6th STARSS Conference on Separation Science (Program)

STARSS project is approaching its end after more than five years. A few more months remain before it will be concluded. The major objective of the project was to create a strong team of researchers enabling significant progress in the field of the separation science. The annual STARSS conferences have always been a good opportunity to bring people from several Czech and European institutions in Hradec Kralove where we have met face to face for a few days in order to inform each other about the progress in the research. It is clear that the focus of these conferences has been on the exchange in scientific information. However, an integral part of our previous conferences has also included informal meetings when we had time to talk about issues sometimes even completely outside of science perhaps with a drink and food. Social part of conferences is simply indispensable.

I trust that this conference will be at least as interesting as all the previous ones. Its program is rich and includes presentations by renowned “icons” of separation science from both abroad and the Czech Republic, as well as talks given by students and postdocs. Such “cross-pollination” will certainly be uplifting for all involved. I wish all those who attend this conference a pleasant stay in Hradec Králové and enjoyable conferencing.

Prof. František Švec

Program: 6th STARSS Conference on Separation Science

• Conference booklet including speakers bio

DAY 1: Monday 17. 10. 2022

08:00 – 09:00 REGISTRATION

09:00 – 09:20 Welcoming address

• Prof. Petr Solich, Prof. František Švec, Prof. Lucie Nováková
• Assoc. Prof. Jaroslav Roh (the dean of the Faculty of Pharmacy, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic)

SESSION I: IMPORTANCE OF SEPARATION SCIENCE FOR CLINICAL APPLICATIONS

• Chairperson: Prof. František Švec

09:20 – 10:00 L-01: UHPSFC/MS as a powerful tool for high-throughput lipidomic quantitation in cancer screening

• Prof. Michal Holčapek (University of Pardubice, Czech Republic)

10:00 – 10:40 L-02: Selected ion flow tube mass spectrometry, SIFT-MS, analyses of volatile metabolites in exhaled breath

• Prof. Patrik Španěl (J. Heyrovský Institute of Physical Chemistry, Prague, Czech Republic)

10:40 – 11:10 Coffee-break and discussion

SESSION II: BIOPHARMACEUTICALS

• Chairperson: Prof. Lucie Nováková

11:10 – 11:50 L-03: Simplifying the characterization of complex biopharmaceutical products through the use of ultra-short columns or multi-isocratic elution mode

• Dr. Davy Guillarme (University of Geneva, Switzerland)

11:50 – 12:20 L-04: Analysis of therapeutic proteins by multidimensional LC: How far can we go?

• Dr. Valentina D ́Atri (University of Geneva, Switzerland)

12:20 – 12:40 L-05: pyroLC: online thermal decomposition of proteins as a tool for ultra-fast bottom-up proteomics

• Dr. Juraj Lenčo (Charles University, Hradec Králové, Czech Republic)

12:40 – 13:00 L-06: RPLC-UV and HILIC-UV characterization of home-made ramucirumab-based antibody-drug conjugate

• Denis Naplekov (Charles University, Hradec Králové, Czech Republic)

13:00 – 14:00 Lunch

SESSION III: 2D SEPARATIONS

• Chairperson: Assoc. Prof. Jiří Urban

14:00 – 14:40 L-07: What can we expect from LC x LC compared to conventional 1D-LC

• Prof. Sabine Heinisch (Université Claude Bernard Lyon 1, Lyon, France)

14:40 – 15:20 L-08: Modelling of analyte profiles and band broadening generated by interface loops used in multi-dimensional liquid chromatography

• Prof. Ken Broeckhoven (Vrije Universiteit Brussel, Belgium)

15:20 – 15:50 L-09: Off-line LC x SFC in the prospect of upgrading natural or recycled products

• Dr. Karine Faure (Université Claude Bernard Lyon 1, Lyon, France)

15:50 – 16:20 L-10: Two-dimensional liquid chromatographic separations of complex samples of industrial products and intermediates

• Assoc. Prof. Petr Česla (University of Pardubice, Czech Republic)

16:20 – 16:50 Coffee-break and discussion

SESSION IV: IMPORTANCE OF STEROIDS

• Chairperson: Prof. Marcela Segundo

16:50 – 17:30 L-11: An image of the future of steroid biochemistry

• Prof. Ruth Andrew (University of Edinburgh, UK)

17:30 – 18:00 L-12: Ecdysterone metabolism: A holistic view on compound identification for doping control

• Prof. Maria Kristina Parr (Freie Universität Berlin, Berlin, Germany)

18:00 – 18:30 L-13: New liquid chromatography-tandem mass spectrometry method for targeted profiling of steroids in mouse plasma

• Taťána Gazárková (Charles University, Hradec Králové, Czech Republic)

18:30 – 19:30 Welcome drink

DAY 2: Tuesday 18. 10. 2022

08:00 – 09:00 REGISTRATION

SESSION V: SUPERCRITICAL FLUID CHROMATOGRAPHY AND EXTRACTIONS

• Chairperson: Assoc. Prof. Radim Kučera

09:00 – 09:30 L-14: Supercritical fluid extraction and chromatography in life science applications

• Prof. Charlotta Turner (Lund University, Lund, Sweden)

09:30 – 10:00 L-15: Supercritical fluid chromatography – tandem mass spectrometry for analysis of vitamin D metabolites in biological samples

• Dr. Margareta Sandahl (Lund University, Lund, Sweden)

10:00 – 10:30 L-16: The effect of make-up solvent composition on responses in SFC-MS: Electrospray vs UniSpray

• Dr. Kateřina Plachká (Charles University, Hradec Králové, Czech Republic)

10:30 – 11:00 L-17: UHPSFC-MS/MS versus 2D-UHPLC-MS/MS in chiral separation of Propranolol and its Hydroxy Metabolites

• Lukas Harps (Freie Universität Berlin, Berlin, Germany)

11:00 – 11:30 Coffee-break and discussion

SESSION VI: COLUMN TECHNOLOGY

• Chairperson: Prof. Dalibor Šatínský

11:30 – 12:10 L-18: Hypercrosslinked monolithic stationary phases in proteomics

• Assoc. Prof. Jiří Urban (Masaryk University, Brno, Czech Republic)

12:10 – 12:40 L-19: Guidelines for tuning the macropore structure of monolithic columns for high-performance liquid chromatography

• Dr. Sebastiaan Eeltink (Vrije Universiteit Brussel, Belgium)

12:40 – 13:10 L-20: Strategies and chemistry in column development

• Assoc. Prof. Michal Kohout (University of Chemistry and Technology, Prague, Czech Republic)

13:10 – 14:30 Lunch

SESSION VII: RETENTION MECHANISMS AND STATIONARY PHASES

• Chairperson: Prof. Spas Kolev

14:30 – 15:00 L-21: Understanding the mechanism, advantages and limitations of hydrophilic interaction liquid chromatography

• Prof. David McCalley (University of West England, UK)

15:00 – 15:30 L-22: Study of molecular structures through retention mechanisms in liquid chromatography: New trends and applications

• Dr. Jean-Christophe Garrigues (Paul Sabatier University - Toulouse III, Toulouse, France)

15:30 – 16:00 L-23: Problems of stationary phases in SFC

• Prof. Lucie Nováková (Charles University, Hradec Králové, Czech Republic)

16:00 – 16:30 L-24: The chromatographic behaviour of pharmaceuticals on stationary phases based on metal oxides

• Assoc. Prof. Radim Kučera (Charles University, Hradec Králové, Czech Republic)

16:30 – 17:00 Coffee-break and discussion

SESSION VIII: SAMPLE PREPARATION AND APPLICATIONS

• Chairperson: Assoc. Prof. Hana Sklenářová

17:00 – 17:30 L-25: Glucosinolate profiling in Brassicaceae family: instrumental analysis and biological application

• Dr. Iva Pavlovic (Palacký University, Olomouc, Czech Republic)

17:30 – 18:00 L-26: Flow programming for the development of online solid-phase extraction

• Assoc. Prof. Petr Chocholouš (Charles University, Hradec Králové, Czech Republic)

18:00 – 18:30 L-27: Automated tandem homogeneous liquid-liquid extraction and centrifugation-less deproteination for complex sample analysis on HPLC

• Dr. Burkhard Horstkotte (Charles University, Hradec Králové, Czech Republic)

19:00 – 22:00 Conference dinner

DAY 3: Wednesday 19. 10. 2022

WORKSHOPS: FACULTY OF PHARMACY

08:00 – 10:30 W1: Supercritical fluid extraction of polar and nonpolar compounds from Eucalyptus sp.

• Laboratory 916, 8th floor, Faculty of Pharmacy
• Dr. Veronika Pilařová (Charles University, Hradec Králové, Czech Republic)

08:00 – 10:30 W2: MetAmino® Kit – simple and easy sample preparation method for your LC-MC or GC-MS analysis

• Student’s Laboratory, 3rd floor, Faculty of Pharmacy
• Ing. Jana Volková (Chromservis, Czech Republic)

10:30 – 11:00 Coffee-break and discussion

11:00 – 13:30 W3: Who will help you with sample prep and liquid handling?

• Student’s Laboratory, 4th floor, Faculty of Pharmacy
• Dr. Martina Riesová (Waters, Prague, Czech Republic)

13:30 – 14:30 Lunch

14:30 – 17:00 W4: Practical application of nanofibers for advanced extraction approaches in chromatography

• Student’s Laboratory no. 524, 4th floor, Faculty of Pharmacy
• Prof. Dalibor Šatínský, Dr. Ivona Lhotská (Charles University, Hradec Králové, Czech Republic)

17:00 – 18:00 Coffee-break and discussion