the third most frequently diagnosed cancer and the second leading cause of cancer-related deaths. The current endoscopic-based or stool-based diagnostic techniques are either highly invasive or lack sufficient sensitivity. Thus, there is a need for better screening approaches.

The rapid advancement of high throughputomics” approaches, such as meta-genomics, transcriptomics, proteomics, metabolomics, lipidomics, microbiomics, and volatolomics, offer a potentially less invasive alternative than available techniques to develop novel biomarkers for colorectal cancer screening that could contribute to its clinical management.

For small molecules “omics” screening, gas chromatography coupled to mass spectrometry (GC-MS) represents a method of choice to screen biofluid samples. However, GC-MS is mostly used for targeted analysis due to the high complexity of the matrices. To tackle this limitation, comprehensive two-dimensional gas chromatography coupled to mass spectrometry provided increased separation capabilities, making it a method of choice for untargeted small molecule metabolomics.

In this study, we analyzed 64 human serum samples representing three different groups of colorectal cancer using cutting edge GC×GC–LR/HR-TOFMS techniques. We analyzed samples with two different specifically tailored sample preparation approaches for lipidomics (fatty acids) (25 μL serum) and metabolomics (50 μL serum). In-depth chemometric screening with supervised and unsupervised approaches and metabolic pathway analysis were applied on both datasets. To ensure the high quality of the data produced, we have established a strict QAQC protocol including pooled sampled, serum SRM, and standard mixtures injections.

The Multidimensional Chromatography (MDC) Workshop
 

Related content

Simultaneous Analysis of Residual Pesticides Using High-Speed Scan and Smart SIM+ of GCMS-QP2050

Applications
| 2024 | Shimadzu
Instrumentation
GC/MSD, GC/SQ
Manufacturer
Shimadzu
Industries
Food & Agriculture

Agilent 8850 GC system

Brochures and specifications
| 2024 | Agilent Technologies
Instrumentation
GC
Manufacturer
Agilent Technologies
Industries
Others

GC/GC-MS Glass Insert/Liner Selection Guide

Guides
| 2024 | Shimadzu
Instrumentation
Consumables, GC, GC/MSD
Manufacturer
Shimadzu
Industries
Others

Improving Battery Production Yield, Performance, and Stability Using FTIR

Applications
| 2024 | Agilent Technologies
Instrumentation
FTIR Spectroscopy
Manufacturer
Agilent Technologies
Industries
Energy & Chemicals , Materials Testing

An advanced integrated GC-MS/MS and LC-MS/MS workflow for the comprehensive analysis of pesticide residues in food

Applications
| 2024 | Thermo Fisher Scientific
Instrumentation
GC/MSD, GC/MS/MS, GC/QQQ, LC/MS, LC/MS/MS, LC/QQQ
Manufacturer
Thermo Fisher Scientific
Industries
Food & Agriculture
 

Related articles


Article | Upcoming events

Cal State LA hosts 15th Multidimensional Chromatography Workshop

This January, Cal State LA became a hub for scientists and researchers as it hosted the 15th Multidimensional Chromatography Workshop.
Complex Chemical Composition Analysis Lab (C³AL)
more

Article | Academy

Free NIST Data Processing Software Lab for Universities - Part 10: AMDIS Difficulties and Ways to Overcome Them in Free Laboratory Resource for Universities

In the tenth part, we will look at difficulties with AMDIS and ways to overcome them
James Little/Mass Spec Interpretation Services
more

Article | Scientific article

An innovative approach to planar chiral substances

New highly efficient method for the preparation of planar chiral compounds based on organocatalytic desymmetrization was published in Nature Communications
Katedra organické chemie Přírodovědecké fakulty Univerzity Karlovy
more

Article | Software

From a scientific project to a global business venture. The company mzio is striving to improve data analysis in mass spectrometry labs

A piece of software which enables researchers worldwide to speed up their work and helps them make scientific discoveries is expanding from science into commerce.
Ústav organické chemie a biochemie AV ČR
more
 

Related content

Simultaneous Analysis of Residual Pesticides Using High-Speed Scan and Smart SIM+ of GCMS-QP2050

Applications
| 2024 | Shimadzu
Instrumentation
GC/MSD, GC/SQ
Manufacturer
Shimadzu
Industries
Food & Agriculture

Agilent 8850 GC system

Brochures and specifications
| 2024 | Agilent Technologies
Instrumentation
GC
Manufacturer
Agilent Technologies
Industries
Others

GC/GC-MS Glass Insert/Liner Selection Guide

Guides
| 2024 | Shimadzu
Instrumentation
Consumables, GC, GC/MSD
Manufacturer
Shimadzu
Industries
Others

Improving Battery Production Yield, Performance, and Stability Using FTIR

Applications
| 2024 | Agilent Technologies
Instrumentation
FTIR Spectroscopy
Manufacturer
Agilent Technologies
Industries
Energy & Chemicals , Materials Testing

An advanced integrated GC-MS/MS and LC-MS/MS workflow for the comprehensive analysis of pesticide residues in food

Applications
| 2024 | Thermo Fisher Scientific
Instrumentation
GC/MSD, GC/MS/MS, GC/QQQ, LC/MS, LC/MS/MS, LC/QQQ
Manufacturer
Thermo Fisher Scientific
Industries
Food & Agriculture
 

Related articles


Article | Upcoming events

Cal State LA hosts 15th Multidimensional Chromatography Workshop

This January, Cal State LA became a hub for scientists and researchers as it hosted the 15th Multidimensional Chromatography Workshop.
Complex Chemical Composition Analysis Lab (C³AL)
more

Article | Academy

Free NIST Data Processing Software Lab for Universities - Part 10: AMDIS Difficulties and Ways to Overcome Them in Free Laboratory Resource for Universities

In the tenth part, we will look at difficulties with AMDIS and ways to overcome them
James Little/Mass Spec Interpretation Services
more

Article | Scientific article

An innovative approach to planar chiral substances

New highly efficient method for the preparation of planar chiral compounds based on organocatalytic desymmetrization was published in Nature Communications
Katedra organické chemie Přírodovědecké fakulty Univerzity Karlovy
more

Article | Software

From a scientific project to a global business venture. The company mzio is striving to improve data analysis in mass spectrometry labs

A piece of software which enables researchers worldwide to speed up their work and helps them make scientific discoveries is expanding from science into commerce.
Ústav organické chemie a biochemie AV ČR
more
 

Related content

Simultaneous Analysis of Residual Pesticides Using High-Speed Scan and Smart SIM+ of GCMS-QP2050

Applications
| 2024 | Shimadzu
Instrumentation
GC/MSD, GC/SQ
Manufacturer
Shimadzu
Industries
Food & Agriculture

Agilent 8850 GC system

Brochures and specifications
| 2024 | Agilent Technologies
Instrumentation
GC
Manufacturer
Agilent Technologies
Industries
Others

GC/GC-MS Glass Insert/Liner Selection Guide

Guides
| 2024 | Shimadzu
Instrumentation
Consumables, GC, GC/MSD
Manufacturer
Shimadzu
Industries
Others

Improving Battery Production Yield, Performance, and Stability Using FTIR

Applications
| 2024 | Agilent Technologies
Instrumentation
FTIR Spectroscopy
Manufacturer
Agilent Technologies
Industries
Energy & Chemicals , Materials Testing

An advanced integrated GC-MS/MS and LC-MS/MS workflow for the comprehensive analysis of pesticide residues in food

Applications
| 2024 | Thermo Fisher Scientific
Instrumentation
GC/MSD, GC/MS/MS, GC/QQQ, LC/MS, LC/MS/MS, LC/QQQ
Manufacturer
Thermo Fisher Scientific
Industries
Food & Agriculture
 

Related articles


Article | Upcoming events

Cal State LA hosts 15th Multidimensional Chromatography Workshop

This January, Cal State LA became a hub for scientists and researchers as it hosted the 15th Multidimensional Chromatography Workshop.
Complex Chemical Composition Analysis Lab (C³AL)
more

Article | Academy

Free NIST Data Processing Software Lab for Universities - Part 10: AMDIS Difficulties and Ways to Overcome Them in Free Laboratory Resource for Universities

In the tenth part, we will look at difficulties with AMDIS and ways to overcome them
James Little/Mass Spec Interpretation Services
more

Article | Scientific article

An innovative approach to planar chiral substances

New highly efficient method for the preparation of planar chiral compounds based on organocatalytic desymmetrization was published in Nature Communications
Katedra organické chemie Přírodovědecké fakulty Univerzity Karlovy
more

Article | Software

From a scientific project to a global business venture. The company mzio is striving to improve data analysis in mass spectrometry labs

A piece of software which enables researchers worldwide to speed up their work and helps them make scientific discoveries is expanding from science into commerce.
Ústav organické chemie a biochemie AV ČR
more
 

Related content

Simultaneous Analysis of Residual Pesticides Using High-Speed Scan and Smart SIM+ of GCMS-QP2050

Applications
| 2024 | Shimadzu
Instrumentation
GC/MSD, GC/SQ
Manufacturer
Shimadzu
Industries
Food & Agriculture

Agilent 8850 GC system

Brochures and specifications
| 2024 | Agilent Technologies
Instrumentation
GC
Manufacturer
Agilent Technologies
Industries
Others

GC/GC-MS Glass Insert/Liner Selection Guide

Guides
| 2024 | Shimadzu
Instrumentation
Consumables, GC, GC/MSD
Manufacturer
Shimadzu
Industries
Others

Improving Battery Production Yield, Performance, and Stability Using FTIR

Applications
| 2024 | Agilent Technologies
Instrumentation
FTIR Spectroscopy
Manufacturer
Agilent Technologies
Industries
Energy & Chemicals , Materials Testing

An advanced integrated GC-MS/MS and LC-MS/MS workflow for the comprehensive analysis of pesticide residues in food

Applications
| 2024 | Thermo Fisher Scientific
Instrumentation
GC/MSD, GC/MS/MS, GC/QQQ, LC/MS, LC/MS/MS, LC/QQQ
Manufacturer
Thermo Fisher Scientific
Industries
Food & Agriculture
 

Related articles


Article | Upcoming events

Cal State LA hosts 15th Multidimensional Chromatography Workshop

This January, Cal State LA became a hub for scientists and researchers as it hosted the 15th Multidimensional Chromatography Workshop.
Complex Chemical Composition Analysis Lab (C³AL)
more

Article | Academy

Free NIST Data Processing Software Lab for Universities - Part 10: AMDIS Difficulties and Ways to Overcome Them in Free Laboratory Resource for Universities

In the tenth part, we will look at difficulties with AMDIS and ways to overcome them
James Little/Mass Spec Interpretation Services
more

Article | Scientific article

An innovative approach to planar chiral substances

New highly efficient method for the preparation of planar chiral compounds based on organocatalytic desymmetrization was published in Nature Communications
Katedra organické chemie Přírodovědecké fakulty Univerzity Karlovy
more

Article | Software

From a scientific project to a global business venture. The company mzio is striving to improve data analysis in mass spectrometry labs

A piece of software which enables researchers worldwide to speed up their work and helps them make scientific discoveries is expanding from science into commerce.
Ústav organické chemie a biochemie AV ČR
more
Other projects
Follow us
More information
WebinarsAbout usContact usTerms of use
LabRulez s.r.o. All rights reserved. Content available under a CC BY-SA 4.0 Attribution-ShareAlike